ViewGene
Locus Name:
GeneID:
Location:

GeneID:2871
TrivialName: PA14_58700
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58700
PAO1 Ortholog Locus:PA4524
Sequence Length:849
Protein Length:282
Start: 5231738
Stop: 5232586
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07912.1|AE004866_6 (AE004866) nicotinate-nucleotide pyrophosphorylase [Pseudomonas aeruginosa PAO1]
Identities = 280/282 (99%), Positives = 280/282 (99%)
Sequence:
ATGCCTAACCTCACCCTTGCCGACCTCCAAGGCGAAATCCAGGCCAACGTCCGCACCGCCCTGGCGGAAGAC
GTCGGCGGCGGCGACCTTACCGCTCAGTTGATCGATCCACAGCGCGAGGCCGAGGCCCGGGTGATTACCCGC
GAGCACGCGACGATCGCGGGCCGCGCCTGGGTCGACGAGGTCTTCCGCCAGGTCGATCCCCGAGTGCTGGTG
ACCTGGCAGGTGGAGGACGGCCAGCGAGTCGAGCCGAACCAGATGCTGTTCCAGCTAAAAGGTCCGGCCCGC
GCCCTGCTCACCGGCGAGCGCAGCGCGCTGAACTTCCTCCAGCTGCTTTCCGGTACCGCCACCCGCAGCCAG
CACTACGCCGACCTGGTCGCAGGCACCACGGTGAAGCTGCTCGACACCCGCAAGACCCTGCCCGGCCTGCGC
CTCGCGCAGAAGTACGCGGTCACCTGCGGCGGTTGCCACAACCACCGCATCGGCCTCTACGATGCCTTCCTG
ATCAAGGAAAACCACATCGCCGCCTGCGGCGGTATCCATCGGGCAATCGCCGAAGCCCGGCGCATAGCACCC
GGCAAGCCGGTGGAAGTGGAAGTGGAGAACCTCGATGAACTGCGCCAGGCGCTGGAGGCCGGCGCCGATATC
GTCATGCTCGACGAACTCAGCCTGGACGACATGCGCACCGCCGTCGCCCTCACCGCCGGCCGTGCCAAGCTG
GAAGCTTCCGGCGGAATCAACGAGGGCACCCTGCGCAACATCGCCGAGACCGGCGTCGACTACATCTCCATC
GGCACCCTGACCAAGGATGTCAGGGCCGTCGACCTGTCCATGCGCCTCACCCTCTGA
Translation:
MPNLTLADLQGEIQANVRTALAEDVGGGDLTAQLIDPQREAEARVITREHATIAGRAWVDEVFRQVDPRVLV
TWQVEDGQRVEPNQMLFQLKGPARALLTGERSALNFLQLLSGTATRSQHYADLVAGTTVKLLDTRKTLPGLR
LAQKYAVTCGGCHNHRIGLYDAFLIKENHIAACGGIHRAIAEARRIAPGKPVEVEVENLDELRQALEAGADI
VMLDELSLDDMRTAVALTAGRAKLEASGGINEGTLRNIAETGVDYISIGTLTKDVRAVDLSMRLTL*
AnnotationID:2879GeneID:2871
AnnotatorUID: diggins
Modification Date/Time: 2005-05-04 15:03:29
Gene Name:nadC
Confidence Code:2
GeneProduct:nicotinate-nucleotide pyrophosphorylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:quinolinate phosphoribosyltransferase
Functional Category Confidence Code:5
COGs:COG0157,pfam01729,KOG3008,pfam02749
Secondary Functional Category(ies):
EC Number:2.4.2.19
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66412.1|AE016268_11 (AE016776) nicotinate-nucleotide pyrophosphorylase [Pseudomonas
           putida KT2440]
          Length = 282

 Score =  425 bits (1092), Expect = e-118
 Identities = 215/282 (76%), Positives = 244/282 (86%)

>gb|AAL32048.1|AF439854_1 (AF439854) NadC [Vibrio vulnificus]
          Length = 295

 Score =  313 bits (801), Expect = 9e-85
 Identities = 160/273 (58%), Positives = 197/273 (72%), Gaps = 5/273 (1%)






 
Structural Features:
COG0157, NadC, Nicotinate-nucleotide pyrophosphorylase [Coenzyme metabolism]
CD-Length = 280 residues,  97.5% aligned
Score =  337 bits (866), Expect = 2e-93
Identities = 142/273 (52%), Positives = 184/273 (67%), Gaps = 4/273 (1%)

pfam01729, QRPTase_C, Quinolinate phosphoribosyl transferase
CD-Length = 168 residues,  99.4% aligned
Score =  216 bits (552), Expect = 4e-57
Identities = 89/167 (53%), Positives = 112/167 (67%), Gaps = 3/167 (1%)

KOG3008, Quinolinate phosphoribosyl transferase 
[Nucleotide transport and metabolism]
CD-Length = 300 residues,  88.0% aligned
Score =  178 bits (452), Expect = 1e-45
Identities = 91/267 (34%), Positives = 138/267 (51%), Gaps = 10/267 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5745




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22888




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31570




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2871_cdd.html


    		




 
URL:

















    ReferenceID:32965




  
Author/Investigator(s):
        Bhatia R, Calvo KC.





  
Title:
        The sequencing expression, purification, and steady-state kinetic analysis of quinolinate phosphoribosyl transferase from Escherichia coli.




  
PubMed:
        8561507


MedLine:
        




    
Source:
Arch Biochem Biophys. 1996 Jan 15;325(2):270-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8561507

















    ReferenceID:32966




  
Author/Investigator(s):
        Whitchurch CB, Mattick JS.





  
Title:
        Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria.




