ViewGene
Locus Name:
GeneID:
Location:

GeneID:2854
TrivialName: PA14_62850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62850
PAO1 Ortholog Locus:PA4750
Sequence Length:852
Protein Length:283
Start: 5607701
Stop: 5606850
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08136.1|AE004888_11 (AE004888) dihydropteroate synthase [Pseudomonas aeruginosa PAO1]
Identities = 282/283 (99%), Positives = 282/283 (99%)
Sequence:
ATGAGCAATCCGCAATACCCGACCCGACTGCCTTGCGGCAGTCGGGTTCTTGATTTGGCCCGCCCACATGTC
ATGGGCATCCTCAATGTCACCCCCGATTCCTTTTCCGACGGCGGCCGTTACGACCGGCTGGACGATGCCCTG
CGGCATGCCGAGCGCATGGTGCTGGAAGGCGCAACGCTCATCGACGTCGGCGGCGAGTCGACCCGTCCCGGT
GCGCGCAGCGTTTCTCCGACGGAGGAGCTGGAGCGGGTCGCTCCGGTAGTCGAACGCATCGCCCGCGAACTG
GACGTCGTGATTTCGGTGGATACCTCCACGCCTGCGGTCATGCGCGAAACTGCGCGTCTCGGAGCGGGTCTG
ATCAACGACGTCCGTTCGCTGCAGCGCGACGGTGCGCTGGATGCTGCGGTGGATAGCGGCCTGGCGGTCTGT
CTCATGCACATGCGCGGAGAACCGCAGACCATGCAGGATGCGCCTGCCTACCAGGATGTGGCGGCGGAAGTC
GGCGCGTTCCTGCAGGAGCGTGTGGATGCCTGCGTGGCGGCCGGAATCGACCGCGAGCGGCTGTTACTGGAT
CCGGGTTTCGGTTTTGCCAAGACACTGGAGCACAACCTGGTGCTGTTCAGGCATCTGCAGGCGCTCCATGGC
CTGGGCCTGCCGTTGTTGGTCGGCGTTTCGCGCAAGAGCATGATCGGCAAGGTACTGGGGCGGGAGGTGGAC
GAACGCCTCTACGGCAGTCTCGCGCTGGCGGCCCTGGCGCTGGCCAAGGGCGCACGGATAATTCGAGTGCAT
GACGTGGCGGCGACCGTCGACGTGATGCGAATGATCTCCGCCGTTGAAATGGCGGATTGA
Translation:
MSNPQYPTRLPCGSRVLDLARPHVMGILNVTPDSFSDGGRYDRLDDALRHAERMVLEGATLIDVGGESTRPG
ARSVSPTEELERVAPVVERIARELDVVISVDTSTPAVMRETARLGAGLINDVRSLQRDGALDAAVDSGLAVC
LMHMRGEPQTMQDAPAYQDVAAEVGAFLQERVDACVAAGIDRERLLLDPGFGFAKTLEHNLVLFRHLQALHG
LGLPLLVGVSRKSMIGKVLGREVDERLYGSLALAALALAKGARIIRVHDVAATVDVMRMISAVEMAD*
AnnotationID:2862GeneID:2854
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 07:07:54
Gene Name:folP
Confidence Code:2
GeneProduct:dihydropteroate synthase
Cell Localization:()
Synonyms:dhpS
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00739,cd00423,COG0294,pfam00809
Secondary Functional Category(ies):
EC Number:2.5.1.15
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70289.1|AE016669_7 (AE016791) dihydropteroate synthase [Pseudomonas putida KT2440]
          Length = 283

 Score =  423 bits (1087), Expect = e-118
 Identities = 214/283 (75%), Positives = 244/283 (86%)

>gb|AAO57944.1| (AE016872) dihydropteroate synthase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 283

 Score =  422 bits (1085), Expect = e-117
 Identities = 215/283 (75%), Positives = 243/283 (85%)

>gb|AAP18497.1| (AE016989) 7,8-dihydropteroate synthase [Shigella flexneri 2a str.
           2457T]
          Length = 297

 Score =  313 bits (801), Expect = 9e-85
 Identities = 160/262 (61%), Positives = 195/262 (74%)








 
Structural Features:
cd00739, DHPS, DHPS subgroup of Pterin binding enzymes. 
CD-Length = 257 residues,  99.6% aligned
Score =  324 bits (831), Expect = 1e-89
Identities = 147/256 (57%), Positives = 182/256 (71%), Gaps = 1/256 (0%)

cd00423, Pterin_binding, Pterin binding enzymes.
CD-Length = 258 residues,  99.2% aligned
Score =  274 bits (702), Expect = 8e-75
Identities = 139/256 (54%), Positives = 165/256 (64%), Gaps = 2/256 (0%)

COG0294, FolP, Dihydropteroate synthase and related enzymes [Coenzyme metabolism]
CD-Length = 274 residues,  95.6% aligned
Score =  272 bits (696), Expect = 4e-74
Identities = 139/266 (52%), Positives = 172/266 (64%), Gaps = 7/266 (2%)

pfam00809, Pterin_bind, Pterin binding enzyme. 
CD-Length = 213 residues, 100.0% aligned
Score =  220 bits (561), Expect = 2e-58
Identities = 115/213 (53%), Positives = 137/213 (64%), Gaps = 4/213 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5711




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22854




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36559




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2854_cdd.html


    		




 
URL:

















    ReferenceID:36612




  
Author/Investigator(s):
        Dallas WS, Dev IK, Ray PH.





