ViewGene
Locus Name:
GeneID:
Location:

GeneID:280
TrivialName: PA14_20810
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20810
PAO1 Ortholog Locus:PA3344
Sequence Length:2139
Protein Length:712
Start: 1790076
Stop: 1792214
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06732.1|AE004757_1 (AE004757) ATP-dependent DNA helicase RecQ [Pseudomonas aeruginosa PAO1]
Identities = 712/712 (100%), Positives = 712/712 (100%)
Sequence:
ATGCGCGAGCAAGCCCTACGCATCCTCAAAGACGTTTTCGGTTACGACGCCTTCCGTGGCAACCAGGCACGG
ATCATCGAGCGGGTGGCCGAGGGTGGCGATGCGCTGGTACTGATGCCCACCGGCGGTGGCAAGTCCCTGTGT
TTCCAGGTCCCGGCGCTGCTGCGCGAAGGCCTGACGGTGGTGGTGTCGCCGCTGATCGCGCTGATGGAGGAC
CAGGTCGCCACCCTGGACGAACTCGGCGTGCCGGCGGTGGCGCTGAACTCCACCCTCAACCCCGAGCAGCAG
CGGGACATCGCCGAGCGCCTGCAGCGCGGCGAGATCAAGCTGCTCTACCTGGCACCGGAACGGTTGGTCCAG
CCGCGCATGCTGGCCTTCCTGCAACGACTGCCGGTCGGCCTGTTCGCCATCGACGAAGCGCACTGCGTGTCG
CAATGGGGCCATGACTTCCGTCCCGAATACCTGCAGCTCGGCCAGCTTGCCGAGCTGTTCCCGCAGGTGCCG
CGGATCGCCCTGACCGCCACCGCGGACATGCGTACCCGCGAGGAGATGATCCAGCGCCTGCACCTGCAGAAC
GCCGAGCAGTTCCTCTCCAGCTTCGACCGGCCGAACATCTTCTACCGCATCGTGCCCAAGGAGCAGCCGCGC
AAGCAGTTGCTCGGCTTCCTCTCCGAGCGGCGCGGCGATGCCGGCATCGTCTACTGCCTGTCGCGGAAGAAG
GTCGAGGAGGTCGCGGAATTCCTCGGCAACCAGGGCTTCCCCGCGCTGCCGTATCACGCCGGACTGTCCAAC
GAACTGCGTGCCCACCACCAGAAGCGCTTCCTCAACGAGGAAGGCTTGATCATGGTGGCGACCATCGCCTTC
GGCATGGGCATCGACAAGCCCAACGTGCGATTCGTCGCCCACCTCGACCTGCCCAAGAGCCTCGAGGCCTAT
TACCAGGAAACCGGCCGCGCCGGCCGCGACGGCCTGCCGGCCGACGCCTGGATGGCCTACGGCCTGCAGGAC
GTCCTGCTGCTGCGGCAGATGATGCAGAGTTCCGAGGGCGACGAGCGGCACAAGCGCGTCGAGCGGCACAAG
CTGGAAGCCATGCTGGCGCTCTGCGAGGAAACCCGCTGCCGGCGCCAGGCGCTGCTGGCCTATTTCGACGAG
GAGATGCCGCAGCCCTGCGGGCATTGCGACAACTGCGTGGACGGCGTGGAAACCTGGGATGCCACCGAATCC
GCGCGCCAGGCGCTGTCGGCGATCTACCGCAGCGGCCAGCGCTACGGCGTCGGCCATCTGGTGGATATCCTG
CTCGGCCGCGAGACCGAGAAGATCCGCAGTCTCGGCCACCAGCACCTGGCGGTGTTCGGGATCGGCAAGGGG
CGCGGCGAAGACGAGTGGCGGACCCTGTTtcGCCAGTTGGTCGCGCGCGGCCTGGCCGACGTCGACCTGGAC
