ViewGene
Locus Name:
GeneID:
Location:

GeneID:2895
TrivialName: PA14_00290
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00290
PAO1 Ortholog Locus:PA0025
Sequence Length:825
Protein Length:274
Start: 26732
Stop: 27556
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03415.1|AE004442_2 (AE004442) shikimate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 272/274 (99%), Positives = 273/274 (99%)
Sequence:
ATGGACCGCTACTGCGTATTCGGCAACCCCATCGGCCACAGCAAGTCGCCGCTGATCCACCGCCTGTTCGCC
GAGCAGACCGGCGAGGCGCTGGTCTATGACGCGCAGCTGGCGCCGCTGGACGATTTCCCCGGGTTCGCCCGG
CGCTTCTTCGAGCAGGGCAAGGGCGCCAATGTCACCGTGCCGTTCAAGGAAGAGGCCTATCGTCTGGTGGAC
GAATTGAGCGAGCGGGCCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTGGCCGACGGTCGCCTGCGC
GGCGACAACACCGACGGCGCGGGCTTGCTGCGGGACCTGACGGCGAACGCCGGGGTCGAGCTGCGCGGCAAG
CGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGCGGGGTGCTCGAACCCTTCCTCGGCGAGTGCCCGGCG
GAGTTGCTGATCGCCAACCGCACGGCGCGGAAGGCCGTGGACCTGGCCGAGCGATTCGCCGATCTCGGCGCG
GTGCGCGGCTGCGGTTTCGCCGAGGTCGAAGGGCCTTTCGACCTGGTCGTCAACGGCACCTCGGCCAGTCTT
GCCGGCGACGTGCCGCCGCTGGCGCAGAGCGTGATCGAGCCCGGCCGTACCGTCTGCTACGACATGATGTAT
GCCAAGGAACCGACCGCCTTCAACCGCTGGGCCGCCGAACGCGGTGCGGCGCGTACCCTGGATGGCCTGGGC
ATGCTGGTGGAGCAGGCCGCCGAGGCATTCTTCCTCTGGCGTGGCGTGCGTCCTGCCTCGGCGCCGGTGTTG
GAGACGCTGCGCCGGCAATTGGCAACTGTCTGA
Translation:
MDRYCVFGNPIGHSKSPLIHRLFAEQTGEALVYDAQLAPLDDFPGFARRFFEQGKGANVTVPFKEEAYRLVD
ELSERATRAGAVNTLIRLADGRLRGDNTDGAGLLRDLTANAGVELRGKRVLLLGAGGAVRGVLEPFLGECPA
ELLIANRTARKAVDLAERFADLGAVRGCGFAEVEGPFDLVVNGTSASLAGDVPPLAQSVIEPGRTVCYDMMY
AKEPTAFNRWAAERGAARTLDGLGMLVEQAAEAFFLWRGVRPASAPVLETLRRQLATV*
AnnotationID:2903GeneID:2895
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 13:01:38
Gene Name:aroE
Confidence Code:2
GeneProduct:shikimate dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0169,pfam01488
Secondary Functional Category(ies):
EC Number:1.1.1.25
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53723.1| (AE016856) shikimate 5-dehydrogenase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 274

 Score =  384 bits (985), Expect = e-106
 Identities = 193/272 (70%), Positives = 219/272 (80%)

>gb|AAN65708.1|AE016197_6 (AE016774) shikimate 5-dehydrogenase [Pseudomonas putida KT2440]
          Length = 274

 Score =  383 bits (984), Expect = e-106
 Identities = 191/272 (70%), Positives = 219/272 (80%)

>gb|AAF93234.1| (AE004096) shikimate 5-dehydrogenase [Vibrio cholerae]
          Length = 278

 Score =  292 bits (747), Expect = 2e-78
 Identities = 149/272 (54%), Positives = 191/272 (70%), Gaps = 3/272 (1%)






 
Structural Features:
COG0169, AroE, Shikimate 5-dehydrogenase 
[Amino acid transport and metabolism]
CD-Length = 283 residues,  97.5% aligned
Score =  270 bits (691), Expect = 1e-73
Identities = 119/277 (42%), Positives = 150/277 (54%), Gaps = 7/277 (2%)

pfam01488, Shikimate_DH, Shikimate / quinate 5-dehydrogenase.
CD-Length = 240 residues, 100.0% aligned
Score =  258 bits (661), Expect = 5e-70
Identities = 119/241 (49%), Positives = 141/241 (58%), Gaps = 5/241 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5793




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22935




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33393




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2895_cdd.html


    		




 
URL:

















    ReferenceID:35518




  
Author/Investigator(s):
        Anton IA, Coggins JR.





