ViewGene
Locus Name:
GeneID:
Location:

GeneID:2879
TrivialName: PA14_65450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65450
PAO1 Ortholog Locus:PA4954
Sequence Length:852
Protein Length:283
Start: 5831262
Stop: 5830411
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08339.1|AE004908_5 (AE004908) chemotaxis protein MotA [Pseudomonas aeruginosa PAO1]
Identities = 280/283 (98%), Positives = 282/283 (99%)
Sequence:
ATGTCAAAAATCATCGGCATCATCGTCGTTTTCGCCAGTGTCCTCGGGGGCTTCCTGCTCTCCGGCGGCAAG
ATCGGCGCCATCATCCAGCCCTTCGAGGTCCTGATCATCGGCGGTGCGGCACTGGGGGCGTTCCTCCAGGCC
AACCCGGGCAGCACGTTCATGGTGGTGCTGAAGAAAGCGCCGAAGATGTTCAGCAACCGCTTTACCCAGACC
TACTACCTCGAGGTGCTGGGCATGCTCTACGAGATCCTCAACAAGAGCCGGCGCGAAGGGATGATGGCGATC
GAGGCGGACATCGAGGACCCCGCGGCCAGTCCCATCTTCAGCAAGTACCCCGGCGTGCTGAAGGACGAACGG
ATGACCGCCTATGTCTGCGACTACCTGCGCATCATGTCCTCCGGCAACATGGCGCCGCACGAGCTGGAAGGC
CTGTTCGACATGGAGCTGTCCAGTCTCAAGGAGGACCTGGAGCATCCGTCCCACGCGGTGACCAAGGTCGCC
GACGCCCTGCCGGGCTTCGGTATCGTCGCCGCGGTACTCGGCATCGTCATCACCATGGCGTTGCTTGGCGAA
GGCAGCCAGGCGGAGATCGGCCACCACGTCGCCGCCGCCCTGGTGGGTACCTTCCTCGGGATTCTCGCCGCC
TACGGCTTCGTCGGCCCGCTGGCCGTCGCGCTGGAGCACGATGCCAAGGAAGAGTTGAACATGTTCGAAGCG
ATCAAGGCCTGCCTGGTCGCTTCTGCTTCCGGCATGCCGCCTTCGCTGGCCGTCGAGTTCGGCCGCAAGGTG
CTGCTGCCCGCCCATCGGCCGACCTTCGCCGAGCTCGAGCAGGCCGTGCGTGGCCGCTGA
Translation:
MSKIIGIIVVFASVLGGFLLSGGKIGAIIQPFEVLIIGGAALGAFLQANPGSTFMVVLKKAPKMFSNRFTQT
YYLEVLGMLYEILNKSRREGMMAIEADIEDPAASPIFSKYPGVLKDERMTAYVCDYLRIMSSGNMAPHELEG
LFDMELSSLKEDLEHPSHAVTKVADALPGFGIVAAVLGIVITMALLGEGSQAEIGHHVAAALVGTFLGILAA
YGFVGPLAVALEHDAKEELNMFEAIKACLVASASGMPPSLAVEFGRKVLLPAHRPTFAELEQAVRGR*
AnnotationID:2887GeneID:2879
AnnotatorUID: danlee
Modification Date/Time: 2006-01-31 14:02:54
Gene Name:motA
Confidence Code:1
GeneProduct:chemotaxis protein MotA
Cell Localization:()
Synonyms:rpmA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:flagellar motor component
Functional Category Confidence Code:5
COGs:COG1291
Secondary Functional Category(ies):Motility & Attachment
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58381.1| (AE016873) chemotaxis motA protein [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 283

 Score =  454 bits (1168), Expect = e-127
 Identities = 228/283 (80%), Positives = 252/283 (89%)

>gb|AAN70472.1|AE016688_11 (AE016792) motility protein, MotA family [Pseudomonas putida
           KT2440]
          Length = 283

 Score =  454 bits (1168), Expect = e-127
 Identities = 230/283 (81%), Positives = 254/283 (89%)

>emb|CAD18562.1| (AL646084) PROBABLE CHEMOTAXIS (MOTILITY PROTEIN A) TRANSMEMBRANE
           [Ralstonia solanacearum]
          Length = 286

 Score =  278 bits (712), Expect = 2e-74
 Identities = 140/279 (50%), Positives = 190/279 (68%), Gaps = 2/279 (0%)






 
Structural Features:
COG1291, MotA, Flagellar motor component [Cell motility and secretion]
CD-Length = 266 residues,  98.5% aligned
Score =  260 bits (666), Expect = 1e-70
Identities = 113/283 (39%), Positives = 164/283 (57%), Gaps = 21/283 (7%)






   
Genomic Context:






   
Comment:
wrt naming of motAB and motCD genes, PMID: 15629949 (Toutain, Zegans and O’Toole, 2005) agrees with 
PAO1 and PA14 annotations, but disagrees with PMID 15375113 (Doyle, Hawkins and McCarter, 2004).



















