ViewGene
Locus Name:
GeneID:
Location:

GeneID:2749
TrivialName: PA14_38350
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38350
PAO1 Ortholog Locus:PA2023
Sequence Length:840
Protein Length:279
Start: 3419098
Stop: 3418259
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05411.1|AE004628_9 (AE004628) UTP--glucose-1-phosphate uridylyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 279/279 (100%), Positives = 279/279 (100%)
Sequence:
ATGATCAAGAAATGTCTTTTCCCGGCCGCCGGTTACGGCACTCGTTTCCTCCCGGCCACCAAGGCCATGCCC
AAGGAAATGCTGCCGGTGGTGAACAAGCCGCTGATCCAGTACGCGGTGGAGGAGGCGCTGGAAGCCGGCCTT
TCCGAGATCGGCATCGTCACCGGCCGCGGCAAGCGTTCGCTGGAAGACCACTTCGACATCAGCTACGAGCTG
GAACACCAGATCCGCAACACCGACAAGGAAAAGTACCTGGTCGGCATCCGTCGGCTGATCGACGAGTGCACC
TTCGCCTACACCCGCCAGGTGGAGATGAAGGGCCTCGGCCACGCCATCCTCACCGGTCGTCCGCTGATCGGC
GACGAGCCGTTCGCCGTGGTCCTGGCCGACGACCTGTGCCTGAACCTCGAAGGCGACAGCGTGCTGAAGCAG
ATGGTCAAGCTGTACAACCAGTTCCGCTGCTCCATCGTGGCGATCCAGGAAGTGCCGCCGGAAGAGACCAAC
AAGTACGGCGTGATCGCCGGCGAGATGATCCGCGACGATATCTTCCGGGTGAACACCATGGTCGAGAAGCCG
AAGCCGGAAGAGGCGCCGTCGAACCTGGCGATCATCGGCCGCTACATCCTGACCCCGGACATCTTCGACCTG
ATCGAGCAGACCGAACCGGGCAAGGGCGGCGAGATCCAGATCACCGATGCCCTGATGAAGCAGGCCCAGGAC
GGCTGCGTGCTGGCCTACAAGTTCAAGGGCAAGCGTTTCGACTGCGGCAGCGCCGAGGGCTACATCGAGGCG
ACCAACTTCTGCTACGAAAACCTCTACAAGACCGGCAAGGCTCACTGA
Translation:
MIKKCLFPAAGYGTRFLPATKAMPKEMLPVVNKPLIQYAVEEALEAGLSEIGIVTGRGKRSLEDHFDISYEL
EHQIRNTDKEKYLVGIRRLIDECTFAYTRQVEMKGLGHAILTGRPLIGDEPFAVVLADDLCLNLEGDSVLKQ
MVKLYNQFRCSIVAIQEVPPEETNKYGVIAGEMIRDDIFRVNTMVEKPKPEEAPSNLAIIGRYILTPDIFDL
IEQTEPGKGGEIQITDALMKQAQDGCVLAYKFKGKRFDCGSAEGYIEATNFCYENLYKTGKAH*
AnnotationID:2757GeneID:2749
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 17:05:56
Gene Name:galU
Confidence Code:2
GeneProduct:UTP-glucose-1-phosphate uridylyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:UDP-glucose pyrophosphorylase
Functional Category Confidence Code:5
COGs:COG1210
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:2.7.7.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69415.1|AE016578_1 (AE016788) UTP-glucose-1-phosphate uridylyltransferase [Pseudomonas
           putida KT2440]
          Length = 279

 Score =  500 bits (1288), Expect = e-141
 Identities = 241/279 (86%), Positives = 263/279 (94%)

>gb|AAF93568.1| (AE004128) UTP--glucose-1-phosphate uridylyltransferase [Vibrio
           cholerae]
          Length = 291

 Score =  442 bits (1136), Expect = e-123
 Identities = 214/274 (78%), Positives = 244/274 (89%), Gaps = 1/274 (0%)

>gb|AAO36750.1| (AE015943) UTP-glucose-1-phosphate uridylyltransferase [Clostridium
           tetani E88]
          Length = 300

 Score =  279 bits (713), Expect = 1e-74
 Identities = 133/265 (50%), Positives = 193/265 (72%), Gaps = 4/265 (1%)






 
Structural Features:
COG1210, GalU, UDP-glucose pyrophosphorylase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 291 residues,  94.8% aligned
Score =  412 bits (1060), Expect = 3e-116
Identities = 156/278 (56%), Positives = 204/278 (73%), Gaps = 3/278 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5501




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22645




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32690




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2749_cdd.html


    		




 
URL:

















    ReferenceID:32691




  
Author/Investigator(s):
        Dean CR, Goldberg JB.