  
PubMed:
        7959070


MedLine:
        




    
Source:
Gene. 1994 Dec 2;150(1):9-15.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7959070

















    ReferenceID:32967




  
Author/Investigator(s):
        Hughes KT, Roth JR, Olivera BM.





  
Title:
        A genetic characterization of the nadC gene of Salmonella typhimurium.




  
PubMed:
        2029967


MedLine:
        




    
Source:
Genetics. 1991 Apr;127(4):657-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2029967

















    ReferenceID:32968




  
Author/Investigator(s):
        Roberts RE, Lienhard CI, Gaines CG, Smith JM, Guest JR.





  
Title:
        Genetic and molecular characterization of the guaC-nadC-aroP region of Escherichia coli K-12.




  
PubMed:
        3275629


MedLine:
        




    
Source:
J Bacteriol. 1988 Jan;170(1):463-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3275629

















    ReferenceID:32969




  
Author/Investigator(s):
        Holley EA, Spector MP, Foster JW.





  
Title:
        Regulation of NAD biosynthesis in Salmonella typhimurium: expression of nad-lac gene fusions and identification of a nad regulatory locus.




  
PubMed:
        3934331


MedLine:
        




    
Source:
J Gen Microbiol. 1985 Oct;131 ( Pt 10):2759-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3934331

















    ReferenceID:32970




  
Author/Investigator(s):
        Foster JW, Moat AG.





  
Title:
        Mapping and characterization of the nad genes in Salmonella typhimurium LT-2.




  
PubMed:
        203571


MedLine:
        




    
Source:
J Bacteriol. 1978 Feb;133(2):775-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=203571

















    ReferenceID:32971




  
Author/Investigator(s):
        Holley EA, Foster JW.





  
Title:
        Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions.




  
PubMed:
        6752128


MedLine:
        




    
Source:
J Bacteriol. 1982 Nov;152(2):959-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6752128

















    ReferenceID:32972




  
Author/Investigator(s):
        Griffith GR, Chandler JL, Gholson RK.





  
Title:
        Studies on the de novo biosynthesis of NAD in Escherichia coli. The separation of the nadB gene product from the nadA gene product and its purification.




  
PubMed:
        238844


MedLine:
        




    
Source:
Eur J Biochem. 1975 May;54(1):239-45.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=238844









           
Homologs By Global Alignment 

           

       



     
Gene ID:2871
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4664
                           PA4524_tr
                           translation of PA4524
                           282
                           99.29
                           99.29
                           0
                           1403.0
     

     
     
134315
                           gb|AAG07912.1|AE004866_6
                           (AE004866) nicotinate-nucleotide pyrophosphorylase [Pseudomonas aeruginosa PAO1]
                           282
                           99.29
                           99.29
                           0
                           1403.0
     

     
     
134316
                           gb|AAC99834.1|
                           (U27709) nicotinate-nucleotide pyrophosphorylase [Pseudomonas aeruginosa]
                           286
                           91.95
                           92.65
                           7
                           1274.0
     

     
     
134317
                           gb|AAO54482.1|
                           (AE016859) nicotinate-nucleotide pyrophosphorylase [Pseudomonas syringae pv. tomato str. DC3000]
                           282
                           77.30
                           88.29
                           0
                           1114.0
     

     
     
134318
                           gb|AAN66412.1|AE016268_11
                           (AE016776) nicotinate-nucleotide pyrophosphorylase [Pseudomonas putida KT2440]
                           282
                           76.24
                           86.52
                           0
                           1092.0
     

     






      

          
GC ORFID: 45873How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4524Glimmer Score: 

          
Start: 4495417Stop: 4496265

          
Length: 849


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4524 translation of PA4524Homolog Bit Score 545.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2529 (ORF: BLASTX 438.0), t_PA4524 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCTAACCTCACCCTTGCCGACCTCCAAGGCGAAATCCAGGCCAACGTCCGCACCGCCCTGGCGGAAGAC 
GTCGGCGGCGGCGACCTTACCGCTCAGTTGATCGATCCACAGCGCGAGGCCGAGGCCCGGGTGATTACCCGC 
GAGCACGCGACGATCGCGGGCCGCGCCTGGGTCGACGAGGTCTTCCGCCAGGTCGATCCCCGAGTGCTGGTG 
ACCTGGCAGGTGGAGGACGGCCAGCGAGTCGAGCCGAACCAGATGCTGTTCCAGCTAAAAGGTCCGGCCCGC 
GCCCTGCTCACCGGCGAGCGCAGCGCGCTGAACTTCCTCCAGCTGCTTTCCGGTACCGCCACCCGCAGCCAG 
CACTACGCCGACCTGGTCGCAGGCACCACGGTGAAGCTGCTCGACACCCGCAAGACCCTGCCCGGCCTGCGC 
CTCGCGCAGAAGTACGCGGTCACCTGCGGCGGTTGCCACAACCACCGCATCGGCCTCTACGATGCCTTCCTG 
ATCAAGGAAAACCACATCGCCGCCTGCGGCGGTATCCATCGGGCAATCGCCGAAGCCCGGCGCATAGCACCC 
GGCAAGCCGGTGGAAGTGGAAGTGGAGAACCTCGATGAACTGCGCCAGGCGCTGGAGGCCGGCGCCGATATC 
GTCATGCTCGACGAACTCAGCCTGGACGACATGCGCACCGCCGTCGCCCTCACCGCCGGCCGTGCCAAGCTG 
GAAGCTTCCGGCGGAATCAACGAGGGCACCCTGCGCAACATCGCCGAGACCGGCGTCGACTACATCTCCATC 
GGCACCCTGACCAAGGATGTCAGGGCCGTCGACCTGTCCATGCGCCTCACCCTCTGA