ViewGene
Locus Name:
GeneID:
Location:

GeneID:2869
TrivialName: PA14_40450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_40450
PAO1 Ortholog Locus:PA1858
Sequence Length:822
Protein Length:273
Start: 3614373
Stop: 3615194
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05247.1|AE004612_1 (AE004612) streptomycin 3''-phosphotransferase [Pseudomonas aeruginosa PAO1]
Identities = 264/271 (97%), Positives = 270/271 (99%)
Sequence:
GTGAACGAATCCCTCGACTTCTCGCCCTACCTCGAGCGCTGGGCGTTGCAACCCGACGGCGAGCCCTTCGCC
ACCCACAGCAGCCGCCTGCTGCCGGTGCGCCATCGCGGCGCAGCGGCGATGCTGAAGATATCCAGTGCCGAG
GAAGAGCGTTTCGGCCACGTACTGCTCAACTGGTGGGACGGCCAGGGCGCGGCCAGGGTGCTGGCCTACGAC
CACCAGGCGCTGCTGATGGAGCGTGCCACCGGCGGGCGCTCGCTGCTGGAGATGGTCAAGCGCGGCGACGAC
GACGAGGCTACGCGTATCCTCTGCCAGGCCATCGAACGCATCCATGCCCCGCGCCCGGGTCCGTTGCCGGAA
CTGACCCCGCTGGAGCGCTGGTTCGACTCGCTGTATGCCGCCGAGCGCCGCTACGGCGGGCTCTACGTCGAC
TGCGCGAACGCCGCCCGCTACCTGCTGGAGACCGCTCGCGAACAGCGCCCGTTGCATGGCGACATCCACCAC
GGCAATGTGCTCGACTTCGGCGAGCGCGGCTGGCAAGTCATCGACCCCAAGCGGCTCTACGGCGAGCGCGGC
TACGACTACGCCAACCTGTTCACCAATCCCGACGCGAAGACCGCCCAGGCGCCCGGCCGTTTCGAGCGCCAG
CTGGAGATCGTCGTCGCCGCCAGCGGCCTGCAACGCCGCCGCCTGCTGCTGTGGATCCTGGCCTGGACCGGA
CTCTCGGCGGCCTGGTTCGACGAGGACGGCAGCCGCGAGGAGACCGACGCCGAGCTGGCCATCGCCCGCCTG
GCCCTCGCCAGCCTGTCTCGCGAAGGCTGA
Translation:
MNESLDFSPYLERWALQPDGEPFATHSSRLLPVRHRGAAAMLKISSAEEERFGHVLLNWWDGQGAARVLAYD
HQALLMERATGGRSLLEMVKRGDDDEATRILCQAIERIHAPRPGPLPELTPLERWFDSLYAAERRYGGLYVD
CANAARYLLETAREQRPLHGDIHHGNVLDFGERGWQVIDPKRLYGERGYDYANLFTNPDAKTAQAPGRFERQ
LEIVVAASGLQRRRLLLWILAWTGLSAAWFDEDGSREETDAELAIARLALASLSREG*
AnnotationID:2877GeneID:2869
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 14:02:57
Gene Name:str
Confidence Code:2
GeneProduct:streptomycin 3''-phosphotransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:streptomycin-3-kinase
Functional Category Confidence Code:5
COGs:COG3570,pfam04655
Secondary Functional Category(ies):defense mechanisms
EC Number:2.7.1.87
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN18030.1| (AY135187) putative streptomycin-3-kinase [Pseudomonas aurantiaca]
          Length = 272

 Score =  289 bits (740), Expect = 1e-77
 Identities = 144/267 (53%), Positives = 180/267 (67%)

>emb|CAC46142.1| (AL591787) PUTATIVE ANTIBIOTIC RESISTANCE (KINASE) PROTEIN
           [Sinorhizobium meliloti]
          Length = 264

 Score =  278 bits (711), Expect = 2e-74
 Identities = 138/263 (52%), Positives = 176/263 (66%), Gaps = 2/263 (0%)

>gb|AAF10031.1|AE001904_7 (AE001904) streptomycin 3-kinase [Deinococcus radiodurans]
          Length = 283

 Score =  270 bits (689), Expect = 9e-72
 Identities = 146/272 (53%), Positives = 176/272 (64%), Gaps = 12/272 (4%)






