ViewGene
Locus Name:
GeneID:
Location:

GeneID:2746
TrivialName: PA14_02560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02560
PAO1 Ortholog Locus:PA0209
Sequence Length:882
Protein Length:293
Start: 229047
Stop: 229928
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03598.1|AE004459_2 (AE004459) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 290/293 (98%), Positives = 290/293 (98%)
Sequence:
ATGAACGCCATCGCGAACCTCGCGGCGACGCTGCGCGCCGACCTCGGCGAATGCCTGGCCGACCTGGCGGTG
GACGCCCTCATCGACGAAGCCGAACTGTCGCCCAAACCGGCCCTGGTGGACCGTCGCGGCAATGGCGCGCAT
GCCGACCTGCACCTGGGCCTGATGCAGGCCTCGGCGCTGAGCCTGTGGCCGTGTTTCAAGGAAATGGCCGAC
GCCGCCCAGCGGCATGGGCGCATCGATGCGCGCCTGCGCGGCGTCCTTGGCCAACTGGGGCGCGACGGCGAG
GCGGCGATGCTGCGTACCACCGAAGGCGTGAACACCCATCGCGGAGCGATCTGGGCGCTCGGCCTGCTGGTC
GCGGCCGCCGCTCTCGAACCGCGCCGCACGCAGGCCGGCGAAGTGGCCGCGCGCGCAGGACGCATCGCCCTG
CTCGACGATCCGGCGGCCGCCATCGGCGACAGCCACGGCGAGCGCGTCCGTCGGCGCTACGGCGTCGGCGGC
GCCCGCGAGGAGGCGCGTCTCGGCTTCCCCCGCGCAGTGCGCCACGGCCTGCCGCAGCTATGGCGCAGCCGC
GAAGGCGGCGCTGGCGAGCAGAACGCCAGGCTCGATGCGCTGCTGGCGATCATGAGCGTCCTCGACGATACC
TGCGTGCTGCACCGCGCCGGCCGCGTCGGCCTCGCCGCGATGCAGGATGGCGCCCGCGCGGTGCTCGCCGCC
GGCGGCAGCGCCAGTCTCGCCGGGCGTCGCCGCCTGTGCGAGCTGGACCGCCGCCTGCTGGCGCTGAACGCT
TCGCCCGGCGGCGCCGCCGACCTGCTGGCCGCCTGTCTTTTCCTCGATCGCCTGCCCGCCGTGTCCGGTGGG
TGGGCTGGGAGTCTCTGA
Translation:
MNAIANLAATLRADLGECLADLAVDALIDEAELSPKPALVDRRGNGAHADLHLGLMQASALSLWPCFKEMAD
AAQRHGRIDARLRGVLGQLGRDGEAAMLRTTEGVNTHRGAIWALGLLVAAAALEPRRTQAGEVAARAGRIAL
LDDPAAAIGDSHGERVRRRYGVGGAREEARLGFPRAVRHGLPQLWRSREGGAGEQNARLDALLAIMSVLDDT
CVLHRAGRVGLAAMQDGARAVLAAGGSASLAGRRRLCELDRRLLALNASPGGAADLLAACLFLDRLPAVSGG
WAGSL*
AnnotationID:2754GeneID:2746
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 12:12:04
Gene Name:
Confidence Code:3
GeneProduct:putative Triphosphoribosyl-dephospho-CoA synthetase
Cell Localization:()
Synonyms:mdcB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1767,pfam01874
Secondary Functional Category(ies):
EC Number:2.7.8.25
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA36205.1| (AB017138) mdcB [Pseudomonas putida]
          Length = 301

 Score =  358 bits (920), Expect = 2e-98
 Identities = 190/274 (69%), Positives = 215/274 (78%), Gaps = 1/274 (0%)

gb|AAO58513.1| (AE016874) 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A
           synthase [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 291

 Score =  350 bits (898), Expect = 6e-96
 Identities = 188/275 (68%), Positives = 210/275 (76%)

>gb|AAC45454.1| (U95087) MdcB [Klebsiella pneumoniae]
          Length = 280

 Score =  269 bits (687), Expect = 2e-71
 Identities = 156/264 (59%), Positives = 177/264 (67%), Gaps = 1/264 (0%)








 
Structural Features:
COG1767, CitG, Triphosphoribosyl-dephospho-CoA synthetase 
[Coenzyme metabolism]
CD-Length = 288 residues,  99.3% aligned
Score =  200 bits (509), Expect = 2e-52
Identities = 109/286 (38%), Positives = 146/286 (51%), Gaps = 12/286 (4%)

pfam01874, CitG, ATP:dephospho-CoA triphosphoribosyl transferase.
CD-Length = 274 residues,  99.6% aligned
Score =  177 bits (449), Expect = 2e-45
Identities = 98/274 (35%), Positives = 135/274 (49%), Gaps = 23/274 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5495




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22639




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33574




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2746_cdd.html


    		




 
URL:

















    ReferenceID:35467




  
Author/Investigator(s):
        Chohnan S, Kurusu Y, Nishihara H, Takamura Y.





