ViewGene
Locus Name:
GeneID:
Location:

GeneID:2809
TrivialName: PA14_41840
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41840
PAO1 Ortholog Locus:PA1756
Sequence Length:804
Protein Length:267
Start: 3738178
Stop: 3738981
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAB53743.1| (U95379) CysH [Pseudomonas aeruginosa]
Identities = 267/267 (100%), Positives = 267/267 (100%)
Sequence:
ATGCCGCCCTTTGCTACCATTCCCGCCACAGAGCGCAACAGCGCCGCCCAGCACCAGGACCCCAGCCCGATG
AGCCAGCCTTTCGACCTTCCCGCCCTCGCTTCCAGCCTTGCCGACAAATCCCCCCAGGACATCCTGAAAGCC
GCCTTCGAGCACTTCGGCGACGAGCTGTGGATCTCCTTCAGCGGCGCCGAGGACGTCGTCCTGGTAGACATG
GCCTGGAAGCTCAACCGCAACGTGAAGGTGTTCAGCCTCGACACCGGTCGCCTGCACCCGGAAACCTATCGT
TTCATCGACCAGGTCCGCGAACACTACGGCATCGCCATCGACGTGCTTTCCCCCGACCCGCGCCTGCTCGAA
CCCCTGGTGAAGGAAAAGGGCCTGTTCAGCTTCTACCGGGACGGCCACGGCGAGTGCTGCGGCATCCGCAAG
ATCGAACCGCTCAAGCGCAAGCTCGCCGGCGTGCGCGCCTGGGCCACCGGCCAACGCCGCGACCAGAGCCCC
GGCACGCGCAGCCAGGTGGCGGTGCTGGAAATCGACGGTGCCTTTTCCACGCCAGAAAAGCCGCTGTACAAG
TTCAACCCGCTGTCGTCCATGACCAGCGAGGAAGTCTGGGGCTATATCCGCATGCTCGAACTGCCCTACAAC
AGCCTGCACGAACGCGGCTATATCAGCATCGGCTGCGAACCCTGCACCCGTCCGGTGCTGCCCAACCAGCAC
GAGCGCGAGGGCCGCTGGTGGTGGGAGGAAGCCACCCACAAGGAGTGCGGGCTGCACGCCGGCAACCTGATC
AGCAAGGCCTGA
Translation:
MPPFATIPATERNSAAQHQDPSPMSQPFDLPALASSLADKSPQDILKAAFEHFGDELWISFSGAEDVVLVDM
AWKLNRNVKVFSLDTGRLHPETYRFIDQVREHYGIAIDVLSPDPRLLEPLVKEKGLFSFYRDGHGECCGIRK
IEPLKRKLAGVRAWATGQRRDQSPGTRSQVAVLEIDGAFSTPEKPLYKFNPLSSMTSEEVWGYIRMLELPYN
SLHERGYISIGCEPCTRPVLPNQHEREGRWWWEEATHKECGLHAGNLISKA*
AnnotationID:2817GeneID:2809
AnnotatorUID: diggins
Modification Date/Time: 2005-06-01 12:12:34
Gene Name:cysH
Confidence Code:2
GeneProduct:3'-phosphoadenosine-5'-phosphosulfate reductase
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:PAPS reductase
Functional Category Confidence Code:5
COGs:COG0175,pfam01507
Secondary Functional Category(ies):amino acid biosynthesis and metabolism
EC Number:1.8.4.8
Status:ACTIVE
Pathway:
Sulfate activation; cysteine biosynthesis reductive branch; third step.
Homology:
>gb|AAN67941.1|AE016426_6 (AE016782) phosphoadenosine phosphosulfate reductase [Pseudomonas
           putida KT2440]
          Length = 244

 Score =  447 bits (1150), Expect = e-125
 Identities = 209/244 (85%), Positives = 226/244 (92%)

>gb|AAO55794.1| (AE016864) phosphoadenosine phosphosulfate reductase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 244

