ViewGene
Locus Name:
GeneID:
Location:

GeneID:286
TrivialName: PA14_08820
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_08820
PAO1 Ortholog Locus:PA4266
Sequence Length:2121
Protein Length:706
Start: 755665
Stop: 757785
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07654.1|AE004842_3 (AE004842) elongation factor G [Pseudomonas aeruginosa PAO1]
Identities = 706/706 (100%), Positives = 706/706 (100%)
Sequence:
GTGGCCCGTACTACACCCATCAACCGCTACCGTAATATCGGTATCTGTGCCCACGTTGACGCGGGCAAGACC
ACCACCACGGAGCGGGTTCTGTTCTATACCGGCGTCAACCACAAGCTGGGCGAAGTGCATGACGGCGCTGCG
ACCACCGACTGGATGGTGCAGGAGCAGGAGCGCGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGG
AAGGGCTCGCGTGGTCAGTACGACAACTATCGCGTAAACGTCATCGACACCCCCGGCCACGTAGACTTCACC
ATCGAGGTGGAGCGTTCCCTGCGTGTGCTGGATGGCGCTGTCGTGGTGTTCTGTGGCACCTCCGGCGTAGAG
CCGCAGTCGGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTACCGCGCATCGTCTACGTCAACAAGATG
GACCGCCAGGGCGCGAACTTCCTGCGCGTCGTCGAGCAGATCAAGAAGCGCCTGGGTCATACCCCGGTTCCG
GTCCAGCTCGCCATCGGCGCTGAAGAGAACTTCGTCGGTCAGGTCGACCTGATCAAGATGAAGGCCATCTAC
TGGAACGATGACGACAAGGGCATGACCTACCGCGAGGAAGAAATTCCTGCGGAGCTCAAGGACCTGGCCGAA
GAGTGGCGTTCCAGCATGGTCGAGGCTGCGGCCGAAGCCAACGAAGAGCTGATGAACAAGTACCTCGAAGAG
GGCGAGCTCTCCGAAGCCGAGATCAAGGAAGGCCTGCGTCTGCGCACCCTGGCGTGCGAGATCGTTCCGGCC
GTCTGCGGTTCCTCGTTCAAGAACAAGGGCGTTCCGCTGGTTCTCGATGCCGTCATCGACTACCTCCCGGCT
CCGACCGAGATCCCTGCGATCAAGGGCGTCAGCCCGGACGACGAGACCGTCGAAGACGAGCGTCATGCCGAC
GACAACGAACCGTTCTCGTCCCTGGCGTTCAAGATCGCTACCGACCCGTTCGTCGGTACCCTGACCTTCGCC
CGCGTTTACTCCGGCGTCCTGAGTTCCGGCGACTCCGTCCTGAACTCGGTGAAAGGCAAGAAAGAGCGCGTC
GGTCGGATGGTGCAGATGCACGCCAACCAGCGTGAAGAGATCAAGGAAGTGCGCGCAGGCGACATCGCTGCC
CTGATCGGCATGAAGGACGTCACCACCGGTGACACCCTGTGCTCGATCGAGAAGCCGATCATCCTCGAGCGC
ATGGACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGT
ATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACC
ATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAG
GCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGAAGGCAAG
TTCGTTCGCCAGTCCGGTGGTCGCGGTCAGTTCGGTCATTGCTGGATCCGTTTCTCGGCCGCGGACGTGGAC
GAGAAGGGCAACATCACCGAAGGCCTGGTGTTCGAGAACGAAGTCGTGGGCGGTGTGGTTCCGAAGGAATAC
ATCCCGGCGATCCAGAAGGGTATCGAAGAGCAGATGAAGAACGGCGTCGTTGCCGGCTATCCGCTCATCGGC
CTGAAGGCTACCGTTTTCGATGGTTCCTACCATGACGTCGACTCCAACGAGATGGCGTTCAAGATCGCTGCT
TCCATGGCGACCAAGCAGCTGGCCCAGAAGGGCGGCGGCAAGGTGCTCGAGCCGATCATGAAGGTAGAGGTG
GTGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATG
GAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGAC
GTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAAC
ATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGA
Translation:
MARTTPINRYRNIGICAHVDAGKTTTTERVLFYTGVNHKLGEVHDGAATTDWMVQEQERGITITSAAVTTFW
KGSRGQYDNYRVNVIDTPGHVDFTIEVERSLRVLDGAVVVFCGTSGVEPQSETVWRQANKYGVPRIVYVNKM
DRQGANFLRVVEQIKKRLGHTPVPVQLAIGAEENFVGQVDLIKMKAIYWNDDDKGMTYREEEIPAELKDLAE
EWRSSMVEAAAEANEELMNKYLEEGELSEAEIKEGLRLRTLACEIVPAVCGSSFKNKGVPLVLDAVIDYLPA
PTEIPAIKGVSPDDETVEDERHADDNEPFSSLAFKIATDPFVGTLTFARVYSGVLSSGDSVLNSVKGKKERV
GRMVQMHANQREEIKEVRAGDIAALIGMKDVTTGDTLCSIEKPIILERMDFPEPVISVAVEPKTKADQEKMG
IALGKLAQEDPSFRVKTDEESGQTIISGMGELHLDIIVDRMKREFGVEANIGKPQVAYRETITKDNVEIEGK
FVRQSGGRGQFGHCWIRFSAADVDEKGNITEGLVFENEVVGGVVPKEYIPAIQKGIEEQMKNGVVAGYPLIG
LKATVFDGSYHDVDSNEMAFKIAASMATKQLAQKGGGKVLEPIMKVEVVTPEDYMGDVMGDLNRRRGLIQGM
EDTVSGKVIRAEVPLGEMFGYATDVRSMSQGRASYSMEFSKYAEAPSNIVEALVKKQG*
AnnotationID:296GeneID:286
AnnotatorUID: diggins
Modification Date/Time: 2005-04-03 16:04:35
Gene Name:fusA1
Confidence Code:2
GeneProduct:elongation factor G
Cell Localization:()
Synonyms:fusA.fusA2
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(24) Transcription, RNA processing and degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0480,COG4108,pfam03764
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66081.1|AE016237_5 (AE016775) translation elongation factor G [Pseudomonas putida
           KT2440]
          Length = 715

