ViewGene
Locus Name:
GeneID:
Location:

GeneID:2660
TrivialName: PA14_51270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_51270
PAO1 Ortholog Locus:PA1010
Sequence Length:879
Protein Length:292
Start: 4556680
Stop: 4555802
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04399.1|AE004533_10 (AE004533) dihydrodipicolinate synthase [Pseudomonas aeruginosa PAO1]
Identities = 292/292 (100%), Positives = 292/292 (100%)
Sequence:
ATGATTGCGGGCAGTATGGTGGCACTGGTCACCCCCTTCGATGCTCAGGGTCGACTGGACTGGGACAGCCTC
GCCAAGCTGGTGGACTTCCACTTGCAGGAAGGCACCAACGCCATCGTCGCCGTCGGCACCACCGGTGAATCG
GCCACGCTGGACGTGGAAGAGCACATCCAGGTGATCCGTCGCGTGGTCGACCAGGTCAAGGGCCGCATCCCG
GTGATCGCCGGCACCGGCGCCAACTCCACCCGCGAGGCGGTGGCGCTGACCGAGGCCGCGAAAAGCGGCGGC
GCCGACGCCTGCCTGCTGGTGACGCCGTACTACAACAAGCCGACCCAGGAAGGCATGTACCAGCACTTCCGC
CATATCGCCGAAGCCGTTGCGATCCCGCAGATCCTCTACAACGTGCCCGGGCGTACCTCCTGTGACATGCTT
CCGGAGACCGTCGAGCGCCTGTCCAAGGTGCCGAACATCATCGGCATCAAGGAGGCCACCGGCGACCTGCAA
CGCGCCAAGGAAGTCATCGAGCGCGTCGGCAAGGACTTCCTCGTCTATTCCGGCGACGACGCCACTGCCGTC
GAGCTGATGCTGCTGGGTGGCAAGGGCAACATCTCGGTGACCGCCAACGTCGCGCCGCGCGCCATGAGCGAC
CTGTGCGCCGCCGCCATGCGCGGCGACGCCGCTGCCGCGCGCGCCATCAACGACCGCCTGATGCCGCTGCAC
AAGGCGTTGTTCATCGAATCCAACCCCATTCCGGTGAAGTGGGCCCTGCATGAGATGGGTCTGATCCCCGAG
GGCATCCGCCTGCCCCTGACCTGGCTCAGCCCGCGTTGCCACGAGCCGCTGCGTCAGGCCATGCGCCAGACC
GGCGTATTGGCTTGA
Translation:
MIAGSMVALVTPFDAQGRLDWDSLAKLVDFHLQEGTNAIVAVGTTGESATLDVEEHIQVIRRVVDQVKGRIP
VIAGTGANSTREAVALTEAAKSGGADACLLVTPYYNKPTQEGMYQHFRHIAEAVAIPQILYNVPGRTSCDML
PETVERLSKVPNIIGIKEATGDLQRAKEVIERVGKDFLVYSGDDATAVELMLLGGKGNISVTANVAPRAMSD
LCAAAMRGDAAAARAINDRLMPLHKALFIESNPIPVKWALHEMGLIPEGIRLPLTWLSPRCHEPLRQAMRQT
GVLA*
AnnotationID:2668GeneID:2660
AnnotatorUID: diggins
Modification Date/Time: 2006-01-22 09:09:08
Gene Name:dapA
Confidence Code:2
GeneProduct:dihydrodipicolinate synthase
Cell Localization:(1) Cytoplasmic
Synonyms:PA1010
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00701, COG0329, cd00950, cd00408
Secondary Functional Category(ies):
EC Number:4.2.1.52
Status:ACTIVE
Pathway:
Amino-acid biosynthesis; L-lysine biosynthesis via DAP
pathway; 
tetrahydrodipicolinate from L-aspartate: step 3.
Homology:
>gb|AAO57414.1| (AE016870) dihydrodipicolinate synthase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 292

 Score =  497 bits (1280), Expect = e-140
 Identities = 249/291 (85%), Positives = 266/291 (91%)

>gb|AAN66861.1|AE016314_8 (AE016778) dihydrodipicolinate synthase [Pseudomonas putida KT2440]
          Length = 295

 Score =  448 bits (1152), Expect = e-125
 Identities = 228/294 (77%), Positives = 254/294 (86%), Gaps = 3/294 (1%)

>dbj|BAA16365.1| (D90877) dihydrodipicolinate synthase (EC 4.2.1.52) [Escherichia
           coli]
          Length = 292

 Score =  333 bits (854), Expect = 7e-91
 Identities = 167/292 (57%), Positives = 213/292 (72%), Gaps = 1/292 (0%)
Structural Features:
cd00950, DHDPS, Dihydrodipicolinate synthase (DHDPS) is a key 
enzyme in lysine biosynthesis.
CD-Length = 284 residues, 100.0% aligned
Score =  417 bits (1074), Expect = 7e-118

cd00408, DHDPS-like, Dihydrodipicolinate synthase family.
CD-Length = 281 residues,  99.6% aligned
Score =  331 bits (851), Expect = 5e-92

pfam00701, DHDPS, Dihydrodipicolinate synthetase family. 
CD-Length = 289 residues, 100.0% aligned
Score =  353 bits (907), Expect = 2e-98
Identities = 153/289 (52%), Positives = 198/289 (68%)