GGCTTCGGCGGCCTGCGCCTGACCGAGGCCTGTCGTCCGCTGTTGCGTGGCGAGGTGCGGCTGGAGCTGCGC
CGCGACCTCAAGCCACAGCGCGCCAAGGGCTCCTCCAGCGGCGGCGCCAGCGCCGCCAGCCAACTGGTGCGC
AGCGAAGAGCGGGAAATGTGGGAGGCCCTGCGCGCGCTGCGGCGCAAGCTGGCCGAAGAGCATTCGGTGCCG
CCCTATGTGATCTTCCCCGACGCGACCCTGCTGGAAATGCTCCGCAGCCAGCCGCGCTCGCTATCCGACATG
GCCCAGGTCAGCGGGGTCGGCGCGCGCAAGCTGGAGCGCTACGGCCAGGCCTTCCTCGATGTCCTCACCGAC
TCGCCGGCGGCGCCCGCCGCGCCGCCGCAGGACCTGCGCCACGAATTGGCCAGCCTGGCCTGTGCGGGGATG
ACCCCGGCGCAGATAGCCCGCCAGCTCAATTGCAGCGAGAAGAACGTCTACGCGATGCTCGCCGAGGCCATC
GCCGGTCAGCAGGTGAGCCTGGAGCAGGCGCTGGATCTGCCCGAGGAACTCCTCGGCGAAATCCAGGACGCC
TTCCTCGAGGAAGACGGCGAACTGCCGCCGGTGGCGGCGCTGGAGGAGCGTTTCGGCAAGCGGGTGCCGAGT
GGCGTGCTGCACTGCGTGCGCGCCGCCCTGCAGGTGGAACTGGAGTCGTGA
Translation:
MREQALRILKDVFGYDAFRGNQARIIERVAEGGDALVLMPTGGGKSLCFQVPALLREGLTVVVSPLIALMED
QVATLDELGVPAVALNSTLNPEQQRDIAERLQRGEIKLLYLAPERLVQPRMLAFLQRLPVGLFAIDEAHCVS
QWGHDFRPEYLQLGQLAELFPQVPRIALTATADMRTREEMIQRLHLQNAEQFLSSFDRPNIFYRIVPKEQPR
KQLLGFLSERRGDAGIVYCLSRKKVEEVAEFLGNQGFPALPYHAGLSNELRAHHQKRFLNEEGLIMVATIAF
GMGIDKPNVRFVAHLDLPKSLEAYYQETGRAGRDGLPADAWMAYGLQDVLLLRQMMQSSEGDERHKRVERHK
LEAMLALCEETRCRRQALLAYFDEEMPQPCGHCDNCVDGVETWDATESARQALSAIYRSGQRYGVGHLVDIL
LGRETEKIRSLGHQHLAVFGIGKGRGEDEWRTLFRQLVARGLADVDLDGFGGLRLTEACRPLLRGEVRLELR
RDLKPQRAKGSSSGGASAASQLVRSEEREMWEALRALRRKLAEEHSVPPYVIFPDATLLEMLRSQPRSLSDM
AQVSGVGARKLERYGQAFLDVLTDSPAAPAAPPQDLRHELASLACAGMTPAQIARQLNCSEKNVYAMLAEAI
AGQQVSLEQALDLPEELLGEIQDAFLEEDGELPPVAALEERFGKRVPSGVLHCVRAALQVELES*
AnnotationID:290GeneID:280
AnnotatorUID: diggins
Modification Date/Time: 2005-04-15 10:10:41
Gene Name:recQ
Confidence Code:2
GeneProduct:ATP-dependent DNA helicase RecQ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0514
Secondary Functional Category(ies):
EC Number:3.6.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55164.1| (AE016861) ATP-dependent DNA helicase RecQ [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 709

