ViewGene
Locus Name:
GeneID:
Location:

GeneID:2738
TrivialName: PA14_44120
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_44120
PAO1 Ortholog Locus:PA1576
Sequence Length:867
Protein Length:288
Start: 3927697
Stop: 3926831
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04965.1|AE004586_3 (AE004586) probable 3-hydroxyisobutyrate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 285/288 (98%), Positives = 286/288 (99%)
Sequence:
ATGACTGCGGTCGCATTTCTCGGCCTGGGCACGATGGGCTACCCGATGGCCGGGCACCTGCAGCGCGAGGGC
TATCACGTCTGCGTCTACAATCGCTCGTCGGCCAAGGCGCTGCGCTGGGTCGAGGAGTACGCCGGACGATAC
GCCGATACGCCGCGCGAGGCCTGTGCCGGCGCCGAACTGGTGTTCTGCTGCGTCGGTAACGACGACGACCTG
CGTGCCGTGGCGCTGGGCGAGCAGGGCGCCTTCGCCGGAATGGCGCCGGGCAGCCTGTTCGTCGACCACACC
ACCGCTTCGGCGGAAGTCGCCCGCGAGCTGTCGCTGCTGGCTGCCGAGCGTGAGCTGGGCTTTCTCGACGCG
CCGGTCTCCGGCGGCCAGGCGGGCGCGGTCAACGGCGCCCTGACGGTGATGGTCGGTGGCGAGGAGGCCTTC
TATCGACGCGCCGAACCGCTGCTGCGCAGCTATGCGCGGATGGTCCGGCGAATGGGCGACGTGGGTAGCGGG
CAATTGACCAAGATGGTCAACCAGATCTGCGTCGCGGGGCTGCTCCAGGGCTTGGCCGAGGCGCTGCACTTC
GCCCAGTGCGCCGGTCTCGACGGCGAGGCGGCGATGCAGGTGATCGGCAAGGGCGCGGCCCAGTCCtGGCAA
CTGGAAAACCGCCACCAGAGCATGCTgGCCGGAGAGTTCGACTTCGGCTTCGCCGTGGACTGGATGCGCAAG
GACCTGTCGATCCTGCTCGCCGAGGCGCGACGCAACGGCGCGCAGTTGCCGGTCACGGCGCTGGTCGACCAG
TTCTACGCCGAAGTGCAGGCGATGGGCGGCGGTCGTTGGGATACCTCCAGCCTGATCGCCCGGCTGCGTTCG
TGA
Translation:
MTAVAFLGLGTMGYPMAGHLQREGYHVCVYNRSSAKALRWVEEYAGRYADTPREACAGAELVFCCVGNDDDL
RAVALGEQGAFAGMAPGSLFVDHTTASAEVARELSLLAAERELGFLDAPVSGGQAGAVNGALTVMVGGEEAF
YRRAEPLLRSYARMVRRMGDVGSGQLTKMVNQICVAGLLQGLAEALHFAQCAGLDGEAAMQVIGKGAAQSWQ
LENRHQSMLAGEFDFGFAVDWMRKDLSILLAEARRNGAQLPVTALVDQFYAEVQAMGGGRWDTSSLIARLRS
*
AnnotationID:2746GeneID:2738
AnnotatorUID: diggins
Modification Date/Time: 2005-05-18 07:07:50
Gene Name:
Confidence Code:3
GeneProduct:putative 3-hydroxyisobutyrate dehydrogenase
Cell Localization:()
Synonyms:PA1576
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2084
Secondary Functional Category(ies):putative enzyme
EC Number:1.1.1.31
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN55796.1|AE015716_6 (AE015716) 2-hydroxy-3-oxopropionate reductase [Shewanella
           oneidensis MR-1]
          Length = 291

 Score =  390 bits (1001), Expect = e-108
 Identities = 188/285 (65%), Positives = 229/285 (80%)

>emb|CAE34588.1| (BX640449) putative oxidoreductase [Bordetella bronchiseptica]
          Length = 301

 Score =  385 bits (988), Expect = e-106
 Identities = 187/284 (65%), Positives = 225/284 (79%)

>dbj|BAB48116.1| (AP002995) 3-hydroxyisobutyrate dehydrogenase [Mesorhizobium loti]
          Length = 289

 Score =  381 bits (978), Expect = e-105
 Identities = 189/287 (65%), Positives = 227/287 (79%), Gaps = 1/287 (0%)






 
Structural Features:
COG2084, MmsB, 3-hydroxyisobutyrate dehydrogenase 
and related beta-hydroxyacid dehydrogenases 
[Lipid metabolism]
CD-Length = 286 residues, 100.0% aligned
Score =  306 bits (785), Expect = 2e-84
Identities = 131/286 (45%), Positives = 175/286 (61%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5479




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22623




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37305




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2738_cdd.html


    		




 
URL:

















    ReferenceID:38141




  
Author/Investigator(s):
        Chowdhury EK, Akaishi Y, Nagata S, Misono H.





