ViewGene
Locus Name:
GeneID:
Location:

GeneID:2780
TrivialName: PA14_65270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65270
PAO1 Ortholog Locus:PA4941
Sequence Length:870
Protein Length:289
Start: 5817176
Stop: 5816307
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08326.1|AE004907_4 (AE004907) protease subunit HflC [Pseudomonas aeruginosa PAO1]
Identities = 289/289 (100%), Positives = 289/289 (100%)
Sequence:
ATGGGCAACAAGTCTTTGATCGCTCTGATCGTCGGTGTCGTCGCCGCCATCGTCCTGTGGAACAGCGTCTAC
GTAGTCCAGCAGACCGAGCGCGCGGTCATGTTGCGCTTCGGCCGGGTGGTCGAGTCCGACGTCAAGCCGGGC
CTGCACTTCAAGATCCCGTACGTCAACCAGGTGCGCAAGTTCGACGCTCGCCTGCTGACCCTGGATGCCCCG
ACCCAGCGCTTCCTGACCCTGGAAAAGAAAGCGGTGATGGTGGATGCCTATGCCAAGTGGCGGGTGGCGGAT
GCCGAGCGCTTCTACACCGCTACCTCGGGCCTCAAGCAGATCGCCGACGAACGCCTGTCGCGGCGTCTCGAA
GCGGGTCTGCGCGACCAGTTCGGCAAGCGTACCCTGCATGAAGTGGTATCGGGCGAGCGCGACGCGCTGATG
GGCGACATCACCGCTTCGCTGAACCGCATGGCGCAGAAGGAGCTGGGTATCGAGGTGATCGACGTGCGGGTC
AAGGCCATCGATCTGCCGAAGGAAGTCAACCGCAGCGTCTTCGAGCGGATGAGCACCGAGCGTGAACGCGAG
GCCCGCGAGCACCGCGCCAAGGGTCGCGAACTGGCCGAAGGCATTCGTGCCGACGCCGATCGCCAGCGCCGG
GTAATCGTTGCCGAAGCCTACCGCGAGTCGGAAGAGACCCGCGGTGACGGCGACTCCAAGGCTGCCGCGATC
TATGCCAAGGCCTACAACCAGGATCCGGAGTTCTATTCCTTCTACCGTAGCCTGAAGGCGTATCGCGAAAGC
TTCGCCGAGAAGCGCGACGTACTGGTGCTCGATCCGAGCAGCGAGTTCTTCCGCTATCTGAACAAGGCCAAC
AAGTAA
Translation:
MGNKSLIALIVGVVAAIVLWNSVYVVQQTERAVMLRFGRVVESDVKPGLHFKIPYVNQVRKFDARLLTLDAP
TQRFLTLEKKAVMVDAYAKWRVADAERFYTATSGLKQIADERLSRRLEAGLRDQFGKRTLHEVVSGERDALM
GDITASLNRMAQKELGIEVIDVRVKAIDLPKEVNRSVFERMSTEREREAREHRAKGRELAEGIRADADRQRR
VIVAEAYRESEETRGDGDSKAAAIYAKAYNQDPEFYSFYRSLKAYRESFAEKRDVLVLDPSSEFFRYLNKAN
K*
AnnotationID:2788GeneID:2780
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 07:07:34
Gene Name:hflC
Confidence Code:2
GeneProduct:protease subunit HflC
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:integral membrane proteinase subunit
Functional Category Confidence Code:5
COGs:COG0330,pfam01145,smart00244
Secondary Functional Category(ies):cell division
EC Number:3.4.-.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58367.1| (AE016873) hflC protein [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 289

 Score =  494 bits (1272), Expect = e-139
 Identities = 247/287 (86%), Positives = 267/287 (93%)

>gb|AAN70458.1|AE016687_5 (AE016792) HflC protein [Pseudomonas putida KT2440]
          Length = 289

 Score =  488 bits (1257), Expect = e-137
 Identities = 243/287 (84%), Positives = 271/287 (94%)

>gb|AAN53684.1|AE015507_10 (AE015507) hflC protein [Shewanella oneidensis MR-1]
          Length = 297

 Score =  266 bits (681), Expect = 8e-71
 Identities = 140/297 (47%), Positives = 204/297 (68%), Gaps = 14/297 (4%)






