ViewGene
Locus Name:
GeneID:
Location:

GeneID:2774
TrivialName: PA14_03670
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03670
PAO1 Ortholog Locus:PA0281
Sequence Length:870
Protein Length:289
Start: 329955
Stop: 329086
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03670.1|AE004466_4 (AE004466) sulfate transport protein CysW [Pseudomonas aeruginosa PAO1]
Identities = 289/289 (100%), Positives = 289/289 (100%)
Sequence:
ATGTCCAGTGCAAGCATTTCCGCTGCCACCGCCAGCGCCTCCCGCCGCGGCAACGCCGGCGGCCGCCTGGCC
CTGATCGTCCTGGCCTGGCTGGTCTTCGCCCTGTTCCTCCTGCTGCCGCTCTACGTGGTCCTGAGCGAGGCG
CTGAAGCAGGGTTTCGGCACCTTCTTCGAGGCGATCCTCGAGCCCGACGCGCTGGCCGCGCTGAAGCTGACC
CTGATCGCGGTGGCCATCTCGGTGCCGCTCAATCTGCTGTTCGGCGTCGCCGCCGCCTGGTGCGTGAGCAAG
TTCGAGTTTCGCGGCAAGAGCATCCTGGTGACCCTGATCGACCTGCCGTTCTCGGTCTCGCCGGTGATCGCC
GGCCTGATCTACGTGCTGTTGTTCGGCGCCCAGGGCTACTTCGGCGAATGGCTGAGCGACCACGACATCCAG
ATCGTCTTCGCGGTGCCCGGCATCGTCCTGGCGACCCTCTTCGTCACCGTGCCCTTCGTCGCCCGCGAGCTG
ATCCCGCTGATGCAGGAACAGGGCACCCAGGAAGAGGAGGCCGCCCGCCTGCTCGGCGCCAACGGCTGGCAG
ATGTTCTGGCACGTGACCCTGCCGAACATTAAGTGGGGCCTGATCTACGGCGTGGTGCTCTGCACCGCCCGG
GCGATGGGCGAGTTCGGCGCGGTGTCGGTGGTTTCCGGACACATCCGCGGCGTCACCAACACCCTGCCGCTG
CACGTCGAGATCCTCTACAACGAATACAACCATGTCGCCGCCTTCAGCGTCGCCAGCCTGTTGCTGCTGATG
GCGCTGGTCATCCTTCTGCTCAAGCAGTGGAGCGAATCCCGCATGTCCCGACTGAAAAGCAATGCTGACGAG
GAATGA
Translation:
MSSASISAATASASRRGNAGGRLALIVLAWLVFALFLLLPLYVVLSEALKQGFGTFFEAILEPDALAALKLT
LIAVAISVPLNLLFGVAAAWCVSKFEFRGKSILVTLIDLPFSVSPVIAGLIYVLLFGAQGYFGEWLSDHDIQ
IVFAVPGIVLATLFVTVPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTLPNIKWGLIYGVVLCTAR
AMGEFGAVSVVSGHIRGVTNTLPLHVEILYNEYNHVAAFSVASLLLLMALVILLLKQWSESRMSRLKSNADE
E*
AnnotationID:2782GeneID:2774
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 05:05:10
Gene Name:cysW
Confidence Code:2
GeneProduct:sulfate transport protein CysW
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:sulfate ABC transporter, permease protein
Functional Category Confidence Code:5
COGs:COG4208
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70734.1|AE016717_2 (AE016793) sulfate ABC transporter, permease protein [Pseudomonas
           putida KT2440]
          Length = 290

 Score =  487 bits (1253), Expect = e-137
 Identities = 246/290 (84%), Positives = 270/290 (93%), Gaps = 1/290 (0%)

>gb|AAO53855.1| (AE016857) sulfate ABC transporter, permease protein CysW
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 290

 Score =  477 bits (1227), Expect = e-134
 Identities = 240/290 (82%), Positives = 267/290 (92%), Gaps = 1/290 (0%)

>emb|CAD84488.1| (BX321858) cysW; sulfate transport ABC transporter protein
           [Nitrosomonas europaea ATCC 19718]
          Length = 292

 Score =  380 bits (976), Expect = e-105
 Identities = 186/266 (69%), Positives = 226/266 (84%)






 
Structural Features:
COG4208, CysW, ABC-type sulfate transport system, permease component 
[Inorganic ion transport and metabolism]
CD-Length = 287 residues, 100.0% aligned
Score =  393 bits (1011), Expect = 1e-110
Identities = 182/287 (63%), Positives = 217/287 (75%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5551




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22695




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33633




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2774_cdd.html


    		




 
URL:

















    ReferenceID:35443




  
Author/Investigator(s):
        Laudenbach DE, Grossman AR.





