ViewGene
Locus Name:
GeneID:
Location:

GeneID:2682
TrivialName: PA14_10170
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10170
PAO1 Ortholog Locus:PA4159
Sequence Length:906
Protein Length:301
Start: 873423
Stop: 872518
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07546.1|AE004832_6 (AE004832) ferrienterobactin-binding periplasmic protein precursor FepB [Pseudomonas aeruginosa PAO1]
Identities = 300/301 (99%), Positives = 300/301 (99%)
Sequence:
ATGCCGACCCGCCGCCGCTCCGCCCTGCCATTACTCGCCCTCGCCCTGAGCCTGTTCGCCACCCTCGCCGCG
GCCGGGGAGCCGAAGCCGGCGCGGATCGTCTCCACCACCCCGAGCGTCACCGGCATCCTGCTGGCGATGGAC
GCACCGCTGGTGGCCAGCGCCGCCACCACGCCAAGTCGCCTGACCGACGCCAAGGGCTTCTTCTCGCAATGG
GCGAAGGTCGCCGACCAACGTGGCGTCGAGGTGCTCTACCGCAACCTGCGCTTCGACATCGAAGCGGTGATC
GCCCAGGATCCGGACCTGCTGGTCGCCTCCGCCACCGGCGCCGACAGCGCCGCGCCATACCGTGCGGAGCTG
GAGGCGCAGGGCGTACCGACGCTGGTGGTCGACTACTCGAAACACAGCTGGCAGGAACTGGCTACCGAGCTG
GGCCGGCACACCGGCCTGGAACGCCAGGCGCAGGCCGCCATCCAGCGCTTCGACGCCTACACCGCCGAAGTC
GCCGCGGCCATCGCGCCGCCGCAGGGCCCGGTCAGCGTGGTCGGCTACAACATCGCCGGCAGCTATTCCATC
GGCCGCCAGGCCAGCCCCCAGGCACGCCTGCTCGAAGCCCTCGGCTTCCAGGTGGCGGAACTGCCGGAGGCG
CTGGCGGGCAAGGTGACACGCGCCTCGGACTTCCAGTTCATCTCGCGGGAAAACCTGCCCGCCGCCATCGCC
GGCGACAGCGTGTTCCTGCTCGGCGCCAGCGACGACGACGTGCAGGCGTTCCTCGCCGACCCGGTGCTGGCC
AACCTGTCGGCGGTGCGCGAAAAGCGCGTGTACGCCCTGGGCCCCAGTTCCTTCCGCATCGACTACTACTCG
GGGCGGCAGATGATCGACAACGTCGCCCGCCATTTCCGCTGA
Translation:
MPTRRRSALPLLALALSLFATLAAAGEPKPARIVSTTPSVTGILLAMDAPLVASAATTPSRLTDAKGFFSQW
AKVADQRGVEVLYRNLRFDIEAVIAQDPDLLVASATGADSAAPYRAELEAQGVPTLVVDYSKHSWQELATEL
GRHTGLERQAQAAIQRFDAYTAEVAAAIAPPQGPVSVVGYNIAGSYSIGRQASPQARLLEALGFQVAELPEA
LAGKVTRASDFQFISRENLPAAIAGDSVFLLGASDDDVQAFLADPVLANLSAVREKRVYALGPSSFRIDYYS
GRQMIDNVARHFR*
AnnotationID:2690GeneID:2682
AnnotatorUID: diggins
Modification Date/Time: 2005-03-31 06:06:11
Gene Name:fepB
Confidence Code:2
GeneProduct:ferrienterobactin-binding periplasmic protein precursor FepB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4592,cd01146
Secondary Functional Category(ies):inorganic ion transport and metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO15900.1|AF508972_1 (AF508972) putative iron ABC transporter substrate-binding
           component [Corynebacterium glutamicum]
          Length = 333

 Score =  261 bits (668), Expect = 3e-69
 Identities = 136/269 (50%), Positives = 177/269 (65%), Gaps = 2/269 (0%)

>gb|AAK95844.1| (AY042783) periplasmic binding protein PhuB [Photorhabdus
           luminescens]
          Length = 346

 Score =  207 bits (527), Expect = 6e-53
 Identities = 117/273 (42%), Positives = 164/273 (60%), Gaps = 9/273 (3%)

>gb|AAO69876.1| (AE016841) ferrienterobactin-binding periplasmic protein precursor
           [Salmonella enterica subsp. enterica serovar Typhi Ty2]
          Length = 318

 Score =  206 bits (523), Expect = 2e-52
 Identities = 127/276 (46%), Positives = 168/276 (60%), Gaps = 6/276 (2%)






 
Structural Features:
COG4592, FepB, ABC-type Fe2+-enterobactin transport system, periplasmic component 
[Inorganic ion transport and metabolism]
CD-Length = 319 residues,  99.4% aligned
Score =  388 bits (997), Expect = 5e-109
Identities = 159/319 (49%), Positives = 200/319 (62%), Gaps = 20/319 (6%)

cd01146, FhuD, Fe3+-siderophore binding domain FhuD.
CD-Length = 256 residues,  99.6% aligned
Score =  144 bits (364), Expect = 1e-35







   
Genomic Context:






   
Comment:


















    ReferenceID:5367




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22512




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36900




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2682_cdd.html


    		




 
URL:

















    ReferenceID:36998




  
Author/Investigator(s):
        Elkins MF, Earhart CF.





