ViewGene
Locus Name:
GeneID:
Location:

GeneID:2839
TrivialName: PA14_06660
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_06660
PAO1 Ortholog Locus:PA0510
Sequence Length:840
Protein Length:279
Start: 583399
Stop: 582560
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03899.1|AE004488_3 (AE004488) probable uroporphyrin-III c-methyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 278/279 (99%), Positives = 279/279 (100%)
Sequence:
ATGAACACTACCGTGATTCCGCCGTCCCTCCTCGATGTCGATTTCCCTGCCGGCAGCGTCGCCCTGGTCGGC
GCCGGCCCCGGCGATCCGGGCCTGCTCACCCTGCGCGCCTGGGCCCTGCTGCAACAGGCCGAGGTGGTGGTC
TACGACCGCCTGGTCGCCCGCGAGCTGATCGCCCTTCTGCCGGAAAGCTGCCAGCGCATCTACGTCGGCAAG
CGCTGCGGCCACCACAGCCTGCCGCAGGAAGAAATCAACGAGCTGCTGGTGCGCCTGGCCCGGCAGCAACGC
CGGGTGGTCCGCCTGAAAGGCGGCGACCCGTTCATCTTCGGTCGCGGCGCCGAGGAGCTGGAGCGCCTGCTC
GAGGCCGGCGTCGACTGCCAGGTGGTCCCCGGCGTCACCGCCGCCTCCGGTTGCAGTACCTACGCCGGCATC
CCGCTGACCCACCGCGACCTCGCCCAGTCCTGCACCTTCGTCACCGGCCACCTGCAGAACGACGGCCGCCTC
GACCTCGACTGGGCCGGCCTGGCGCGCGGCAAGCAGACCCTGGTGTTCTACATGGGCCTGGGCAACCTCGCC
GAGATCGCCGCGCGCCTGGTCGAGCACGGCCTCGCCAGCGACACCCCGGCGGCGCTGGTCAGCCAGGGCACC
CAGGCGGGCCAGCAGGTGACCCGCGGCGCCCTTTCCGAGCTGCCGGCGCTGGCGCGGCGCTACCAGTTGAAG
CCGCCGACGCTGATCGTCGTCGGCCAGGTGGTGGCGCTCTTCGCCGAGCGCGCGATGGCGCATCCGTCATAC
CTGGGCGCCGGCTCGCCGGTCAGCCGCGAGGCCGTCGCATGCGCCTGA
Translation:
MNTTVIPPSLLDVDFPAGSVALVGAGPGDPGLLTLRAWALLQQAEVVVYDRLVARELIALLPESCQRIYVGK
RCGHHSLPQEEINELLVRLARQQRRVVRLKGGDPFIFGRGAEELERLLEAGVDCQVVPGVTAASGCSTYAGI
PLTHRDLAQSCTFVTGHLQNDGRLDLDWAGLARGKQTLVFYMGLGNLAEIAARLVEHGLASDTPAALVSQGT
QAGQQVTRGALSELPALARRYQLKPPTLIVVGQVVALFAERAMAHPSYLGAGSPVSREAVACA*
AnnotationID:2847GeneID:2839
AnnotatorUID: diggins
Modification Date/Time: 2005-05-08 15:03:37
Gene Name:nirE
Confidence Code:2
GeneProduct:putative uroporphyrin-III c-methyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0007,pfam00590
Secondary Functional Category(ies):biosynthesis of cofactors, prosthetic groups and carriers
EC Number:2.1.1.107
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA98152.1| (Z73914) Uroporphyrinogen-III C-methyltransferase [Pseudomonas
           stutzeri]
          Length = 278

 Score =  350 bits (899), Expect = 4e-96
 Identities = 175/271 (64%), Positives = 206/271 (76%), Gaps = 1/271 (0%)

>gb|AAF95702.1| (AE004324) uroporphyrin-III C-methyltransferase [Vibrio cholerae]
          Length = 299

 Score =  285 bits (730), Expect = 2e-76
 Identities = 151/240 (62%), Positives = 180/240 (75%), Gaps = 3/240 (1%)

>gb|AAN69593.1|AE016593_1 (AE016788) uroporphyrin-III C-methyltransferase [Pseudomonas putida
           KT2440]
          Length = 463

 Score =  281 bits (719), Expect = 3e-75
 Identities = 145/236 (61%), Positives = 173/236 (73%), Gaps = 1/236 (0%)