 Score = 1283 bits (3319), Expect = 0.0
 Identities = 641/715 (89%), Positives = 676/715 (94%), Gaps = 9/715 (1%)

>gb|AAO54165.1| (AE016858) translation elongation factor G [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 701

 Score = 1190 bits (3079), Expect = 0.0
 Identities = 602/707 (85%), Positives = 646/707 (91%), Gaps = 7/707 (0%)

>gb|AAO09792.1|AE016801_111 (AE016801) Translation elongation factor [Vibrio vulnificus CMCP6]
          Length = 699

 Score = 1051 bits (2717), Expect = 0.0
 Identities = 521/707 (73%), Positives = 614/707 (86%), Gaps = 10/707 (1%)






 
Structural Features:
COG0480, FusA, Translation elongation factors (GTPases) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 697 residues,  99.3% aligned
Score =  982 bits (2540), Expect = 0.0
Identities = 458/708 (64%), Positives = 547/708 (77%), Gaps = 18/708 (2%)

COG4108, PrfC, Peptide chain release factor RF-3 
[Translation, ribosomal structure and biogenesis]
CD-Length = 528 residues,  91.3% aligned
Score =  226 bits (577), Expect = 9e-60
Identities = 144/512 (28%), Positives = 232/512 (45%), Gaps = 37/512 (7%)

pfam03764, EFG_IV, Elongation factor G, domain IV. 
CD-Length = 121 residues, 100.0% aligned
Score =  149 bits (379), Expect = 8e-37
Identities = 59/127 (46%), Positives = 75/127 (59%), Gaps = 6/127 (4%)






   
Genomic Context:






   
Comment:
other possible gene name: fus


















    ReferenceID:14101




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17717




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36636




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:286_cdd.html


    		




 
URL:

















    ReferenceID:36762




  
Author/Investigator(s):
        Zengel JM, Archer RH, Lindahl L.





  
Title:
        The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G.




  
PubMed:
        6322136


MedLine:
        




    
Source:
Nucleic Acids Res. 1984 Feb 24;12(4):2181-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6322136









           
Homologs By Global Alignment 

           

       



     
Gene ID:286
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
471
                           PA4266_tr
                           translation of PA4266
                           706
                           99.99
                           99.99
                           0
                           3619.0
     

     
     
24496
                           gb|AAG07654.1|AE004842_3
                           (AE004842) elongation factor G [Pseudomonas aeruginosa PAO1]
                           706
                           99.85
                           99.99
                           0
                           3616.0
     

     
     
24497
                           gb|AAN66081.1|AE016237_5
                           (AE016775) translation elongation factor G [Pseudomonas putida KT2440]
                           715
                           89.51
                           94.54
                           9
                           3322.0
     

     
     
24498
                           gb|AAO54165.1|
                           (AE016858) translation elongation factor G [Pseudomonas syringae pv. tomato str. DC3000]
                           707
                           85.00
                           91.37
                           7
                           3083.0
     

     
     
472
                           PA2071_tr
                           translation of PA2071
                           708
                           83.89
                           91.52
                           8
                           3072.0
     

     






      

          
GC ORFID: 43288How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4266Glimmer Score: 

