ViewGene
Locus Name:
GeneID:
Location:

GeneID:2719
TrivialName: PA14_44570
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_44570
PAO1 Ortholog Locus:PA1537
Sequence Length:888
Protein Length:295
Start: 3967658
Stop: 3968545
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04926.1|AE004582_4 (AE004582) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 291/295 (98%), Positives = 293/295 (99%)
Sequence:
ATGAAGTCGTTCGAAAACAAAGTCGCCGCCATCACCGGCGCAGGCTCGGGCATCGGTCGCGCCCTGGCCGTG
GAGCTGGGCAGGCAGGGTTGCCATCTGGCCCTGGCCGACGTCAATGCCGCAGCTCTCGAGGAAACCCGCCAA
TTGCTCGCCAGCAGCGGGGTACGCGTCTCCACCGCGGTCGTCGACGTGGCCGACCGCGAGCAGGTGCAGGCC
TGGGCCGACAAGGCAGCCAGCGAACATGGCCGGGTGAACCTGATCTTCAACAACGCGGGCGTCGCCCACGCC
GGTACCGTGGAAGGCAGCGACTACTCGGAATACGAGTGGATCATGAACATCAACTTCTGGGGCGTGGTGAAC
GGCACCAAGGCGTTCCTTCCGCACCTGAAGGCCTCCGGCAACGGCCACGTGGTCAATGTCTCCAGCGTCTTC
GGGCTGTTCGCCCAGCCCGGCATGAGCGCCTACAACGCCACCAAGTACGCAGTGCGTGGCTTCACCGAGTCG
TTGCGCCAGGAACTGGACATGGAGGACTCCGGCGTCTCCGCCAGTTGCGTGCATCCCGGCGGGATCAAGACC
AACATCGCCAAGACCGCGCGAATGAACGAGAGCATGGCCAAGGTCACCGGCCAAGCTGCCGACAAGGCTCGC
CAGCAATTCAACGACCAGTTGCTGCGGACCACCCCGGAGAAGGCCGCCCAGGTCATCCTGCGCGGCGTGCAG
CGCGACAGCCGGCGCATCCTGATCGGCGCCGACGCCCACGCGATCGACGTGATGCTGCGCCTGGCGCCAGTG
CTCTACCAGCGCGTGGTCACCGCCAGCATGCGCCTGGCAGCGCGCTTCGCGCCGCGCCCGAAGAGCCCCCAG
GGCGCCCGCGAAGCCAGCGAGTGA
Translation:
MKSFENKVAAITGAGSGIGRALAVELGRQGCHLALADVNAAALEETRQLLASSGVRVSTAVVDVADREQVQA
WADKAASEHGRVNLIFNNAGVAHAGTVEGSDYSEYEWIMNINFWGVVNGTKAFLPHLKASGNGHVVNVSSVF
GLFAQPGMSAYNATKYAVRGFTESLRQELDMEDSGVSASCVHPGGIKTNIAKTARMNESMAKVTGQAADKAR
QQFNDQLLRTTPEKAAQVILRGVQRDSRRILIGADAHAIDVMLRLAPVLYQRVVTASMRLAARFAPRPKSPQ
GAREASE*
AnnotationID:2727GeneID:2719
AnnotatorUID: diggins
Modification Date/Time: 2005-02-25 08:08:43
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:PA1537
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00106,COG0300,COG4221
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL79772.1| (AF090329) alcohol dehydrogenase [Pseudomonas fluorescens]
          Length = 296

 Score =  336 bits (862), Expect = 8e-92
 Identities = 168/290 (57%), Positives = 225/290 (77%), Gaps = 7/290 (2%)

>gb|AAK45541.1| (AE007004) oxidoreductase, short-chain dehydrogenase/reductase
           family [Mycobacterium tuberculosis CDC1551]
          Length = 276

 Score =  261 bits (667), Expect = 3e-69
 Identities = 131/285 (45%), Positives = 188/285 (65%), Gaps = 9/285 (3%)






