ViewGene
Locus Name:
GeneID:
Location:

GeneID:2725
TrivialName: PA14_27480
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_27480
PAO1 Ortholog Locus:PA2830
Sequence Length:876
Protein Length:291
Start: 2386397
Stop: 2385522
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06218.1|AE004709_11 (AE004709) heat shock protein HtpX [Pseudomonas aeruginosa PAO1]
Identities = 291/291 (100%), Positives = 291/291 (100%)
Sequence:
ATGATGCGCATCCTGTTGTTCCTGGCCACCAACCTGGCAGTCCTGGTGATTGCCAGCATCACCCTGAAACTG
CTCGGGGTGGACCGCTTCACCGGCCAGAATTACGGCAGCCTGCTGGTCTTCTGCGCCGTGTTCGGTTTCGCC
GGTTCGCTGGTCTCGCTGTTCATCTCCAAGTGGATGGCGAAGATGAGCACCGGTACCGAAGTCATCAGCCAG
CCGCGCACCCGTCACGAACAGTGGCTGCTGCAGACCGTCGAAGAGCTGTCCCGCGAAGCCGGCATCAAGATG
CCGGAGGTCGGCATCTTCCCCGCCTACGAGGCCAACGCCTTCGCCACCGGCTGGAACAAGAACGACGCGCTG
GTCGCGGTCAGCCAGGGCCTGCTCGAACGTTTCTCGCCCGATGAGGTGAAAGCCGTCCTCGCCCACGAGATC
GGCCACGTCGCCAACGGCGACATGGTCACCCTGGCGTTGATCCAGGGCGTGGTGAACACCTTCGTGATGTTC
TTCGCGCGAATCTTCGGCAACTTCGTCGACAAGGCGATCCTGAAGAACGAGGACGGCCCGGGCATCGGCTAC
TTCGTCGCGACCATCTTCGCCGAACTGGTGCTCGGCATCCTCGCCAGCATCATCGTCATGTGGTTCTCGCGC
CGCCGCGAGTTCCGCGCCGACGCCGCCGGCGCCCACCTGGCTGGCACCGGCGCGATGATCGCCGCGCTGCAA
CGCCTGCGTTCGGAACAGGGCGTGCCGGTGCAGATGCCCGACACCCTGAACGCCTTCGGCATCAATGGCGGC
CTCAAGCACGGCCTGGCCGGCCTGCTGATGAGCCACCCGCCGCTGGAAGATCGTATCGAAGCGCTGCGCGCC
AGCGCACGCTGA
Translation:
MMRILLFLATNLAVLVIASITLKLLGVDRFTGQNYGSLLVFCAVFGFAGSLVSLFISKWMAKMSTGTEVISQ
PRTRHEQWLLQTVEELSREAGIKMPEVGIFPAYEANAFATGWNKNDALVAVSQGLLERFSPDEVKAVLAHEI
GHVANGDMVTLALIQGVVNTFVMFFARIFGNFVDKAILKNEDGPGIGYFVATIFAELVLGILASIIVMWFSR
RREFRADAAGAHLAGTGAMIAALQRLRSEQGVPVQMPDTLNAFGINGGLKHGLAGLLMSHPPLEDRIEALRA
SAR*
AnnotationID:2733GeneID:2725
AnnotatorUID: diggins
Modification Date/Time: 2005-04-06 15:03:02
Gene Name:htpX
Confidence Code:2
GeneProduct:heat shock protein HtpX
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:Zn-dependent protease with chaperone function
Functional Category Confidence Code:5
COGs:COG0501,pfam06509
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:3.4.24.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67490.1|AE016375_8 (AE016780) heat shock protein HtpX [Pseudomonas putida KT2440]
          Length = 295

 Score =  488 bits (1257), Expect = e-137
 Identities = 248/292 (84%), Positives = 265/292 (90%), Gaps = 5/292 (1%)

>gb|AAO55298.1| (AE016862) heat shock protein HtpX [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 295

 Score =  474 bits (1219), Expect = e-133
 Identities = 243/292 (83%), Positives = 257/292 (88%), Gaps = 5/292 (1%)

>gb|AAM41571.1| (AE012336) heat shock protein [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 292

 Score =  303 bits (775), Expect = 1e-81
 Identities = 157/288 (54%), Positives = 202/288 (70%), Gaps = 5/288 (1%)








