ViewGene
Locus Name:
GeneID:
Location:

GeneID:274
TrivialName: PA14_71870
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71870
PAO1 Ortholog Locus:PA5443
Sequence Length:2187
Protein Length:728
Start: 6404008
Stop: 6406194
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08828.1|AE004957_3 (AE004957) DNA helicase II [Pseudomonas aeruginosa PAO1]
Identities = 723/728 (99%), Positives = 728/728 (100%)
Sequence:
ATGAACGACGACCTCTCCCTCCTGCTGAACTCCCTCAACGACCCGCAGCGCCAGGCCGTGGCCGCGCCGCTG
GGCCGCCAACTGGTCCTCGCCGGCGCCGGCTCGGGCAAGACCCGCGTGCTGGTGCACCGGATCGCCTGGCTG
ATCCAGGTCGAGCACGCCTCGCCCTACAGCATCCTGTCGGTGACCTTCACCAACAAGGCGGCGGCGGAGATG
CGCCACCGCATCGAGCAGTTGCTCGGGATCAATCCGGCGGGCATGTGGGTCGGCACCTTCCACGGCCTCGCC
CATCGCCTGCTGCGCGCGCACTGGCGCGAGGCCGGGCTGAGCGAGAACTTCCAGATCCTCGACAGCGACGAC
CAGCAACGCCTGGTCAAGCGGGTGATCCGCGAACTCGGCCTCGACGAGCAGCGCTGGCCGGCGCGCCAGGCC
CAGTGGTTCATCAACGGGCAGAAGGACGAGGGCCTGCGGCCGCAACACATCCAGCCCGGCGGCGACCTGTTC
CTCGCCACCATGCTGAAGATCTACGAAGCCTACGAGGCGGCCTGCGCCCGCGCCGGGGTGATCGACTTCTCC
GAGCTGCTGCTGCGCGCCCTGGACCTCTGGCGCGACCATCCCGGCGTGCTCGAGCACTACCAGCGGCGCTTC
CGCCATATCCTGGTGGACGAGTTCCAGGACACCAACGCCGTGCAGTACGCCTGGCTGCGGATTCTCGCCAAG
GGCGGCGACAGCCTGATGGTGGTCGGCGACGACGACCAGTCGATCTACGGCTGGCGTGGCGCACGGATCGAG
AACATCCAACAGTTCAGCGACGACTTCGCCGACGCCGAGGTGATCCGCCTGGAGCAGAACTACCGTTCCACC
GCGTCGATCCTCAAGGCCGCCAACGCCCTGATCGCCAACAACCAGGGACGCCTGGGCAAGGAGTTGTGGACC
GACGGCGAGGACGGCGAGTCGCTGAGCCTGTACGCCGCGTTCAACGAGCACGACGAGGCGCGCTACGTGGTC
GAGTCGATCGAGAGCGCGCTCAGAGGCGGCCTGGCGCGCAGCGAGATCGCCATCCTCTACCGCTCCAACGCC
CAGTCGCGGGTGCTGGAGGAAGCCCTGCTGCGGGAGAAGATTCCCTACCGCATCTACGGCGGCCAGCGCTTC
TTCGAGCGTGCCGAGATCAAGAACGCCATGGCCTACCTGCGCCTGCTCGACGGCCGCGGCAACGACGCGGCG
CTGGAACGGGTGGTCAACGTGCCGGCACGCGGCATCGGCGAGAAGACCGTGGAGAGCATCCGCGAATTCGCC
CGCGGCAACGATGTGTCGATGTGGGAGGCGATTCGCCTGATGATCGCCAACAAGGTCCTGCCCGGCCGCGCC
GCCAGCGCCCTGACCGGCTTCGTCGAGCTGATCGAGAATCTTTCGGCGAAGGTCATGGACATGCCGCTGCAC