 
Structural Features:
COG0330, HflC, Membrane protease subunits, stomatin/prohibitin homologs 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 291 residues,  96.9% aligned
Score =  172 bits (437), Expect = 4e-44
Identities = 88/288 (30%), Positives = 150/288 (52%), Gaps = 13/288 (4%)

pfam01145, Band_7, SPFH domain / Band 7 family.
CD-Length = 174 residues, 100.0% aligned
Score =  122 bits (307), Expect = 5e-29
Identities = 59/181 (32%), Positives = 96/181 (53%), Gaps = 7/181 (3%)

smart00244, PHB, prohibitin homologues
CD-Length = 160 residues, 100.0% aligned
Score =  103 bits (259), Expect = 2e-23
Identities = 57/165 (34%), Positives = 88/165 (53%), Gaps = 5/165 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5563




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22707




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35114




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2780_cdd.html


    		




 
URL:

















    ReferenceID:35903




  
Author/Investigator(s):
        Noble JA, Innis MA, Koonin EV, Rudd KE, Banuett F, Herskowitz I.





  
Title:
        The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain.




  
PubMed:
        8248183


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10866-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8248183

















    ReferenceID:35907




  
Author/Investigator(s):
        Banuett F, Herskowitz I.





  
Title:
        Identification of polypeptides encoded by an Escherichia coli locus (hflA) that governs the lysis-lysogeny decision of bacteriophage lambda.




  
PubMed:
        3040675


MedLine:
        




    
Source:
J Bacteriol. 1987 Sep;169(9):4076-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3040675









           
Homologs By Global Alignment 

           

       



     
Gene ID:2780
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4523
                           PA4941_tr
                           translation of PA4941
                           289
                           99.99
                           99.99
                           0
                           1440.0
     

     
     
130321
                           gb|AAG08326.1|AE004907_4
                           (AE004907) protease subunit HflC [Pseudomonas aeruginosa PAO1]
                           289
                           99.99
                           99.99
                           0
                           1440.0
     

     
     
130322
                           gb|AAO58367.1|
                           (AE016873) hflC protein [Pseudomonas syringae pv. tomato str. DC3000]
                           289
                           85.46
                           92.38
                           0
                           1271.0
     

     
     
130323
                           gb|AAN70458.1|AE016687_5
                           (AE016792) HflC protein [Pseudomonas putida KT2440]
                           289
                           84.08
                           93.77
                           0
                           1256.0
     

     
     
130325
                           gb|AAN53684.1|AE015507_10
                           (AE015507) hflC protein [Shewanella oneidensis MR-1]
                           301
                           47.17
                           68.43
                           16
                           693.0
     

     






      

          
GC ORFID: 45782How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4941Glimmer Score: 

          
Start: 5080857Stop: 5079988

          
Length: 870


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4941 translation of PA4941Homolog Bit Score 559.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3433 (ORF: BLASTX 496.0), t_PA4941 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGCAACAAGTCTTTGATCGCTCTGATCGTCGGTGTCGTCGCCGCCATCGTCCTGTGGAACAGCGTCTAC 
GTAGTCCAGCAGACCGAGCGCGCGGTCATGTTGCGCTTCGGCCGGGTGGTCGAGTCCGACGTCAAGCCGGGC 
CTGCACTTCAAGATCCCGTACGTCAACCAGGTGCGCAAGTTCGACGCTCGCCTGCTGACCCTGGATGCCCCG 
ACCCAGCGCTTCCTGACCCTGGAAAAGAAAGCGGTGATGGTGGATGCCTATGCCAAGTGGCGGGTGGCGGAT 
GCCGAGCGCTTCTACACCGCTACCTCGGGCCTCAAGCAGATCGCCGACGAACGCCTGTCGCGGCGTCTCGAA 
GCGGGTCTGCGCGACCAGTTCGGCAAGCGTACCCTGCATGAAGTGGTATCGGGCGAGCGCGACGCGCTGATG 
GGCGACATCACCGCTTCGCTGAACCGCATGGCGCAGAAGGAGCTGGGTATCGAGGTGATCGACGTGCGGGTC 
AAGGCCATCGATCTGCCGAAGGAAGTCAACCGCAGCGTCTTCGAGCGGATGAGCACCGAGCGTGAACGCGAG 
GCCCGCGAGCACCGCGCCAAGGGTCGCGAACTGGCCGAAGGCATTCGTGCCGACGCCGATCGCCAGCGCCGG 
GTAATCGTTGCCGAAGCCTACCGCGAGTCGGAAGAGACCCGCGGTGACGGCGACTCCAAGGCTGCCGCGATC 
TATGCCAAGGCCTACAACCAGGATCCGGAGTTCTATTCCTTCTACCGTAGCCTGAAGGCGTATCGCGAAAGC 
TTCGCCGAGAAGCGCGACGTACTGGTGCTCGATCCGAGCAGCGAGTTCTTCCGCTATCTGAACAAGGCCAAC 
AAGTAA