ViewGene

Locus Name:
GeneID:
Location:

GeneID: 277

TrivialName: PA14_67630

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_67630

PAO1 Ortholog Locus: PA5121

Sequence Length: 2208

Protein Length: 735

Start: 6042902

Stop: 6040695

Strand: -

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG08506.1|AE004925_4 (AE004925) hypothetical protein [Pseudomonas aeruginosa PAO1] Identities = 735/735 (100%), Positives = 735/735 (100%)

Sequence:

GTGCCGATCATCGCCCGCTTCACCCTTCGCTCCCGCGTCCTGGCGGCCCTGCTGCTGTGCCTCTGCGCCGCC
CTGTTCTACTCGCCGCCGGGCGCCGCGGCGCTGCCGAACCCGTTGGCGGGGAGTTCCTCCGGTGGCGAGAAG
GTCAGCGATGCGCAGTTGGAACAATCGCTGAACCAGGTCATCCAGACCCTGGAGAACGACAGGCAGCGCGCC
GACCTGCTGAAAAAGCTGAAACAGCTGCGCGACGTGACCAAGAAAGGCAAGGAAAGCGAGGGTGGGGTGCTG
GGGCTGATCGGCGATACCCTGACCAGCCTGGAAAAGCAGTTCCAGGGCGCGAACAGCCCGGTCGCGTTATGG
GGCAACCAGTTCCAGCTGGCGCGTGCCGAACTCGAGGAGCGCTTTCCCAGCTGGCGCGACCTGCCGGCGATG
GTCTTCGATTTTTCGGTGGTGATCCTCCTCTGGGCCTGCCTGGCCAGCGCGCTGCTCTGGCTCAGCCGGCGC
ATGCGCGAGCGCTTCGGCCTGTCGGCGGAGTTGCCGCAGCATCCGAAGACCCGCGACCTGCTGCTGTTTTCC
CTGCGCAAGCTGGGCCCCTGGCTGGTGGCCTTCCTCATCACCCTGTACCTGTCGGTGGTGCTGCCGGACTCC
CTGAGCAAGACCCTGGCGATGGTCATGGCCTATGTGCTGGTCTGCGGCACGCTGTTCTCCGCGCTGTGCGTG
ATTTCCCTCTCGCTGCTCAGCGGCCCGCACCGCCAGCGCGCCCTGGATATCCTGCGGCGCCAGGCGTTCCGC
CCGTTGTGGCTGATCGGCAGCCTCGCCGCGCTGGGCGAGGTCGCCCACGATCCGCGGCTGATCGCCGGCCTC
GGCGAGCACACCTCGATCTGCCTGAGTACCCTGGCCAACGCCAGCGCCGCGCTGTTCACCGCGCTGTTCGTG
ATGCGTTTCCGCCGGCCGATCGCCCACCTGATCCGCAACCAGCCGCTGGAGCGCCGCCTCAAGCGCCGTAGC
CTGCACGACCTGGTGCAACTGGTCGGCTCGCTGTGGTTCGTGCCGGTGCTGGTGCTGGTGGGGATTTCCCTG
TTCGCCACCTTCGTTTCCGCCGGCGACAGCAGTTCGGCGTTGCGCCGGGCGCTGGTCTGCGCGGTGCTGGCG
GTGGTGGCGATGACCGTGATCGGGCTGATCCGCCGCCGTTCCAGTCGGGTCGGCGCCGGCCCGCGCCGCAGC
GCACCGTACATCGAGCAGTTGCAAAGCTTCGGCTACACCCTGCTGCACATCTTCGTGGTGCTGTTCTTCATC
GAGGTCGCCTTGCGCGTCTGGGGCATGTCGCTGATTCGCTATGCGGAAGGCGAGGGCGAGCAGATCAGCATG
AAGGTAGTCAGCTTCGGCACCACCCTGCTGGTCGCCTGGCTGATCTGGATCCTCACCGATACCGCGATCCAG
CACAGCCTCGGCCTGGGCGGCAAGAGCCGGCCGAACACCCGCGCGCTGACCATGCTGCCGCTGATCCGCAAC
GTGCTGTTCGCCACCATCGCGGTGATCGCCCTGATCGTCGCCCTGGCCAACATGGGCATGAACGTCACCCCG
CTGCTGGCCGGTGCCGGCGTGATCGGCCTGGCCATCGGCTTCGGTGCGCAGTCGCTGGTGGCGGACCTGATC
ACCGGCCTGTTCATCATCATCGAGGACTCGCTGTCGATCGACGACTACGTCGACGTCGGCGGCCACCTTGGC
ACGGTCGAGGGCCTGACCATCCGTACCGTGCGCCTGCGCGACCTGGACGGCGTGGTGCATACCATCCCGTTC
AGCGAGATCAAGAGCATCAAGAACTACTCGCGCCAGTTCGGCTACGCGATGTTCCGCTGGCCGGTGCCGGCG
AGCATGCCGATCGACGACGCGGTGGCGCTGGTGCGCGAGGTCGCGATGGAGCTGCGCAGCGACCCGGCGGTC
TACCGCAACCTGTGGTCGCCGATGGAATTGCAGGGCGTGGAGAGTTTCGACAACGGCCAGGCGATCCTGCGC
TTCCGCTTCAAGACCGCGCCGATCAAGCAGTGGGAGGTCCAGCGGGCGTTCAACCTGCGCCTGCGTCGGCGC
CTCGACCAGGCCGGCCTCGAACTGTCCATGCCGCGTCTCAACGTACAGTTGAGCCGGGCGCCCAAGGCGAAA
TCCGCGGAACCGGACGAGCAGGGCACGCCGGCGGACGGCGGCATCTAG

