ViewGene
Locus Name:
GeneID:
Location:

GeneID:2777
TrivialName: PA14_17310
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17310
PAO1 Ortholog Locus:PA3636
Sequence Length:846
Protein Length:281
Start: 1487153
Stop: 1487998
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07024.1|AE004783_9 (AE004783) 2-dehydro-3-deoxyphosphooctonate aldolase [Pseudomonas aeruginosa PAO1]
Identities = 281/281 (100%), Positives = 281/281 (100%)
Sequence:
ATGGCACAGAAGATCGTTCGCGTCGGCGATATCCAGATCGGCAACGACCTGCCGTTCGTGCTGTTCGGCGGC
ATGAACGTGCTGGAGTCGCGCGACCTGGCCATGCAGGTCTGCGAAGAATACGTACGGGTTACCGAGAAACTC
GGTATCCCCTATGTGTTCAAGGCCAGCTTCGACAAGGCCAACCGTTCCTCGATCCACTCGTTCCGTGGCCCG
GGCCTGGAAGAGGGAATGAAGATCTTCGAAGAGATCAAGAAGACCTTCAAGGTGCCGGTCATCACCGATGTC
CACGAGCCGTTCCAGGCCCAGCCGGTGGCCGAGGTCTGCGACATCATCCAGCTGCCGGCCTTCCTCTCGCGG
CAGACCGACCTGGTGGTGGCGATGGCCAGGACCAACGCGGTGATCAATATCAAGAAGGCCCAGTTCCTCGCG
CCCCAGGAGATGAAGCACATCCTGACCAAGTGCGAGGAAGCCGGTAACGACCGCCTGATCCTCTGCGAGCGC
GGCTCCTCCTTCGGATACAACAACCTGGTGGTCGACATGCTCGGCTTCGGCATCATGAAGCAGTTCGAGTAC
CCGGTATTCTTCGACGTCACCCACGCCCTGCAGATGCCCGGCGGTCGCGCGGACTCCGCCGGCGGCCGGCGC
GCCCAGGTCACCGACCTGGCCAAGGCCGGGCTCAGCCAGAAGCTCGCCGGGCTGTTCCTCGAGGCCCATCCG
GACCCCGAGCACGCCAAGTGCGACGGTCCGTGCGCGCTGCGCCTGAACAAACTCGAAGCCTTCCTGTCCCAG
CTCAAGCAACTGGACGAGCTGATCAAGAGCTTTCCTGCAATCGAGACTGCCTAG
Translation:
MAQKIVRVGDIQIGNDLPFVLFGGMNVLESRDLAMQVCEEYVRVTEKLGIPYVFKASFDKANRSSIHSFRGP
GLEEGMKIFEEIKKTFKVPVITDVHEPFQAQPVAEVCDIIQLPAFLSRQTDLVVAMARTNAVINIKKAQFLA
PQEMKHILTKCEEAGNDRLILCERGSSFGYNNLVVDMLGFGIMKQFEYPVFFDVTHALQMPGGRADSAGGRR
AQVTDLAKAGLSQKLAGLFLEAHPDPEHAKCDGPCALRLNKLEAFLSQLKQLDELIKSFPAIETA*
AnnotationID:2785GeneID:2777
AnnotatorUID: diggins
Modification Date/Time: 2005-06-19 19:07:37
Gene Name:kdsA
Confidence Code:1
GeneProduct:2-dehydro-3-deoxyphosphooctonate aldolase
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase [Escherichia coli]
Functional Category Confidence Code:5
COGs:COG2877,pfam00793
Secondary Functional Category(ies):
EC Number:2.5.1.55 (formerly EC 4.1.2.16)
Status:ACTIVE
Pathway:
Lipopolysaccharide biosynthesis
Homology:
>gb|AAN67232.1|AE016350_5 (AE016779) 2-dehydro-3-deoxyphosphooctonate aldolase [Pseudomonas
           putida KT2440]
          Length = 281

 Score =  508 bits (1309), Expect = e-143
 Identities = 248/281 (88%), Positives = 269/281 (95%)

>gb|AAP16717.1| (AE016982) 2-dehydro-3-deoxyphosphooctulonate aldolase [Shigella
           flexneri 2a str. 2457T]
          Length = 284

 Score =  400 bits (1029), Expect = e-111
 Identities = 195/283 (68%), Positives = 239/283 (84%), Gaps = 2/283 (0%)

>gb|AAC43441.1| (U18555) 3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
           [Escherichia coli]
          Length = 284

