ViewGene
Locus Name:
GeneID:
Location:

GeneID:2657
TrivialName: PA14_29020
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29020
PAO1 Ortholog Locus:PA2717
Sequence Length:831
Protein Length:276
Start: 2509058
Stop: 2509888
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06105.1|AE004700_1 (AE004700) chloroperoxidase precursor [Pseudomonas aeruginosa PAO1]
Identities = 275/276 (99%), Positives = 275/276 (99%)
Sequence:
ATGGGTTACGTGACAACGAAGGATGGCGTCGAACTCTTCTACAAGGACTGGGGGCCGCGCGACGCCCAGGTG
ATCTACTTCCATCATGGCTGGCCACTGAGTTCCGACGACTGGGATGCGCAGATGCTGTTCTTCCTCGCCGAG
GGCTTTCGCGTAGTGGCCCACGACCGCCGTGGCCACGGTCGTTCCAGCCAGGTCTGGGACGGCCATGACATG
GACCACTACGCCGACGACGTGGCGGCGGTGGTCGAGCGCCTCGGGGTGCGCGGGGCGATCCATGTCGGCCAT
TCCACCGGCGGCGGCGAGGTCGTccaCTACATCGCCCGCTATCCCGACGACCCGGTGCCGAAGGCGGCGATC
ATCAGCGCGGTACCGCCGCTGATGGTGAAGACCGAGGGCAATCCGGGCGGCCTGCCGAAGAGCGTCTTCGAC
GATCTCCAGGCGCAGCTCGCGGCCAATCGCGCGCAGTTCTACCAGGACATTCCCGCCGGCCCGTTCTATGGC
TACAACCGTCCCGGGGCGCAGCCCTCGGAAGGGATCGTCCGCAACTGGTGGCGGCAGGGCATGATGGGCGGC
GCCAAGGCCCACTACGATGGGATCGTGGCCTTTTCCCAGACCGACTTCAGCGATGACCTGAAGCGCATCGAC
ATCCCGGTGCTGGTGATGCATGGCGACGACGACCAGATCGTGCCCTACGAGAATTCCGGCGTACTCTCGGCG
AAGCTGTTGCGCAACGGCACGCTGAAGATCTATCCGGGGTTCCCGCACGGCATGCCGACCACCCAGGCCGAG
GTGATCAACGCCGACCTGCTGGCCTTCATCCGCGGCTGA
Translation:
MGYVTTKDGVELFYKDWGPRDAQVIYFHHGWPLSSDDWDAQMLFFLAEGFRVVAHDRRGHGRSSQVWDGHDM
DHYADDVAAVVERLGVRGAIHVGHSTGGGEVVHYIARYPDDPVPKAAIISAVPPLMVKTEGNPGGLPKSVFD
DLQAQLAANRAQFYQDIPAGPFYGYNRPGAQPSEGIVRNWWRQGMMGGAKAHYDGIVAFSQTDFSDDLKRID
IPVLVMHGDDDQIVPYENSGVLSAKLLRNGTLKIYPGFPHGMPTTQAEVINADLLAFIRG*
AnnotationID:2665GeneID:2657
AnnotatorUID: diggins
Modification Date/Time: 2005-04-05 06:06:52
Gene Name:cpo
Confidence Code:2
GeneProduct:chloroperoxidase precursor
Cell Localization:()
Synonyms:est
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:putative esterase
Functional Category Confidence Code:5
COGs:COG0596
Secondary Functional Category(ies):putative enzyme
EC Number:1.11.1.10
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA86922.1| (AB034986) bromoperoxidase-esterase [Pseudomonas putida]
          Length = 276

 Score =  488 bits (1255), Expect = e-137
 Identities = 223/275 (81%), Positives = 249/275 (90%)

>dbj|BAC55927.1| (AB092339) dihydrocoumarin hydrolase [Acinetobacter calcoaceticus]
          Length = 276

 Score =  486 bits (1250), Expect = e-137
 Identities = 221/275 (80%), Positives = 250/275 (90%)

>dbj|BAC46516.1| (AP005939) non-heme haloperoxidase [Bradyrhizobium japonicum USDA
           110]
          Length = 274

