ViewGene
Locus Name:
GeneID:
Location:

GeneID:2672
TrivialName: PA14_03950
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03950
PAO1 Ortholog Locus:PA0303
Sequence Length:882
Protein Length:293
Start: 356417
Stop: 357298
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03692.1|AE004468_4 (AE004468) polyamine transport protein PotH [Pseudomonas aeruginosa PAO1]
Identities = 293/293 (100%), Positives = 293/293 (100%)
Sequence:
ATGCCCACCGGGCGGCATGCGGTCATCGGGGTGCCGTTCCTGTGGCTCTTCCTGTTCTTCCTGCTGCCCTTC
GCCATCGTGCTGAAGATCAGCCTGGCGGAAGCGGACGTGGCCATCCCGCCCTACACCGAGATCTTCGCCTAC
GCGGACCAGCAGTTGCAGGTGCTCCTCAACCTCGGCAACTACCTGATGCTGACCGATGACCCGCTCTACATC
GATGCCTACCTCGGCTCGCTGAAGATGGCCTTCATCAGTACCCTGATCTGCCTGCTGGTGGGCTACCCGATG
GCCTACGCCATCGCCCGCTCCAGCCAGTCCTCGCAGACTGTCCTGCTGCTGCTGATCATGATGCCGACCTGG
ACGGCGATCCTGATCCGGGTCTATGCCTGGATGGGCATCCTTTCCAACAACGGCCTGCTCAACAGCGTACTG
ATGGGGCTGGGGCTCATCAGCGAGCCGCTGACCATCCTCAATACCAACATCGCGGTGTACATCGGCATCGTC
TATTCCTACCTGCCGTTCATGATCCTGCCGCTCTACGCCAACCTGGTGAAGCACGACCCGAGCCTGCTGGAG
GCCGCTTCCGACCTGGGCGCGCGTAACTTCACCAGCTTCTGGAAGATCACCGTGCCGCTGTCGAAGAACGGC
ATCATCGCCGGCTGCATGCTGGTGTTCATCCCGGCGGTGGGCGAGTTCGTCATCCCGGAACTGCTCGGCGGC
CCCGAGACCCTGATGATCGGCAAGGTGCTCTGGCAGGAGTTCTTCAACAACCGCGACTGGCCGGTGGCGTCC
GCCCTGGCGGTGGTAATGCTGGCGATCCTGATCATCCCGATCATCCTGTTCAACAAGAACCAGGCCAAAGAA
CTGGAGGGCAAGGTATGA
Translation:
MPTGRHAVIGVPFLWLFLFFLLPFAIVLKISLAEADVAIPPYTEIFAYADQQLQVLLNLGNYLMLTDDPLYI
DAYLGSLKMAFISTLICLLVGYPMAYAIARSSQSSQTVLLLLIMMPTWTAILIRVYAWMGILSNNGLLNSVL
MGLGLISEPLTILNTNIAVYIGIVYSYLPFMILPLYANLVKHDPSLLEAASDLGARNFTSFWKITVPLSKNG
IIAGCMLVFIPAVGEFVIPELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILIIPIILFNKNQAKE
LEGKV*
AnnotationID:2680GeneID:2672
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 07:07:23
Gene Name:spuG
Confidence Code:1
GeneProduct:polyamine transport protein PotH
Cell Localization:()
Synonyms:potH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putrescine ABC transporter, permease protein
Functional Category Confidence Code:5
COGs:COG1176
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK15490.1|AF323694_5 (AF323694) PotH [Pseudomonas fluorescens]
          Length = 306

 Score =  497 bits (1279), Expect = e-140
 Identities = 243/291 (83%), Positives = 266/291 (91%)

>gb|AAO58728.1| (AE016875) putrescine ABC transporter, permease protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 317

 Score =  492 bits (1266), Expect = e-138
 Identities = 241/291 (82%), Positives = 263/291 (90%)

>gb|AAN54340.1|AE015571_3 (AE015571) polyamine ABC transporter, permease protein [Shewanella
           oneidensis MR-1]
          Length = 301

 Score =  422 bits (1086), Expect = e-118
 Identities = 207/287 (72%), Positives = 240/287 (83%)






 
Structural Features:
COG1176, PotB, ABC-type spermidine/putrescine transport system, 
permease component I 
[Amino acid transport and metabolism]
CD-Length = 287 residues,  97.6% aligned
Score =  262 bits (670), Expect = 5e-71
Identities = 146/291 (50%), Positives = 192/291 (65%), Gaps = 11/291 (3%)








   
Genomic Context:






   
Comment:


















    ReferenceID:5347




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22492




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33654




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2672_cdd.html


    		




 
URL:

















    ReferenceID:35428




  
Author/Investigator(s):
        Lu CD, Itoh Y, Nakada Y, Jiang Y.