 Score = 1156 bits (2990), Expect = 0.0
 Identities = 572/711 (80%), Positives = 637/711 (89%), Gaps = 3/711 (0%)

>gb|AAM42219.1| (AE012409) DNA helicase [Xanthomonas campestris pv. campestris str.
           ATCC 33913]
          Length = 600

 Score =  676 bits (1743), Expect = 0.0
 Identities = 339/600 (56%), Positives = 434/600 (72%), Gaps = 2/600 (0%)

>gb|AAO28489.1| (AE012555) ATP-dependent DNA helicase [Xylella fastidiosa
           Temecula1]
          Length = 600

 Score =  654 bits (1687), Expect = 0.0
 Identities = 330/592 (55%), Positives = 428/592 (72%), Gaps = 6/592 (1%)






 
Structural Features:
COG0514, RecQ, Superfamily II DNA helicase 
[DNA replication, recombination, and repair]
CD-Length = 590 residues,  99.8% aligned
Score =  744 bits (1921), Expect = 0.0
Identities = 313/605 (51%), Positives = 420/605 (69%), Gaps = 18/605 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:563




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17705




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32217




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:280_cdd.html


    		




 
URL:

















    ReferenceID:33996




  
Author/Investigator(s):
        Umezu K, Nakayama K, Nakayama H.





  
Title:
        Escherichia coli RecQ protein is a DNA helicase.




  
PubMed:
        2164680


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1990 Jul;87(14):5363-7.

Erratum in:
Proc Natl Acad Sci U S A 1990 Nov;87(22):9072.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2164680

















    ReferenceID:33997




  
Author/Investigator(s):
        Irino N, Nakayama K, Nakayama H.





  
Title:
        The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation.




  
PubMed:
        3027506


MedLine:
        




    
Source:
Mol Gen Genet. 1986 Nov;205(2):298-304.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3027506









           
Homologs By Global Alignment 

           

       



     
Gene ID:280
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
465
                           PA3344_tr
                           translation of PA3344
                           712
                           99.99
                           99.99
                           0
                           3641.0
     

     
     
24342
                           gb|AAG06732.1|AE004757_1
                           (AE004757) ATP-dependent DNA helicase RecQ [Pseudomonas aeruginosa PAO1]
                           712
                           99.99
                           99.99
                           0
                           3641.0
     

     
     
24343
                           gb|AAO55164.1|
                           (AE016861) ATP-dependent DNA helicase RecQ [Pseudomonas syringae pv. tomato str. DC3000]
                           712
                           80.33
                           89.46
                           3
                           2992.0
     

     
     
24344
                           gb|AAN70090.1|AE016648_8
                           (AE016790) ATP-dependent DNA helicase RecQ [Pseudomonas putida KT2440]
                           715
                           79.30
                           89.79
                           3
                           2964.0
     

     
     
24346
                           gb|AAM37969.1|
                           (AE011957) DNA helicase [Xanthomonas axonopodis pv. citri str. 306]
                           712
                           47.75
                           60.95
                           114
                           1735.0
     

     






      

          
GC ORFID: 43282How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3344Glimmer Score: 