  
Title:
        Cloning and overexpression of the 3-hydroxyisobutyrate dehydrogenase gene from pseudomonas putida E23.




  
PubMed:
        12729017


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 2003 Feb;67(2):438-41.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12729017

















    ReferenceID:38142




  
Author/Investigator(s):
        Steele MI, Lorenz D, Hatter K, Park A, Sokatch JR.





  
Title:
        Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase.




  
PubMed:
        1339433


MedLine:
        




    
Source:
J Biol Chem. 1992 Jul 5;267(19):13585-92.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1339433









           
Homologs By Global Alignment 

           

       



     
Gene ID:2738
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4471
                           PA1576_tr
                           translation of PA1576
                           288
                           98.95
                           99.30
                           0
                           1457.0
     

     
     
129376
                           gb|AAG04965.1|AE004586_3
                           (AE004586) probable 3-hydroxyisobutyrate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           288
                           98.95
                           99.30
                           0
                           1457.0
     

     
     
129382
                           dbj|BAC49005.1|
                           (AP005948) dehydrogenase [Bradyrhizobium japonicum USDA 110]
                           290
                           65.51
                           78.62
                           2
                           980.0
     

     
     
129380
                           dbj|BAB48116.1|
                           (AP002995) 3-hydroxyisobutyrate dehydrogenase [Mesorhizobium loti]
                           289
                           65.39
                           78.54
                           1
                           980.0
     

     
     
129379
                           emb|CAE27414.1|
                           (BX572599) 3-hydroxyisobutyrate dehydrogenase [Rhodopseudomonas palustris CGA009]
                           289
                           65.39
                           77.85
                           1
                           981.0
     

     






      

          
GC ORFID: 45740How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1576Glimmer Score: 

          
Start: 1026212Stop: 1027078

          
Length: 867


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1576 translation of PA1576Homolog Bit Score 565.0

          
Other Homologs:  t_PA1576 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTGCGGTCGCATTTCTCGGCCTGGGCACGATGGGCTACCCGATGGCCGGGCACCTGCAGCGCGAGGGC 
TATCACGTCTGCGTCTACAATCGCTCGTCGGCCAAGGCGCTGCGCTGGGTCGAGGAGTACGCCGGACGATAC 
GCCGATACGCCGCGCGAGGCCTGTGCCGGCGCCGAACTGGTGTTCTGCTGCGTCGGTAACGACGACGACCTG 
CGTGCCGTGGCGCTGGGCGAGCAGGGCGCCTTCGCCGGAATGGCGCCGGGCAGCCTGTTCGTCGACCACACC 
ACCGCTTCGGCGGAAGTCGCCCGCGAGCTGTCGCTGCTGGCTGCCGAGCGTGAGCTGGGCTTTCTCGACGCG 
CCGGTCTCCGGCGGCCAGGCGGGCGCGGTCAACGGCGCCCTGACGGTGATGGTCGGTGGCGAGGAGGCCTTC 
TATCGACGCGCCGAACCGCTGCTGCGCAGCTATGCGCGGATGGTCCGGCGAATGGGCGACGTGGGTAGCGGG 
CAATTGACCAAGATGGTCAACCAGATCTGCGTCGCGGGGCTGCTCCAGGGCTTGGCCGAGGCGCTGCACTTC 
GCCCAGTGCGCCGGTCTCGACGGCGAGGCGGCGATGCAGGTGATCGGCAAGGGCGCGGCCCAGTCCtGGCAA 
CTGGAAAACCGCCACCAGAGCATGCTgGCCGGAGAGTTCGACTTCGGCTTCGCCGTGGACTGGATGCGCAAG 
GACCTGTCGATCCTGCTCGCCGAGGCGCGACGCAACGGCGCGCAGTTGCCGGTCACGGCGCTGGTCGACCAG 
TTCTACGCCGAAGTGCAGGCGATGGGCGGCGGTCGTTGGGATACCTCCAGCCTGATCGCCCGGCTGCGTTCG 
TGA