ViewGene
Locus Name:
GeneID:
Location:

GeneID:2881
TrivialName: PA14_67240
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67240
PAO1 Ortholog Locus:PA5091
Sequence Length:801
Protein Length:266
Start: 6005174
Stop: 6004374
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08476.1|AE004922_1 (AE004922) N-formylglutamate amidohydrolase [Pseudomonas aeruginosa PAO1]
Identities = 265/266 (99%), Positives = 265/266 (99%)
Sequence:
GTGGATGAAGTCCTGAGTTTCAAGCGCGGCCGCGTGCCGCTACTGATCAGCATGCCGCACCCCGGCACGCGC
CTGACCCCGGCGGTGGACGCCGGCCTGGTGGAGGAGGCGCGGGCGCTGACCGATACCGACTGGCACATTCCC
CGGCTCTACGATTTCGCCGAGGAACTGGGCGCCAGCACCCTGGCTGCCCACTATTCGCGCTATGTAGTCGAT
CTCAACCGCCCTTCCGACGACAAGCCGCTGTACAGCACCGCCACTACCGGCCTGTATCCGGACACCCTGTTC
GACGGCCGGCCGCTCTACCGCGAAGGCATGGCGCCGTCCGCCGAGGAACGCATGCGCTACCTGGCCGAAGTG
TGGACGCCCTACCACCGGACCATCGCCGAGGAACTGGCGCGGCTGAAGGCCGAGTTCGGCTACGCGCTGCTC
TGGGACGCCCACTCGATCCGTTCCCACGTGCCGCACCTGTTCGACGGCCGACTGCCCGACTTCAATCTCGGC
ACCAACGCCGGCGCCAGTTGCGATCCAGCGCTGGCGGCCCGCCTGGAGGCGGTCTGCGCTGCCGCCGAAGGC
TACAGCCATGTGCTCAACGGGCGCTTCAAGGGCGGCCACATCACCCGCCACTACGGCCAGCCGGAGCAGCAC
GTCCATGCCGTCCAGCTGGAGCTGGCGCAGTGCACCTACATGGACGAGCAGGCCCCGTTCGCTTACCGCGCG
GACCTCGCCGAAGCGACCCGCGCGGTCATCCGCGAACTGCTGGAAAGCCTCCTCGCCTGGGGGCACGAGCGC
TACGCCTGA
Translation:
MDEVLSFKRGRVPLLISMPHPGTRLTPAVDAGLVEEARALTDTDWHIPRLYDFAEELGASTLAAHYSRYVVD
LNRPSDDKPLYSTATTGLYPDTLFDGRPLYREGMAPSAEERMRYLAEVWTPYHRTIAEELARLKAEFGYALL
WDAHSIRSHVPHLFDGRLPDFNLGTNAGASCDPALAARLEAVCAAAEGYSHVLNGRFKGGHITRHYGQPEQH
VHAVQLELAQCTYMDEQAPFAYRADLAEATRAVIRELLESLLAWGHERYA*
AnnotationID:2889GeneID:2881
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 12:12:39
Gene Name:hutG
Confidence Code:2
GeneProduct:N-formylglutamate amidohydrolase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:formiminoglutamase
Functional Category Confidence Code:5
COGs:COG3741,pfam05013
Secondary Functional Category(ies):central intermediary metabolism
EC Number:3.5.3.8
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58704.1| (AE016875) formimidoylglutamase, putative [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 266

 Score =  417 bits (1073), Expect = e-116
 Identities = 194/265 (73%), Positives = 225/265 (84%)

>gb|AAB86969.1| (AF032970) N-formylglutamate amidohydrolase [Pseudomonas putida]
          Length = 267

 Score =  416 bits (1070), Expect = e-116
 Identities = 192/265 (72%), Positives = 225/265 (84%)

>emb|CAA21332.1| (AL031866) hutG [Yersinia pestis]
          Length = 268

 Score =  330 bits (845), Expect = 7e-90
 Identities = 155/259 (59%), Positives = 191/259 (73%)








 
Structural Features:
COG3741, HutG, N-formylglutamate amidohydrolase 
[Amino acid transport and metabolism]
CD-Length = 272 residues,  98.5% aligned
Score =  326 bits (836), Expect = 2e-90
Identities = 139/269 (51%), Positives = 171/269 (63%), Gaps = 4/269 (1%)

pfam05013, FGase, N-formylglutamate amidohydrolase. 
CD-Length = 222 residues, 100.0% aligned
Score =  253 bits (648), Expect = 1e-68
Identities = 118/228 (51%), Positives = 134/228 (58%), Gaps = 13/228 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14687




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22908




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34963




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2881_cdd.html


    		




 
URL:

















    ReferenceID:35818




  
Author/Investigator(s):
        Yoshida K, Sano H, Seki S, Oda M, Fujimura M, Fujita Y.