  
Title:
        Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1.




  
PubMed:
        12081945


MedLine:
        




    
Source:
J Bacteriol. 2002 Jul;184(14):3765-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12081945









           
Homologs By Global Alignment 

           

       



     
Gene ID:2672
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4390
                           PA0303_tr
                           translation of PA0303
                           293
                           99.99
                           99.99
                           0
                           1487.0
     

     
     
127987
                           gb|AAG03692.1|AE004468_4
                           (AE004468) polyamine transport protein PotH [Pseudomonas aeruginosa PAO1]
                           293
                           99.99
                           99.99
                           0
                           1487.0
     

     
     
127988
                           gb|AAK15490.1|AF323694_5
                           (AF323694) PotH [Pseudomonas fluorescens]
                           306
                           79.41
                           86.92
                           13
                           1276.0
     

     
     
127990
                           gb|AAN70743.1|AE016718_2
                           (AE016793) putrescine ABC transporter, permease protein [Pseudomonas putida KT2440]
                           306
                           77.45
                           87.25
                           13
                           1266.0
     

     
     
127989
                           gb|AAO58728.1|
                           (AE016875) putrescine ABC transporter, permease protein [Pseudomonas syringae pv. tomato str. DC3000]
                           317
                           76.02
                           82.96
                           24
                           1265.0
     

     






      

          
GC ORFID: 45674How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0303Glimmer Score: 

          
Start: 6157759Stop: 6158640

          
Length: 882


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0303 translation of PA0303Homolog Bit Score 577.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6514 (ORF: BLASTX 495.0), t_PA3608 (ORF: BLASTX 215.0), t_PA0303 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCACCGGGCGGCATGCGGTCATCGGGGTGCCGTTCCTGTGGCTCTTCCTGTTCTTCCTGCTGCCCTTC 
GCCATCGTGCTGAAGATCAGCCTGGCGGAAGCGGACGTGGCCATCCCGCCCTACACCGAGATCTTCGCCTAC 
GCGGACCAGCAGTTGCAGGTGCTCCTCAACCTCGGCAACTACCTGATGCTGACCGATGACCCGCTCTACATC 
GATGCCTACCTCGGCTCGCTGAAGATGGCCTTCATCAGTACCCTGATCTGCCTGCTGGTGGGCTACCCGATG 
GCCTACGCCATCGCCCGCTCCAGCCAGTCCTCGCAGACTGTCCTGCTGCTGCTGATCATGATGCCGACCTGG 
ACGGCGATCCTGATCCGGGTCTATGCCTGGATGGGCATCCTTTCCAACAACGGCCTGCTCAACAGCGTACTG 
ATGGGGCTGGGGCTCATCAGCGAGCCGCTGACCATCCTCAATACCAACATCGCGGTGTACATCGGCATCGTC 
TATTCCTACCTGCCGTTCATGATCCTGCCGCTCTACGCCAACCTGGTGAAGCACGACCCGAGCCTGCTGGAG 
GCCGCTTCCGACCTGGGCGCGCGTAACTTCACCAGCTTCTGGAAGATCACCGTGCCGCTGTCGAAGAACGGC 
ATCATCGCCGGCTGCATGCTGGTGTTCATCCCGGCGGTGGGCGAGTTCGTCATCCCGGAACTGCTCGGCGGC 
CCCGAGACCCTGATGATCGGCAAGGTGCTCTGGCAGGAGTTCTTCAACAACCGCGACTGGCCGGTGGCGTCC 
GCCCTGGCGGTGGTAATGCTGGCGATCCTGATCATCCCGATCATCCTGTTCAACAAGAACCAGGCCAAAGAA 
CTGGAGGGCAAGGTATGA