ViewGene
Locus Name:
GeneID:
Location:

GeneID:2894
TrivialName: PA14_14960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14960
PAO1 Ortholog Locus:PA3797
Sequence Length:795
Protein Length:264
Start: 1270228
Stop: 1271022
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07184.1|AE004798_7 (AE004798) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 263/264 (99%), Positives = 263/264 (99%)
Sequence:
ATGCGCGATCTGACGGTTCTGCCGGACCTGGACATCGCCCTGGTCCAATCCTCCCTGGCCTGGCACGACGCC
CAGGCCAACCGCGAGCATTTCGCCGCCCTGCTGGAGAGCGCCGCCGGCGCCGACCTGGTGGTCCTCCCGGAG
ATGTTCACCACGGGTTTCAGCATGGCTTCGGCCGAGCAGGCCGAGCCCGAGCTGGGGCCGACCCATGCCTGG
CTGCTGGAGCAGGCCCGGCGCCTTGGTGCGGTGGTCACCGGCAGCCTGATCGTCCAGCTCGCCGACGGTAGC
CATCGCAATCGCCTGCTCTGGGCGCGCCCGGACGGCGAGATGCTGCACTACGACAAGCGCCATCTGTTCCGC
ATGGCCGGCGAACACGAGCACTACAGTCCCGGCGAACGCCAGGAACTGTTCGAACTCAAGGGCTGGCGGGTG
CGCCCGTTGATCTGCTACGACCTGCGTTTCCCGGTGTGGAGCCGCGACCCGCACGACACCGACCTGTTGCTC
TACACCGCCAACTGGCCCGCGCCTCGTCGACAGCACTGGAACCGCCTGCTGCCGGCGCGGGCGATCGAGAAC
CTTTGCTACGTGGTGGCGGTGAACCGTATCGGCGAAGACGGCAACGCCTTGCGCTACGCTGGGGATAGCCAG
GTCCTGGACTTCCAGGGCGACAGCCTGTTCAACGCGGCGGACGCCGACGGCGTGTTCCGCGTCCGGCTGGAC
GCGGCGGCACTGGCGGCCTATCGCCAGCGTTTCCCGGCCTACATGGACGCGGATCGCTTCGAAATCCATCCT
TGA
Translation:
MRDLTVLPDLDIALVQSSLAWHDAQANREHFAALLESAAGADLVVLPEMFTTGFSMASAEQAEPELGPTHAW
LLEQARRLGAVVTGSLIVQLADGSHRNRLLWARPDGEMLHYDKRHLFRMAGEHEHYSPGERQELFELKGWRV
RPLICYDLRFPVWSRDPHDTDLLLYTANWPAPRRQHWNRLLPARAIENLCYVVAVNRIGEDGNALRYAGDSQ
VLDFQGDSLFNAADADGVFRVRLDAAALAAYRQRFPAYMDADRFEIHP*
AnnotationID:2902GeneID:2894
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 10:10:02
Gene Name:
Confidence Code:3
GeneProduct:putative amidohydrolase
Cell Localization:()
Synonyms:yafV
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:putative carbon-nitrogen hydrolase
Functional Category Confidence Code:5
COGs:COG0388,pfam00795,KOG0806
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66484.1|AE016277_3 (AE016777) carbon-nitrogen hydrolase family protein [Pseudomonas
           putida KT2440]
          Length = 263

 Score =  413 bits (1062), Expect = e-115
 Identities = 193/263 (73%), Positives = 217/263 (82%)

>gb|AAO29317.1| (AE012558) hydrolase [Xylella fastidiosa Temecula1]
          Length = 270

 Score =  249 bits (636), Expect = 1e-65
 Identities = 133/266 (50%), Positives = 165/266 (62%), Gaps = 10/266 (3%)

>gb|AAC73323.1| (AE000130) putative EC 3.5. amidase-type enzyme [Escherichia coli
           K12]
          Length = 256

 Score =  248 bits (634), Expect = 2e-65
 Identities = 126/258 (48%), Positives = 163/258 (63%), Gaps = 5/258 (1%)






