ViewGene
Locus Name:
GeneID:
Location:

GeneID:2891
TrivialName: PA14_58660
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58660
PAO1 Ortholog Locus:PA4521
Sequence Length:837
Protein Length:278
Start: 5228483
Stop: 5227647
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07909.1|AE004866_3 (AE004866) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 276/278 (99%), Positives = 277/278 (99%)
Sequence:
ATGACGTTCCTGGTGTTGCTGCTGGCTCTTGCGCTGGAGAAGTTCTCCGGCTGGCGCCGGCGTATCCAGCGC
GATGGCGCCTGGCTCGCCTGGCTGAGCCTGGTCGAGGACCTGCCCTCGGTGTCCCGCCGCTCCTGGCTGGGG
CTGCTGCTCGCGGTAGTCCTGCCGTTGCTGCCGCTGGCCCTGCTGCTGACATTGCTGCAGCCGCTGGCCTAT
GGCTGGCTGAGTCTTCCGCTGCATCTTCTGGTGGTGGTCTACAGTCTCGGCCGCGGCGACGTCATGGCCGAC
CTCGGGCCGTTCCGCGACGCCTGGCGGCGCGAGGAGGCCCAGGCTGCCTTCCATGTCGCCGAACGCGATCTG
GGGATCCTGGGCGGCAGCGAGAGCGACCTGATCGGCCGGGTGCACGGCCATCTGCTCTGGCAGGGCTACCAG
AGCTTCTTCGCGGTGATCTTCTGGTATGCCTTGCTCGGCCCGGTGGCGGCCCTGGCCTATCGCCTGCTGGCA
CTGGCGCTGGAGCACGCCCGCCAGCCGGCGTTGCGCGAACAGGCGGCGCGCGTCCGGCATATCCTCGACTGG
TTGCCGGTGCGTGCGCTGGTGCTGAGCTTCGCCCTGGTCGGCAACTTCCTCGCGGTTATCCGCGTGCTGCTG
CACTGGCTGCTGGCCTGGGACATCTCGGCGGCCGCGCTGCTGGGCAAGGCCGGACGGGTCGCCAGCGATGTC
GAAGCGGTCGAACTGGGTGCCGCCGGCGTCGCCAGCCTGGATGCGCTCTGGCAATTGCTGGTGCGTGCGGCC
GTGCTCTGGTACGCCGTGTTCGCCTTCTGCGCGCTGTTCCTCTGA
Translation:
MTFLVLLLALALEKFSGWRRRIQRDGAWLAWLSLVEDLPSVSRRSWLGLLLAVVLPLLPLALLLTLLQPLAY
GWLSLPLHLLVVVYSLGRGDVMADLGPFRDAWRREEAQAAFHVAERDLGILGGSESDLIGRVHGHLLWQGYQ
SFFAVIFWYALLGPVAALAYRLLALALEHARQPALREQAARVRHILDWLPVRALVLSFALVGNFLAVIRVLL
HWLLAWDISAAALLGKAGRVASDVEAVELGAAGVASLDALWQLLVRAAVLWYAVFAFCALFL*
AnnotationID:2899GeneID:2891
AnnotatorUID: diggins
Modification Date/Time: 2005-05-04 15:03:04
Gene Name:
Confidence Code:3
GeneProduct:transmembrane protein
Cell Localization:()
Synonyms:ampE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3725
Secondary Functional Category(ies):defense mechanisms
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC98784.1| (AF082575) transmembrane protein AmpE [Pseudomonas aeruginosa]
          Length = 278

 Score =  543 bits (1400), Expect = e-154
 Identities = 276/278 (99%), Positives = 277/278 (99%)


>gb|AAO54485.1| (AE016859) ampE protein [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 278

 Score =  316 bits (809), Expect = 1e-85
 Identities = 159/276 (57%), Positives = 204/276 (73%)








 
Structural Features:
COG3725, AmpE, Membrane protein required for beta-lactamase induction 
[Defense mechanisms]
CD-Length = 282 residues,  98.9% aligned
Score =  262 bits (672), Expect = 5e-71
Identities = 129/282 (45%), Positives = 156/282 (55%), Gaps = 7/282 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5785




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22927




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31568




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2891_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2891
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4686
                           PA4521_tr
                           translation of PA4521
                           278
                           99.28
                           99.64
                           0
                           1400.0
     

     
     
134822
                           gb|AAG07909.1|AE004866_3
                           (AE004866) hypothetical protein PA4521 [Pseudomonas aeruginosa PAO1]
                           278
                           99.28
                           99.64
                           0
                           1400.0
     

     
     
134823
                           gb|AAC98784.1|
                           (AF082575) transmembrane protein AmpE [Pseudomonas aeruginosa]
                           278
                           99.28
                           99.64
                           0
                           1400.0
     

     
     
134826
                           gb|AAF73951.1|AF237388_2
                           (AF237388) AmpE [Azotobacter vinelandii]
                           281
                           58.36
                           70.81
                           4
                           770.0
     

     
     
134825
                           gb|AAN66415.1|AE016269_2
                           (AE016777) inner membrane protein AmpE [Pseudomonas putida KT2440]
                           278
                           57.55
                           72.66
                           2
                           799.0
     

     






      

          
GC ORFID: 45893How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4521Glimmer Score: 

          
Start: 4492162Stop: 4491326

          
Length: 837


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4521 translation of PA4521Homolog Bit Score 543.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2493 (ORF: BLASTX 332.0), t_PA4521 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGTTCCTGGTGTTGCTGCTGGCTCTTGCGCTGGAGAAGTTCTCCGGCTGGCGCCGGCGTATCCAGCGC 
GATGGCGCCTGGCTCGCCTGGCTGAGCCTGGTCGAGGACCTGCCCTCGGTGTCCCGCCGCTCCTGGCTGGGG 
CTGCTGCTCGCGGTAGTCCTGCCGTTGCTGCCGCTGGCCCTGCTGCTGACATTGCTGCAGCCGCTGGCCTAT 
GGCTGGCTGAGTCTTCCGCTGCATCTTCTGGTGGTGGTCTACAGTCTCGGCCGCGGCGACGTCATGGCCGAC 
CTCGGGCCGTTCCGCGACGCCTGGCGGCGCGAGGAGGCCCAGGCTGCCTTCCATGTCGCCGAACGCGATCTG 
GGGATCCTGGGCGGCAGCGAGAGCGACCTGATCGGCCGGGTGCACGGCCATCTGCTCTGGCAGGGCTACCAG 
AGCTTCTTCGCGGTGATCTTCTGGTATGCCTTGCTCGGCCCGGTGGCGGCCCTGGCCTATCGCCTGCTGGCA 
CTGGCGCTGGAGCACGCCCGCCAGCCGGCGTTGCGCGAACAGGCGGCGCGCGTCCGGCATATCCTCGACTGG 
TTGCCGGTGCGTGCGCTGGTGCTGAGCTTCGCCCTGGTCGGCAACTTCCTCGCGGTTATCCGCGTGCTGCTG 
CACTGGCTGCTGGCCTGGGACATCTCGGCGGCCGCGCTGCTGGGCAAGGCCGGACGGGTCGCCAGCGATGTC 
GAAGCGGTCGAACTGGGTGCCGCCGGCGTCGCCAGCCTGGATGCGCTCTGGCAATTGCTGGTGCGTGCGGCC 
GTGCTCTGGTACGCCGTGTTCGCCTTCTGCGCGCTGTTCCTCTGA