ViewGene
Locus Name:
GeneID:
Location:

GeneID:2883
TrivialName: PA14_45640
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45640
PAO1 Ortholog Locus:PA1454
Sequence Length:843
Protein Length:280
Start: 4060258
Stop: 4059416
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04843.1|AE004575_2 (AE004575) flagellar synthesis regulator FleN [Pseudomonas aeruginosa PAO1]
Identities = 280/280 (100%), Positives = 280/280 (100%)
Sequence:
ATGAAGCAGATGGGTAGCATGCATCCCGTACAGGTGATCGCAGTAACTGGCGGCAAGGGCGGCGTCGGCAAG
ACCAATGTGTCGGTGAACCTGGCACTGGCTCTGGCCGATCTCGGCCGTCGCGTCATGCTGCTGGATGCCGAC
CTCGGCCTGGCCAACGTCGACGTCCTGCTGGGACTCACGCCCAAGCGCACCCTGGCCGACGTGATCGAGGGA
CGCTGCGAACTGCGCGATGTCCTCCTCCTCGGTCCCGGTGGCGTGCGCATCGTTCCCGCGGCCTCCGGCACG
CAGAGCATGGTGCACCTCTCGCCGATGCAGCATGCGGGGCTGATCCAGGCGTTCAGCGATATCAGCGACAAC
CTCGATGTCCTGGTCGTCGACACTGCCGCCGGAATCGGCGATTCGGTGGTCAGTTTCGTCCGCGCCGCGCAG
GAGGTGCTGCTGGTCGTCTGCGACGAGCCGACCTCGATCACCGATGCCTACGCCCTGATCAAGCTGCTCAAC
CGCGACCACGGCATGACCCGCTTCCGGGTCCTGGCCAACATGGCCCACAGCCCGCAGGAAGGACGCAACCTG
TTCGCCAAACTGACCAAGGTCACTGACCGTTTCCTCGACGTCGCACTACAGTATGTAGGTGTCATCCCTTAC
GACGAGTCGGTACGCAAGGCCGTGCAGAAACAGCGAGCCGTATACGAGGCATTCCCCCGCTCGAAGGCGTCC
CTGGCCTTCAAGGCGGTCGCGCAGAAGGTCGACAGCTGGCCGCTGCCGGCCAACCCGCGCGGGCACCTGGAG
TTCTTCGTCGAACGACTGGTCCAACACCCCGCGACAGGTTCGGCCGTATGA
Translation:
MKQMGSMHPVQVIAVTGGKGGVGKTNVSVNLALALADLGRRVMLLDADLGLANVDVLLGLTPKRTLADVIEG
RCELRDVLLLGPGGVRIVPAASGTQSMVHLSPMQHAGLIQAFSDISDNLDVLVVDTAAGIGDSVVSFVRAAQ
EVLLVVCDEPTSITDAYALIKLLNRDHGMTRFRVLANMAHSPQEGRNLFAKLTKVTDRFLDVALQYVGVIPY
DESVRKAVQKQRAVYEAFPRSKASLAFKAVAQKVDSWPLPANPRGHLEFFVERLVQHPATGSAV*
AnnotationID:2891GeneID:2883
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 12:12:33
Gene Name:fleN
Confidence Code:1
GeneProduct:flagellar synthesis regulator FleN
Cell Localization:()
Synonyms:motR,flhG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0455,COG2894
Secondary Functional Category(ies):cell division and chromosome partitioning
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69921.1|AE016630_12 (AE016790) flagellar number regulator FleN [Pseudomonas putida
           KT2440]
          Length = 277

 Score =  496 bits (1277), Expect = e-140
 Identities = 252/274 (91%), Positives = 263/274 (95%)

>gb|AAO55496.1| (AE016862) flagellar synthesis regulator FleN [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 274

 Score =  488 bits (1257), Expect = e-137
 Identities = 248/271 (91%), Positives = 259/271 (95%)

>gb|AAN56210.1|AE015759_2 (AE015759) flagellar biosynthetic protein FlhG [Shewanella
           oneidensis MR-1]
          Length = 283

 Score =  349 bits (895), Expect = 1e-95
 Identities = 169/265 (63%), Positives = 220/265 (83%)






 
Structural Features:
COG0455, COG0455, ATPases involved in chromosome partitioning 
[Cell division and chromosome partitioning]
CD-Length = 262 residues,  99.6% aligned
Score =  217 bits (554), Expect = 1e-57
Identities = 96/269 (35%), Positives = 151/269 (56%), Gaps = 12/269 (4%)

COG2894, MinD, Septum formation inhibitor-activating ATPase 
[Cell division and chromosome partitioning]
CD-Length = 272 residues,  89.3% aligned
Score =  103 bits (258), Expect = 3e-23
Identities = 72/250 (28%), Positives = 124/250 (49%), Gaps = 17/250 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5769




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22912




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37379




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2883_cdd.html


    		




 
URL:

















    ReferenceID:37965




  
Author/Investigator(s):
        Dasgupta N, Arora SK, Ramphal R.





