ViewGene
Locus Name:
GeneID:
Location:

GeneID:2799
TrivialName: PA14_61750
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_61750
PAO1 Ortholog Locus:PA4669
Sequence Length:849
Protein Length:282
Start: 5509336
Stop: 5510184
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08056.1|AE004880_12 (AE004880) isopentenyl monophosphate kinase [Pseudomonas aeruginosa PAO1]
Identities = 278/282 (98%), Positives = 280/282 (99%)
Sequence:
ATGAGCGTGCGCCTCAGCCTGCCAGCCCCGGCCAAGCTCAATCTGTTCCTGCATATCCTAGGCCGCCGCGAC
GATGGCTATCACGAGTTGCAGACCCTGTTCCAATTCCTCGACCACGGCGACGAGCTGCATTTCGAAGCCCGC
CAGGACGGCCAGGTACGCCTGCACACGGAAATCGCCGGGGTGCCGCACGACAGCAACCTGATCGTGCGCGCC
GCGCGCGGGCTGCAGGAAGCCTCCGGCAGCCTGCAAGGCGTCGACATCTGGCTGGACAAGCGCTTGCCCATG
GGGGGCGGCATCGGCGGCGGCAGCTCCGACGCCGCCACGACGCTCCTGGCCTTGAACCATCTCTGGCAACTC
GGCTGGGACGAGGACCGCATCGCCGCCCTGGGCCTGCGCCTGGGCGCCGACGTGCCGGTCTTCACCCGCGGA
CGCGCGGCTTTCGCCGAGGGCGTGGGCGAAAAGCTCACCCCGGTGGACATCCCGGAGCTCTGGTATCTCGTC
TTGGTTCCGCAAGTACTTGTCAGCACAGCAGAAATTTTTTCAGATCCACTGTTGACACGCGATTCTCCCGCC
ATTAAAGTGCGCACCGTTCTCGAGGGGGACAGCCGAAACGACTGTCAGCCGGTAGTCGAGAGGCGTTACCCA
GAAGTTCGTAACGCTTTGATCTTGCTAAACAAATTTGTTTCAGCTAGATTAACCGGCACTGGAGGTTGTGTG
TTTGGGAGCTTCCCAAACAAAGCTGAAGCTGATAAAGTCTCGGCCCTTCTTCCAGACCACCTTCAGAGGTTC
GTCGCCAAAGGAAGCAACGTCTCGATGTTGCACCGGAAGCTGGAAACTCTGGTCTGA
Translation:
MSVRLSLPAPAKLNLFLHILGRRDDGYHELQTLFQFLDHGDELHFEARQDGQVRLHTEIAGVPHDSNLIVRA
ARGLQEASGSLQGVDIWLDKRLPMGGGIGGGSSDAATTLLALNHLWQLGWDEDRIAALGLRLGADVPVFTRG
RAAFAEGVGEKLTPVDIPELWYLVLVPQVLVSTAEIFSDPLLTRDSPAIKVRTVLEGDSRNDCQPVVERRYP
EVRNALILLNKFVSARLTGTGGCVFGSFPNKAEADKVSALLPDHLQRFVAKGSNVSMLHRKLETLV*
AnnotationID:2807GeneID:2799
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 20:08:28
Gene Name:ipk
Confidence Code:2
GeneProduct:isopentenyl monophosphate kinase
Cell Localization:()
Synonyms:ispE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:4-diphosphocytidyl-2C-methyl-D-erythritol kinase
Functional Category Confidence Code:5
COGs:COG1947
Secondary Functional Category(ies):
EC Number:2.7.1.148
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54634.1| (AE016859) 4-diphosphocytidyl-2C-methyl-D-erythritol kinase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 282

 Score =  434 bits (1116), Expect = e-121
 Identities = 218/281 (77%), Positives = 238/281 (84%)

>gb|AAN66348.1|AE016263_5 (AE016776) 4-diphosphocytidyl-2C-methyl-D-erythritol kinase
           [Pseudomonas putida KT2440]
          Length = 286

 Score =  419 bits (1077), Expect = e-117
 Identities = 213/279 (76%), Positives = 233/279 (83%)

>emb|CAB64963.1| (AJ249325) isopentenyl monophosphate kinase [Escherichia coli]
          Length = 283

 Score =  300 bits (769), Expect = 5e-81
 Identities = 157/282 (55%), Positives = 195/282 (69%), Gaps = 6/282 (2%)








 
Structural Features:
COG1947, IspE, 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase 
[Lipid metabolism]
CD-Length = 289 residues,  99.7% aligned
Score =  326 bits (836), Expect = 3e-90
Identities = 139/288 (48%), Positives = 180/288 (62%), Gaps = 13/288 (4%)






   
Genomic Context:






   
Comment:
other possible gene name: ychB


















    ReferenceID:5601




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22744




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36554




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2799_cdd.html


    		




 
URL:

















    ReferenceID:36602




  
Author/Investigator(s):
        Freiberg C, Wieland B, Spaltmann F, Ehlert K, Brotz H, Labischinski H.