  
Title:
        Cloning and characterization of mdc genes encoding malonate decarboxylase from Pseudomonas putida.




  
PubMed:
        10339824


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1999 May 15;174(2):311-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10339824

















    ReferenceID:35471




  
Author/Investigator(s):
        Koo JH, Jung SB, Byun HS, Kim YS.





  
Title:
        Cloning and sequencing of genes encoding malonate decarboxylase in Acinetobacter calcoaceticus.




  
PubMed:
        9375791


MedLine:
        




    
Source:
Biochim Biophys Acta. 1997 Oct 9;1354(1):49-54.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9375791









           
Homologs By Global Alignment 

           

       



     
Gene ID:2746
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4480
                           PA0209_tr
                           translation of PA0209
                           293
                           98.97
                           98.97
                           0
                           1455.0
     

     
     
129650
                           gb|AAG03598.1|AE004459_2
                           (AE004459) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           293
                           98.97
                           98.97
                           0
                           1455.0
     

     
     
129651
                           dbj|BAA36205.1|
                           (AB017138) mdcB [Pseudomonas putida]
                           311
                           64.63
                           72.99
                           28
                           921.0
     

     
     
129652
                           gb|AAO58513.1|
                           (AE016874) 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           297
                           64.30
                           72.72
                           10
                           892.0
     

     
     
129653
                           gb|AAC45454.1|
                           (U95087) MdcB [Klebsiella pneumoniae]
                           293
                           54.60
                           62.45
                           13
                           687.0
     

     






      

          
GC ORFID: 45748How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0209Glimmer Score: 

          
Start: 6030388Stop: 6031269

          
Length: 882


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0209 translation of PA0209Homolog Bit Score 565.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3318 (ORF: BLASTX 353.0), t_PA0209 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGCCATCGCGAACCTCGCGGCGACGCTGCGCGCCGACCTCGGCGAATGCCTGGCCGACCTGGCGGTG 
GACGCCCTCATCGACGAAGCCGAACTGTCGCCCAAACCGGCCCTGGTGGACCGTCGCGGCAATGGCGCGCAT 
GCCGACCTGCACCTGGGCCTGATGCAGGCCTCGGCGCTGAGCCTGTGGCCGTGTTTCAAGGAAATGGCCGAC 
GCCGCCCAGCGGCATGGGCGCATCGATGCGCGCCTGCGCGGCGTCCTTGGCCAACTGGGGCGCGACGGCGAG 
GCGGCGATGCTGCGTACCACCGAAGGCGTGAACACCCATCGCGGAGCGATCTGGGCGCTCGGCCTGCTGGTC 
GCGGCCGCCGCTCTCGAACCGCGCCGCACGCAGGCCGGCGAAGTGGCCGCGCGCGCAGGACGCATCGCCCTG 
CTCGACGATCCGGCGGCCGCCATCGGCGACAGCCACGGCGAGCGCGTCCGTCGGCGCTACGGCGTCGGCGGC 
GCCCGCGAGGAGGCGCGTCTCGGCTTCCCCCGCGCAGTGCGCCACGGCCTGCCGCAGCTATGGCGCAGCCGC 
GAAGGCGGCGCTGGCGAGCAGAACGCCAGGCTCGATGCGCTGCTGGCGATCATGAGCGTCCTCGACGATACC 
TGCGTGCTGCACCGCGCCGGCCGCGTCGGCCTCGCCGCGATGCAGGATGGCGCCCGCGCGGTGCTCGCCGCC 
GGCGGCAGCGCCAGTCTCGCCGGGCGTCGCCGCCTGTGCGAGCTGGACCGCCGCCTGCTGGCGCTGAACGCT 
TCGCCCGGCGGCGCCGCCGACCTGCTGGCCGCCTGTCTTTTCCTCGATCGCCTGCCCGCCGTGTCCGGTGGG 
TGGGCTGGGAGTCTCTGA