CTGATGACCCAGACGGTGATCGAGCAGTCCGGACTGATCAGCTACCACAAGGAAGAAAAAGGCGAGAAAGGC
CAGGCCCGGGTGGAGAACCTCGAGGAACTGGTCAGCGCCGCCCGCGCCTTCGAGAACAGCGAGGAAGAGGAA
GACCTCACCCCGCTGCAGGCCTTCCTCAGCCACGCCTCCCTGGAGGCCGGGGAAACCCAGGCCGACGCCCAC
GAGGACAGTATCCAACTGATGACCCTGCACAGCGCCAAGGGCCTGGAGTTCCCGCTGGTGTTCCTGGTCGGC
ATGGAGGAAGGGCTGTTCCCGCATAAGATGAGCCTGGAGGAATCCGGCCGCCTGGAAGAGGAACGCCGCCTG
GCCTACGTCGGCGTCACCCGCGCCATGCAGCGACTGGTCCTGACCTACGCGGAGACCCGCCGGCTCTACGGT
AGCGAGACCTACAACAAGGTTTCGCGCTTCATCCGCGAGATCCCGCCGTCGCTGATCCAGGAAGTGCGCCTG
TCCAATACCGTCAGCCGCCCCTACGGCGGCACTTCGCGCAACGCCGGCGGCAGCCTGTTCAGCGGCGCCGGG
GTGCCGGAGACGCCCTTCTCCCTCGGCCAGCGGGTGCGCCACGCGCTGTTCGGCGAAGGGACTATCCTCAAC
TTCGAAGGCGCCGGCGCCCAGGCCCGGGTGCAGGTGAACTTCGAGAGCGAAGGCAGCAAGTGGCTGATGCTC
GGCTACGCCAAGCTGGAAGCCCTGTAG
Translation:
MNDDLSLLLNSLNDPQRQAVAAPLGRQLVLAGAGSGKTRVLVHRIAWLIQVEHASPYSILSVTFTNKAAAEM
RHRIEQLLGINPAGMWVGTFHGLAHRLLRAHWREAGLSENFQILDSDDQQRLVKRVIRELGLDEQRWPARQA
QWFINGQKDEGLRPQHIQPGGDLFLATMLKIYEAYEAACARAGVIDFSELLLRALDLWRDHPGVLEHYQRRF
RHILVDEFQDTNAVQYAWLRILAKGGDSLMVVGDDDQSIYGWRGARIENIQQFSDDFADAEVIRLEQNYRST
ASILKAANALIANNQGRLGKELWTDGEDGESLSLYAAFNEHDEARYVVESIESALRGGLARSEIAILYRSNA
QSRVLEEALLREKIPYRIYGGQRFFERAEIKNAMAYLRLLDGRGNDAALERVVNVPARGIGEKTVESIREFA
RGNDVSMWEAIRLMIANKVLPGRAASALTGFVELIENLSAKVMDMPLHLMTQTVIEQSGLISYHKEEKGEKG
QARVENLEELVSAARAFENSEEEEDLTPLQAFLSHASLEAGETQADAHEDSIQLMTLHSAKGLEFPLVFLVG
MEEGLFPHKMSLEESGRLEEERRLAYVGVTRAMQRLVLTYAETRRLYGSETYNKVSRFIREIPPSLIQEVRL
SNTVSRPYGGTSRNAGGSLFSGAGVPETPFSLGQRVRHALFGEGTILNFEGAGAQARVQVNFESEGSKWLML
GYAKLEAL*
AnnotationID:284GeneID:274
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 17:05:23
Gene Name:uvrD
Confidence Code:2
GeneProduct:DNA helicase II
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0210,pfam00580
Secondary Functional Category(ies):
EC Number:3.6.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58935.1| (AE016876) DNA helicase II [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 727

