ViewGene
Locus Name:
GeneID:
Location:

GeneID:2808
TrivialName: PA14_04480
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04480
PAO1 Ortholog Locus:PA0342
Sequence Length:795
Protein Length:264
Start: 398905
Stop: 399699
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03731.1|AE004472_3 (AE004472) thymidylate synthase [Pseudomonas aeruginosa PAO1]
Identities = 264/264 (100%), Positives = 264/264 (100%)
Sequence:
ATGAAACAGTACCTCGACCTGATGCGCCACGTGCGCGAGCACGGCACCTTCAAGAGCGACCGCACCGGCACC
GGCACCTATAGCGTGTTCGGCCACCAGATGCGCTTCGACCTGGCCGCGGGCTTCCCCCTGGTGACCACCAAG
AAGTGCCATCTCAAGTCGATCGTCCACGAGCTGCTGTGGTTCCTCAAGGGCTCCACCAATATCGCCTACCTC
AAGGAGCACGGCGTCTCGATCTGGGACGAGTGGGCCGACGAGAACGGCGACCTCGGCCCGGTGTATGGCTAC
CAGTGGCGCTCCTGGCCGGCGCCGGACGGCCGCCACATCGACCAGATCGCCAACCTGATGGCGATGCTGAAG
AAGAATCCGGACTCGCGCCGGCTGATCGTCTCCGCCTGGAACCCGGCGCTGATCGACGAGATGGCCCTGCCG
CCCTGCCACGCGCTGTTCCAGTTCTACGTCGCCGACGGCAAGCTCAGCTGCCAGCTCTACCAGCGTTCGGCG
GACATCTTCCTCGGCGTACCCTTCAACATCGCCAGCTACGCTTTGCTGACCCTGATGGTGGCGCAGGTCGCC
GGCCTGCAGCCGGGCGAGTTCATCTGGACCGGCGGCGATTGCCACCTGTACGCCAACCACCTGGAGCAGGCC
GACCTGCAGTTGACCCGCGAGCCGCTGCCGTTGCCGAGCATGAAGCTGAATCCCGAGGTGAAGGATCTGTTC
GACTTCCGCTTCGAGGACTTCGAGCTGGTCGGCTACGAGGCCCATCCGCACATCAAGGCACCGGTCGCGGTC
TGA
Translation:
MKQYLDLMRHVREHGTFKSDRTGTGTYSVFGHQMRFDLAAGFPLVTTKKCHLKSIVHELLWFLKGSTNIAYL
KEHGVSIWDEWADENGDLGPVYGYQWRSWPAPDGRHIDQIANLMAMLKKNPDSRRLIVSAWNPALIDEMALP
PCHALFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTLMVAQVAGLQPGEFIWTGGDCHLYANHLEQA
DLQLTREPLPLPSMKLNPEVKDLFDFRFEDFELVGYEAHPHIKAPVAV*
AnnotationID:2816GeneID:2808
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 18:06:45
Gene Name:thyA
Confidence Code:2
GeneProduct:thymidylate synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00303
Secondary Functional Category(ies):
EC Number:2.1.1.45
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAF42056.1| (AE002521) thymidylate synthase [Neisseria meningitidis MC58]
          Length = 264

 Score =  459 bits (1180), Expect = e-128
 Identities = 215/264 (81%), Positives = 236/264 (89%)

>gb|AAC45469.1| (U86637) thymidylate synthase [Neisseria gonorrhoeae]
          Length = 267

 Score =  452 bits (1163), Expect = e-127
 Identities = 215/265 (81%), Positives = 235/265 (88%), Gaps = 1/265 (0%)

>gb|AAL51789.1| (AE009503) THYMIDYLATE SYNTHASE [Brucella melitensis 16M]
          Length = 264

 Score =  447 bits (1149), Expect = e-125
 Identities = 209/264 (79%), Positives = 233/264 (88%)






 
Structural Features:
pfam00303, Thymidylat_synt, Thymidylate synthase.
CD-Length = 280 residues,  99.3% aligned
Score =  427 bits (1099), Expect = 8e-121
Identities = 169/278 (60%), Positives = 202/278 (72%), Gaps = 14/278 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5619




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22762




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33683




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2808_cdd.html


    		




 
URL:

















    ReferenceID:35414




  
Author/Investigator(s):
        Tam NH, Borriss R.





  
Title:
        The thyA gene from Bacillus subtilis exhibits similarity with the phage phi 3T thymidylate synthase gene.




