ViewGene
Locus Name:
GeneID:
Location:

GeneID:2878
TrivialName: PA14_17070
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17070
PAO1 Ortholog Locus:PA3655
Sequence Length:870
Protein Length:289
Start: 1463983
Stop: 1464852
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07043.1|AE004785_7 (AE004785) elongation factor Ts [Pseudomonas aeruginosa PAO1]
Identities = 289/289 (100%), Positives = 289/289 (100%)
Sequence:
ATGGCAGAAATTACTGCAGCCATGGTTAAAGAACTGCGCGAGCGTACCGGCTTGGGCATGATGGAGTGCAAG
AAGGCGCTGACCGCCGCTGGCGGCGACATCGAGAAGGCCATCGACGACATGCGCGCTGCCGGTGCGATCAAG
GCTGCCAAGAAGGCCGGCAACATTGCCGCCGAAGGCTCGATCGCCGTCAAGATCGCTGCCGACAACAAGGCC
GCCGTGATCATCGAAGTCAACTCCCAGACCGACTTCCTGGCCCTCCAGGACGACTTCAAGGGCTTCGTCGCC
GAGAGCCTGGAAAAGGCCTTCAACGAGAAGCTGACCGACGCCGCTCCGCTGGTCGAAGCGCGTGAGGAGGCT
CGTCTGGCCCTGGTCGCCAAGACCGGCGAGAACGTCAACATCCGCCGCCTGACCCGCGTCGAGGGCGACGTG
GTCGGCGCCTACCTGCACGGCCACCGCATCGGTGTCGTGGTCAACCTGAAAGGCGGCAACCCGGAACTGGCC
AAGGACATCGCCATGCATGTCGCCGCCAGCAACCCGCAGTTCCTGAGCGCTTCGGAAGTTTCCGAAGAAGCG
ATCGCGAAAGAGAAGGAAATCTTCCTGGCGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAGAACATCGTC
GAGAACATGGTCAAGGGCCGCATCAGCAAGTTCCTCGCCGAAGCGAGCCTGGTCGAGCAGCCTTTCGTGAAG
AACCCGGAAGTGAAGGTCGGCGATCTGGCCAAGCAGGCCGGCGCCGAAATCGTTTCCTTCGTTCGTTACGAA
GTGGGCGAAGGCATCGAGAAGGCCGAAGTGGACTTCGCTGCCGAAGTTGCCGCTCAAGTAGCCGCCACCAAG
CAGTAA
Translation:
MAEITAAMVKELRERTGLGMMECKKALTAAGGDIEKAIDDMRAAGAIKAAKKAGNIAAEGSIAVKIAADNKA
AVIIEVNSQTDFLALQDDFKGFVAESLEKAFNEKLTDAAPLVEAREEARLALVAKTGENVNIRRLTRVEGDV
VGAYLHGHRIGVVVNLKGGNPELAKDIAMHVAASNPQFLSASEVSEEAIAKEKEIFLALNADKIAGKPENIV
ENMVKGRISKFLAEASLVEQPFVKNPEVKVGDLAKQAGAEIVSFVRYEVGEGIEKAEVDFAAEVAAQVAATK
Q*
AnnotationID:2886GeneID:2878
AnnotatorUID: diggins
Modification Date/Time: 2005-04-21 05:05:57
Gene Name:tsf
Confidence Code:2
GeneProduct:translation elongation factor Ts
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0264,pfam00889
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55055.1| (AE016861) translation elongation factor Ts [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 287

 Score =  431 bits (1107), Expect = e-120
 Identities = 228/289 (78%), Positives = 252/289 (87%), Gaps = 2/289 (0%)

>gb|AAN78700.1|AE016755_200 (AE016755) Elongation factor Ts [Escherichia coli CFT073]
          Length = 283

 Score =  301 bits (770), Expect = 4e-81
 Identities = 161/282 (57%), Positives = 199/282 (70%), Gaps = 4/282 (1%)






 
Structural Features:
COG0264, Tsf, Translation elongation factor Ts 
[Translation, ribosomal structure and biogenesis]
CD-Length = 296 residues, 100.0% aligned
Score =  299 bits (766), Expect = 7e-82
Identities = 168/298 (56%), Positives = 209/298 (70%), Gaps = 14/298 (4%)

pfam00889, EF_TS, Elongation factor TS.
CD-Length = 220 residues, 100.0% aligned
Score =  185 bits (472), Expect = 8e-48
Identities = 108/224 (48%), Positives = 145/224 (64%), Gaps = 17/224 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5759




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22902




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31888




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2878_cdd.html


    		




 
URL:

















    ReferenceID:34224




  
Author/Investigator(s):
        Ohnishi M, Janosi L, Shuda M, Matsumoto H, Hayashi T, Terawaki Y, Kaji A.





  
Title:
        Molecular cloning, sequencing, purification, and characterization of Pseudomonas aeruginosa ribosome recycling factor.