          
Start: 4198274Stop: 4196154

          
Length: 2121


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4266 translation of PA4266Homolog Bit Score 1398.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5181 (ORF: BLASTX 1187.0), t_PA2071 (ORF: BLASTX 1184.0), t_PA4266 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGCCCGTACTACACCCATCAACCGCTACCGTAATATCGGTATCTGTGCCCACGTTGACGCGGGCAAGACC 
ACCACCACGGAGCGGGTTCTGTTCTATACCGGCGTCAACCACAAGCTGGGCGAAGTGCATGACGGCGCTGCG 
ACCACCGACTGGATGGTGCAGGAGCAGGAGCGCGGTATCACCATTACCTCCGCTGCCGTTACCACCTTCTGG 
AAGGGCTCGCGTGGTCAGTACGACAACTATCGCGTAAACGTCATCGACACCCCCGGCCACGTAGACTTCACC 
ATCGAGGTGGAGCGTTCCCTGCGTGTGCTGGATGGCGCTGTCGTGGTGTTCTGTGGCACCTCCGGCGTAGAG 
CCGCAGTCGGAAACCGTATGGCGTCAGGCCAACAAGTACGGCGTACCGCGCATCGTCTACGTCAACAAGATG 
GACCGCCAGGGCGCGAACTTCCTGCGCGTCGTCGAGCAGATCAAGAAGCGCCTGGGTCATACCCCGGTTCCG 
GTCCAGCTCGCCATCGGCGCTGAAGAGAACTTCGTCGGTCAGGTCGACCTGATCAAGATGAAGGCCATCTAC 
TGGAACGATGACGACAAGGGCATGACCTACCGCGAGGAAGAAATTCCTGCGGAGCTCAAGGACCTGGCCGAA 
GAGTGGCGTTCCAGCATGGTCGAGGCTGCGGCCGAAGCCAACGAAGAGCTGATGAACAAGTACCTCGAAGAG 
GGCGAGCTCTCCGAAGCCGAGATCAAGGAAGGCCTGCGTCTGCGCACCCTGGCGTGCGAGATCGTTCCGGCC 
GTCTGCGGTTCCTCGTTCAAGAACAAGGGCGTTCCGCTGGTTCTCGATGCCGTCATCGACTACCTCCCGGCT 
CCGACCGAGATCCCTGCGATCAAGGGCGTCAGCCCGGACGACGAGACCGTCGAAGACGAGCGTCATGCCGAC 
GACAACGAACCGTTCTCGTCCCTGGCGTTCAAGATCGCTACCGACCCGTTCGTCGGTACCCTGACCTTCGCC 
CGCGTTTACTCCGGCGTCCTGAGTTCCGGCGACTCCGTCCTGAACTCGGTGAAAGGCAAGAAAGAGCGCGTC 
GGTCGGATGGTGCAGATGCACGCCAACCAGCGTGAAGAGATCAAGGAAGTGCGCGCAGGCGACATCGCTGCC 
CTGATCGGCATGAAGGACGTCACCACCGGTGACACCCTGTGCTCGATCGAGAAGCCGATCATCCTCGAGCGC 
ATGGACTTCCCCGAGCCGGTAATCTCGGTCGCTGTAGAGCCGAAGACCAAGGCTGACCAGGAGAAGATGGGT 
ATCGCCCTCGGCAAGCTGGCCCAGGAAGACCCGTCGTTCCGTGTCAAGACCGACGAAGAGTCCGGTCAGACC 
ATCATCTCCGGCATGGGCGAGTTGCACCTGGACATCATCGTCGACCGCATGAAGCGCGAGTTCGGTGTCGAG 
GCCAACATCGGCAAGCCGCAGGTTGCCTACCGTGAGACCATCACCAAGGACAACGTGGAGATCGAAGGCAAG 
TTCGTTCGCCAGTCCGGTGGTCGCGGTCAGTTCGGTCATTGCTGGATCCGTTTCTCGGCCGCGGACGTGGAC 
GAGAAGGGCAACATCACCGAAGGCCTGGTGTTCGAGAACGAAGTCGTGGGCGGTGTGGTTCCGAAGGAATAC 
ATCCCGGCGATCCAGAAGGGTATCGAAGAGCAGATGAAGAACGGCGTCGTTGCCGGCTATCCGCTCATCGGC 
CTGAAGGCTACCGTTTTCGATGGTTCCTACCATGACGTCGACTCCAACGAGATGGCGTTCAAGATCGCTGCT 
TCCATGGCGACCAAGCAGCTGGCCCAGAAGGGCGGCGGCAAGGTGCTCGAGCCGATCATGAAGGTAGAGGTG 
GTGACTCCTGAGGACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATG 
GAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGAC 
GTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAAC 
ATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGA