ViewGene
Locus Name:
GeneID:
Location:

GeneID:2830
TrivialName: PA14_17980
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17980
PAO1 Ortholog Locus:PA3581
Sequence Length:840
Protein Length:279
Start: 1545204
Stop: 1544365
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06969.1|AE004779_1 (AE004779) glycerol uptake facilitator protein [Pseudomonas aeruginosa PAO1]
Identities = 279/279 (100%), Positives = 279/279 (100%)
Sequence:
ATGACCACCGCCGCCCCGACCCCGTCCCTGTTCGGCCAATGCCTGGCCGAATTCCTCGGCACCGCCCTCCTC
ATCTTCTTCGGCACTGGTTGCGTCGCGGCCCTGAAGGTCGCCGGCGCCAGCTTCGGCCTGTGGGAAATCAGC
ATCATCTGGGGGGTCGGCGTGAGCATGGCGATCTACCTCAGCGCCGGCGTCTCCGGCGCCCACCTGAACCCG
GCGGTGAGCATCGCGCTATGGCTGTTCGCCGGCTTCGAGGGGCGCAAGCTGCCGTTCTACATCACCGCCCAG
GTGGCCGGTGCGTTCTGCGCCGCAGCGCTGGTCTACACTCTCTACAGCAGCCTGTTCATCGAGTTCGAACAG
GCGCAGAACATCGTCCGCGGCAGCCAGGACAGCCTGGCCCTGGCCTCGGTGTTCTCCACCTATCCGCACCCG
GCGCTGTCGGTCGGCCAGGCGTTCCTCGTCGAAGTGGTGATCACCGCCATCCTCATGGCGGTGATCATGGCT
CTTACCGACGACGGCAACGGCCTGCCGCGCGGTCCGTTGGCGCCGCTGCTGATCGGCCTGCTGATCGCCGTG
ATCGGCAGCGCCATGGGCCCGCTGACCGGTTTCGCGATGAACCCGGCGCGCGACTTCGGCCCCAAGCTGATG
ACCTACCTGGCCGGCTGGGGGCCCATCGCCTTCACCGGCGGCCGCGAGATTCCCTACTTCCTGGTGCCGATC
TTCGCCCCGATCCTCGGTGCCTGCCTCGGCGCCGGCGGCTATCGTGTACTGATCGCGCGCCATCTGCCGAGC
GCCGCGGCGCCCGCCGAAGCCGAGCCGGAGAAGGTTCGCGCTTCCTGA
Translation:
MTTAAPTPSLFGQCLAEFLGTALLIFFGTGCVAALKVAGASFGLWEISIIWGVGVSMAIYLSAGVSGAHLNP
AVSIALWLFAGFEGRKLPFYITAQVAGAFCAAALVYTLYSSLFIEFEQAQNIVRGSQDSLALASVFSTYPHP
ALSVGQAFLVEVVITAILMAVIMALTDDGNGLPRGPLAPLLIGLLIAVIGSAMGPLTGFAMNPARDFGPKLM
TYLAGWGPIAFTGGREIPYFLVPIFAPILGACLGAGGYRVLIARHLPSAAAPAEAEPEKVRAS*
AnnotationID:2838GeneID:2830
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 09:09:06
Gene Name:glpF
Confidence Code:1
GeneProduct: glycerol uptake facilitator protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:glycerol dfiffusion facilitator
Functional Category Confidence Code:5
COGs:KOG0224,cd00333,COG0580
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66701.1|AE016298_5 (AE016778) glycerol uptake facilitator protein [Pseudomonas putida
           KT2440]
          Length = 283

 Score =  449 bits (1154), Expect = e-125
 Identities = 229/274 (83%), Positives = 245/274 (89%), Gaps = 3/274 (1%)

>gb|AAL22927.1| (AE008890) glycerol diffusion MIP channel [Salmonella typhimurium
           LT2]
          Length = 281

 Score =  393 bits (1010), Expect = e-109
 Identities = 193/257 (75%), Positives = 224/257 (87%)

>gb|AAN83307.1|AE016770_107 (AE016770) Glycerol uptake facilitator protein [Escherichia coli
           CFT073]
          Length = 281

 Score =  392 bits (1007), Expect = e-108
 Identities = 192/257 (74%), Positives = 224/257 (87%)