  
PubMed:
        9973356


MedLine:
        




    
Source:
J Bacteriol. 1999 Feb;181(4):1281-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9973356

















    ReferenceID:34225




  
Author/Investigator(s):
        Martinez G, Harel J, Lacouture S, Gottschalk M.





  
Title:
        Cloning and partial characterization of the gene encoding the putative elongation factor Ts of Streptococcus suis serotype 2.




  
PubMed:
        12955311


MedLine:
        




    
Source:
Arch Microbiol. 2003 Oct;180(4):298-302. Epub 2003 Sep 02.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12955311

















    ReferenceID:34226




  
Author/Investigator(s):
        Le Dantec L, Bove JM, Saillard C.





  
Title:
        Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon.




  
PubMed:
        9732535


MedLine:
        




    
Source:
Curr Microbiol. 1998 Oct;37(4):269-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9732535

















    ReferenceID:34227




  
Author/Investigator(s):
        Chevalier C, Saillard C, Bove JM.





  
Title:
        Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein.




  
PubMed:
        2139649


MedLine:
        




    
Source:
J Bacteriol. 1990 May;172(5):2693-703.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2139649

















    ReferenceID:34228




  
Author/Investigator(s):
        An G, Bendiak DS, Mamelak LA, Friesen JD.





  
Title:
        Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.




  
PubMed:
        6272196


MedLine:
        




    
Source:
Nucleic Acids Res. 1981 Aug 25;9(16):4163-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6272196









           
Homologs By Global Alignment 

           

       



     
Gene ID:2878
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4672
                           PA3655_tr
                           translation of PA3655
                           289
                           99.99
                           99.99
                           0
                           1402.0
     

     
     
134566
                           gb|AAG07043.1|AE004785_7
                           (AE004785) elongation factor Ts [Pseudomonas aeruginosa PAO1]
                           289
                           99.99
                           99.99
                           0
                           1402.0
     

     
     
134567
                           dbj|BAA32343.1|
                           (AB010087) elongation factor Ts [Pseudomonas aeruginosa]
                           289
                           99.99
                           99.99
                           0
                           1402.0
     

     
     
134568
                           gb|AAO55055.1|
                           (AE016861) translation elongation factor Ts [Pseudomonas syringae pv. tomato str. DC3000]
                           289
                           78.89
                           87.19
                           2
                           1109.0
     

     
     
134569
                           gb|AAN67213.1|AE016348_5
                           (AE016779) translation elongation factor Ts [Pseudomonas putida KT2440]
                           289
                           78.20
                           87.88
                           2
                           1103.0
     

     






      

          
GC ORFID: 45880How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3655Glimmer Score: 

          
Start: 3489954Stop: 3489085

          
Length: 870


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3655 translation of PA3655Homolog Bit Score 544.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4620 (ORF: BLASTX 427.0), t_PA3655 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCAGAAATTACTGCAGCCATGGTTAAAGAACTGCGCGAGCGTACCGGCTTGGGCATGATGGAGTGCAAG 
AAGGCGCTGACCGCCGCTGGCGGCGACATCGAGAAGGCCATCGACGACATGCGCGCTGCCGGTGCGATCAAG 
GCTGCCAAGAAGGCCGGCAACATTGCCGCCGAAGGCTCGATCGCCGTCAAGATCGCTGCCGACAACAAGGCC 
GCCGTGATCATCGAAGTCAACTCCCAGACCGACTTCCTGGCCCTCCAGGACGACTTCAAGGGCTTCGTCGCC 
GAGAGCCTGGAAAAGGCCTTCAACGAGAAGCTGACCGACGCCGCTCCGCTGGTCGAAGCGCGTGAGGAGGCT 
CGTCTGGCCCTGGTCGCCAAGACCGGCGAGAACGTCAACATCCGCCGCCTGACCCGCGTCGAGGGCGACGTG 
GTCGGCGCCTACCTGCACGGCCACCGCATCGGTGTCGTGGTCAACCTGAAAGGCGGCAACCCGGAACTGGCC 
AAGGACATCGCCATGCATGTCGCCGCCAGCAACCCGCAGTTCCTGAGCGCTTCGGAAGTTTCCGAAGAAGCG 
ATCGCGAAAGAGAAGGAAATCTTCCTGGCGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAGAACATCGTC 
GAGAACATGGTCAAGGGCCGCATCAGCAAGTTCCTCGCCGAAGCGAGCCTGGTCGAGCAGCCTTTCGTGAAG 
AACCCGGAAGTGAAGGTCGGCGATCTGGCCAAGCAGGCCGGCGCCGAAATCGTTTCCTTCGTTCGTTACGAA 
GTGGGCGAAGGCATCGAGAAGGCCGAAGTGGACTTCGCTGCCGAAGTTGCCGCTCAAGTAGCCGCCACCAAG 
CAGTAA