ViewGene
Locus Name:
GeneID:
Location:

GeneID:2739
TrivialName: PA14_41350
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41350
PAO1 Ortholog Locus:PA1796
Sequence Length:855
Protein Length:284
Start: 3687635
Stop: 3688489
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05185.1|AE004605_7 (AE004605) 5,10-methylene-tetrahydrofolate dehydrogenase / cyclohydrolase [Pseudomonas aeruginosa PAO1]
Identities = 284/284 (100%), Positives = 284/284 (100%)
Sequence:
ATGACCGCACAACTGATCGACGGCAAAGCGATCGCCGCCAACCTTCGCCAGCAGATAGCCCAACGCGTGACC
GAGCGCCGCCAGCAAGGCCTGCGCGTTCCCGGCCTGGCGGTGATCCTGGTCGGCACCGATCCGGCCTCTCAG
GTCTATGTGGCGCACAAGCGCAAGGACTGCGAGGAAGTCGGCTTTCTCTCCCAGGCCTACGATCTTCCCGCC
GAAACCAGCCAGGACGACCTGCTGGCCCTGATCGACCGCCTGAACGACGATCCCGCCATCGACGGCATCCTG
GTCCAGCTACCCCTGCCCGCCCACCTGGACGCCTCCCTGCTGCTGGAGCGTATCCACCCGGACAAGGACGTG
GACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAA
GGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTCG
AACATCGTCGGCCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCACCGTCACCGTGACCCACCGCTTC
ACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTC
AAGGGCGAGTGGATCAAGGAAGGCGCCATCGTCATCGACGTCGGCATCAACCGCCAGGCCGACGGCCGCCTG
GTCGGCGACGTGGAATACGAGGTGGCGGCGCAACGCGCCAGCTGGATCACCCCGGTGCCGGGCGGCGTCGGG
CCGATGACCCGCGCCTGCCTGCTGGAAAATACCCTGCACGCCGCCGAACACCTGCACGACTGA
Translation:
MTAQLIDGKAIAANLRQQIAQRVTERRQQGLRVPGLAVILVGTDPASQVYVAHKRKDCEEVGFLSQAYDLPA
ETSQDDLLALIDRLNDDPAIDGILVQLPLPAHLDASLLLERIHPDKDVDGFHPYNIGRLAQRMPLLRPCTPK
GIMTLLASTGADLYGMDAVVVGASNIVGRPMALELLLGGCTVTVTHRFTRDLADHVSRADLVVVAAGKPGLV
KGEWIKEGAIVIDVGINRQADGRLVGDVEYEVAAQRASWITPVPGGVGPMTRACLLENTLHAAEHLHD*
AnnotationID:2747GeneID:2739
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 13:01:01
Gene Name:folD
Confidence Code:2
GeneProduct:5,10-methylene-tetrahydrofolate dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:methenyltetrahydrofolate cyclohydrolase
Functional Category Confidence Code:5
COGs:COG0190,pfam02882
Secondary Functional Category(ies):nucleotide biosynthesis and metabolism
EC Number:1.5.1.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67878.1|AE016419_7 (AE016782) 5,10-methylene-tetrahydrofolate
           dehydrogenase/cyclohydrolase [Pseudomonas putida KT2440]
          Length = 284

 Score =  498 bits (1282), Expect = e-140
 Identities = 246/283 (86%), Positives = 262/283 (92%)

>gb|AAO57202.1| (AE016869) methylenetetrahydrofolate
           dehydrogenase/methenyltetrahydrofolate cyclohydrolase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 284

 Score =  490 bits (1262), Expect = e-138
 Identities = 244/284 (85%), Positives = 262/284 (92%)

>gb|AAN54845.1|AE015623_7 (AE015623) methylenetetrahydrofolate
           dehydrogenase/methylenetetrahydrofolate cyclohydrolase
           [Shewanella oneidensis MR-1]
          Length = 284

 Score =  420 bits (1080), Expect = e-117
 Identities = 206/284 (72%), Positives = 239/284 (84%)