  
Title:
        Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.




  
PubMed:
        3277621


MedLine:
        




    
Source:
Biochem J. 1988 Jan 15;249(2):319-26.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3277621

















    ReferenceID:35519




  
Author/Investigator(s):
        Henner DJ, Band L, Flaggs G, Chen E.





  
Title:
        The organization and nucleotide sequence of the Bacillus subtilis hisH, tyrA and aroE genes.




  
PubMed:
        3106153


MedLine:
        




    
Source:
Gene. 1986;49(1):147-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3106153









           
Homologs By Global Alignment 

           

       



     
Gene ID:2895
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4690
                           PA0025_tr
                           translation of PA0025
                           274
                           99.27
                           99.63
                           0
                           1396.0
     

     
     
134853
                           gb|AAG03415.1|AE004442_2
                           (AE004442) shikimate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           274
                           99.27
                           99.63
                           0
                           1396.0
     

     
     
134854
                           emb|CAA59377.1|
                           (X85015) shikimate 5-dehydrogenase [Pseudomonas aeruginosa]
                           274
                           98.54
                           99.63
                           0
                           1390.0
     

     
     
134855
                           gb|AAO53723.1|
                           (AE016856) shikimate 5-dehydrogenase [Pseudomonas syringae pv. tomato str. DC3000]
                           274
                           70.43
                           79.92
                           0
                           982.0
     

     
     
134856
                           gb|AAN65708.1|AE016197_6
                           (AE016774) shikimate 5-dehydrogenase [Pseudomonas putida KT2440]
                           274
                           69.70
                           79.92
                           0
                           980.0
     

     






      

          
GC ORFID: 45897How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0025Glimmer Score: 

          
Start: 5828069Stop: 5828893

          
Length: 825


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0025 translation of PA0025Homolog Bit Score 542.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2985 (ORF: BLASTX 381.0), t_PA0025 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGACCGCTACTGCGTATTCGGCAACCCCATCGGCCACAGCAAGTCGCCGCTGATCCACCGCCTGTTCGCC 
GAGCAGACCGGCGAGGCGCTGGTCTATGACGCGCAGCTGGCGCCGCTGGACGATTTCCCCGGGTTCGCCCGG 
CGCTTCTTCGAGCAGGGCAAGGGCGCCAATGTCACCGTGCCGTTCAAGGAAGAGGCCTATCGTCTGGTGGAC 
GAATTGAGCGAGCGGGCCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTGGCCGACGGTCGCCTGCGC 
GGCGACAACACCGACGGCGCGGGCTTGCTGCGGGACCTGACGGCGAACGCCGGGGTCGAGCTGCGCGGCAAG 
CGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGCGGGGTGCTCGAACCCTTCCTCGGCGAGTGCCCGGCG 
GAGTTGCTGATCGCCAACCGCACGGCGCGGAAGGCCGTGGACCTGGCCGAGCGATTCGCCGATCTCGGCGCG 
GTGCGCGGCTGCGGTTTCGCCGAGGTCGAAGGGCCTTTCGACCTGGTCGTCAACGGCACCTCGGCCAGTCTT 
GCCGGCGACGTGCCGCCGCTGGCGCAGAGCGTGATCGAGCCCGGCCGTACCGTCTGCTACGACATGATGTAT 
GCCAAGGAACCGACCGCCTTCAACCGCTGGGCCGCCGAACGCGGTGCGGCGCGTACCCTGGATGGCCTGGGC 
ATGCTGGTGGAGCAGGCCGCCGAGGCATTCTTCCTCTGGCGTGGCGTGCGTCCTGCCTCGGCGCCGGTGTTG 
GAGACGCTGCGCCGGCAATTGGCAACTGTCTGA