 Score =  400 bits (1029), Expect = e-111
 Identities = 195/283 (68%), Positives = 239/283 (84%), Gaps = 2/283 (0%)
Structural Features:
COG2877, KdsA, 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 279 residues,  99.6% aligned
Score =  470 bits (1211), Expect = 2e-133
Identities = 179/278 (64%), Positives = 226/278 (81%)

pfam00793, DAHP_synth_1, DAHP synthetase I family.
CD-Length = 271 residues,  97.0% aligned
Score =  297 bits (761), Expect = 3e-81
Identities = 89/270 (32%), Positives = 132/270 (48%), Gaps = 12/270 (4%)
Genomic Context:






   
Comment:


















    ReferenceID:5557




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22701




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31907




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2777_cdd.html


    		




 
URL:

















    ReferenceID:34190




  
Author/Investigator(s):
        Walsh AG, Burrows LL, Lam JS.





  
Title:
        Genetic and biochemical characterization of an operon involved in the biosynthesis of 3-deoxy-D-manno-octulosonic acid in Pseudomonas aeruginosa.




  
PubMed:
        10220877


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 Apr 1;173(1):27-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10220877









           
Homologs By Global Alignment 

           

       



     
Gene ID:2777
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4517
                           PA3636_tr
                           translation of PA3636
                           281
                           99.99
                           99.99
                           0
                           1441.0
     

     
     
130207
                           gb|AAG07024.1|AE004783_9
                           (AE004783) 2-dehydro-3-deoxyphosphooctonate aldolase [Pseudomonas aeruginosa PAO1]
                           281
                           99.99
                           99.99
                           0
                           1441.0
     

     
     
130208
                           gb|AAD13217.1|
                           (AF098791) 2-dehydro-3-deoxyphosphooctonate aldolase [Pseudomonas aeruginosa]
                           281
                           99.99
                           99.99
                           0
                           1441.0
     

     
     
130209
                           gb|AAN67232.1|AE016350_5
                           (AE016779) 2-dehydro-3-deoxyphosphooctonate aldolase [Pseudomonas putida KT2440]
                           281
                           88.25
                           95.72
                           0
                           1309.0
     

     
     
130210
                           gb|AAO55073.1|
                           (AE016861) 2-dehydro-3-deoxyphosphooctonate aldolase [Pseudomonas syringae pv. tomato str. DC3000]
                           281
                           86.83
                           94.30
                           0
                           1286.0
     

     






      

          
GC ORFID: 45779How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3636Glimmer Score: 

          
Start: 3466784Stop: 3465939

          
Length: 846


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3636 translation of PA3636Homolog Bit Score 559.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4640 (ORF: BLASTX 500.0), t_PA3636 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCACAGAAGATCGTTCGCGTCGGCGATATCCAGATCGGCAACGACCTGCCGTTCGTGCTGTTCGGCGGC 
ATGAACGTGCTGGAGTCGCGCGACCTGGCCATGCAGGTCTGCGAAGAATACGTACGGGTTACCGAGAAACTC 
GGTATCCCCTATGTGTTCAAGGCCAGCTTCGACAAGGCCAACCGTTCCTCGATCCACTCGTTCCGTGGCCCG 
GGCCTGGAAGAGGGAATGAAGATCTTCGAAGAGATCAAGAAGACCTTCAAGGTGCCGGTCATCACCGATGTC 
CACGAGCCGTTCCAGGCCCAGCCGGTGGCCGAGGTCTGCGACATCATCCAGCTGCCGGCCTTCCTCTCGCGG 
CAGACCGACCTGGTGGTGGCGATGGCCAGGACCAACGCGGTGATCAATATCAAGAAGGCCCAGTTCCTCGCG 
CCCCAGGAGATGAAGCACATCCTGACCAAGTGCGAGGAAGCCGGTAACGACCGCCTGATCCTCTGCGAGCGC 
GGCTCCTCCTTCGGATACAACAACCTGGTGGTCGACATGCTCGGCTTCGGCATCATGAAGCAGTTCGAGTAC 
CCGGTATTCTTCGACGTCACCCACGCCCTGCAGATGCCCGGCGGTCGCGCGGACTCCGCCGGCGGCCGGCGC 
GCCCAGGTCACCGACCTGGCCAAGGCCGGGCTCAGCCAGAAGCTCGCCGGGCTGTTCCTCGAGGCCCATCCG 
GACCCCGAGCACGCCAAGTGCGACGGTCCGTGCGCGCTGCGCCTGAACAAACTCGAAGCCTTCCTGTCCCAG 
CTCAAGCAACTGGACGAGCTGATCAAGAGCTTTCCTGCAATCGAGACTGCCTAG