 
Structural Features:
COG0501, HtpX, Zn-dependent protease with chaperone function 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 302 residues,  92.4% aligned
Score =  170 bits (431), Expect = 2e-43
Identities = 93/294 (31%), Positives = 132/294 (44%), Gaps = 20/294 (6%)

pfam06509, HtpX_N, HtpX N-terminus.
CD-Length = 76 residues, 100.0% aligned
Score =  112 bits (281), Expect = 7e-26
Identities = 45/79 (56%), Positives = 55/79 (69%), Gaps = 3/79 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5453




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22597




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36241




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2725_cdd.html


    		




 
URL:

















    ReferenceID:36399




  
Author/Investigator(s):
        Kornitzer D, Teff D, Altuvia S, Oppenheim AB.





  
Title:
        Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX.




  
PubMed:
        1826904


MedLine:
        




    
Source:
J Bacteriol. 1991 May;173(9):2944-53.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1826904









           
Homologs By Global Alignment 

           

       



     
Gene ID:2725
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4454
                           PA2830_tr
                           translation of PA2830
                           291
                           99.99
                           99.99
                           0
                           1463.0
     

     
     
129140
                           gb|AAG06218.1|AE004709_11
                           (AE004709) heat shock protein HtpX [Pseudomonas aeruginosa PAO1]
                           291
                           99.99
                           99.99
                           0
                           1463.0
     

     
     
129141
                           gb|AAN67490.1|AE016375_8
                           (AE016780) heat shock protein HtpX [Pseudomonas putida KT2440]
                           296
                           83.78
                           89.52
                           6
                           1259.0
     

     
     
129142
                           gb|AAO55298.1|
                           (AE016862) heat shock protein HtpX [Pseudomonas syringae pv. tomato str. DC3000]
                           296
                           82.09
                           86.82
                           6
                           1221.0
     

     
     
129143
                           gb|AAP12369.1|
                           (AY259120) HtpX [Pseudomonas sp. Hsa.28]
                           291
                           64.60
                           67.35
                           84
                           947.0
     

     






      

          
GC ORFID: 45727How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2830Glimmer Score: 

          
Start: 2567530Stop: 2568405

          
Length: 876


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2830 translation of PA2830Homolog Bit Score 568.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7667 (ORF: BLASTX 478.0), t_PA2830 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATGCGCATCCTGTTGTTCCTGGCCACCAACCTGGCAGTCCTGGTGATTGCCAGCATCACCCTGAAACTG 
CTCGGGGTGGACCGCTTCACCGGCCAGAATTACGGCAGCCTGCTGGTCTTCTGCGCCGTGTTCGGTTTCGCC 
GGTTCGCTGGTCTCGCTGTTCATCTCCAAGTGGATGGCGAAGATGAGCACCGGTACCGAAGTCATCAGCCAG 
CCGCGCACCCGTCACGAACAGTGGCTGCTGCAGACCGTCGAAGAGCTGTCCCGCGAAGCCGGCATCAAGATG 
CCGGAGGTCGGCATCTTCCCCGCCTACGAGGCCAACGCCTTCGCCACCGGCTGGAACAAGAACGACGCGCTG 
GTCGCGGTCAGCCAGGGCCTGCTCGAACGTTTCTCGCCCGATGAGGTGAAAGCCGTCCTCGCCCACGAGATC 
GGCCACGTCGCCAACGGCGACATGGTCACCCTGGCGTTGATCCAGGGCGTGGTGAACACCTTCGTGATGTTC 
TTCGCGCGAATCTTCGGCAACTTCGTCGACAAGGCGATCCTGAAGAACGAGGACGGCCCGGGCATCGGCTAC 
TTCGTCGCGACCATCTTCGCCGAACTGGTGCTCGGCATCCTCGCCAGCATCATCGTCATGTGGTTCTCGCGC 
CGCCGCGAGTTCCGCGCCGACGCCGCCGGCGCCCACCTGGCTGGCACCGGCGCGATGATCGCCGCGCTGCAA 
CGCCTGCGTTCGGAACAGGGCGTGCCGGTGCAGATGCCCGACACCCTGAACGCCTTCGGCATCAATGGCGGC 
CTCAAGCACGGCCTGGCCGGCCTGCTGATGAGCCACCCGCCGCTGGAAGATCGTATCGAAGCGCTGCGCGCC 
AGCGCACGCTGA