ViewGene
Locus Name:
GeneID:
Location:

GeneID:2665
TrivialName: PA14_23840
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23840
PAO1 Ortholog Locus:PA3114
Sequence Length:858
Protein Length:285
Start: 2072879
Stop: 2073736
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06502.1|AE004735_9 (AE004735) tRNA-pseudouridine synthase I [Pseudomonas aeruginosa PAO1]
Identities = 284/285 (99%), Positives = 284/285 (99%)
Sequence:
ATGAACGATGTTGTTCCGCCAGCGGCAGCCGAATCGGCTGCCGCTGGCGTTTCCAGAATCGCCCTCGGCATC
GAGTACAAGGGCTCCCGCTATCGTGGCTGGCAGCGCCAGGAAGCCGGCGTGCCTTCCGTGCAGGAAGCGCTG
GAGCGCGCCTTGTCGAAGGTTGCCGCCGAGCCGGTCGGGGTGATCTGCGCCGGCCGTACCGACGCTGCCGTT
CATGCCAGCGGCCAGGTAGTGCATTTCGACACCGCGGTGGAGCGCCCGTTGAAAGCATGGGTGATGGGCACC
AACGCCAACCTGCCCGCGGATATCAGTGTGACCTGGGCGAGAGTGATGCCGGCGCATTTCCACGCGCGCTTC
TCGGCCATGGCCCGGCGCTATCGCTACGCCATCTACAACGATCCGATCCGCCCGGCGCACCTGGCCGAGGAG
GTCACCTGGAACCATCGGCCGCTGGATATCGGGCGGATGCGCGAGGCGGCCCAGGTACTGGTCGGCACCCAT
GACTTCACCTCGTTCCGGGCGGTGCAGTGCCAGGCCAAGTCGCCGGTCAAGACCATGCATCACGTGCGCTTG
CTGGAGCATGGTCGGTTGATCGTGCTGGACATCCGCGGTAACGCTTTCCTTCACCATATGGTGCGCAACATC
GCTGGCGTCCTGATGACCATCGGTGCCGGCGAGCGGCCGATCGAATGGGCGAAGGAGGTGCTCGAGACGCGC
GACCGGCGCGCCGGTGGGGTCACCGCTCACCCCTACGGACTCTATCTGGTGCGGGTGGAGTACCCCGGGGAG
TTCGAGTTGCCCGAGCGCTACCTGGGGCCGCATTTCCTTTCCGGATTGCCCGATATCGTCGGCTGA
Translation:
MNDVVPPAAAESAAAGVSRIALGIEYKGSRYRGWQRQEAGVPSVQEALERALSKVAAEPVGVICAGRTDAAV
HASGQVVHFDTAVERPLKAWVMGTNANLPADISVTWARVMPAHFHARFSAMARRYRYAIYNDPIRPAHLAEE
VTWNHRPLDIGRMREAAQVLVGTHDFTSFRAVQCQAKSPVKTMHHVRLLEHGRLIVLDIRGNAFLHHMVRNI
AGVLMTIGAGERPIEWAKEVLETRDRRAGGVTAHPYGLYLVRVEYPGEFELPERYLGPHFLSGLPDIVG*
AnnotationID:2673GeneID:2665
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 07:07:31
Gene Name:truA
Confidence Code:2
GeneProduct:tRNA-pseudouridine synthase I
Cell Localization:()
Synonyms:hisT
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:pseudouridylate synthase I
Functional Category Confidence Code:5
COGs:COG0101
Secondary Functional Category(ies):
EC Number:4.2.1.70
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO10390.1|AE016803_177 (AE016803) tRNA pseudouridine synthase A [Vibrio vulnificus CMCP6]
          Length = 264

 Score =  309 bits (792), Expect = 1e-83
 Identities = 155/259 (59%), Positives = 185/259 (71%), Gaps = 1/259 (0%)

>gb|AAL21269.1| (AE008806) pseudouridylate synthase I [Salmonella typhimurium LT2]
          Length = 270

 Score =  305 bits (781), Expect = 2e-82
 Identities = 150/265 (56%), Positives = 188/265 (70%), Gaps = 1/265 (0%)

>gb|AAN81315.1|AE016763_274 (AE016763) tRNA pseudouridine synthase A [Escherichia coli CFT073]
          Length = 270

 Score =  303 bits (775), Expect = 1e-81
 Identities = 148/262 (56%), Positives = 187/262 (71%), Gaps = 1/262 (0%)






 
Structural Features:
COG0101, TruA, Pseudouridylate synthase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 266 residues,  99.6% aligned
Score =  334 bits (859), Expect = 5e-93
Identities = 152/266 (57%), Positives = 185/266 (69%), Gaps = 1/266 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5333




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22478




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32411




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2665_cdd.html


    		




 
URL:

















    ReferenceID:33911




  
Author/Investigator(s):
        Ahn KS, Ha U, Jia J, Wu D, Jin S.