 
Structural Features:
COG3570, StrB, Streptomycin 6-kinase [Defense mechanisms]
CD-Length = 274 residues,  99.6% aligned
Score =  382 bits (981), Expect = 3e-107
Identities = 166/273 (60%), Positives = 194/273 (71%), Gaps = 4/273 (1%)

pfam04655, APH_6_hur, Aminoglycoside/hydroxyurea antibiotic resistance kinase.
CD-Length = 253 residues, 100.0% aligned
Score =  271 bits (695), Expect = 5e-74
Identities = 140/253 (55%), Positives = 161/253 (63%), Gaps = 15/253 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14685




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22884




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37087




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2869_cdd.html


    		




 
URL:

















    ReferenceID:37784




  
Author/Investigator(s):
        Mazodier P, Cossart P, Giraud E, Gasser F.





  
Title:
        Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.




  
PubMed:
        3889831


MedLine:
        




    
Source:
Nucleic Acids Res. 1985 Jan 11;13(1):195-205.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3889831









           
Homologs By Global Alignment 

           

       



     
Gene ID:2869
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4662
                           PA1858_tr
                           translation of PA1858
                           275
                           96.00
                           98.18
                           2
                           1401.0
     

     
     
134307
                           gb|AAG05247.1|AE004612_1
                           (AE004612) streptomycin 3''-phosphotransferase [Pseudomonas aeruginosa PAO1]
                           275
                           95.63
                           98.18
                           2
                           1398.0
     

     
     
134308
                           gb|AAN18030.1|
                           (AY135187) putative streptomycin-3-kinase [Pseudomonas aurantiaca]
                           277
                           51.98
                           64.98
                           9
                           737.0
     

     
     
134310
                           gb|AAF10031.1|AE001904_7
                           (AE001904) streptomycin 3-kinase [Deinococcus radiodurans] [Deinococcus radiodurans R1]
                           288
                           50.69
                           61.45
                           20
                           690.0
     

     
     
134309
                           emb|CAC46142.1|
                           (AL591787) PUTATIVE ANTIBIOTIC RESISTANCE (KINASE) PROTEIN [Sinorhizobium meliloti]
                           273
                           50.54
                           64.46
                           9
                           707.0
     

     






      

          
GC ORFID: 45871How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1858Glimmer Score: 

          
Start: 1339537Stop: 1338716

          
Length: 822


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1858 translation of PA1858Homolog Bit Score 545.0

          
Other Homologs:  t_PA1858 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAACGAATCCCTCGACTTCTCGCCCTACCTCGAGCGCTGGGCGTTGCAACCCGACGGCGAGCCCTTCGCC 
ACCCACAGCAGCCGCCTGCTGCCGGTGCGCCATCGCGGCGCAGCGGCGATGCTGAAGATATCCAGTGCCGAG 
GAAGAGCGTTTCGGCCACGTACTGCTCAACTGGTGGGACGGCCAGGGCGCGGCCAGGGTGCTGGCCTACGAC 
CACCAGGCGCTGCTGATGGAGCGTGCCACCGGCGGGCGCTCGCTGCTGGAGATGGTCAAGCGCGGCGACGAC 
GACGAGGCTACGCGTATCCTCTGCCAGGCCATCGAACGCATCCATGCCCCGCGCCCGGGTCCGTTGCCGGAA 
CTGACCCCGCTGGAGCGCTGGTTCGACTCGCTGTATGCCGCCGAGCGCCGCTACGGCGGGCTCTACGTCGAC 
TGCGCGAACGCCGCCCGCTACCTGCTGGAGACCGCTCGCGAACAGCGCCCGTTGCATGGCGACATCCACCAC 
GGCAATGTGCTCGACTTCGGCGAGCGCGGCTGGCAAGTCATCGACCCCAAGCGGCTCTACGGCGAGCGCGGC 
TACGACTACGCCAACCTGTTCACCAATCCCGACGCGAAGACCGCCCAGGCGCCCGGCCGTTTCGAGCGCCAG 
CTGGAGATCGTCGTCGCCGCCAGCGGCCTGCAACGCCGCCGCCTGCTGCTGTGGATCCTGGCCTGGACCGGA 
CTCTCGGCGGCCTGGTTCGACGAGGACGGCAGCCGCGAGGAGACCGACGCCGAGCTGGCCATCGCCCGCCTG 
GCCCTCGCCAGCCTGTCTCGCGAAGGCTGA