 Score = 1245 bits (3222), Expect = 0.0
 Identities = 621/728 (85%), Positives = 680/728 (93%), Gaps = 1/728 (0%)

>gb|AAN70917.1|AE016735_10 (AE016794) DNA helicase II [Pseudomonas putida KT2440]
          Length = 728

 Score = 1213 bits (3139), Expect = 0.0
 Identities = 606/726 (83%), Positives = 667/726 (91%), Gaps = 1/726 (0%)

>gb|AAC46278.1| (AF028736) DNA helicase II [Serratia marcescens]
          Length = 720

 Score =  945 bits (2443), Expect = 0.0
 Identities = 466/725 (64%), Positives = 582/725 (80%), Gaps = 6/725 (0%)








 
Structural Features:
COG0210, UvrD, Superfamily I DNA and RNA helicases 
[DNA replication, recombination, and repair]
CD-Length = 655 residues,  99.7% aligned
Score =  619 bits (1598), Expect = 2e-178
Identities = 304/658 (46%), Positives = 409/658 (62%), Gaps = 19/658 (2%)

pfam00580, UvrD-helicase, UvrD/REP helicase.
CD-Length = 496 residues, 100.0% aligned
Score =  468 bits (1205), Expect = 1e-132
Identities = 215/496 (43%), Positives = 304/496 (61%), Gaps = 15/496 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:551




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17693




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34668




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:274_cdd.html


    		




 
URL:

















    ReferenceID:35629




  
Author/Investigator(s):
        Rocchetta HL, Burrows LL, Pacan JC, Lam JS.





  
Title:
        Three rhamnosyltransferases responsible for assembly of the A-band D-rhamnan polysaccharide in Pseudomonas aeruginosa: a fourth transferase, WbpL, is required for the initiation of both A-band and B-band lipopolysaccharide synthesis.




  
PubMed:
        9680202


MedLine:
        




    
Source:
Mol Microbiol. 1998 Jun;28(6):1103-19.

Erratum in:
    * Mol Microbiol 1998 Dec;30(5):1131.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9680202









           
Homologs By Global Alignment 

           

       



     
Gene ID:274
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
455
                           PA5443_tr
                           translation of PA5443
                           728
                           99.31
                           99.99
                           0
                           3684.0
     

     
     
24083
                           gb|AAG08828.1|AE004957_3
                           (AE004957) DNA helicase II [Pseudomonas aeruginosa PAO1]
                           728
                           99.31
                           99.99
                           0
                           3684.0
     

     
     
24084
                           gb|AAF42852.1|
                           (AF220057) mismatch repair protein MutU [Pseudomonas aeruginosa]
                           728
                           99.31
                           99.99
                           0
                           3684.0
     

     
     
24085
                           gb|AAO58935.1|
                           (AE016876) DNA helicase II [Pseudomonas syringae pv. tomato str. DC3000]
                           728
                           85.30
                           93.40
                           1
                           3224.0
     

     
     
24086
                           gb|AAN70917.1|AE016735_10
                           (AE016794) DNA helicase II [Pseudomonas putida KT2440]
                           729
                           83.12
                           91.49
                           2
                           3140.0
     

     






      

          
GC ORFID: 43276How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5443Glimmer Score: 

