ViewGene
Locus Name:
GeneID:
Location:

GeneID:2712
TrivialName: PA14_36870
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_36870
PAO1 Ortholog Locus:PA2142
Sequence Length:861
Protein Length:286
Start: 3286682
Stop: 3285822
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05530.1|AE004641_9 (AE004641) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 284/286 (99%), Positives = 286/286 (100%)
Sequence:
ATGAGCGAACAGCGACAGACCCTGCCGGCGCAGCACCAGGATCAACGTCCCGGCCACGAAAGCGAGATGCAG
CCGAAGCCGGAGTTCGTTTCCGCCGATTACCGTCCGGCGGCCAAGCTGGAGGGTAAGGTGGCCCTGGTCACC
GGCGGCGACAGTGGTATCGGCCGCGCGGTGGCGGTGGCTTTCGCCCGCGAGAAGGCGGATGTGCTGCTGGTC
TACCTGGATGAGAACGAAGACGCGGCGAAGACCCGCGAGATCATCGAGTCGCTCGGCCGCCAGTGCCTGGCG
TTCGCCGGCGACGTCGCCGACGCCGGCTTCTGCCGCCAGGTGGTGGACACGCTAAGGCAGAAATGGGGGCGC
CTCGACGTGCTGGTCAACAACGCCGGCGAGCAGCATCCGCAGGCGCGCCTGGAGGACATCAGCGAAGAGCAG
TGGGAGAAGACCTTCCGCACCAACATCTTCGGCATGTTCCAGTTGACCAAGGCGGCCCTGCCGCTGATGGGC
AAGGGCGGGGCGATCATCAACACCACCTCGATCACCGCCTACAAGGGCAACCCCCAGCTGATCGACTATTCC
TCGACCAAGGGCGCGATCACCTCGTTCACCCGGTCGCTGTCGATGAACCTGGTCAACCGCGGCATCCGCGTC
AACGCCGTGGCCCCGGGCCCGATCTGGACCCCGCTGATCCCGTCCACCTTCAGCGCCGAGAAGGTCGCCCAC
TTCGGCGCGGATACGCCGATGGGCCGGCCCGGCCAGCCGGAAGAGCTGGCCGCCAGCTACGTCTACCTGGCC
TGTAACGACTCGTCCTACGTCAGCGGCCAGGTGCTGCACGTCAACGGCGGTACGGTGGTCAACGGCTGA
Translation:
MSEQRQTLPAQHQDQRPGHESEMQPKPEFVSADYRPAAKLEGKVALVTGGDSGIGRAVAVAFAREKADVLLV
YLDENEDAAKTREIIESLGRQCLAFAGDVADAGFCRQVVDTLRQKWGRLDVLVNNAGEQHPQARLEDISEEQ
WEKTFRTNIFGMFQLTKAALPLMGKGGAIINTTSITAYKGNPQLIDYSSTKGAITSFTRSLSMNLVNRGIRV
NAVAPGPIWTPLIPSTFSAEKVAHFGADTPMGRPGQPEELAASYVYLACNDSSYVSGQVLHVNGGTVVNG*
AnnotationID:2720GeneID:2712
AnnotatorUID: diggins
Modification Date/Time: 2005-03-03 11:11:06
Gene Name:
Confidence Code:3
GeneProduct:putative short-chain dehydrogenase
Cell Localization:()
Synonyms:PA2142
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:putative oxidoreductase
Functional Category Confidence Code:5
COGs:COG1028,pfam00106
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAF12426.1|AE001863_51 (AE001863) oxidoreductase, short-chain dehydrogenase/reductase
           family [Deinococcus radiodurans]
          Length = 390

 Score =  365 bits (936), Expect = e-100
 Identities = 178/281 (63%), Positives = 223/281 (79%)

>dbj|BAC13142.1| (AP004597) oxidoreductase (short-chain dehydrogenase:reductase
           family) [Oceanobacillus iheyensis HTE831]
          Length = 290

 Score =  357 bits (915), Expect = 6e-98
 Identities = 169/280 (60%), Positives = 217/280 (77%)

>dbj|BAB82286.1| (AP003194) probable short-chain dehydrogenase [Clostridium
           perfringens str. 13]
          Length = 292