  
Title:
        fleN, a gene that regulates flagellar number in Pseudomonas aeruginosa.




  
PubMed:
        10629180


MedLine:
        




    
Source:
J Bacteriol. 2000 Jan;182(2):357-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10629180

















    ReferenceID:37966




  
Author/Investigator(s):
        Dasgupta N, Wolfgang MC, Goodman AL, Arora SK, Jyot J, Lory S, Ramphal R.





  
Title:
        A four-tiered transcriptional regulatory circuit controls flagellar biogenesis in Pseudomonas aeruginosa.




  
PubMed:
        14617143


MedLine:
        




    
Source:
Mol Microbiol. 2003 Nov;50(3):809-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14617143









           
Homologs By Global Alignment 

           

       



     
Gene ID:2883
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4677
                           PA1454_tr
                           translation of PA1454
                           280
                           99.99
                           99.99
                           0
                           1400.0
     

     
     
134747
                           gb|AAG04843.1|AE004575_2
                           (AE004575) flagellar synthesis regulator FleN [Pseudomonas aeruginosa PAO1]
                           280
                           99.99
                           99.99
                           0
                           1400.0
     

     
     
134748
                           gb|AAF24747.1|AF133657_1
                           (AF133657) flagellar number regulator [Pseudomonas aeruginosa]
                           280
                           99.99
                           99.99
                           0
                           1400.0
     

     
     
134749
                           gb|AAC62540.2|
                           (AF052586) MotR [Pseudomonas aeruginosa]
                           280
                           93.21
                           96.78
                           5
                           1305.0
     

     
     
134750
                           gb|AAN69921.1|AE016630_12
                           (AE016790) flagellar number regulator FleN [Pseudomonas putida KT2440]
                           280
                           90.00
                           94.28
                           3
                           1277.0
     

     






      

          
GC ORFID: 45885How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1454Glimmer Score: 

          
Start: 893651Stop: 894493

          
Length: 843


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1454 translation of PA1454Homolog Bit Score 543.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7938 (ORF: BLASTX 494.0), t_PA1454 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCAGATGGGTAGCATGCATCCCGTACAGGTGATCGCAGTAACTGGCGGCAAGGGCGGCGTCGGCAAG 
ACCAATGTGTCGGTGAACCTGGCACTGGCTCTGGCCGATCTCGGCCGTCGCGTCATGCTGCTGGATGCCGAC 
CTCGGCCTGGCCAACGTCGACGTCCTGCTGGGACTCACGCCCAAGCGCACCCTGGCCGACGTGATCGAGGGA 
CGCTGCGAACTGCGCGATGTCCTCCTCCTCGGTCCCGGTGGCGTGCGCATCGTTCCCGCGGCCTCCGGCACG 
CAGAGCATGGTGCACCTCTCGCCGATGCAGCATGCGGGGCTGATCCAGGCGTTCAGCGATATCAGCGACAAC 
CTCGATGTCCTGGTCGTCGACACTGCCGCCGGAATCGGCGATTCGGTGGTCAGTTTCGTCCGCGCCGCGCAG 
GAGGTGCTGCTGGTCGTCTGCGACGAGCCGACCTCGATCACCGATGCCTACGCCCTGATCAAGCTGCTCAAC 
CGCGACCACGGCATGACCCGCTTCCGGGTCCTGGCCAACATGGCCCACAGCCCGCAGGAAGGACGCAACCTG 
TTCGCCAAACTGACCAAGGTCACTGACCGTTTCCTCGACGTCGCACTACAGTATGTAGGTGTCATCCCTTAC 
GACGAGTCGGTACGCAAGGCCGTGCAGAAACAGCGAGCCGTATACGAGGCATTCCCCCGCTCGAAGGCGTCC 
CTGGCCTTCAAGGCGGTCGCGCAGAAGGTCGACAGCTGGCCGCTGCCGGCCAACCCGCGCGGGCACCTGGAG 
TTCTTCGTCGAACGACTGGTCCAACACCCCGCGACAGGTTCGGCCGTATGA