ViewGene
Locus Name:
GeneID:
Location:

GeneID:265
TrivialName: PA14_06290
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_06290
PAO1 Ortholog Locus:PA0482
Sequence Length:2178
Protein Length:725
Start: 557135
Stop: 554958
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03871.1|AE004485_12 (AE004485) malate synthase G [Pseudomonas aeruginosa PAO1]
Identities = 724/725 (99%), Positives = 724/725 (99%)
Sequence:
ATGACTGAACGCGTTCAAGTCGGTGGCCTGCAGGTCGCCAAAGTCCTGTTCGACTTCGTGAACAACGAAGCC
ATCCCCGGAACCGGCGTCTCTGCCGACACGTTCTGGACCGGTGCGGAGGCCGTGATCAACGACCTGGCGCCG
AAGAATAAAGCCCTGCTCGCCAAGCGCGACGAACTCCAGGCCAAGATCGACGGCTGGCACCAGGCTCGCGCC
GGCCAGGCCCACGACGCCGCTGCCTACAAGGCCTTCCTCGAGGAAATCGGCTACCTGCTGCCCGAGGCGGAA
GACTTCCAGGCCGGCACCCAGAACGTCGACGACGAGATCGCCCGCATGGCCGGCCCGCAGCTGGTGGTGCCG
GTGATGAATGCGCGCTTCGCCCTGAACGCCTCCAACGCCCGCTGGGGCTCGCTGTACGACGCACTCTACGGC
ACCGACGTGATCAGCGAAGAAGGCGGCGCCGAGAAAGGCAAGGGCTACAACAAGGTTCGCGGCGACAAGGTC
ATCGCCTTCGCTCGCGCCTTCCTCGACGAGGCCGCGCCGCTGGAGTCCGGCTCCCATGTCGACGCCACTTCC
TACAGCGTGAAGAACGGCGCGCTGGTCGTCGCGCTGAAGAACGGCAGCGAAACCGGCCTGAAGAACGCTGGC
CAGTTCCTTGCCTTCCAGGGCGACGCCGCCAAGCCGCAAGCGGTACTGCTCAAGCACAACGGCCTGCACTTC
GAAATCCAGATCGACCCGAGCAGCCCGGTCGGCCAGACCGATGCCGCAGGCGTGAAGGACGTGCTGATGGAA
GCCGCCCTCACCACCATCATGGACTGCGAGGACTCGGTCGCCGCGGTCGACGCCGACGACAAGGTAGTGATC
TACCGCAACTGGCTGGGCCTGATGAAAGGCGACCTGGCCGAGGAAGTCAGCAAGGGCGGCAGCACCTTCACC
CGCACCATGAACCCGGACCGCGTCTACACCAGGGCCGACGGTTCCGAACTGACCCTGCACGGCCGCTCGCTG
CTGTTCGTGCGCAACGTCGGCCACTTGATGACCAACGATGCGATCCTCGACAAGGACGGCAACGAAGTGCCG
GAAGGCATCCAGGACGGTCTGTTCACCAGCCTGATCGCCATTCATGACCTGAACGGCAACACCAGCCGCAAG
AACAGCCGCACCGGCAGCGTGTATATCGTCAAGCCGAAGATGCACGGTCCGGAAGAAGCCGCCTTCACCAAC
GAGCTGTTCGGCCGCGTCGAGGACGTCCTCGGCCTGCCGCGCAACACCCTGAAGGTCGGCATCATGGACGAG
GAGCGCCGCACCACCGTGAACCTGAAGGCCTGCATCAAGGCGGCCAAGGATCGCGTGGTGTTCATCAACACC
GGCTTCCTCGACCGCACCGGCGACGAGATCCACACCTCCATGGAAGCCGGCGCCGTGGTGCGCAAGGGCGCC