 
Structural Features:
pfam00106, adh_short, short chain dehydrogenase. 
CD-Length = 247 residues,  91.1% aligned
Score =  166 bits (422), Expect = 3e-42
Identities = 81/228 (35%), Positives = 122/228 (53%), Gaps = 3/228 (1%)

COG0300, DltE, Short-chain dehydrogenases of various substrate specificities 
CD-Length = 265 residues,  93.6% aligned
Score =  159 bits (404), Expect = 3e-40
Identities = 79/264 (29%), Positives = 123/264 (46%), Gaps = 17/264 (6%)

COG4221, COG4221, Short-chain alcohol dehydrogenase of unknown specificity
CD-Length = 246 residues,  83.3% aligned
Score =  154 bits (391), Expect = 1e-38
Identities = 69/209 (33%), Positives = 110/209 (52%), Gaps = 4/209 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5441




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22585




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37300




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2719_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2719
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4446
                           PA1537_tr
                           translation of PA1537
                           295
                           98.64
                           99.32
                           0
                           1467.0
     

     
     
129086
                           gb|AAG04926.1|AE004582_4
                           (AE004582) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           295
                           98.64
                           99.32
                           0
                           1467.0
     

     
     
129089
                           gb|AAL79772.1|
                           (AF090329) alcohol dehydrogenase [Pseudomonas fluorescens]
                           303
                           56.76
                           75.24
                           15
                           866.0
     

     
     
129087
                           gb|AAK00851.1|AF298896_1
                           (AF298896) hypothetical protein [Burkholderia pseudomallei]
                           312
                           54.80
                           71.79
                           21
                           867.0
     

     
     
129088
                           gb|AAR13804.1|
                           (AY452488) alcoholdehydrogenase [Pseudomonas frederiksbergensis]
                           344
                           50.00
                           66.27
                           56
                           866.0
     

     






      

          
GC ORFID: 45721How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1537Glimmer Score: 

          
Start: 986251Stop: 985364

          
Length: 888


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1537 translation of PA1537Homolog Bit Score 569.0

          
Other Homologs:  t_PA3277 (ORF: BLASTX 127.0), t_PA1537 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGTCGTTCGAAAACAAAGTCGCCGCCATCACCGGCGCAGGCTCGGGCATCGGTCGCGCCCTGGCCGTG 
GAGCTGGGCAGGCAGGGTTGCCATCTGGCCCTGGCCGACGTCAATGCCGCAGCTCTCGAGGAAACCCGCCAA 
TTGCTCGCCAGCAGCGGGGTACGCGTCTCCACCGCGGTCGTCGACGTGGCCGACCGCGAGCAGGTGCAGGCC 
TGGGCCGACAAGGCAGCCAGCGAACATGGCCGGGTGAACCTGATCTTCAACAACGCGGGCGTCGCCCACGCC 
GGTACCGTGGAAGGCAGCGACTACTCGGAATACGAGTGGATCATGAACATCAACTTCTGGGGCGTGGTGAAC 
GGCACCAAGGCGTTCCTTCCGCACCTGAAGGCCTCCGGCAACGGCCACGTGGTCAATGTCTCCAGCGTCTTC 
GGGCTGTTCGCCCAGCCCGGCATGAGCGCCTACAACGCCACCAAGTACGCAGTGCGTGGCTTCACCGAGTCG 
TTGCGCCAGGAACTGGACATGGAGGACTCCGGCGTCTCCGCCAGTTGCGTGCATCCCGGCGGGATCAAGACC 
AACATCGCCAAGACCGCGCGAATGAACGAGAGCATGGCCAAGGTCACCGGCCAAGCTGCCGACAAGGCTCGC 
CAGCAATTCAACGACCAGTTGCTGCGGACCACCCCGGAGAAGGCCGCCCAGGTCATCCTGCGCGGCGTGCAG 
CGCGACAGCCGGCGCATCCTGATCGGCGCCGACGCCCACGCGATCGACGTGATGCTGCGCCTGGCGCCAGTG 
CTCTACCAGCGCGTGGTCACCGCCAGCATGCGCCTGGCAGCGCGCTTCGCGCCGCGCCCGAAGAGCCCCCAG 
GGCGCCCGCGAAGCCAGCGAGTGA