ViewGene
Locus Name:
GeneID:
Location:

GeneID:267
TrivialName: PA14_21110
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_21110
PAO1 Ortholog Locus:PA3319
Sequence Length:2079
Protein Length:692
Start: 1824476
Stop: 1826554
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06707.1|AE004754_3 (AE004754) non-hemolytic phospholipase C precursor [Pseudomonas aeruginosa PAO1]
Identities = 690/692 (99%), Positives = 692/692 (100%)
Sequence:
ATGATTTCGAAAAGCAGAAGAAGCTTCATCCGCCTGGCCGCCGGTACGGTCGGCGCCACCGTCGCCACCAGC
ATGCTGCCGTCGAGCATCCAGGCGGCCCTGGCGATTCCCGCGCACCGCCGGCATGGCAACCTCAAGGACGTC
GAGCACGTGGTGATCCTGATGCAGGAGAACCGCTCCTTCGACCACTATTTCGGCACCCTCAAGGGCGTCCGC
GGCTTCGGCGACCGCATGGCGATCCCGCTGCCCGACGGCCAGCGGGTCTGGCACCAGAAGGGCAGCAAGGGC
GAGATCCTGCCCTACCACTTCGACACCAGCACCACCAGCGCCCAGCGCGTCGACGGTACCCCGCACACCTGG
CCGGACGCCCAGCAGGCCTGGAACGAAGGGCGCATGGACAAGTGGTTGCCGGCCAAGACCGAGCGTTCCCTG
GGCTACTACAAGGAGCAGGACATCGCCTTCCAGTTCGCGATGGCCAACGCCTTCACCATCTGCGACGCCTAT
CACTGCTCGTTCCAGGGCGGCACCAACCCCAACCGGCTGTTCCTCTGGACCGGCACCAACGACCCGCTCGGC
CAGCATGGCGGCCCGGTAACCACCAACGACCACGACAGCAACGGCCCGGTGGAGCAGGGCTACACCTGGACC
ACCTATCCCGAGCGCCTGCAGGCCGCCGGCATCACCTGGCGGGTCTACCAGGACATGGCCGACAACTTCTCC
GACAACCCGCTGATCGGCTTCCGCCAGTACCGCGCCGCGGCGCCTGACTCGCCGCTGATCGTCAACGGCCTG
AGCACCTGGAAGCTCGATGCGCTGAAGCGCGACGTGCTGGCCAACAGCCTGCCGCAGGTGTCCTGGATCGTC
GCCCCGGCCAAGTACTCCGAACACCCCGGCCCGTCCAGCCCGATCTGGGGGGCCGAGTACACTTCCTGGGTA
CTCGACGCGCTGACCGCCAACCCGGAGGTCTGGAGCAAGACCGCGCTGCTGGTGATGTTCGACGAGAACGAC
GGCTTCTTCGACCACATCGCCCCGCCGGCCGCGCCGAGCCTGAACAAGGACGGCACGCTGCGCGGCAAGACC
ACCGCCGACGCCACCCTCGAATGGCACACCAAGGGCGATATCCGTTATCGCAACCAGCCCTACGGCCTCGGC
CCGCGGGTGCCGATGTACGTGATCTCGCCGTGGAGCAAGGGCGGCTGGGTCAACTCCCAGGTGTTCGACCAC
ACCTCGGTGATCCGCTTCCTGGAGCAGCGCTTCGGGGTCATGGAGCCGAATATCAGTCCCTGGCGTCGTGCG
GTCTGCGGCGACCTGACCTCGGCGTTCAACTTCGCCAACCCGAACAACGAGCCGTTCCCCGAACTGCCCGAC
ACCAGCCAGGCCGACGCCATCGTCGCCAGCCAGATCAAGCTGCCGAAGCCGAAGCCGCCGGCGGTGGCCGCC
ATGCCCAAGCAGGAAATGGGCATCCGTCCGGCCCGCGCCTTGCCCTACGAGTTGGGCGTGCATGCGCGCTAC
CGCAGCGGCGGAGACGCGCTGAGCCTGACCTTCGCCAACACCGGCAAGGCCGGCGCGGTGTTCCAGGTGTTC
GACCTGCTCGACAGCGAGAACCCGCCGAAACGCTACACCGTTGGCGCGCGCAAGCGCCTGCACGACAGCTTC
CAGGGCGACGCCAGCGGCGACTACCACCTGGAAGTGCACGGTCCGAACGGTTTCCTCCGGGTCTTCCGCGGC
AACCTGCGGCGCGACCTGGCGGAGCGCAAGGCGCCGCTGCCGGAAGTGCGGATCGACTACGAGCCGCTGTTC
GGCAACCTGCGCGTGCAACTGATCAACCGTGGCCGCCATCCGGTCAAGCTGACGGTCAAGGACAACGTCTAT
CGCCAGGGCGAGCGGCGTACCGTCAACGTGCCGCCGGGACAGCGTCGGGAAGTCCGCTATTCGCTGCGCAGC
AGCGGCAACTGGTACGACTTCAGCGTCAGCGCGCAGGGGTCGGACAGCTTCCTGCGGCGTTTCAGCGGTCGC
ATGGAAGATGGTCGCTCCGGCTTCAGCGACCCGGGCATGGGCCTGGGCACGCTGACCTTCTGA
Translation:
MISKSRRSFIRLAAGTVGATVATSMLPSSIQAALAIPAHRRHGNLKDVEHVVILMQENRSFDHYFGTLKGVR
GFGDRMAIPLPDGQRVWHQKGSKGEILPYHFDTSTTSAQRVDGTPHTWPDAQQAWNEGRMDKWLPAKTERSL
GYYKEQDIAFQFAMANAFTICDAYHCSFQGGTNPNRLFLWTGTNDPLGQHGGPVTTNDHDSNGPVEQGYTWT
TYPERLQAAGITWRVYQDMADNFSDNPLIGFRQYRAAAPDSPLIVNGLSTWKLDALKRDVLANSLPQVSWIV
APAKYSEHPGPSSPIWGAEYTSWVLDALTANPEVWSKTALLVMFDENDGFFDHIAPPAAPSLNKDGTLRGKT
TADATLEWHTKGDIRYRNQPYGLGPRVPMYVISPWSKGGWVNSQVFDHTSVIRFLEQRFGVMEPNISPWRRA
VCGDLTSAFNFANPNNEPFPELPDTSQADAIVASQIKLPKPKPPAVAAMPKQEMGIRPARALPYELGVHARY
RSGGDALSLTFANTGKAGAVFQVFDLLDSENPPKRYTVGARKRLHDSFQGDASGDYHLEVHGPNGFLRVFRG
NLRRDLAERKAPLPEVRIDYEPLFGNLRVQLINRGRHPVKLTVKDNVYRQGERRTVNVPPGQRREVRYSLRS
SGNWYDFSVSAQGSDSFLRRFSGRMEDGRSGFSDPGMGLGTLTF*
AnnotationID:277GeneID:267
AnnotatorUID: diggins
Modification Date/Time: 2005-04-15 08:08:34
Gene Name:plcN
Confidence Code:1
GeneProduct:non-hemolytic phospholipase C precursor
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam04185,COG3511
Secondary Functional Category(ies):cell wall / LPS /capsule
EC Number:3.1.4.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK24993.1| (AE005965) phospholipase C [Caulobacter crescentus CB15]
          Length = 686