  
Title:
        Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome containing the hut and wapA loci.




  
PubMed:
        7704263


MedLine:
        




    
Source:
Microbiology. 1995 Feb;141 ( Pt 2):337-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7704263

















    ReferenceID:35819




  
Author/Investigator(s):
        Schwacha A, Bender RA.





  
Title:
        Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Klebsiella aerogenes.




  
PubMed:
        2203754


MedLine:
        




    
Source:
J Bacteriol. 1990 Sep;172(9):5477-81.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2203754









           
Homologs By Global Alignment 

           

       



     
Gene ID:2881
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4675
                           PA5091_tr
                           translation of PA5091
                           266
                           99.62
                           99.62
                           0
                           1402.0
     

     
     
134710
                           gb|AAG08476.1|AE004922_1
                           (AE004922) N-formylglutamate amidohydrolase [Pseudomonas aeruginosa PAO1]
                           266
                           99.24
                           99.62
                           0
                           1399.0
     

     
     
134711
                           gb|AAO58704.1|
                           (AE016875) formimidoylglutamase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           266
                           72.55
                           84.58
                           0
                           1069.0
     

     
     
134712
                           gb|AAB86969.1|
                           (AF032970) N-formylglutamate amidohydrolase [Pseudomonas putida]
                           267
                           71.53
                           84.26
                           1
                           1065.0
     

     
     
134713
                           gb|AAN70594.1|AE016702_3
                           (AE016792) formiminoglutamase [Pseudomonas putida KT2440]
                           267
                           70.78
                           83.89
                           1
                           1049.0
     

     






      

          
GC ORFID: 45883How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5091Glimmer Score: 

          
Start: 5268859Stop: 5268059

          
Length: 801


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5091 translation of PA5091Homolog Bit Score 544.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6907 (ORF: BLASTX 416.0), t_PA5091 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGATGAAGTCCTGAGTTTCAAGCGCGGCCGCGTGCCGCTACTGATCAGCATGCCGCACCCCGGCACGCGC 
CTGACCCCGGCGGTGGACGCCGGCCTGGTGGAGGAGGCGCGGGCGCTGACCGATACCGACTGGCACATTCCC 
CGGCTCTACGATTTCGCCGAGGAACTGGGCGCCAGCACCCTGGCTGCCCACTATTCGCGCTATGTAGTCGAT 
CTCAACCGCCCTTCCGACGACAAGCCGCTGTACAGCACCGCCACTACCGGCCTGTATCCGGACACCCTGTTC 
GACGGCCGGCCGCTCTACCGCGAAGGCATGGCGCCGTCCGCCGAGGAACGCATGCGCTACCTGGCCGAAGTG 
TGGACGCCCTACCACCGGACCATCGCCGAGGAACTGGCGCGGCTGAAGGCCGAGTTCGGCTACGCGCTGCTC 
TGGGACGCCCACTCGATCCGTTCCCACGTGCCGCACCTGTTCGACGGCCGACTGCCCGACTTCAATCTCGGC 
ACCAACGCCGGCGCCAGTTGCGATCCAGCGCTGGCGGCCCGCCTGGAGGCGGTCTGCGCTGCCGCCGAAGGC 
TACAGCCATGTGCTCAACGGGCGCTTCAAGGGCGGCCACATCACCCGCCACTACGGCCAGCCGGAGCAGCAC 
GTCCATGCCGTCCAGCTGGAGCTGGCGCAGTGCACCTACATGGACGAGCAGGCCCCGTTCGCTTACCGCGCG 
GACCTCGCCGAAGCGACCCGCGCGGTCATCCGCGAACTGCTGGAAAGCCTCCTCGCCTGGGGGCACGAGCGC 
TACGCCTGA