ViewGene
Locus Name:
GeneID:
Location:

GeneID:2832
TrivialName: PA14_03020
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03020
PAO1 Ortholog Locus:PA0244
Sequence Length:855
Protein Length:284
Start: 267136
Stop: 266282
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03633.1|AE004462_6 (AE004462) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 282/284 (99%), Positives = 283/284 (99%)
Sequence:
ATGAGCGCGCCGCGATCGGTTCTCGCCGGCCTGATCGGTGCCGGCATCCAGGCGTCGCGGACCCCTGCCCTG
CACGAGCGCGAGGGCGACGCCCAGGGCATCCGCTATCTGTACCGGCTGATCGATCTCGACCCGCTGGGCAAG
TCGGCGGACGACCTGGAGTTCCTGCTCGCCGCCGCCAGCGACCTCGGCTTCACCGGGCTGAACGTCACCTTC
CCCTGCAAGCAGGCGATCATCCCCCTGCTCGACGAACTCTCCGCCGAGGCGCGCGGCATCGGCGCGGTGAAC
ACCGTGGTGCTGCGCGATGGCCGGCGCATCGGCCACAACACCGACTGCCTGGGCTTCGCCGAGGGCTTTCGG
CGCGGCCTGGCCGACGCGCCGCGCCAGCGCGTGGTGCAGATGGGCGCGGGCGGCGCCGGCGCGGCAGTGGCC
CACGCGCTGCTCGCCGAGGGCGTCGAGCGGCTGGTCCTGTTCGACGTCGACCCGGCGCGGGCCCAGGCCCTC
GCGGACAACCTCAACCAGCACTTCGGCGCGCCACGAGCGCTCGCCGGCAGCGACCTGGCCGGCGCCCTGGGC
GACGCCCAGGGATTGGTCAACACCACCCCGGTGGGCATGGCCAAGCTGCCCGGCATGCCGGTGCCGGCGGAA
CTGTTGCGCGCCGACCTGTGGGTCGCCGAGGTGATCTACTTTCCGCTGGAAACCGAGCTGCTGCGCCAGGCC
CGCGCCCTCGGCTGCCGCACACTGGACGGCGGCACCATGGCGGTGTTCCAGGCGGTGAAGGCATTCGAACTG
TTCAGCGGAGTAGAGGCTGACGCCGCGCGCATGCAGGCGCACTTCGCCAGCCTGGGCGACTGA
Translation:
MSAPRSVLAGLIGAGIQASRTPALHEREGDAQGIRYLYRLIDLDPLGKSADDLEFLLAAASDLGFTGLNVTF
PCKQAIIPLLDELSAEARGIGAVNTVVLRDGRRIGHNTDCLGFAEGFRRGLADAPRQRVVQMGAGGAGAAVA
HALLAEGVERLVLFDVDPARAQALADNLNQHFGAPRALAGSDLAGALGDAQGLVNTTPVGMAKLPGMPVPAE
LLRADLWVAEVIYFPLETELLRQARALGCRTLDGGTMAVFQAVKAFELFSGVEADAARMQAHFASLGD*
AnnotationID:2840GeneID:2832
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 19:07:13
Gene Name:
Confidence Code:3
GeneProduct:putative shikimate 5-dehydrogenase
Cell Localization:()
Synonyms:aroE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0169,pfam01488
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55860.1| (AE016864) shikimate 5-dehydrogenase, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 284

 Score =  422 bits (1085), Expect = e-117
 Identities = 211/277 (76%), Positives = 235/277 (84%)

>gb|AAN68018.1|AE016434_9 (AE016783) shikimate 5-dehydrogenase/quinate 5-dehydrogenase family
           protein [Pseudomonas putida KT2440]
          Length = 282

 Score =  420 bits (1080), Expect = e-117
 Identities = 214/277 (77%), Positives = 234/277 (84%)

>emb|CAD18549.1| (AL646084) PROBABLE SHIKIMATE 5-DEHYDROGENASE OXIDOREDUCTASE
           PROTEIN [Ralstonia solanacearum]
          Length = 315