ATGAAGTCGGAGAAGTGGATCGGCGCCTACGAGAACAACAACGTCGACGTCGGCCTGGCCACCGGCCTGCAA
GGCAAGGCGCAGATCGGCAAGGGCATGTGGGCCATGCCCGACCTGATGGCCGCCATGCTCGAGCAGAAGATC
GGCCATCCGCTGGCCGGCGCCAACACCGCCTGGGTCCCGTCGCCGACCGCCGCGACCCTGCACGCCCTGCAT
TACCACAAGGTCGACGTGTTCGCCCGCCAGGCCGAGCTGGCCAAGCGCACCCCGGCCTCGGTGGACGACATC
CTGACCATTCCGCTGGCGCCGAACACCAACTGGACCGCGGAAGAGATCAAGAACGAGGTGGACAACAACGCC
CAGGGCATCCTCGGCTATGTCGTCCGCTGGATCGACCAGGGCGTCGGCTGCTCCAAGGTGCCGGACATCAAC
GACGTCGGTCTGATGGAAGACCGCGCCACCCTGCGCATCTCCAGCCAGCTGCTGGCCAACTGGCTGCGTCAC
GGCGTGATCAGCCAGGAGCAGGTGGTCGAGAGCCTCAAGCGCATGGCCGTGGTGGTCGACCGGCAGAACGCC
AGCGACCCGTCCTACCGGCCGATGGCGCCGAACTTCGACGACAACGTCGCGTTCCAGGCCGCCCTGGAGCTG
GTGGTGGAAGGCACCCGGCAGCCGAACGGCTATACCGAGCCGGTCCTGCATCGCCGCCGCCGTGAGTTCAAG
GCGAAGAACGGGCTGTAG
Translation:
MTERVQVGGLQVAKVLFDFVNNEAIPGTGVSADTFWTGAEAVINDLAPKNKALLAKRDELQAKIDGWHQARA
GQAHDAAAYKAFLEEIGYLLPEAEDFQAGTQNVDDEIARMAGPQLVVPVMNARFALNASNARWGSLYDALYG
TDVISEEGGAEKGKGYNKVRGDKVIAFARAFLDEAAPLESGSHVDATSYSVKNGALVVALKNGSETGLKNAG
QFLAFQGDAAKPQAVLLKHNGLHFEIQIDPSSPVGQTDAAGVKDVLMEAALTTIMDCEDSVAAVDADDKVVI
YRNWLGLMKGDLAEEVSKGGSTFTRTMNPDRVYTRADGSELTLHGRSLLFVRNVGHLMTNDAILDKDGNEVP
EGIQDGLFTSLIAIHDLNGNTSRKNSRTGSVYIVKPKMHGPEEAAFTNELFGRVEDVLGLPRNTLKVGIMDE
ERRTTVNLKACIKAAKDRVVFINTGFLDRTGDEIHTSMEAGAVVRKGAMKSEKWIGAYENNNVDVGLATGLQ
GKAQIGKGMWAMPDLMAAMLEQKIGHPLAGANTAWVPSPTAATLHALHYHKVDVFARQAELAKRTPASVDDI
LTIPLAPNTNWTAEEIKNEVDNNAQGILGYVVRWIDQGVGCSKVPDINDVGLMEDRATLRISSQLLANWLRH
GVISQEQVVESLKRMAVVVDRQNASDPSYRPMAPNFDDNVAFQAALELVVEGTRQPNGYTEPVLHRRRREFK
AKNGL*
AnnotationID:275GeneID:265
AnnotatorUID: diggins
Modification Date/Time: 2005-03-28 04:04:25
Gene Name:glcB
Confidence Code:2
GeneProduct:malate synthase G
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2225,pfam01274
Secondary Functional Category(ies):energy metabolism
EC Number:4.1.3.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65987.1|AE016227_8 (AE016775) malate synthase [Pseudomonas putida KT2440]
          Length = 725