Translation:

MPIIARFTLRSRVLAALLLCLCAALFYSPPGAAALPNPLAGSSSGGEKVSDAQLEQSLNQVIQTLENDRQRA
DLLKKLKQLRDVTKKGKESEGGVLGLIGDTLTSLEKQFQGANSPVALWGNQFQLARAELEERFPSWRDLPAM
VFDFSVVILLWACLASALLWLSRRMRERFGLSAELPQHPKTRDLLLFSLRKLGPWLVAFLITLYLSVVLPDS
LSKTLAMVMAYVLVCGTLFSALCVISLSLLSGPHRQRALDILRRQAFRPLWLIGSLAALGEVAHDPRLIAGL
GEHTSICLSTLANASAALFTALFVMRFRRPIAHLIRNQPLERRLKRRSLHDLVQLVGSLWFVPVLVLVGISL
FATFVSAGDSSSALRRALVCAVLAVVAMTVIGLIRRRSSRVGAGPRRSAPYIEQLQSFGYTLLHIFVVLFFI
EVALRVWGMSLIRYAEGEGEQISMKVVSFGTTLLVAWLIWILTDTAIQHSLGLGGKSRPNTRALTMLPLIRN
VLFATIAVIALIVALANMGMNVTPLLAGAGVIGLAIGFGAQSLVADLITGLFIIIEDSLSIDDYVDVGGHLG
TVEGLTIRTVRLRDLDGVVHTIPFSEIKSIKNYSRQFGYAMFRWPVPASMPIDDAVALVREVAMELRSDPAV
YRNLWSPMELQGVESFDNGQAILRFRFKTAPIKQWEVQRAFNLRLRRRLDQAGLELSMPRLNVQLSRAPKAK
SAEPDEQGTPADGGI*

AnnotationID:287 GeneID:277

AnnotatorUID: diggins

Modification Date/Time: 2005-03-15 09:09:25

Gene Name:

Confidence Code: 3

GeneProduct: putative small-conductance mechanosensitive channel

Cell Localization: ()

Synonyms: PA5121

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (15) Membrane proteins

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: COG0668,pfam00924,COG3264

Secondary Functional Category(ies): cell envelope biogenesis, outer membrane

EC Number:

Status: ACTIVE

Pathway:

Homology:

Structural Features:

pfam00924, MS_channel, Mechanosensitive ion channel.
CD-Length = 205 residues,  99.5% aligned
Score =  149 bits (379), Expect = 8e-37
Identities = 64/205 (31%), Positives = 112/205 (54%), Gaps = 4/205 (1%)

COG0668, MscS, Small-conductance mechanosensitive channel 
[Cell envelope biogenesis, outer membrane]
CD-Length = 316 residues, 100.0% aligned
Score =  150 bits (379), Expect = 5e-37
Identities = 93/321 (28%), Positives = 164/321 (51%), Gaps = 14/321 (4%)

COG3264, COG3264, Small-conductance mechanosensitive channel 
[Cell envelope biogenesis, outer membrane]
CD-Length = 835 residues,  81.7% aligned
Score = 88.9 bits (220), Expect = 2e-18
Identities = 140/699 (20%), Positives = 258/699 (36%), Gaps = 57/699 (8%)

Genomic Context:

Comment:

ReferenceID:14099

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:17699

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:34900

Author/Investigator(s):

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: Misc (text/plain)

Data: 277_cdd.html

URL:

Homologs By Global Alignment

Gene ID:277

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

458 PA5121_tr translation of PA5121 735 99.99 99.99 0 3679.0

24288 gb|AAG08506.1|AE004925_4 (AE004925) hypothetical protein PA5121 [Pseudomonas aeruginosa PAO1] 735 99.86 99.99 0 3676.0

24289 gb|AAN67349.1|AE016361_3 (AE016780) conserved hypothetical protein [Pseudomonas putida KT2440] 743 60.43 74.56 34 2242.0