 Score =  351 bits (901), Expect = 2e-96
 Identities = 179/279 (64%), Positives = 218/279 (78%)






 
Structural Features:
COG0169, AroE, Shikimate 5-dehydrogenase 
[Amino acid transport and metabolism]
CD-Length = 283 residues, 100.0% aligned
Score =  258 bits (660), Expect = 6e-70
Identities = 127/289 (43%), Positives = 168/289 (58%), Gaps = 11/289 (3%)

pfam01488, Shikimate_DH, Shikimate / quinate 5-dehydrogenase. 
CD-Length = 240 residues,  96.7% aligned
Score =  160 bits (405), Expect = 2e-40
Identities = 88/240 (36%), Positives = 130/240 (54%), Gaps = 12/240 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5667




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22810




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33603




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2832_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2832
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4579
                           PA0244_tr
                           translation of PA0244
                           284
                           99.29
                           99.64
                           0
                           1421.0
     

     
     
131746
                           gb|AAG03633.1|AE004462_6
                           (AE004462) hypothetical protein PA0244 [Pseudomonas aeruginosa PAO1]
                           284
                           99.29
                           99.64
                           0
                           1421.0
     

     
     
131748
                           gb|AAN68018.1|AE016434_9
                           (AE016783) shikimate 5-dehydrogenase/quinate 5-dehydrogenase family protein [Pseudomonas putida KT2440]
                           284
                           75.35
                           82.74
                           2
                           1077.0
     

     
     
131747
                           gb|AAO55860.1|
                           (AE016864) shikimate 5-dehydrogenase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           286
                           73.77
                           82.51
                           4
                           1080.0
     

     
     
131750
                           emb|CAC48440.1|
                           (AL603642) putative shikimate 5-dehydrogenase protein [Sinorhizobium meliloti]
                           286
                           61.53
                           72.72
                           2
                           857.0
     

     






      

          
GC ORFID: 45834How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0244Glimmer Score: 

          
Start: 6068477Stop: 6067623

          
Length: 855


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0244 translation of PA0244Homolog Bit Score 551.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene339 (ORF: BLASTX 426.0), t_PA0244 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGCGCCGCGATCGGTTCTCGCCGGCCTGATCGGTGCCGGCATCCAGGCGTCGCGGACCCCTGCCCTG 
CACGAGCGCGAGGGCGACGCCCAGGGCATCCGCTATCTGTACCGGCTGATCGATCTCGACCCGCTGGGCAAG 
TCGGCGGACGACCTGGAGTTCCTGCTCGCCGCCGCCAGCGACCTCGGCTTCACCGGGCTGAACGTCACCTTC 
CCCTGCAAGCAGGCGATCATCCCCCTGCTCGACGAACTCTCCGCCGAGGCGCGCGGCATCGGCGCGGTGAAC 
ACCGTGGTGCTGCGCGATGGCCGGCGCATCGGCCACAACACCGACTGCCTGGGCTTCGCCGAGGGCTTTCGG 
CGCGGCCTGGCCGACGCGCCGCGCCAGCGCGTGGTGCAGATGGGCGCGGGCGGCGCCGGCGCGGCAGTGGCC 
CACGCGCTGCTCGCCGAGGGCGTCGAGCGGCTGGTCCTGTTCGACGTCGACCCGGCGCGGGCCCAGGCCCTC 
GCGGACAACCTCAACCAGCACTTCGGCGCGCCACGAGCGCTCGCCGGCAGCGACCTGGCCGGCGCCCTGGGC 
GACGCCCAGGGATTGGTCAACACCACCCCGGTGGGCATGGCCAAGCTGCCCGGCATGCCGGTGCCGGCGGAA 
CTGTTGCGCGCCGACCTGTGGGTCGCCGAGGTGATCTACTTTCCGCTGGAAACCGAGCTGCTGCGCCAGGCC 
CGCGCCCTCGGCTGCCGCACACTGGACGGCGGCACCATGGCGGTGTTCCAGGCGGTGAAGGCATTCGAACTG 
TTCAGCGGAGTAGAGGCTGACGCCGCGCGCATGCAGGCGCACTTCGCCAGCCTGGGCGACTGA