ViewGene
Locus Name:
GeneID:
Location:

GeneID:2880
TrivialName: PA14_66220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66220
PAO1 Ortholog Locus:PA5009
Sequence Length:807
Protein Length:268
Start: 5900068
Stop: 5899262
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08394.1|AE004913_9 (AE004913) lipopolysaccharide kinase WaaP [Pseudomonas aeruginosa PAO1]
Identities = 267/268 (99%), Positives = 268/268 (100%)
Sequence:
ATGAGGCTGGTGCTGGAAGAGCCGTTCAAGCGCCTGTGGAGCGGGCGCGACCCGTTCGAGGCGGTGGAGGCG
CTGCAAGGCAAGGTCTACCGCGAACTGGAGGGGCGCCGCACCCTGCGCACCGAGGTCGACGGGCGCGGCTAC
TTCGTCAAGATCCACCGTGGCATCGGCTGGGGCGAGATCGCCAAGAACCTGCTCACCGCCAAGCTCCCGGTG
CTCGGCGCGCGCCAGGAGTGGCAGGCCATCCGGCGCCTGCACGAGGCCGGCGTAGCGACCATGACCGCGGTC
GCCTACGGCGAGCGCGGCAGCGATCCGGCGCGGCAGCATTCCTTCATCGTCACCGAGGAACTGGCGCCGACC
GTGGACCTCGAGGTGTTCTCCCAGGACTGGCGCGAACGTCCTCCACCGCCGCGGCTCAAGCGCGCGCTGGTC
GAGGCGGTGGCGCGGATGGTCGGCGACATGCACCGTGCCGGAGTCAACCATCGCGACTGCTACATCTGTCAT
TTCCTGTTGCACACCGACAAGCCGGTGAGCGCGGACGATTTCCGCCTCTCGGTGATCGACCTGCACCGTGCC
CAGACCCGCGACGCCACGCCGAAACGCTGGCGTAACAAGGATCTGGCGGCATTGTATTTCTCTGCGCTGGAC
ATCGGACTGACGCGTCGCGACAAGCTACGCTTCCTGCGCACCTATTTCCGCCGGCCGTTGCGCGAGATACTG
CGCGACGAGGCCGGCCTGCTGGCCTGGATGGAACGCAAGGCGGAAAAACTCTACGAACGCAAGCAGCGTTAC
GGAGACCTGCTCTGA
Translation:
MRLVLEEPFKRLWSGRDPFEAVEALQGKVYRELEGRRTLRTEVDGRGYFVKIHRGIGWGEIAKNLLTAKLPV
LGARQEWQAIRRLHEAGVATMTAVAYGERGSDPARQHSFIVTEELAPTVDLEVFSQDWRERPPPPRLKRALV
EAVARMVGDMHRAGVNHRDCYICHFLLHTDKPVSADDFRLSVIDLHRAQTRDATPKRWRNKDLAALYFSALD
IGLTRRDKLRFLRTYFRRPLREILRDEAGLLAWMERKAEKLYERKQRYGDLL*
AnnotationID:2888GeneID:2880
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 10:10:00
Gene Name:waaP
Confidence Code:1
GeneProduct:lipopolysaccharide kinase WaaP
Cell Localization:()
Synonyms:rfaP
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:lipopolysaccharide core biosynthesis protein
Functional Category Confidence Code:5
COGs:pfam06293
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58428.1| (AE016874) lipopolysaccharide core biosynthesis protein WaaP
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 268

 Score =  435 bits (1119), Expect = e-121
 Identities = 213/268 (79%), Positives = 229/268 (85%)

>gb|AAO58428.1| (AE016874) lipopolysaccharide core biosynthesis protein WaaP
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 268

 Score =  435 bits (1119), Expect = e-121
 Identities = 213/268 (79%), Positives = 229/268 (85%)

>gb|AAG58774.1|AE005590_9 (AE005590) putative LPS biosynthesis enzyme [Escherichia coli
           O157:H7 EDL933]
          Length = 268

 Score =  305 bits (780), Expect = 2e-82
 Identities = 146/259 (56%), Positives = 188/259 (72%)






 
Structural Features:
pfam06293, Kdo, Lipopolysaccharide kinase (Kdo/WaaP) family.
CD-Length = 206 residues, 100.0% aligned
Score =  197 bits (502), Expect = 1e-51
Identities = 78/214 (36%), Positives = 105/214 (49%), Gaps = 10/214 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5763




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22906




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35048




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2880_cdd.html


    		




 
URL:

















    ReferenceID:35873




  
Author/Investigator(s):
        Parker CT, Pradel E, Schnaitman CA.