  
Title:
        The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes.




  
PubMed:
        14993303


MedLine:
        




    
Source:
Microbiology. 2004 Mar;150(Pt 3):539-47.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14993303









           
Homologs By Global Alignment 

           

       



     
Gene ID:2665
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4383
                           PA3114_tr
                           translation of PA3114
                           285
                           99.64
                           99.64
                           0
                           1488.0
     

     
     
127908
                           gb|AAG06502.1|AE004735_9
                           (AE004735) tRNA-pseudouridine synthase I [Pseudomonas aeruginosa PAO1]
                           285
                           99.64
                           99.64
                           0
                           1488.0
     

     
     
127909
                           gb|AAC23941.1|
                           (U93274) HisT [Pseudomonas aeruginosa]
                           285
                           99.64
                           99.64
                           0
                           1488.0
     

     
     
127910
                           gb|AAO10390.1|AE016803_177
                           (AE016803) tRNA pseudouridine synthase A [Vibrio vulnificus CMCP6]
                           285
                           55.08
                           65.96
                           21
                           793.0
     

     
     
127911
                           dbj|BAC95189.1|
                           (AP005339) tRNA pseudouridine synthase A [Vibrio vulnificus YJ016]
                           285
                           55.08
                           65.96
                           21
                           793.0
     

     






      

          
GC ORFID: 45667How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3114Glimmer Score: 

          
Start: 2881052Stop: 2880195

          
Length: 858


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3114 translation of PA3114Homolog Bit Score 577.0

          
Other Homologs:  psyr_15may02_Scaffold16_revised_gene2624 (ORF: BLASTX 439.0), t_PA3114 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACGATGTTGTTCCGCCAGCGGCAGCCGAATCGGCTGCCGCTGGCGTTTCCAGAATCGCCCTCGGCATC 
GAGTACAAGGGCTCCCGCTATCGTGGCTGGCAGCGCCAGGAAGCCGGCGTGCCTTCCGTGCAGGAAGCGCTG 
GAGCGCGCCTTGTCGAAGGTTGCCGCCGAGCCGGTCGGGGTGATCTGCGCCGGCCGTACCGACGCTGCCGTT 
CATGCCAGCGGCCAGGTAGTGCATTTCGACACCGCGGTGGAGCGCCCGTTGAAAGCATGGGTGATGGGCACC 
AACGCCAACCTGCCCGCGGATATCAGTGTGACCTGGGCGAGAGTGATGCCGGCGCATTTCCACGCGCGCTTC 
TCGGCCATGGCCCGGCGCTATCGCTACGCCATCTACAACGATCCGATCCGCCCGGCGCACCTGGCCGAGGAG 
GTCACCTGGAACCATCGGCCGCTGGATATCGGGCGGATGCGCGAGGCGGCCCAGGTACTGGTCGGCACCCAT 
GACTTCACCTCGTTCCGGGCGGTGCAGTGCCAGGCCAAGTCGCCGGTCAAGACCATGCATCACGTGCGCTTG 
CTGGAGCATGGTCGGTTGATCGTGCTGGACATCCGCGGTAACGCTTTCCTTCACCATATGGTGCGCAACATC 
GCTGGCGTCCTGATGACCATCGGTGCCGGCGAGCGGCCGATCGAATGGGCGAAGGAGGTGCTCGAGACGCGC 
GACCGGCGCGCCGGTGGGGTCACCGCTCACCCCTACGGACTCTATCTGGTGCGGGTGGAGTACCCCGGGGAG 
TTCGAGTTGCCCGAGCGCTACCTGGGGCCGCATTTCCTTTCCGGATTGCCCGATATCGTCGGCTGA