  
PubMed:
        7704257


MedLine:
        




    
Source:
Microbiology. 1995 Feb;141 ( Pt 2):291-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7704257

















    ReferenceID:35415




  
Author/Investigator(s):
        Nur-E-Kamal MS, al Mamun AA, Ahmed ZU.





  
Title:
        Molecular cloning of the wild-type and mutant thyA gene from Shigella flexneri Y.




  
PubMed:
        7935051


MedLine:
        




    
Source:
Microbiol Immunol. 1994;38(4):309-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7935051

















    ReferenceID:35416




  
Author/Investigator(s):
        Ross P, O'Gara F, Condon S.





  
Title:
        Cloning and characterization of the thymidylate synthase gene from Lactococcus lactis subsp. lactis.




  
PubMed:
        2117882


MedLine:
        




    
Source:
Appl Environ Microbiol. 1990 Jul;56(7):2156-63.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2117882

















    ReferenceID:35417




  
Author/Investigator(s):
        Finch PW, Wilson RE, Brown K, Hickson ID, Tomkinson AE, Emmerson PT.





  
Title:
        Complete nucleotide sequence of the Escherichia coli recC gene and of the thyA-recC intergenic region.




  
PubMed:
        3520484


MedLine:
        




    
Source:
Nucleic Acids Res. 1986 Jun 11;14(11):4437-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3520484









           
Homologs By Global Alignment 

           

       



     
Gene ID:2808
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4553
                           PA0342_tr
                           translation of PA0342
                           264
                           99.99
                           99.99
                           0
                           1431.0
     

     
     
130933
                           gb|AAG03731.1|AE004472_3
                           (AE004472) thymidylate synthase [Pseudomonas aeruginosa PAO1]
                           264
                           99.99
                           99.99
                           0
                           1431.0
     

     
     
130934
                           gb|AAQ58702.1|
                           (AE016913) thymidylate synthase [Chromobacterium violaceum ATCC 12472]
                           264
                           83.71
                           92.04
                           0
                           1216.0
     

     
     
130935
                           gb|AAF42056.1|
                           (AE002521) thymidylate synthase [Neisseria meningitidis MC58]
                           264
                           81.43
                           89.39
                           0
                           1180.0
     

     
     
130936
                           emb|CAB85183.1|
                           (AL162757) thymidylate synthase [Neisseria meningitidis Z2491]
                           264
                           81.43
                           89.39
                           0
                           1178.0
     

     






      

          
GC ORFID: 45810How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0342Glimmer Score: 

          
Start: 6200247Stop: 6201041

          
Length: 795


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0342 translation of PA0342Homolog Bit Score 555.0

          
Other Homologs:  t_PA0342 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACAGTACCTCGACCTGATGCGCCACGTGCGCGAGCACGGCACCTTCAAGAGCGACCGCACCGGCACC 
GGCACCTATAGCGTGTTCGGCCACCAGATGCGCTTCGACCTGGCCGCGGGCTTCCCCCTGGTGACCACCAAG 
AAGTGCCATCTCAAGTCGATCGTCCACGAGCTGCTGTGGTTCCTCAAGGGCTCCACCAATATCGCCTACCTC 
AAGGAGCACGGCGTCTCGATCTGGGACGAGTGGGCCGACGAGAACGGCGACCTCGGCCCGGTGTATGGCTAC 
CAGTGGCGCTCCTGGCCGGCGCCGGACGGCCGCCACATCGACCAGATCGCCAACCTGATGGCGATGCTGAAG 
AAGAATCCGGACTCGCGCCGGCTGATCGTCTCCGCCTGGAACCCGGCGCTGATCGACGAGATGGCCCTGCCG 
CCCTGCCACGCGCTGTTCCAGTTCTACGTCGCCGACGGCAAGCTCAGCTGCCAGCTCTACCAGCGTTCGGCG 
GACATCTTCCTCGGCGTACCCTTCAACATCGCCAGCTACGCTTTGCTGACCCTGATGGTGGCGCAGGTCGCC 
GGCCTGCAGCCGGGCGAGTTCATCTGGACCGGCGGCGATTGCCACCTGTACGCCAACCACCTGGAGCAGGCC 
GACCTGCAGTTGACCCGCGAGCCGCTGCCGTTGCCGAGCATGAAGCTGAATCCCGAGGTGAAGGATCTGTTC 
GACTTCCGCTTCGAGGACTTCGAGCTGGTCGGCTACGAGGCCCATCCGCACATCAAGGCACCGGTCGCGGTC 
TGA