  
Title:
        Nucleotide sequence and regulation of the Escherichia coli gene for ferrienterobactin transport protein FepB.




  
PubMed:
        2529253


MedLine:
        




    
Source:
J Bacteriol. 1989 Oct;171(10):5443-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2529253

















    ReferenceID:36999




  
Author/Investigator(s):
        Pierce JR, Earhart CF.





  
Title:
        Escherichia coli K-12 envelope proteins specifically required for ferrienterobactin uptake.




  
PubMed:
        3011753


MedLine:
        




    
Source:
J Bacteriol. 1986 Jun;166(3):930-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3011753









           
Homologs By Global Alignment 

           

       



     
Gene ID:2682
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4402
                           PA4159_tr
                           translation of PA4159
                           301
                           99.66
                           99.66
                           0
                           1483.0
     

     
     
128161
                           gb|AAG07546.1|AE004832_6
                           (AE004832) ferrienterobactin-binding periplasmic protein precursor FepB [Pseudomonas aeruginosa PAO1]
                           301
                           99.66
                           99.66
                           0
                           1483.0
     

     
     
128164
                           gb|AAO69876.1|
                           (AE016841) ferrienterobactin-binding periplasmic protein precursor [Salmonella enterica subsp. enterica serovar Typhi Ty2]
                           324
                           41.97
                           56.17
                           29
                           535.0
     

     
     
128165
                           emb|CAD05070.1|
                           (AL627267) ferrienterobactin-binding periplasmic protein precursor [Salmonella enterica subsp. enterica serovar Typhi]
                           324
                           41.97
                           56.17
                           29
                           535.0
     

     
     
128172
                           gb|AAL19545.1|
                           (AE008723) ferric enterobactin (enterochelin) tranporter [Salmonella typhimurium LT2]
                           321
                           41.43
                           55.76
                           23
                           532.0
     

     






      

          
GC ORFID: 45684How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4159Glimmer Score: 

          
Start: 4080514Stop: 4081419

          
Length: 906


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4159 translation of PA4159Homolog Bit Score 575.0

          
Other Homologs:  t_PA4159 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGACCCGCCGCCGCTCCGCCCTGCCATTACTCGCCCTCGCCCTGAGCCTGTTCGCCACCCTCGCCGCG 
GCCGGGGAGCCGAAGCCGGCGCGGATCGTCTCCACCACCCCGAGCGTCACCGGCATCCTGCTGGCGATGGAC 
GCACCGCTGGTGGCCAGCGCCGCCACCACGCCAAGTCGCCTGACCGACGCCAAGGGCTTCTTCTCGCAATGG 
GCGAAGGTCGCCGACCAACGTGGCGTCGAGGTGCTCTACCGCAACCTGCGCTTCGACATCGAAGCGGTGATC 
GCCCAGGATCCGGACCTGCTGGTCGCCTCCGCCACCGGCGCCGACAGCGCCGCGCCATACCGTGCGGAGCTG 
GAGGCGCAGGGCGTACCGACGCTGGTGGTCGACTACTCGAAACACAGCTGGCAGGAACTGGCTACCGAGCTG 
GGCCGGCACACCGGCCTGGAACGCCAGGCGCAGGCCGCCATCCAGCGCTTCGACGCCTACACCGCCGAAGTC 
GCCGCGGCCATCGCGCCGCCGCAGGGCCCGGTCAGCGTGGTCGGCTACAACATCGCCGGCAGCTATTCCATC 
GGCCGCCAGGCCAGCCCCCAGGCACGCCTGCTCGAAGCCCTCGGCTTCCAGGTGGCGGAACTGCCGGAGGCG 
CTGGCGGGCAAGGTGACACGCGCCTCGGACTTCCAGTTCATCTCGCGGGAAAACCTGCCCGCCGCCATCGCC 
GGCGACAGCGTGTTCCTGCTCGGCGCCAGCGACGACGACGTGCAGGCGTTCCTCGCCGACCCGGTGCTGGCC 
AACCTGTCGGCGGTGCGCGAAAAGCGCGTGTACGCCCTGGGCCCCAGTTCCTTCCGCATCGACTACTACTCG 
GGGCGGCAGATGATCGACAACGTCGCCCGCCATTTCCGCTGA