 Score =  675 bits (1742), Expect = 0.0
 Identities = 364/699 (52%), Positives = 451/699 (64%), Gaps = 61/699 (8%)

>gb|AAF17299.1| (AF107252) non-hemolytic phospholipase C [Burkholderia
           pseudomallei]
          Length = 700

 Score =  612 bits (1577), Expect = e-174
 Identities = 342/718 (47%), Positives = 434/718 (60%), Gaps = 58/718 (8%)








 
Structural Features:
pfam04185, Phosphoesterase, Phosphoesterase family.
CD-Length = 376 residues, 100.0% aligned
Score =  381 bits (979), Expect = 2e-106
Identities = 159/409 (38%), Positives = 206/409 (50%), Gaps = 49/409 (11%)

COG3511, PlcC, Phospholipase C [Cell envelope biogenesis, outer membrane]
CD-Length = 527 residues, 100.0% aligned
Score =  638 bits (1647), Expect = 0.0
Identities = 262/546 (47%), Positives = 309/546 (56%), Gaps = 31/546 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:537




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17679




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32244




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:267_cdd.html


    		




 
URL:

















    ReferenceID:34022




  
Author/Investigator(s):
        Ostroff RM, Vasil AI, Vasil ML.





  
Title:
        Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa.




  
PubMed:
        2120196


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5915-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2120196









           
Homologs By Global Alignment 

           

       



     
Gene ID:267
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
445
                           PA3319_tr
                           translation of PA3319
                           692
                           99.71
                           99.99
                           0
                           3719.0
     

     
     
23822
                           gb|AAG06707.1|AE004754_3
                           (AE004754) non-hemolytic phospholipase C precursor [Pseudomonas aeruginosa PAO1]
                           692
                           99.71
                           99.99
                           0
                           3719.0
     

     
     
23823
                           gb|AAA25969.1|
                           (M30223) non-hemolytic phospholipase C precursor [Pseudomonas aeruginosa]
                           692
                           99.13
                           99.56
                           0
                           3695.0
     

     
     
23824
                           gb|AAA25968.1|
                           (M59304) phospholipase C [Pseudomonas aeruginosa]
                           692
                           99.13
                           99.56
                           0
                           3695.0
     

     
     
23825
                           gb|AAK24993.1|
                           (AE005965) phospholipase C [Caulobacter crescentus CB15]
                           729
                           50.89
                           63.23
                           80
                           1791.0
     

     






      

          
GC ORFID: 43269How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3319Glimmer Score: 

          
Start: 3129456Stop: 3127378

          
Length: 2079


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3319 translation of PA3319Homolog Bit Score 1437.0