          
Start: 3163856Stop: 3161718

          
Length: 2139


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3344 translation of PA3344Homolog Bit Score 1407.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6402 (ORF: BLASTX 1156.0), t_PA3344 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCGAGCAAGCCCTACGCATCCTCAAAGACGTTTTCGGTTACGACGCCTTCCGTGGCAACCAGGCACGG 
ATCATCGAGCGGGTGGCCGAGGGTGGCGATGCGCTGGTACTGATGCCCACCGGCGGTGGCAAGTCCCTGTGT 
TTCCAGGTCCCGGCGCTGCTGCGCGAAGGCCTGACGGTGGTGGTGTCGCCGCTGATCGCGCTGATGGAGGAC 
CAGGTCGCCACCCTGGACGAACTCGGCGTGCCGGCGGTGGCGCTGAACTCCACCCTCAACCCCGAGCAGCAG 
CGGGACATCGCCGAGCGCCTGCAGCGCGGCGAGATCAAGCTGCTCTACCTGGCACCGGAACGGTTGGTCCAG 
CCGCGCATGCTGGCCTTCCTGCAACGACTGCCGGTCGGCCTGTTCGCCATCGACGAAGCGCACTGCGTGTCG 
CAATGGGGCCATGACTTCCGTCCCGAATACCTGCAGCTCGGCCAGCTTGCCGAGCTGTTCCCGCAGGTGCCG 
CGGATCGCCCTGACCGCCACCGCGGACATGCGTACCCGCGAGGAGATGATCCAGCGCCTGCACCTGCAGAAC 
GCCGAGCAGTTCCTCTCCAGCTTCGACCGGCCGAACATCTTCTACCGCATCGTGCCCAAGGAGCAGCCGCGC 
AAGCAGTTGCTCGGCTTCCTCTCCGAGCGGCGCGGCGATGCCGGCATCGTCTACTGCCTGTCGCGGAAGAAG 
GTCGAGGAGGTCGCGGAATTCCTCGGCAACCAGGGCTTCCCCGCGCTGCCGTATCACGCCGGACTGTCCAAC 
GAACTGCGTGCCCACCACCAGAAGCGCTTCCTCAACGAGGAAGGCTTGATCATGGTGGCGACCATCGCCTTC 
GGCATGGGCATCGACAAGCCCAACGTGCGATTCGTCGCCCACCTCGACCTGCCCAAGAGCCTCGAGGCCTAT 
TACCAGGAAACCGGCCGCGCCGGCCGCGACGGCCTGCCGGCCGACGCCTGGATGGCCTACGGCCTGCAGGAC 
GTCCTGCTGCTGCGGCAGATGATGCAGAGTTCCGAGGGCGACGAGCGGCACAAGCGCGTCGAGCGGCACAAG 
CTGGAAGCCATGCTGGCGCTCTGCGAGGAAACCCGCTGCCGGCGCCAGGCGCTGCTGGCCTATTTCGACGAG 
GAGATGCCGCAGCCCTGCGGGCATTGCGACAACTGCGTGGACGGCGTGGAAACCTGGGATGCCACCGAATCC 
GCGCGCCAGGCGCTGTCGGCGATCTACCGCAGCGGCCAGCGCTACGGCGTCGGCCATCTGGTGGATATCCTG 
CTCGGCCGCGAGACCGAGAAGATCCGCAGTCTCGGCCACCAGCACCTGGCGGTGTTCGGGATCGGCAAGGGG 
CGCGGCGAAGACGAGTGGCGGACCCTGTTtcGCCAGTTGGTCGCGCGCGGCCTGGCCGACGTCGACCTGGAC 
GGCTTCGGCGGCCTGCGCCTGACCGAGGCCTGTCGTCCGCTGTTGCGTGGCGAGGTGCGGCTGGAGCTGCGC 
CGCGACCTCAAGCCACAGCGCGCCAAGGGCTCCTCCAGCGGCGGCGCCAGCGCCGCCAGCCAACTGGTGCGC 
AGCGAAGAGCGGGAAATGTGGGAGGCCCTGCGCGCGCTGCGGCGCAAGCTGGCCGAAGAGCATTCGGTGCCG 
CCCTATGTGATCTTCCCCGACGCGACCCTGCTGGAAATGCTCCGCAGCCAGCCGCGCTCGCTATCCGACATG 
GCCCAGGTCAGCGGGGTCGGCGCGCGCAAGCTGGAGCGCTACGGCCAGGCCTTCCTCGATGTCCTCACCGAC 
TCGCCGGCGGCGCCCGCCGCGCCGCCGCAGGACCTGCGCCACGAATTGGCCAGCCTGGCCTGTGCGGGGATG 
ACCCCGGCGCAGATAGCCCGCCAGCTCAATTGCAGCGAGAAGAACGTCTACGCGATGCTCGCCGAGGCCATC 
GCCGGTCAGCAGGTGAGCCTGGAGCAGGCGCTGGATCTGCCCGAGGAACTCCTCGGCGAAATCCAGGACGCC 
TTCCTCGAGGAAGACGGCGAACTGCCGCCGGTGGCGGCGCTGGAGGAGCGTTTCGGCAAGCGGGTGCCGAGT 
GGCGTGCTGCACTGCGTGCGCGCCGCCCTGCAGGTGGAACTGGAGTCGTGA