 Score =  440 bits (1132), Expect = e-123
 Identities = 204/241 (84%), Positives = 222/241 (92%)
Structural Features:
COG0175, CysH, 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase 
(PAPS reductase)/FAD synthetase and related enzymes 
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 261 residues,  94.3% aligned
Score =  231 bits (590), Expect = 8e-62
Identities = 102/253 (40%), Positives = 137/253 (54%), Gaps = 11/253 (4%)

pfam01507, PAPS_reduct, Phosphoadenosine phosphosulfate reductase family. 
CD-Length = 197 residues,  99.5% aligned
Score =  212 bits (542), Expect = 3e-56
Identities = 80/202 (39%), Positives = 106/202 (52%), Gaps = 8/202 (3%)
Genomic Context:






   
Comment:


















    ReferenceID:5621




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22764




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37159




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2809_cdd.html


    		




 
URL:

















    ReferenceID:37832




  
Author/Investigator(s):
        Krone FA, Westphal G, Schwenn JD.





  
Title:
        Characterisation of the gene cysH and of its product phospho-adenylylsulphate reductase from Escherichia coli.




  
PubMed:
        2005873


MedLine:
        




    
Source:
Mol Gen Genet. 1991 Feb;225(2):314-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2005873









           
Homologs By Global Alignment 

           

       



     
Gene ID:2809
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
131030
                           gb|AAB53743.1|
                           (U95379) CysH [Pseudomonas aeruginosa]
                           267
                           99.99
                           99.99
                           0
                           1439.0
     

     
     
4554
                           PA1756_tr
                           translation of PA1756
                           267
                           99.62
                           99.62
                           0
                           1429.0
     

     
     
131031
                           gb|AAG05145.1|AE004601_10
                           (AE004601) 3'-phosphoadenosine-5'-phosphosulfate reductase [Pseudomonas aeruginosa PAO1]
                           267
                           99.62
                           99.62
                           0
                           1429.0
     

     
     
131032
                           gb|AAN67941.1|AE016426_6
                           (AE016782) phosphoadenosine phosphosulfate reductase [Pseudomonas putida KT2440]
                           267
                           78.27
                           84.64
                           23
                           1150.0
     

     
     
131033
                           gb|AAO55794.1|
                           (AE016864) phosphoadenosine phosphosulfate reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           267
                           76.40
                           83.52
                           23
                           1130.0
     

     






      

          
GC ORFID: 45811How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1756Glimmer Score: 

          
Start: 1215731Stop: 1214928

          
Length: 804


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1756 translation of PA1756Homolog Bit Score 555.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene380 (ORF: BLASTX 433.0), t_PA1756 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGCCCTTTGCTACCATTCCCGCCACAGAGCGCAACAGCGCCGCCCAGCACCAGGACCCCAGCCCGATG 
AGCCAGCCTTTCGACCTTCCCGCCCTCGCTTCCAGCCTTGCCGACAAATCCCCCCAGGACATCCTGAAAGCC 
GCCTTCGAGCACTTCGGCGACGAGCTGTGGATCTCCTTCAGCGGCGCCGAGGACGTCGTCCTGGTAGACATG 
GCCTGGAAGCTCAACCGCAACGTGAAGGTGTTCAGCCTCGACACCGGTCGCCTGCACCCGGAAACCTATCGT 
TTCATCGACCAGGTCCGCGAACACTACGGCATCGCCATCGACGTGCTTTCCCCCGACCCGCGCCTGCTCGAA 
CCCCTGGTGAAGGAAAAGGGCCTGTTCAGCTTCTACCGGGACGGCCACGGCGAGTGCTGCGGCATCCGCAAG 
ATCGAACCGCTCAAGCGCAAGCTCGCCGGCGTGCGCGCCTGGGCCACCGGCCAACGCCGCGACCAGAGCCCC 
GGCACGCGCAGCCAGGTGGCGGTGCTGGAAATCGACGGTGCCTTTTCCACGCCAGAAAAGCCGCTGTACAAG 
TTCAACCCGCTGTCGTCCATGACCAGCGAGGAAGTCTGGGGCTATATCCGCATGCTCGAACTGCCCTACAAC 
AGCCTGCACGAACGCGGCTATATCAGCATCGGCTGCGAACCCTGCACCCGTCCGGTGCTGCCCAACCAGCAC 
GAGCGCGAGGGCCGCTGGTGGTGGGAGGAAGCCACCCACAAGGAGTGCGGGCTGCACGCCGGCAACCTGATC 
AGCAAGGCCTGA