  
Title:
        Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12.




  
PubMed:
        1732225


MedLine:
        




    
Source:
J Bacteriol. 1992 Feb;174(3):930-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1732225









           
Homologs By Global Alignment 

           

       



     
Gene ID:2880
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4674
                           PA5009_tr
                           translation of PA5009
                           268
                           99.62
                           99.99
                           0
                           1402.0
     

     
     
134687
                           gb|AAG08394.1|AE004913_9
                           (AE004913) lipopolysaccharide kinase WaaP [Pseudomonas aeruginosa PAO1]
                           268
                           99.62
                           99.99
                           0
                           1402.0
     

     
     
134688
                           gb|AAC33169.1|
                           (U63816) RfaP protein [Pseudomonas aeruginosa]
                           268
                           99.62
                           99.62
                           0
                           1401.0
     

     
     
134689
                           gb|AAD12056.1|
                           (AF087652) WaaP [Pseudomonas aeruginosa]
                           268
                           99.25
                           99.99
                           0
                           1398.0
     

     
     
134690
                           gb|AAN65975.1|AE016226_4
                           (AE016775) lipopolysaccharide core biosynthesis protein WaaP [Pseudomonas putida KT2440]
                           268
                           79.85
                           90.29
                           0
                           1156.0
     

     






      

          
GC ORFID: 45882How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5009Glimmer Score: 

          
Start: 5163751Stop: 5162945

          
Length: 807


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5009 translation of PA5009Homolog Bit Score 544.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3381 (ORF: BLASTX 434.0), t_PA5009 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGGCTGGTGCTGGAAGAGCCGTTCAAGCGCCTGTGGAGCGGGCGCGACCCGTTCGAGGCGGTGGAGGCG 
CTGCAAGGCAAGGTCTACCGCGAACTGGAGGGGCGCCGCACCCTGCGCACCGAGGTCGACGGGCGCGGCTAC 
TTCGTCAAGATCCACCGTGGCATCGGCTGGGGCGAGATCGCCAAGAACCTGCTCACCGCCAAGCTCCCGGTG 
CTCGGCGCGCGCCAGGAGTGGCAGGCCATCCGGCGCCTGCACGAGGCCGGCGTAGCGACCATGACCGCGGTC 
GCCTACGGCGAGCGCGGCAGCGATCCGGCGCGGCAGCATTCCTTCATCGTCACCGAGGAACTGGCGCCGACC 
GTGGACCTCGAGGTGTTCTCCCAGGACTGGCGCGAACGTCCTCCACCGCCGCGGCTCAAGCGCGCGCTGGTC 
GAGGCGGTGGCGCGGATGGTCGGCGACATGCACCGTGCCGGAGTCAACCATCGCGACTGCTACATCTGTCAT 
TTCCTGTTGCACACCGACAAGCCGGTGAGCGCGGACGATTTCCGCCTCTCGGTGATCGACCTGCACCGTGCC 
CAGACCCGCGACGCCACGCCGAAACGCTGGCGTAACAAGGATCTGGCGGCATTGTATTTCTCTGCGCTGGAC 
ATCGGACTGACGCGTCGCGACAAGCTACGCTTCCTGCGCACCTATTTCCGCCGGCCGTTGCGCGAGATACTG 
CGCGACGAGGCCGGCCTGCTGGCCTGGATGGAACGCAAGGCGGAAAAACTCTACGAACGCAAGCAGCGTTAC 
GGAGACCTGCTCTGA