 
Structural Features:
COG0190, FolD, 5,10-methylene-tetrahydrofolate dehydrogenase/
Methenyl tetrahydrofolate cyclohydrolase [Coenzyme metabolism]
CD-Length = 283 residues,  99.3% aligned
Score =  412 bits (1060), Expect = 3e-116
Identities = 167/281 (59%), Positives = 205/281 (72%)

pfam02882, THF_DHG_CYH_C, Tetrahydrofolate dehydrogenase/cyclohydrolase, 
NAD(P)-binding domain.
CD-Length = 166 residues,  98.8% aligned
Score =  225 bits (576), Expect = 4e-60
Identities = 94/164 (57%), Positives = 114/164 (69%), Gaps = 5/164 (3%)








   
Genomic Context:






   
Comment:


















    ReferenceID:5481




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22625




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36519




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2739_cdd.html


    		




 
URL:

















    ReferenceID:36587




  
Author/Investigator(s):
        D'Ari L, Rabinowitz JC.





  
Title:
        Purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from Escherichia coli.




  
PubMed:
        1748668


MedLine:
        




    
Source:
J Biol Chem. 1991 Dec 15;266(35):23953-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=92084696&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:2739
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4472
                           PA1796_tr
                           translation of PA1796
                           284
                           99.99
                           99.99
                           0
                           1455.0
     

     
     
129397
                           gb|AAG05185.1|AE004605_7
                           (AE004605) 5,10-methylene-tetrahydrofolate dehydrogenase / cyclohydrolase [Pseudomonas aeruginosa PAO1]
                           284
                           99.99
                           99.99
                           0
                           1455.0
     

     
     
129398
                           gb|AAN67878.1|AE016419_7
                           (AE016782) 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase [Pseudomonas putida KT2440]
                           284
                           86.61
                           92.25
                           0
                           1281.0
     

     
     
129399
                           gb|AAO57202.1|
                           (AE016869) methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           284
                           85.91
                           92.25
                           0
                           1262.0
     

     
     
129400
                           gb|AAN54845.1|AE015623_7
                           (AE015623) methylenetetrahydrofolate dehydrogenase/methylenetetrahydrofolate cyclohydrolase [Shewanella oneidensis MR-1]
                           284
                           72.53
                           84.15
                           0
                           1080.0
     

     






      

          
GC ORFID: 45741How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1796Glimmer Score: 

          
Start: 1266274Stop: 1265420

          
Length: 855


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1796 translation of PA1796Homolog Bit Score 565.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene649 (ORF: BLASTX 493.0), psyr_15may02_Scaffold1_revised_gene262 (ORF: BLASTX 271.0), t_PA1796 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGCACAACTGATCGACGGCAAAGCGATCGCCGCCAACCTTCGCCAGCAGATAGCCCAACGCGTGACC 
GAGCGCCGCCAGCAAGGCCTGCGCGTTCCCGGCCTGGCGGTGATCCTGGTCGGCACCGATCCGGCCTCTCAG 
GTCTATGTGGCGCACAAGCGCAAGGACTGCGAGGAAGTCGGCTTTCTCTCCCAGGCCTACGATCTTCCCGCC 
GAAACCAGCCAGGACGACCTGCTGGCCCTGATCGACCGCCTGAACGACGATCCCGCCATCGACGGCATCCTG 
GTCCAGCTACCCCTGCCCGCCCACCTGGACGCCTCCCTGCTGCTGGAGCGTATCCACCCGGACAAGGACGTG 
GACGGTTTCCATCCCTACAACATCGGCCGCCTGGCCCAGCGCATGCCCCTGCTGCGCCCCTGCACCCCGAAA 
GGCATCATGACCCTGCTCGCCAGCACCGGCGCCGACCTGTACGGCATGGACGCGGTCGTGGTCGGCGCCTCG 
AACATCGTCGGCCGGCCCATGGCTCTGGAGTTGCTGCTGGGTGGCTGCACCGTCACCGTGACCCACCGCTTC 
ACCCGCGACCTGGCCGACCATGTGTCGCGCGCCGACCTGGTGGTGGTCGCTGCCGGCAAGCCGGGACTGGTC 
AAGGGCGAGTGGATCAAGGAAGGCGCCATCGTCATCGACGTCGGCATCAACCGCCAGGCCGACGGCCGCCTG 
GTCGGCGACGTGGAATACGAGGTGGCGGCGCAACGCGCCAGCTGGATCACCCCGGTGCCGGGCGGCGTCGGG 
CCGATGACCCGCGCCTGCCTGCTGGAAAATACCCTGCACGCCGCCGAACACCTGCACGACTGA