 Score = 1267 bits (3279), Expect = 0.0
 Identities = 620/725 (85%), Positives = 673/725 (92%)

>gb|AAO54024.1| (AE016857) malate synthase G [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 725

 Score = 1225 bits (3169), Expect = 0.0
 Identities = 597/723 (82%), Positives = 659/723 (91%)

>gb|AAL41078.1| (AE008979) malate synthase G [Agrobacterium tumefaciens str. C58
           (U. Washington)]
          Length = 744

 Score =  997 bits (2578), Expect = 0.0
 Identities = 487/714 (68%), Positives = 587/714 (82%), Gaps = 2/714 (0%)






 
Structural Features:
COG2225, AceB, Malate synthase [Energy production and conversion]
CD-Length = 545 residues,  81.1% aligned
Score =  545 bits (1406), Expect = 6e-156
Identities = 238/475 (50%), Positives = 283/475 (59%), Gaps = 37/475 (7%)

pfam01274, Malate synthase
CD-Length = 524 residues,  86.3% aligned
Score =  471 bits (1214), Expect = 1e-133
Identities = 169/488 (34%), Positives = 214/488 (43%), Gaps = 63/488 (12%)






   
Genomic Context:






   
Comment:


















    ReferenceID:533




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17675




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35336




  
Author/Investigator(s):
        Christiansen I, Hengstenberg W.





  
Title:
        Cloning and sequencing of two genes from Staphylococcus carnosus coding for glucose-specific PTS and their expression in Escherichia coli K-12.




  
PubMed:
        8602153


MedLine:
        




    
Source:
Mol Gen Genet. 1996 Feb 25;250(3):375-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8602153

















    ReferenceID:35337




  
Author/Investigator(s):
        Molina I, Pellicer MT, Badia J, Aguilar J, Baldoma L.





  
Title:
        Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme.




  
PubMed:
        7925370


MedLine:
        




    
Source:
Eur J Biochem. 1994 Sep 1;224(2):541-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7925370

















    ReferenceID:38077




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:265_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:265
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
443
                           PA0482_tr
                           translation of PA0482
                           725
                           99.86
                           99.86
                           0
                           3725.0
     

     
     
23721
                           gb|AAG03871.1|AE004485_12
                           (AE004485) malate synthase G [Pseudomonas aeruginosa PAO1]
                           725
                           99.86
                           99.86
                           0
                           3725.0
     

     
     
23723
                           emb|CAA72726.1|
                           (Y11998) hypothetical protein [Pseudomonas fluorescens]
                           725
                           85.79
                           92.41
                           0
                           3267.0
     

     
     
23722
                           gb|AAN65987.1|AE016227_8
                           (AE016775) malate synthase [Pseudomonas putida KT2440]
                           725
                           85.51
                           92.82
                           0
                           3279.0
     

     
     
23724
                           gb|AAO54024.1|
                           (AE016857) malate synthase G [Pseudomonas syringae pv. tomato str. DC3000]
                           725
                           82.34
                           91.03
                           0
                           3168.0
     

     






      

          
GC ORFID: 43267How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0482Glimmer Score: 

          
Start: 6358477Stop: 6356300

          
Length: 2178


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA0482 translation of PA0482Homolog Bit Score 1439.0