GC ORFID: `43279`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA5121`	Glimmer Score:
Start: `5306587`	Stop: `5304380`
Length: `2208`
Start Codon: `GTG`	Truncated Start:
Stop Codon: `TAG`	Truncated Stop:
Homolog: `t_PA5121 translation of PA5121`	Homolog Bit Score `1421.0`
Other Homologs: `t_PA5121 (ORF: GLIMMER)`
GC ORF Sequence
GTGCCGATCATCGCCCGCTTCACCCTTCGCTCCCGCGTCCTGGCGGCCCTGCTGCTGTGCCTCTGCGCCGCC CTGTTCTACTCGCCGCCGGGCGCCGCGGCGCTGCCGAACCCGTTGGCGGGGAGTTCCTCCGGTGGCGAGAAG GTCAGCGATGCGCAGTTGGAACAATCGCTGAACCAGGTCATCCAGACCCTGGAGAACGACAGGCAGCGCGCC GACCTGCTGAAAAAGCTGAAACAGCTGCGCGACGTGACCAAGAAAGGCAAGGAAAGCGAGGGTGGGGTGCTG GGGCTGATCGGCGATACCCTGACCAGCCTGGAAAAGCAGTTCCAGGGCGCGAACAGCCCGGTCGCGTTATGG GGCAACCAGTTCCAGCTGGCGCGTGCCGAACTCGAGGAGCGCTTTCCCAGCTGGCGCGACCTGCCGGCGATG GTCTTCGATTTTTCGGTGGTGATCCTCCTCTGGGCCTGCCTGGCCAGCGCGCTGCTCTGGCTCAGCCGGCGC ATGCGCGAGCGCTTCGGCCTGTCGGCGGAGTTGCCGCAGCATCCGAAGACCCGCGACCTGCTGCTGTTTTCC CTGCGCAAGCTGGGCCCCTGGCTGGTGGCCTTCCTCATCACCCTGTACCTGTCGGTGGTGCTGCCGGACTCC CTGAGCAAGACCCTGGCGATGGTCATGGCCTATGTGCTGGTCTGCGGCACGCTGTTCTCCGCGCTGTGCGTG ATTTCCCTCTCGCTGCTCAGCGGCCCGCACCGCCAGCGCGCCCTGGATATCCTGCGGCGCCAGGCGTTCCGC CCGTTGTGGCTGATCGGCAGCCTCGCCGCGCTGGGCGAGGTCGCCCACGATCCGCGGCTGATCGCCGGCCTC GGCGAGCACACCTCGATCTGCCTGAGTACCCTGGCCAACGCCAGCGCCGCGCTGTTCACCGCGCTGTTCGTG ATGCGTTTCCGCCGGCCGATCGCCCACCTGATCCGCAACCAGCCGCTGGAGCGCCGCCTCAAGCGCCGTAGC CTGCACGACCTGGTGCAACTGGTCGGCTCGCTGTGGTTCGTGCCGGTGCTGGTGCTGGTGGGGATTTCCCTG TTCGCCACCTTCGTTTCCGCCGGCGACAGCAGTTCGGCGTTGCGCCGGGCGCTGGTCTGCGCGGTGCTGGCG GTGGTGGCGATGACCGTGATCGGGCTGATCCGCCGCCGTTCCAGTCGGGTCGGCGCCGGCCCGCGCCGCAGC GCACCGTACATCGAGCAGTTGCAAAGCTTCGGCTACACCCTGCTGCACATCTTCGTGGTGCTGTTCTTCATC GAGGTCGCCTTGCGCGTCTGGGGCATGTCGCTGATTCGCTATGCGGAAGGCGAGGGCGAGCAGATCAGCATG AAGGTAGTCAGCTTCGGCACCACCCTGCTGGTCGCCTGGCTGATCTGGATCCTCACCGATACCGCGATCCAG CACAGCCTCGGCCTGGGCGGCAAGAGCCGGCCGAACACCCGCGCGCTGACCATGCTGCCGCTGATCCGCAAC GTGCTGTTCGCCACCATCGCGGTGATCGCCCTGATCGTCGCCCTGGCCAACATGGGCATGAACGTCACCCCG CTGCTGGCCGGTGCCGGCGTGATCGGCCTGGCCATCGGCTTCGGTGCGCAGTCGCTGGTGGCGGACCTGATC ACCGGCCTGTTCATCATCATCGAGGACTCGCTGTCGATCGACGACTACGTCGACGTCGGCGGCCACCTTGGC ACGGTCGAGGGCCTGACCATCCGTACCGTGCGCCTGCGCGACCTGGACGGCGTGGTGCATACCATCCCGTTC AGCGAGATCAAGAGCATCAAGAACTACTCGCGCCAGTTCGGCTACGCGATGTTCCGCTGGCCGGTGCCGGCG AGCATGCCGATCGACGACGCGGTGGCGCTGGTGCGCGAGGTCGCGATGGAGCTGCGCAGCGACCCGGCGGTC TACCGCAACCTGTGGTCGCCGATGGAATTGCAGGGCGTGGAGAGTTTCGACAACGGCCAGGCGATCCTGCGC TTCCGCTTCAAGACCGCGCCGATCAAGCAGTGGGAGGTCCAGCGGGCGTTCAACCTGCGCCTGCGTCGGCGC CTCGACCAGGCCGGCCTCGAACTGTCCATGCCGCGTCTCAACGTACAGTTGAGCCGGGCGCCCAAGGCGAAA TCCGCGGAACCGGACGAGCAGGGCACGCCGGCGGACGGCGGCATCTAG