COG0329, DapA, Dihydrodipicolinate synthase/N-acetylneuraminate lyase 
[Amino acid transport and metabolism / 
Cell envelope biogenesis, outer membrane].
CD-Length = 299 residues,  98.0% aligned
Score =  344 bits (884), Expect = 6e-96
Identities = 149/293 (50%), Positives = 199/293 (67%), Gaps = 2/293 (0%)
Genomic Context:






   
Comment:


















    ReferenceID:5323




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22468




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38256




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2660_cdd.html


    		




 
URL:

















    ReferenceID:38257




  
Author/Investigator(s):
        Richaud F, Richaud C, Ratet P, Patte JC.





  
Title:
        Chromosomal location and nucleotide sequence of the Escherichia coli dapA gene.




  
PubMed:
        3514578


MedLine:
        




    
Source:
J Bacteriol. 1986 Apr;166(1):297-300.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=3514578

















    ReferenceID:38258




  
Author/Investigator(s):
        Bonnassie S, Oreglia J, Sicard AM.





  
Title:
        Nucleotide sequence of the dapA gene from Corynebacterium glutamicum.




  
PubMed:
        2129555


MedLine:
        




    
Source:
Nucleic Acids Res. 1990 Nov 11;18(21):6421.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=2129555









           
Homologs By Global Alignment 

           

       



     
Gene ID:2660
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4378
                           PA1010_tr
                           translation of PA1010
                           292
                           99.99
                           99.99
                           0
                           1488.0
     

     
     
127786
                           gb|AAG04399.1|AE004533_10
                           (AE004533) dihydrodipicolinate synthase [Pseudomonas aeruginosa PAO1]
                           292
                           99.99
                           99.99
                           0
                           1488.0
     

     
     
127787
                           gb|AAO57414.1|
                           (AE016870) dihydrodipicolinate synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           292
                           85.27
                           91.09
                           0
                           1280.0
     

     
     
127788
                           gb|AAN66861.1|AE016314_8
                           (AE016778) dihydrodipicolinate synthase [Pseudomonas putida KT2440]
                           295
                           77.28
                           86.10
                           3
                           1155.0
     

     
     
127790
                           emb|CAD14847.1|
                           (AL646062) PROBABLE DIHYDRODIPICOLINATE SYNTHASE PROTEIN [Ralstonia solanacearum]
                           294
                           60.54
                           75.51
                           2
                           899.0
     

     






      

          
GC ORFID: 45662How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1010Glimmer Score: 

          
Start: 397227Stop: 398105

          
Length: 879


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1010 translation of PA1010Homolog Bit Score 577.0

          
Other Homologs:  psyr_15may02_Scaffold10_revised_gene1725 (ORF: BLASTX 497.0), t_PA1010 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATTGCGGGCAGTATGGTGGCACTGGTCACCCCCTTCGATGCTCAGGGTCGACTGGACTGGGACAGCCTC 
GCCAAGCTGGTGGACTTCCACTTGCAGGAAGGCACCAACGCCATCGTCGCCGTCGGCACCACCGGTGAATCG 
GCCACGCTGGACGTGGAAGAGCACATCCAGGTGATCCGTCGCGTGGTCGACCAGGTCAAGGGCCGCATCCCG 
GTGATCGCCGGCACCGGCGCCAACTCCACCCGCGAGGCGGTGGCGCTGACCGAGGCCGCGAAAAGCGGCGGC 
GCCGACGCCTGCCTGCTGGTGACGCCGTACTACAACAAGCCGACCCAGGAAGGCATGTACCAGCACTTCCGC 
CATATCGCCGAAGCCGTTGCGATCCCGCAGATCCTCTACAACGTGCCCGGGCGTACCTCCTGTGACATGCTT 
CCGGAGACCGTCGAGCGCCTGTCCAAGGTGCCGAACATCATCGGCATCAAGGAGGCCACCGGCGACCTGCAA 
CGCGCCAAGGAAGTCATCGAGCGCGTCGGCAAGGACTTCCTCGTCTATTCCGGCGACGACGCCACTGCCGTC 
GAGCTGATGCTGCTGGGTGGCAAGGGCAACATCTCGGTGACCGCCAACGTCGCGCCGCGCGCCATGAGCGAC 
CTGTGCGCCGCCGCCATGCGCGGCGACGCCGCTGCCGCGCGCGCCATCAACGACCGCCTGATGCCGCTGCAC 
AAGGCGTTGTTCATCGAATCCAACCCCATTCCGGTGAAGTGGGCCCTGCATGAGATGGGTCTGATCCCCGAG 
GGCATCCGCCTGCCCCTGACCTGGCTCAGCCCGCGTTGCCACGAGCCGCTGCGTCAGGCCATGCGCCAGACC 
GGCGTATTGGCTTGA