          
Other Homologs:  t_PA0482 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACTGAACGCGTTCAAGTCGGTGGCCTGCAGGTCGCCAAAGTCCTGTTCGACTTCGTGAACAACGAAGCC 
ATCCCCGGAACCGGCGTCTCTGCCGACACGTTCTGGACCGGTGCGGAGGCCGTGATCAACGACCTGGCGCCG 
AAGAATAAAGCCCTGCTCGCCAAGCGCGACGAACTCCAGGCCAAGATCGACGGCTGGCACCAGGCTCGCGCC 
GGCCAGGCCCACGACGCCGCTGCCTACAAGGCCTTCCTCGAGGAAATCGGCTACCTGCTGCCCGAGGCGGAA 
GACTTCCAGGCCGGCACCCAGAACGTCGACGACGAGATCGCCCGCATGGCCGGCCCGCAGCTGGTGGTGCCG 
GTGATGAATGCGCGCTTCGCCCTGAACGCCTCCAACGCCCGCTGGGGCTCGCTGTACGACGCACTCTACGGC 
ACCGACGTGATCAGCGAAGAAGGCGGCGCCGAGAAAGGCAAGGGCTACAACAAGGTTCGCGGCGACAAGGTC 
ATCGCCTTCGCTCGCGCCTTCCTCGACGAGGCCGCGCCGCTGGAGTCCGGCTCCCATGTCGACGCCACTTCC 
TACAGCGTGAAGAACGGCGCGCTGGTCGTCGCGCTGAAGAACGGCAGCGAAACCGGCCTGAAGAACGCTGGC 
CAGTTCCTTGCCTTCCAGGGCGACGCCGCCAAGCCGCAAGCGGTACTGCTCAAGCACAACGGCCTGCACTTC 
GAAATCCAGATCGACCCGAGCAGCCCGGTCGGCCAGACCGATGCCGCAGGCGTGAAGGACGTGCTGATGGAA 
GCCGCCCTCACCACCATCATGGACTGCGAGGACTCGGTCGCCGCGGTCGACGCCGACGACAAGGTAGTGATC 
TACCGCAACTGGCTGGGCCTGATGAAAGGCGACCTGGCCGAGGAAGTCAGCAAGGGCGGCAGCACCTTCACC 
CGCACCATGAACCCGGACCGCGTCTACACCAGGGCCGACGGTTCCGAACTGACCCTGCACGGCCGCTCGCTG 
CTGTTCGTGCGCAACGTCGGCCACTTGATGACCAACGATGCGATCCTCGACAAGGACGGCAACGAAGTGCCG 
GAAGGCATCCAGGACGGTCTGTTCACCAGCCTGATCGCCATTCATGACCTGAACGGCAACACCAGCCGCAAG 
AACAGCCGCACCGGCAGCGTGTATATCGTCAAGCCGAAGATGCACGGTCCGGAAGAAGCCGCCTTCACCAAC 
GAGCTGTTCGGCCGCGTCGAGGACGTCCTCGGCCTGCCGCGCAACACCCTGAAGGTCGGCATCATGGACGAG 
GAGCGCCGCACCACCGTGAACCTGAAGGCCTGCATCAAGGCGGCCAAGGATCGCGTGGTGTTCATCAACACC 
GGCTTCCTCGACCGCACCGGCGACGAGATCCACACCTCCATGGAAGCCGGCGCCGTGGTGCGCAAGGGCGCC 
ATGAAGTCGGAGAAGTGGATCGGCGCCTACGAGAACAACAACGTCGACGTCGGCCTGGCCACCGGCCTGCAA 
GGCAAGGCGCAGATCGGCAAGGGCATGTGGGCCATGCCCGACCTGATGGCCGCCATGCTCGAGCAGAAGATC 
GGCCATCCGCTGGCCGGCGCCAACACCGCCTGGGTCCCGTCGCCGACCGCCGCGACCCTGCACGCCCTGCAT 
TACCACAAGGTCGACGTGTTCGCCCGCCAGGCCGAGCTGGCCAAGCGCACCCCGGCCTCGGTGGACGACATC 
CTGACCATTCCGCTGGCGCCGAACACCAACTGGACCGCGGAAGAGATCAAGAACGAGGTGGACAACAACGCC 
CAGGGCATCCTCGGCTATGTCGTCCGCTGGATCGACCAGGGCGTCGGCTGCTCCAAGGTGCCGGACATCAAC 
GACGTCGGTCTGATGGAAGACCGCGCCACCCTGCGCATCTCCAGCCAGCTGCTGGCCAACTGGCTGCGTCAC 
GGCGTGATCAGCCAGGAGCAGGTGGTCGAGAGCCTCAAGCGCATGGCCGTGGTGGTCGACCGGCAGAACGCC 
AGCGACCCGTCCTACCGGCCGATGGCGCCGAACTTCGACGACAACGTCGCGTTCCAGGCCGCCCTGGAGCTG 
GTGGTGGAAGGCACCCGGCAGCCGAACGGCTATACCGAGCCGGTCCTGCATCGCCGCCGCCGTGAGTTCAAG 
GCGAAGAACGGGCTGTAG