 
Structural Features:
COG0007, CysG, Uroporphyrinogen-III methylase [Coenzyme metabolism]
CD-Length = 244 residues,  98.8% aligned
Score =  305 bits (783), Expect = 4e-84
Identities = 140/244 (57%), Positives = 168/244 (68%), Gaps = 3/244 (1%)

pfam00590, TP_methylase, Tetrapyrrole (Corrin/Porphyrin) Methylases.
CD-Length = 208 residues,  99.5% aligned
Score =  192 bits (490), Expect = 3e-50
Identities = 104/212 (49%), Positives = 133/212 (62%), Gaps = 5/212 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5681




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22824




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33818




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2839_cdd.html


    		




 
URL:

















    ReferenceID:35310




  
Author/Investigator(s):
        Kawasaki S, Arai H, Kodama T, Igarashi Y.





  
Title:
        Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d1 biosynthesis.




  
PubMed:
        8982003


MedLine:
        




    
Source:
J Bacteriol. 1997 Jan;179(1):235-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8982003









           
Homologs By Global Alignment 

           

       



     
Gene ID:2839
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4601
                           PA0510_tr
                           translation of PA0510
                           279
                           99.64
                           99.99
                           0
                           1417.0
     

     
     
132526
                           dbj|BAA12682.1|
                           (D84475) methyltransferase [Pseudomonas aeruginosa]
                           279
                           99.64
                           99.99
                           0
                           1417.0
     

     
     
132525
                           gb|AAG03899.1|AE004488_3
                           (AE004488) probable uroporphyrin-III c-methyltransferase [Pseudomonas aeruginosa PAO1]
                           279
                           99.64
                           99.99
                           0
                           1417.0
     

     
     
132527
                           emb|CAA98152.1|
                           (Z73914) Uroporphyrinogen-III C-methyltransferase [Pseudomonas stutzeri]
                           279
                           63.08
                           74.91
                           1
                           899.0
     

     
     
132528
                           gb|AAF95702.1|
                           (AE004324) uroporphyrin-III C-methyltransferase [Vibrio cholerae O1 biovar eltor str. N16961]
                           307
                           52.44
                           63.19
                           36
                           719.0
     

     






      

          
GC ORFID: 45841How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0510Glimmer Score: 

          
Start: 6384741Stop: 6383902

          
Length: 840


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0510 translation of PA0510Homolog Bit Score 550.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2817 (ORF: BLASTX 278.0), t_PA2611 (ORF: BLASTX 273.0), t_PA1778 (ORF: BLASTX 200.0), psyr_15may02_Scaffold1_revised_gene369 (ORF: BLASTX 178.0), t_PA0510 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACACTACCGTGATTCCGCCGTCCCTCCTCGATGTCGATTTCCCTGCCGGCAGCGTCGCCCTGGTCGGC 
GCCGGCCCCGGCGATCCGGGCCTGCTCACCCTGCGCGCCTGGGCCCTGCTGCAACAGGCCGAGGTGGTGGTC 
TACGACCGCCTGGTCGCCCGCGAGCTGATCGCCCTTCTGCCGGAAAGCTGCCAGCGCATCTACGTCGGCAAG 
CGCTGCGGCCACCACAGCCTGCCGCAGGAAGAAATCAACGAGCTGCTGGTGCGCCTGGCCCGGCAGCAACGC 
CGGGTGGTCCGCCTGAAAGGCGGCGACCCGTTCATCTTCGGTCGCGGCGCCGAGGAGCTGGAGCGCCTGCTC 
GAGGCCGGCGTCGACTGCCAGGTGGTCCCCGGCGTCACCGCCGCCTCCGGTTGCAGTACCTACGCCGGCATC 
CCGCTGACCCACCGCGACCTCGCCCAGTCCTGCACCTTCGTCACCGGCCACCTGCAGAACGACGGCCGCCTC 
GACCTCGACTGGGCCGGCCTGGCGCGCGGCAAGCAGACCCTGGTGTTCTACATGGGCCTGGGCAACCTCGCC 
GAGATCGCCGCGCGCCTGGTCGAGCACGGCCTCGCCAGCGACACCCCGGCGGCGCTGGTCAGCCAGGGCACC 
CAGGCGGGCCAGCAGGTGACCCGCGGCGCCCTTTCCGAGCTGCCGGCGCTGGCGCGGCGCTACCAGTTGAAG 
CCGCCGACGCTGATCGTCGTCGGCCAGGTGGTGGCGCTCTTCGCCGAGCGCGCGATGGCGCATCCGTCATAC 
CTGGGCGCCGGCTCGCCGGTCAGCCGCGAGGCCGTCGCATGCGCCTGA