  
Title:
        Pseudomonas aeruginosa galU is required for a complete lipopolysaccharide core and repairs a secondary mutation in a PA103 (serogroup O11) wbpM mutant.




  
PubMed:
        12044687


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2002 May 7;210(2):277-83.






    
Reference Type:Online 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12044687

















    ReferenceID:32692




  
Author/Investigator(s):
        Chang HY, Huang HC, Lee JH, Peng HL.





  
Title:
        Characterization of a putative Pseudomonas UDPglucose pyrophosphorylase.




  
PubMed:
        10420655


MedLine:
        




    
Source:
Proc Natl Sci Counc Repub China B. 1999 Apr;23(2):74-84.






    
Reference Type:Online 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10420655

















    ReferenceID:32693




  
Author/Investigator(s):
        Genevaux P, Bauda P, DuBow MS, Oudega B.





  
Title:
        Identification of Tn10 insertions in the rfaG, rfaP, and galU genes involved in lipopolysaccharide core biosynthesis that affect Escherichia coli adhesion.




  
PubMed:
        10398745


MedLine:
        




    
Source:
Arch Microbiol. 1999 Jul;172(1):1-8.






    
Reference Type:Online 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10398745









           
Homologs By Global Alignment 

           

       



     
Gene ID:2749
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4483
                           PA2023_tr
                           translation of PA2023
                           279
                           99.99
                           99.99
                           0
                           1453.0
     

     
     
129671
                           gb|AAG05411.1|AE004628_9
                           (AE004628) UTP--glucose-1-phosphate uridylyltransferase [Pseudomonas aeruginosa PAO1]
                           279
                           99.99
                           99.99
                           0
                           1453.0
     

     
     
129672
                           emb|CAA09328.1|
                           (AJ010734) UDP-glucose pyrophosphorylase [Pseudomonas aeruginosa]
                           279
                           94.98
                           97.13
                           1
                           1373.0
     

     
     
129673
                           gb|AAN69415.1|AE016578_1
                           (AE016788) UTP-glucose-1-phosphate uridylyltransferase [Pseudomonas putida KT2440]
                           279
                           86.37
                           94.26
                           0
                           1288.0
     

     
     
129675
                           gb|AAO56600.1|
                           (AE016867) UTP-glucose-1-phosphate uridylyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           279
                           84.58
                           91.03
                           0
                           1240.0
     

     






      

          
GC ORFID: 45751How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2023Glimmer Score: 

          
Start: 1534812Stop: 1535651

          
Length: 840


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2023 translation of PA2023Homolog Bit Score 564.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7269 (ORF: BLASTX 481.0), t_PA2023 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCAAGAAATGTCTTTTCCCGGCCGCCGGTTACGGCACTCGTTTCCTCCCGGCCACCAAGGCCATGCCC 
AAGGAAATGCTGCCGGTGGTGAACAAGCCGCTGATCCAGTACGCGGTGGAGGAGGCGCTGGAAGCCGGCCTT 
TCCGAGATCGGCATCGTCACCGGCCGCGGCAAGCGTTCGCTGGAAGACCACTTCGACATCAGCTACGAGCTG 
GAACACCAGATCCGCAACACCGACAAGGAAAAGTACCTGGTCGGCATCCGTCGGCTGATCGACGAGTGCACC 
TTCGCCTACACCCGCCAGGTGGAGATGAAGGGCCTCGGCCACGCCATCCTCACCGGTCGTCCGCTGATCGGC 
GACGAGCCGTTCGCCGTGGTCCTGGCCGACGACCTGTGCCTGAACCTCGAAGGCGACAGCGTGCTGAAGCAG 
ATGGTCAAGCTGTACAACCAGTTCCGCTGCTCCATCGTGGCGATCCAGGAAGTGCCGCCGGAAGAGACCAAC 
AAGTACGGCGTGATCGCCGGCGAGATGATCCGCGACGATATCTTCCGGGTGAACACCATGGTCGAGAAGCCG 
AAGCCGGAAGAGGCGCCGTCGAACCTGGCGATCATCGGCCGCTACATCCTGACCCCGGACATCTTCGACCTG 
ATCGAGCAGACCGAACCGGGCAAGGGCGGCGAGATCCAGATCACCGATGCCCTGATGAAGCAGGCCCAGGAC 
GGCTGCGTGCTGGCCTACAAGTTCAAGGGCAAGCGTTTCGACTGCGGCAGCGCCGAGGGCTACATCGAGGCG 
ACCAACTTCTGCTACGAAAACCTCTACAAGACCGGCAAGGCTCACTGA