 Score =  322 bits (825), Expect = 2e-87
 Identities = 158/283 (55%), Positives = 206/283 (72%), Gaps = 3/283 (1%)






 
Structural Features:
COG1028, FabG, Dehydrogenases with different specificities 
(related to short-chain alcohol dehydrogenases) 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 251 residues,  98.8% aligned
Score =  213 bits (543), Expect = 2e-56
Identities = 110/249 (44%), Positives = 155/249 (62%), Gaps = 7/249 (2%)

pfam00106, adh_short, short chain dehydrogenase. 
CD-Length = 247 residues,  99.6% aligned
Score =  207 bits (529), Expect = 1e-54
Identities = 100/248 (40%), Positives = 138/248 (55%), Gaps = 13/248 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5427




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22572




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32582




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2712_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2712
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4439
                           PA2142_tr
                           translation of PA2142
                           286
                           99.30
                           99.99
                           0
                           1469.0
     

     
     
129002
                           gb|AAG05530.1|AE004641_9
                           (AE004641) probable short-chain dehydrogenase [Pseudomonas aeruginosa PAO1]
                           286
                           99.30
                           99.99
                           0
                           1469.0
     

     
     
129003
                           dbj|BAB76881.1|
                           (AP003599) oxidoreductase [Nostoc sp. PCC 7120]
                           286
                           63.98
                           79.72
                           1
                           962.0
     

     
     
129006
                           dbj|BAB05230.1|
                           (AP001512) oxidoreductase (short chain dehydrogenase/reductase family) [Bacillus halodurans]
                           287
                           62.71
                           77.35
                           1
                           926.0
     

     
     
129004
                           dbj|BAC88361.1|
                           (AP006569) glr0420 [Gloeobacter violaceus PCC 7421]
                           286
                           61.53
                           78.32
                           0
                           943.0
     

     






      

          
GC ORFID: 45714How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2142Glimmer Score: 

          
Start: 1667232Stop: 1668092

          
Length: 861


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2142 translation of PA2142Homolog Bit Score 570.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1345 (ORF: BLASTX 276.0), t_PA2142 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGAACAGCGACAGACCCTGCCGGCGCAGCACCAGGATCAACGTCCCGGCCACGAAAGCGAGATGCAG 
CCGAAGCCGGAGTTCGTTTCCGCCGATTACCGTCCGGCGGCCAAGCTGGAGGGTAAGGTGGCCCTGGTCACC 
GGCGGCGACAGTGGTATCGGCCGCGCGGTGGCGGTGGCTTTCGCCCGCGAGAAGGCGGATGTGCTGCTGGTC 
TACCTGGATGAGAACGAAGACGCGGCGAAGACCCGCGAGATCATCGAGTCGCTCGGCCGCCAGTGCCTGGCG 
TTCGCCGGCGACGTCGCCGACGCCGGCTTCTGCCGCCAGGTGGTGGACACGCTAAGGCAGAAATGGGGGCGC 
CTCGACGTGCTGGTCAACAACGCCGGCGAGCAGCATCCGCAGGCGCGCCTGGAGGACATCAGCGAAGAGCAG 
TGGGAGAAGACCTTCCGCACCAACATCTTCGGCATGTTCCAGTTGACCAAGGCGGCCCTGCCGCTGATGGGC 
AAGGGCGGGGCGATCATCAACACCACCTCGATCACCGCCTACAAGGGCAACCCCCAGCTGATCGACTATTCC 
TCGACCAAGGGCGCGATCACCTCGTTCACCCGGTCGCTGTCGATGAACCTGGTCAACCGCGGCATCCGCGTC 
AACGCCGTGGCCCCGGGCCCGATCTGGACCCCGCTGATCCCGTCCACCTTCAGCGCCGAGAAGGTCGCCCAC 
TTCGGCGCGGATACGCCGATGGGCCGGCCCGGCCAGCCGGAAGAGCTGGCCGCCAGCTACGTCTACCTGGCC 
TGTAACGACTCGTCCTACGTCAGCGGCCAGGTGCTGCACGTCAACGGCGGTACGGTGGTCAACGGCTGA