 
Structural Features:
COG0388, Predicted amidohydrolase 
CD-Length = 274 residues,  96.0% aligned
Score =  174 bits (442), Expect = 2e-44
Identities = 92/264 (34%), Positives = 123/264 (46%), Gaps = 14/264 (5%)

pfam00795, CN_hydrolase, Carbon-nitrogen hydrolase.
CD-Length = 174 residues,  99.4% aligned
Score = 90.0 bits (223), Expect = 6e-19
Identities = 57/173 (32%), Positives = 85/173 (49%), Gaps = 12/173 (6%)

KOG0806, Carbon-nitrogen hydrolase [Amino acid transport and metabolism]
CD-Length = 298 residues,  92.3% aligned
Score = 80.3 bits (198), Expect = 4e-16
Identities = 69/279 (24%), Positives = 106/279 (37%), Gaps = 33/279 (11%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5791




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22933




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31762




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2894_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2894
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4689
                           PA3797_tr
                           translation of PA3797
                           264
                           99.62
                           99.62
                           0
                           1397.0
     

     
     
134833
                           gb|AAG07184.1|AE004798_7
                           (AE004798) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           264
                           99.62
                           99.62
                           0
                           1397.0
     

     
     
134834
                           gb|AAN66484.1|AE016277_3
                           (AE016777) carbon-nitrogen hydrolase family protein [Pseudomonas putida KT2440]
                           264
                           73.10
                           82.19
                           1
                           1062.0
     

     
     
134835
                           gb|AAO54962.1|
                           (AE016860) hydrolase, carbon-nitrogen family [Pseudomonas syringae pv. tomato str. DC3000]
                           264
                           72.34
                           81.06
                           1
                           1045.0
     

     
     
134837
                           gb|AAM37174.1|
                           (AE011869) hydrolase [Xanthomonas axonopodis pv. citri str. 306]
                           274
                           50.00
                           60.94
                           17
                           670.0
     

     






      

          
GC ORFID: 45896How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3797Glimmer Score: 

          
Start: 3683710Stop: 3682916

          
Length: 795


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3797 translation of PA3797Homolog Bit Score 542.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4534 (ORF: BLASTX 409.0), t_PA3797 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCGATCTGACGGTTCTGCCGGACCTGGACATCGCCCTGGTCCAATCCTCCCTGGCCTGGCACGACGCC 
CAGGCCAACCGCGAGCATTTCGCCGCCCTGCTGGAGAGCGCCGCCGGCGCCGACCTGGTGGTCCTCCCGGAG 
ATGTTCACCACGGGTTTCAGCATGGCTTCGGCCGAGCAGGCCGAGCCCGAGCTGGGGCCGACCCATGCCTGG 
CTGCTGGAGCAGGCCCGGCGCCTTGGTGCGGTGGTCACCGGCAGCCTGATCGTCCAGCTCGCCGACGGTAGC 
CATCGCAATCGCCTGCTCTGGGCGCGCCCGGACGGCGAGATGCTGCACTACGACAAGCGCCATCTGTTCCGC 
ATGGCCGGCGAACACGAGCACTACAGTCCCGGCGAACGCCAGGAACTGTTCGAACTCAAGGGCTGGCGGGTG 
CGCCCGTTGATCTGCTACGACCTGCGTTTCCCGGTGTGGAGCCGCGACCCGCACGACACCGACCTGTTGCTC 
TACACCGCCAACTGGCCCGCGCCTCGTCGACAGCACTGGAACCGCCTGCTGCCGGCGCGGGCGATCGAGAAC 
CTTTGCTACGTGGTGGCGGTGAACCGTATCGGCGAAGACGGCAACGCCTTGCGCTACGCTGGGGATAGCCAG 
GTCCTGGACTTCCAGGGCGACAGCCTGTTCAACGCGGCGGACGCCGACGGCGTGTTCCGCGTCCGGCTGGAC 
GCGGCGGCACTGGCGGCCTATCGCCAGCGTTTCCCGGCCTACATGGACGCGGATCGCTTCGAAATCCATCCT 
TGA