    ReferenceID:5761




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22904




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35101




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2879_cdd.html


    		




 
URL:

















    ReferenceID:35894




  
Author/Investigator(s):
        Kato J, Nakamura T, Kuroda A, Ohtake H.





  
Title:
        Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa.




  
PubMed:
        10052136


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1999 Jan;63(1):155-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10052136

















    ReferenceID:35895




  
Author/Investigator(s):
        Stamm LV, Bergen HL.





  
Title:
        Molecular characterization of a flagellar (fla) operon in the oral spirochete Treponema denticola ATCC 35405.




  
PubMed:
        10481082


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 Oct 1;179(1):31-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10481082

















    ReferenceID:38302




  
Author/Investigator(s):
        Doyle TB, Hawkins AC, McCarter LL.





  
Title:
        The complex flagellar torque generator of Pseudomonas aeruginosa.




  
PubMed:
        15375113


MedLine:
        




    
Source:
J Bacteriol. 2004 Oct;186(19):6341-50.






    
Reference Type: 







    
Data:


    		




 
URL:

















    ReferenceID:38305




  
Author/Investigator(s):
        Toutain CM, Zegans ME, O'Toole GA.





  
Title:
        Evidence for two flagellar stators and their role in the motility of Pseudomonas aeruginosa.




  
PubMed:
        15629949


MedLine:
        




    
Source:
J Bacteriol. 2005 Jan;187(2):771-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2879
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4673
                           PA4954_tr
                           translation of PA4954
                           283
                           98.93
                           99.64
                           0
                           1401.0
     

     
     
134652
                           gb|AAG08339.1|AE004908_5
                           (AE004908) chemotaxis protein MotA [Pseudomonas aeruginosa PAO1]
                           283
                           98.93
                           99.64
                           0
                           1401.0
     

     
     
134653
                           gb|AAD33690.1|AF136403_2
                           (AF136403) RpmA [Pseudomonas aeruginosa]
                           285
                           91.22
                           92.63
                           4
                           1270.0
     

     
     
134655
                           gb|AAN70472.1|AE016688_11
                           (AE016792) motility protein, MotA family [Pseudomonas putida KT2440]
                           283
                           81.27
                           89.75
                           0
                           1168.0
     

     
     
134654
                           gb|AAO58381.1|
                           (AE016873) chemotaxis motA protein [Pseudomonas syringae pv. tomato str. DC3000]
                           283
                           80.56
                           89.04
                           0
                           1168.0
     

     






      

          
GC ORFID: 45881How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4954Glimmer Score: 

          
Start: 5094943Stop: 5094092

          
Length: 852


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4954 translation of PA4954Homolog Bit Score 544.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3419 (ORF: BLASTX 453.0), t_PA4954 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCAAAAATCATCGGCATCATCGTCGTTTTCGCCAGTGTCCTCGGGGGCTTCCTGCTCTCCGGCGGCAAG 
ATCGGCGCCATCATCCAGCCCTTCGAGGTCCTGATCATCGGCGGTGCGGCACTGGGGGCGTTCCTCCAGGCC 
AACCCGGGCAGCACGTTCATGGTGGTGCTGAAGAAAGCGCCGAAGATGTTCAGCAACCGCTTTACCCAGACC 
TACTACCTCGAGGTGCTGGGCATGCTCTACGAGATCCTCAACAAGAGCCGGCGCGAAGGGATGATGGCGATC 
GAGGCGGACATCGAGGACCCCGCGGCCAGTCCCATCTTCAGCAAGTACCCCGGCGTGCTGAAGGACGAACGG 
ATGACCGCCTATGTCTGCGACTACCTGCGCATCATGTCCTCCGGCAACATGGCGCCGCACGAGCTGGAAGGC 
CTGTTCGACATGGAGCTGTCCAGTCTCAAGGAGGACCTGGAGCATCCGTCCCACGCGGTGACCAAGGTCGCC 
GACGCCCTGCCGGGCTTCGGTATCGTCGCCGCGGTACTCGGCATCGTCATCACCATGGCGTTGCTTGGCGAA 
GGCAGCCAGGCGGAGATCGGCCACCACGTCGCCGCCGCCCTGGTGGGTACCTTCCTCGGGATTCTCGCCGCC 
TACGGCTTCGTCGGCCCGCTGGCCGTCGCGCTGGAGCACGATGCCAAGGAAGAGTTGAACATGTTCGAAGCG 
ATCAAGGCCTGCCTGGTCGCTTCTGCTTCCGGCATGCCGCCTTCGCTGGCCGTCGAGTTCGGCCGCAAGGTG 
CTGCTGCCCGCCCATCGGCCGACCTTCGCCGAGCTCGAGCAGGCCGTGCGTGGCCGCTGA