 
Structural Features:
KOG0224, Aquaporin (major intrinsic protein family) 
[Carbohydrate transport and metabolism]
CD-Length = 316 residues,  82.0% aligned
Score =  223 bits (569), Expect = 4e-59
Identities = 108/265 (40%), Positives = 155/265 (58%), Gaps = 7/265 (2%)

cd00333, MIP, Major intrinsic protein (MIP) superfamily.
CD-Length = 228 residues,  99.6% aligned
Score =  200 bits (509), Expect = 4e-52
Identities = 116/246 (47%), Positives = 147/246 (59%), Gaps = 20/246 (8%)

COG0580, GlpF, Glycerol uptake facilitator and related permeases 
(Major Intrinsic Protein Family) 
[Carbohydrate transport and metabolism]
CD-Length = 241 residues,  99.2% aligned
Score =  196 bits (499), Expect = 6e-51
Identities = 124/255 (48%), Positives = 156/255 (61%), Gaps = 19/255 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5663




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22806




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31961




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2830_cdd.html


    		




 
URL:

















    ReferenceID:34157




  
Author/Investigator(s):
        Schweizer HP, Jump R, Po C.





  
Title:
        Structure and gene-polypeptide relationships of the region encoding glycerol diffusion facilitator (glpF) and glycerol kinase (glpK) of Pseudomonas aeruginosa.




  
PubMed:
        9141691


MedLine:
        




    
Source:
Microbiology. 1997 Apr;143 ( Pt 4):1287-97.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9141691









           
Homologs By Global Alignment 

           

       



     
Gene ID:2830
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4577
                           PA3581_tr
                           translation of PA3581
                           279
                           99.99
                           99.99
                           0
                           1419.0
     

     
     
131512
                           gb|AAG06969.1|AE004779_1
                           (AE004779) glycerol uptake facilitator protein [Pseudomonas aeruginosa PAO1]
                           279
                           99.99
                           99.99
                           0
                           1419.0
     

     
     
131513
                           gb|AAB57803.1|
                           (U49666) glycerol dfiffusion facilitator [Pseudomonas aeruginosa]
                           279
                           99.28
                           99.64
                           0
                           1411.0
     

     
     
131515
                           gb|AAN66701.1|AE016298_5
                           (AE016778) glycerol uptake facilitator protein [Pseudomonas putida KT2440]
                           284
                           80.63
                           86.61
                           6
                           1155.0
     

     
     
131514
                           dbj|BAA31549.1|
                           (AB015973) glpF [Pseudomonas tolaasii]
                           285
                           80.35
                           85.61
                           6
                           1161.0
     

     






      

          
GC ORFID: 45832How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3581Glimmer Score: 

          
Start: 3408732Stop: 3409571

          
Length: 840


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3581 translation of PA3581Homolog Bit Score 551.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6125 (ORF: BLASTX 449.0), t_PA3581 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCACCGCCGCCCCGACCCCGTCCCTGTTCGGCCAATGCCTGGCCGAATTCCTCGGCACCGCCCTCCTC 
ATCTTCTTCGGCACTGGTTGCGTCGCGGCCCTGAAGGTCGCCGGCGCCAGCTTCGGCCTGTGGGAAATCAGC 
ATCATCTGGGGGGTCGGCGTGAGCATGGCGATCTACCTCAGCGCCGGCGTCTCCGGCGCCCACCTGAACCCG 
GCGGTGAGCATCGCGCTATGGCTGTTCGCCGGCTTCGAGGGGCGCAAGCTGCCGTTCTACATCACCGCCCAG 
GTGGCCGGTGCGTTCTGCGCCGCAGCGCTGGTCTACACTCTCTACAGCAGCCTGTTCATCGAGTTCGAACAG 
GCGCAGAACATCGTCCGCGGCAGCCAGGACAGCCTGGCCCTGGCCTCGGTGTTCTCCACCTATCCGCACCCG 
GCGCTGTCGGTCGGCCAGGCGTTCCTCGTCGAAGTGGTGATCACCGCCATCCTCATGGCGGTGATCATGGCT 
CTTACCGACGACGGCAACGGCCTGCCGCGCGGTCCGTTGGCGCCGCTGCTGATCGGCCTGCTGATCGCCGTG 
ATCGGCAGCGCCATGGGCCCGCTGACCGGTTTCGCGATGAACCCGGCGCGCGACTTCGGCCCCAAGCTGATG 
ACCTACCTGGCCGGCTGGGGGCCCATCGCCTTCACCGGCGGCCGCGAGATTCCCTACTTCCTGGTGCCGATC 
TTCGCCCCGATCCTCGGTGCCTGCCTCGGCGCCGGCGGCTATCGTGTACTGATCGCGCGCCATCTGCCGAGC 
GCCGCGGCGCCCGCCGAAGCCGAGCCGGAGAAGGTTCGCGCTTCCTGA