  
Title:
        Characterization and mutagenesis of sulfur-regulated genes in a cyanobacterium: evidence for function in sulfate transport.




  
PubMed:
        1708375


MedLine:
        




    
Source:
J Bacteriol. 1991 May;173(9):2739-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1708375

















    ReferenceID:35446




  
Author/Investigator(s):
        Sirko A, Hryniewicz M, Hulanicka D, Bock A.





  
Title:
        Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster.




  
PubMed:
        2188958


MedLine:
        




    
Source:
J Bacteriol. 1990 Jun;172(6):3351-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2188958









           
Homologs By Global Alignment 

           

       



     
Gene ID:2774
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4514
                           PA0281_tr
                           translation of PA0281
                           289
                           99.99
                           99.99
                           0
                           1443.0
     

     
     
130142
                           gb|AAG03670.1|AE004466_4
                           (AE004466) sulfate transport protein CysW [Pseudomonas aeruginosa PAO1]
                           289
                           99.99
                           99.99
                           0
                           1443.0
     

     
     
130143
                           gb|AAN70734.1|AE016717_2
                           (AE016793) sulfate ABC transporter, permease protein [Pseudomonas putida KT2440]
                           290
                           84.82
                           93.10
                           1
                           1255.0
     

     
     
130144
                           gb|AAO53855.1|
                           (AE016857) sulfate ABC transporter, permease protein CysW [Pseudomonas syringae pv. tomato str. DC3000]
                           290
                           82.75
                           92.06
                           1
                           1229.0
     

     
     
130145
                           emb|CAD84488.1|
                           (BX321858) cysW; sulfate transport ABC transporter protein [Nitrosomonas europaea ATCC 19718]
                           292
                           64.72
                           80.13
                           3
                           971.0
     

     






      

          
GC ORFID: 45776How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0281Glimmer Score: 

          
Start: 6131296Stop: 6130427

          
Length: 870


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0281 translation of PA0281Homolog Bit Score 560.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4050 (ORF: BLASTX 477.0), t_PA0281 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCAGTGCAAGCATTTCCGCTGCCACCGCCAGCGCCTCCCGCCGCGGCAACGCCGGCGGCCGCCTGGCC 
CTGATCGTCCTGGCCTGGCTGGTCTTCGCCCTGTTCCTCCTGCTGCCGCTCTACGTGGTCCTGAGCGAGGCG 
CTGAAGCAGGGTTTCGGCACCTTCTTCGAGGCGATCCTCGAGCCCGACGCGCTGGCCGCGCTGAAGCTGACC 
CTGATCGCGGTGGCCATCTCGGTGCCGCTCAATCTGCTGTTCGGCGTCGCCGCCGCCTGGTGCGTGAGCAAG 
TTCGAGTTTCGCGGCAAGAGCATCCTGGTGACCCTGATCGACCTGCCGTTCTCGGTCTCGCCGGTGATCGCC 
GGCCTGATCTACGTGCTGTTGTTCGGCGCCCAGGGCTACTTCGGCGAATGGCTGAGCGACCACGACATCCAG 
ATCGTCTTCGCGGTGCCCGGCATCGTCCTGGCGACCCTCTTCGTCACCGTGCCCTTCGTCGCCCGCGAGCTG 
ATCCCGCTGATGCAGGAACAGGGCACCCAGGAAGAGGAGGCCGCCCGCCTGCTCGGCGCCAACGGCTGGCAG 
ATGTTCTGGCACGTGACCCTGCCGAACATTAAGTGGGGCCTGATCTACGGCGTGGTGCTCTGCACCGCCCGG 
GCGATGGGCGAGTTCGGCGCGGTGTCGGTGGTTTCCGGACACATCCGCGGCGTCACCAACACCCTGCCGCTG 
CACGTCGAGATCCTCTACAACGAATACAACCATGTCGCCGCCTTCAGCGTCGCCAGCCTGTTGCTGCTGATG 
GCGCTGGTCATCCTTCTGCTCAAGCAGTGGAGCGAATCCCGCATGTCCCGACTGAAAAGCAATGCTGACGAG 
GAATGA