  
Title:
        Identification of novel essential Escherichia coli genes conserved among pathogenic bacteria.




  
PubMed:
        11361082


MedLine:
        




    
Source:
J Mol Microbiol Biotechnol. 2001 Jul;3(3):483-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11361082

















    ReferenceID:36603




  
Author/Investigator(s):
        Lange BM, Croteau R.





  
Title:
        Isopentenyl diphosphate biosynthesis via a mevalonate-independent pathway: isopentenyl monophosphate kinase catalyzes the terminal enzymatic step.




  
PubMed:
        10570138


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13714-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=10570138









           
Homologs By Global Alignment 

           

       



     
Gene ID:2799
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4542
                           PA4669_tr
                           translation of PA4669
                           282
                           98.58
                           99.29
                           0
                           1433.0
     

     
     
130644
                           gb|AAG08056.1|AE004880_12
                           (AE004880) isopentenyl monophosphate kinase [Pseudomonas aeruginosa PAO1]
                           282
                           98.58
                           99.29
                           0
                           1433.0
     

     
     
130645
                           emb|CAA57570.1|
                           (X82071) hypothetical protein [Pseudomonas aeruginosa]
                           282
                           77.30
                           77.65
                           61
                           1128.0
     

     
     
130646
                           gb|AAO54634.1|
                           (AE016859) 4-diphosphocytidyl-2C-methyl-D-erythritol kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           283
                           77.03
                           84.09
                           2
                           1116.0
     

     
     
130647
                           gb|AAN66348.1|AE016263_5
                           (AE016776) 4-diphosphocytidyl-2C-methyl-D-erythritol kinase [Pseudomonas putida KT2440]
                           287
                           74.21
                           81.18
                           6
                           1074.0
     

     






      

          
GC ORFID: 45801How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4669Glimmer Score: 

          
Start: 4773016Stop: 4773864

          
Length: 849


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4669 translation of PA4669Homolog Bit Score 556.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene5094 (ORF: BLASTX 442.0), t_PA4669 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGTGCGCCTCAGCCTGCCAGCCCCGGCCAAGCTCAATCTGTTCCTGCATATCCTAGGCCGCCGCGAC 
GATGGCTATCACGAGTTGCAGACCCTGTTCCAATTCCTCGACCACGGCGACGAGCTGCATTTCGAAGCCCGC 
CAGGACGGCCAGGTACGCCTGCACACGGAAATCGCCGGGGTGCCGCACGACAGCAACCTGATCGTGCGCGCC 
GCGCGCGGGCTGCAGGAAGCCTCCGGCAGCCTGCAAGGCGTCGACATCTGGCTGGACAAGCGCTTGCCCATG 
GGGGGCGGCATCGGCGGCGGCAGCTCCGACGCCGCCACGACGCTCCTGGCCTTGAACCATCTCTGGCAACTC 
GGCTGGGACGAGGACCGCATCGCCGCCCTGGGCCTGCGCCTGGGCGCCGACGTGCCGGTCTTCACCCGCGGA 
CGCGCGGCTTTCGCCGAGGGCGTGGGCGAAAAGCTCACCCCGGTGGACATCCCGGAGCTCTGGTATCTCGTC 
TTGGTTCCGCAAGTACTTGTCAGCACAGCAGAAATTTTTTCAGATCCACTGTTGACACGCGATTCTCCCGCC 
ATTAAAGTGCGCACCGTTCTCGAGGGGGACAGCCGAAACGACTGTCAGCCGGTAGTCGAGAGGCGTTACCCA 
GAAGTTCGTAACGCTTTGATCTTGCTAAACAAATTTGTTTCAGCTAGATTAACCGGCACTGGAGGTTGTGTG 
TTTGGGAGCTTCCCAAACAAAGCTGAAGCTGATAAAGTCTCGGCCCTTCTTCCAGACCACCTTCAGAGGTTC 
GTCGCCAAAGGAAGCAACGTCTCGATGTTGCACCGGAAGCTGGAAACTCTGGTCTGA