          
Start: 5667697Stop: 5669883

          
Length: 2187


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5443 translation of PA5443Homolog Bit Score 1423.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2918 (ORF: BLASTX 1243.0), t_PA5443 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGACGACCTCTCCCTCCTGCTGAACTCCCTCAACGACCCGCAGCGCCAGGCCGTGGCCGCGCCGCTG 
GGCCGCCAACTGGTCCTCGCCGGCGCCGGCTCGGGCAAGACCCGCGTGCTGGTGCACCGGATCGCCTGGCTG 
ATCCAGGTCGAGCACGCCTCGCCCTACAGCATCCTGTCGGTGACCTTCACCAACAAGGCGGCGGCGGAGATG 
CGCCACCGCATCGAGCAGTTGCTCGGGATCAATCCGGCGGGCATGTGGGTCGGCACCTTCCACGGCCTCGCC 
CATCGCCTGCTGCGCGCGCACTGGCGCGAGGCCGGGCTGAGCGAGAACTTCCAGATCCTCGACAGCGACGAC 
CAGCAACGCCTGGTCAAGCGGGTGATCCGCGAACTCGGCCTCGACGAGCAGCGCTGGCCGGCGCGCCAGGCC 
CAGTGGTTCATCAACGGGCAGAAGGACGAGGGCCTGCGGCCGCAACACATCCAGCCCGGCGGCGACCTGTTC 
CTCGCCACCATGCTGAAGATCTACGAAGCCTACGAGGCGGCCTGCGCCCGCGCCGGGGTGATCGACTTCTCC 
GAGCTGCTGCTGCGCGCCCTGGACCTCTGGCGCGACCATCCCGGCGTGCTCGAGCACTACCAGCGGCGCTTC 
CGCCATATCCTGGTGGACGAGTTCCAGGACACCAACGCCGTGCAGTACGCCTGGCTGCGGATTCTCGCCAAG 
GGCGGCGACAGCCTGATGGTGGTCGGCGACGACGACCAGTCGATCTACGGCTGGCGTGGCGCACGGATCGAG 
AACATCCAACAGTTCAGCGACGACTTCGCCGACGCCGAGGTGATCCGCCTGGAGCAGAACTACCGTTCCACC 
GCGTCGATCCTCAAGGCCGCCAACGCCCTGATCGCCAACAACCAGGGACGCCTGGGCAAGGAGTTGTGGACC 
GACGGCGAGGACGGCGAGTCGCTGAGCCTGTACGCCGCGTTCAACGAGCACGACGAGGCGCGCTACGTGGTC 
GAGTCGATCGAGAGCGCGCTCAGAGGCGGCCTGGCGCGCAGCGAGATCGCCATCCTCTACCGCTCCAACGCC 
CAGTCGCGGGTGCTGGAGGAAGCCCTGCTGCGGGAGAAGATTCCCTACCGCATCTACGGCGGCCAGCGCTTC 
TTCGAGCGTGCCGAGATCAAGAACGCCATGGCCTACCTGCGCCTGCTCGACGGCCGCGGCAACGACGCGGCG 
CTGGAACGGGTGGTCAACGTGCCGGCACGCGGCATCGGCGAGAAGACCGTGGAGAGCATCCGCGAATTCGCC 
CGCGGCAACGATGTGTCGATGTGGGAGGCGATTCGCCTGATGATCGCCAACAAGGTCCTGCCCGGCCGCGCC 
GCCAGCGCCCTGACCGGCTTCGTCGAGCTGATCGAGAATCTTTCGGCGAAGGTCATGGACATGCCGCTGCAC 
CTGATGACCCAGACGGTGATCGAGCAGTCCGGACTGATCAGCTACCACAAGGAAGAAAAAGGCGAGAAAGGC 
CAGGCCCGGGTGGAGAACCTCGAGGAACTGGTCAGCGCCGCCCGCGCCTTCGAGAACAGCGAGGAAGAGGAA 
GACCTCACCCCGCTGCAGGCCTTCCTCAGCCACGCCTCCCTGGAGGCCGGGGAAACCCAGGCCGACGCCCAC 
GAGGACAGTATCCAACTGATGACCCTGCACAGCGCCAAGGGCCTGGAGTTCCCGCTGGTGTTCCTGGTCGGC 
ATGGAGGAAGGGCTGTTCCCGCATAAGATGAGCCTGGAGGAATCCGGCCGCCTGGAAGAGGAACGCCGCCTG 
GCCTACGTCGGCGTCACCCGCGCCATGCAGCGACTGGTCCTGACCTACGCGGAGACCCGCCGGCTCTACGGT 
AGCGAGACCTACAACAAGGTTTCGCGCTTCATCCGCGAGATCCCGCCGTCGCTGATCCAGGAAGTGCGCCTG 
TCCAATACCGTCAGCCGCCCCTACGGCGGCACTTCGCGCAACGCCGGCGGCAGCCTGTTCAGCGGCGCCGGG 
GTGCCGGAGACGCCCTTCTCCCTCGGCCAGCGGGTGCGCCACGCGCTGTTCGGCGAAGGGACTATCCTCAAC 
TTCGAAGGCGCCGGCGCCCAGGCCCGGGTGCAGGTGAACTTCGAGAGCGAAGGCAGCAAGTGGCTGATGCTC 
GGCTACGCCAAGCTGGAAGCCCTGTAG