 Score =  443 bits (1140), Expect = e-124
 Identities = 206/275 (74%), Positives = 236/275 (85%), Gaps = 2/275 (0%)






 
Structural Features:
COG0596, MhpC, Predicted hydrolases or acyltransferases 
(alpha/beta hydrolase superfamily)
CD-Length = 282 residues,  99.6% aligned
Score =  120 bits (299), Expect = 2e-28
Identities = 79/288 (27%), Positives = 114/288 (39%), Gaps = 19/288 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5317




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22462




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36316




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2657_cdd.html


    		




 
URL:

















    ReferenceID:36367




  
Author/Investigator(s):
        Wolfframm C, Lingens F, Mutzel R, van Pee KH.





  
Title:
        Chloroperoxidase-encoding gene from Pseudomonas pyrrocinia: sequence, expression in heterologous hosts, and purification of the enzyme.




  
PubMed:
        8344520


MedLine:
        




    
Source:
Gene. 1993 Aug 16;130(1):131-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8344520









           
Homologs By Global Alignment 

           

       



     
Gene ID:2657
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4374
                           PA2717_tr
                           translation of PA2717
                           276
                           99.63
                           99.63
                           0
                           1491.0
     

     
     
127734
                           gb|AAG06105.1|AE004700_1
                           (AE004700) chloroperoxidase precursor [Pseudomonas aeruginosa PAO1]
                           276
                           99.63
                           99.63
                           0
                           1491.0
     

     
     
127735
                           gb|AAN69615.1|AE016595_3
                           (AE016789) esterase [Pseudomonas putida KT2440]
                           276
                           81.88
                           90.57
                           0
                           1264.0
     

     
     
127736
                           gb|AAB35246.1|
                           (S79438) esterase [Pseudomonas putida]
                           276
                           81.52
                           90.21
                           0
                           1255.0
     

     
     
127737
                           dbj|BAA86922.1|
                           (AB034986) bromoperoxidase-esterase [Pseudomonas putida]
                           276
                           80.79
                           90.21
                           0
                           1255.0
     

     






      

          
GC ORFID: 45659How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2717Glimmer Score: 

          
Start: 2444868Stop: 2444038

          
Length: 831


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2717 translation of PA2717Homolog Bit Score 578.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5862 (ORF: BLASTX 262.0), t_PA2717 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGTTACGTGACAACGAAGGATGGCGTCGAACTCTTCTACAAGGACTGGGGGCCGCGCGACGCCCAGGTG 
ATCTACTTCCATCATGGCTGGCCACTGAGTTCCGACGACTGGGATGCGCAGATGCTGTTCTTCCTCGCCGAG 
GGCTTTCGCGTAGTGGCCCACGACCGCCGTGGCCACGGTCGTTCCAGCCAGGTCTGGGACGGCCATGACATG 
GACCACTACGCCGACGACGTGGCGGCGGTGGTCGAGCGCCTCGGGGTGCGCGGGGCGATCCATGTCGGCCAT 
TCCACCGGCGGCGGCGAGGTCGTccaCTACATCGCCCGCTATCCCGACGACCCGGTGCCGAAGGCGGCGATC 
ATCAGCGCGGTACCGCCGCTGATGGTGAAGACCGAGGGCAATCCGGGCGGCCTGCCGAAGAGCGTCTTCGAC 
GATCTCCAGGCGCAGCTCGCGGCCAATCGCGCGCAGTTCTACCAGGACATTCCCGCCGGCCCGTTCTATGGC 
TACAACCGTCCCGGGGCGCAGCCCTCGGAAGGGATCGTCCGCAACTGGTGGCGGCAGGGCATGATGGGCGGC 
GCCAAGGCCCACTACGATGGGATCGTGGCCTTTTCCCAGACCGACTTCAGCGATGACCTGAAGCGCATCGAC 
ATCCCGGTGCTGGTGATGCATGGCGACGACGACCAGATCGTGCCCTACGAGAATTCCGGCGTACTCTCGGCG 
AAGCTGTTGCGCAACGGCACGCTGAAGATCTATCCGGGGTTCCCGCACGGCATGCCGACCACCCAGGCCGAG 
GTGATCAACGCCGACCTGCTGGCCTTCATCCGCGGCTGA