          
Other Homologs:  t_PA0844 (ORF: BLASTX 501.0), t_PA3319 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATTTCGAAAAGCAGAAGAAGCTTCATCCGCCTGGCCGCCGGTACGGTCGGCGCCACCGTCGCCACCAGC 
ATGCTGCCGTCGAGCATCCAGGCGGCCCTGGCGATTCCCGCGCACCGCCGGCATGGCAACCTCAAGGACGTC 
GAGCACGTGGTGATCCTGATGCAGGAGAACCGCTCCTTCGACCACTATTTCGGCACCCTCAAGGGCGTCCGC 
GGCTTCGGCGACCGCATGGCGATCCCGCTGCCCGACGGCCAGCGGGTCTGGCACCAGAAGGGCAGCAAGGGC 
GAGATCCTGCCCTACCACTTCGACACCAGCACCACCAGCGCCCAGCGCGTCGACGGTACCCCGCACACCTGG 
CCGGACGCCCAGCAGGCCTGGAACGAAGGGCGCATGGACAAGTGGTTGCCGGCCAAGACCGAGCGTTCCCTG 
GGCTACTACAAGGAGCAGGACATCGCCTTCCAGTTCGCGATGGCCAACGCCTTCACCATCTGCGACGCCTAT 
CACTGCTCGTTCCAGGGCGGCACCAACCCCAACCGGCTGTTCCTCTGGACCGGCACCAACGACCCGCTCGGC 
CAGCATGGCGGCCCGGTAACCACCAACGACCACGACAGCAACGGCCCGGTGGAGCAGGGCTACACCTGGACC 
ACCTATCCCGAGCGCCTGCAGGCCGCCGGCATCACCTGGCGGGTCTACCAGGACATGGCCGACAACTTCTCC 
GACAACCCGCTGATCGGCTTCCGCCAGTACCGCGCCGCGGCGCCTGACTCGCCGCTGATCGTCAACGGCCTG 
AGCACCTGGAAGCTCGATGCGCTGAAGCGCGACGTGCTGGCCAACAGCCTGCCGCAGGTGTCCTGGATCGTC 
GCCCCGGCCAAGTACTCCGAACACCCCGGCCCGTCCAGCCCGATCTGGGGGGCCGAGTACACTTCCTGGGTA 
CTCGACGCGCTGACCGCCAACCCGGAGGTCTGGAGCAAGACCGCGCTGCTGGTGATGTTCGACGAGAACGAC 
GGCTTCTTCGACCACATCGCCCCGCCGGCCGCGCCGAGCCTGAACAAGGACGGCACGCTGCGCGGCAAGACC 
ACCGCCGACGCCACCCTCGAATGGCACACCAAGGGCGATATCCGTTATCGCAACCAGCCCTACGGCCTCGGC 
CCGCGGGTGCCGATGTACGTGATCTCGCCGTGGAGCAAGGGCGGCTGGGTCAACTCCCAGGTGTTCGACCAC 
ACCTCGGTGATCCGCTTCCTGGAGCAGCGCTTCGGGGTCATGGAGCCGAATATCAGTCCCTGGCGTCGTGCG 
GTCTGCGGCGACCTGACCTCGGCGTTCAACTTCGCCAACCCGAACAACGAGCCGTTCCCCGAACTGCCCGAC 
ACCAGCCAGGCCGACGCCATCGTCGCCAGCCAGATCAAGCTGCCGAAGCCGAAGCCGCCGGCGGTGGCCGCC 
ATGCCCAAGCAGGAAATGGGCATCCGTCCGGCCCGCGCCTTGCCCTACGAGTTGGGCGTGCATGCGCGCTAC 
CGCAGCGGCGGAGACGCGCTGAGCCTGACCTTCGCCAACACCGGCAAGGCCGGCGCGGTGTTCCAGGTGTTC 
GACCTGCTCGACAGCGAGAACCCGCCGAAACGCTACACCGTTGGCGCGCGCAAGCGCCTGCACGACAGCTTC 
CAGGGCGACGCCAGCGGCGACTACCACCTGGAAGTGCACGGTCCGAACGGTTTCCTCCGGGTCTTCCGCGGC 
AACCTGCGGCGCGACCTGGCGGAGCGCAAGGCGCCGCTGCCGGAAGTGCGGATCGACTACGAGCCGCTGTTC 
GGCAACCTGCGCGTGCAACTGATCAACCGTGGCCGCCATCCGGTCAAGCTGACGGTCAAGGACAACGTCTAT 
CGCCAGGGCGAGCGGCGTACCGTCAACGTGCCGCCGGGACAGCGTCGGGAAGTCCGCTATTCGCTGCGCAGC 
AGCGGCAACTGGTACGACTTCAGCGTCAGCGCGCAGGGGTCGGACAGCTTCCTGCGGCGTTTCAGCGGTCGC 
ATGGAAGATGGTCGCTCCGGCTTCAGCGACCCGGGCATGGGCCTGGGCACGCTGACCTTCTGA