  
Title:
        The dihydropteroate synthase gene, folP, is near the leucine tRNA gene, leuU, on the Escherichia coli chromosome.




  
PubMed:
        8244950


MedLine:
        




    
Source:
J Bacteriol. 1993 Dec;175(23):7743-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8244950

















    ReferenceID:36613




  
Author/Investigator(s):
        Dallas WS, Dev IK, Ray PH.





  
Title:
        The dihydropteroate synthase gene, folP, is near the leucine tRNA gene, leuU, on the Escherichia coli chromosome.




  
PubMed:
        8244950


MedLine:
        




    
Source:
J Bacteriol. 1993 Dec;175(23):7743-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8244950

















    ReferenceID:36614




  
Author/Investigator(s):
        Dallas WS, Gowen JE, Ray PH, Cox MJ, Dev IK.





  
Title:
        Cloning, sequencing, and enhanced expression of the dihydropteroate synthase gene of Escherichia coli MC4100.




  
PubMed:
        1522070


MedLine:
        




    
Source:
J Bacteriol. 1992 Sep;174(18):5961-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1522070

















    ReferenceID:36615




  
Author/Investigator(s):
        Talarico TL, Dev IK, Dallas WS, Ferone R, Ray PH.





  
Title:
        Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100.




  
PubMed:
        1657875


MedLine:
        




    
Source:
J Bacteriol. 1991 Nov;173(21):7029-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1657875









           
Homologs By Global Alignment 

           

       



     
Gene ID:2854
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4622
                           PA4750_tr
                           translation of PA4750
                           283
                           99.64
                           99.64
                           0
                           1409.0
     

     
     
132934
                           gb|AAG08136.1|AE004888_11
                           (AE004888) dihydropteroate synthase [Pseudomonas aeruginosa PAO1]
                           283
                           99.64
                           99.64
                           0
                           1409.0
     

     
     
132936
                           gb|AAO57944.1|
                           (AE016872) dihydropteroate synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           283
                           75.97
                           85.86
                           0
                           1085.0
     

     
     
132935
                           gb|AAN70289.1|AE016669_7
                           (AE016791) dihydropteroate synthase [Pseudomonas putida KT2440]
                           283
                           75.61
                           86.21
                           0
                           1087.0
     

     
     
132938
                           gb|AAL89711.1|AF483270_1
                           (AF483270) dihydropteroate synthase [Escherichia coli]
                           289
                           56.05
                           68.51
                           13
                           796.0
     

     






      

          
GC ORFID: 45856How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4750Glimmer Score: 

          
Start: 4871381Stop: 4870530

          
Length: 852


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4750 translation of PA4750Homolog Bit Score 547.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5486 (ORF: BLASTX 412.0), t_PA4750 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAATCCGCAATACCCGACCCGACTGCCTTGCGGCAGTCGGGTTCTTGATTTGGCCCGCCCACATGTC 
ATGGGCATCCTCAATGTCACCCCCGATTCCTTTTCCGACGGCGGCCGTTACGACCGGCTGGACGATGCCCTG 
CGGCATGCCGAGCGCATGGTGCTGGAAGGCGCAACGCTCATCGACGTCGGCGGCGAGTCGACCCGTCCCGGT 
GCGCGCAGCGTTTCTCCGACGGAGGAGCTGGAGCGGGTCGCTCCGGTAGTCGAACGCATCGCCCGCGAACTG 
GACGTCGTGATTTCGGTGGATACCTCCACGCCTGCGGTCATGCGCGAAACTGCGCGTCTCGGAGCGGGTCTG 
ATCAACGACGTCCGTTCGCTGCAGCGCGACGGTGCGCTGGATGCTGCGGTGGATAGCGGCCTGGCGGTCTGT 
CTCATGCACATGCGCGGAGAACCGCAGACCATGCAGGATGCGCCTGCCTACCAGGATGTGGCGGCGGAAGTC 
GGCGCGTTCCTGCAGGAGCGTGTGGATGCCTGCGTGGCGGCCGGAATCGACCGCGAGCGGCTGTTACTGGAT 
CCGGGTTTCGGTTTTGCCAAGACACTGGAGCACAACCTGGTGCTGTTCAGGCATCTGCAGGCGCTCCATGGC 
CTGGGCCTGCCGTTGTTGGTCGGCGTTTCGCGCAAGAGCATGATCGGCAAGGTACTGGGGCGGGAGGTGGAC 
GAACGCCTCTACGGCAGTCTCGCGCTGGCGGCCCTGGCGCTGGCCAAGGGCGCACGGATAATTCGAGTGCAT 
GACGTGGCGGCGACCGTCGACGTGATGCGAATGATCTCCGCCGTTGAAATGGCGGATTGA