ViewGene
Locus Name:
GeneID:
Location:

GeneID:2649
TrivialName: PA14_22330
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_22330
PAO1 Ortholog Locus:PA3236
Sequence Length:858
Protein Length:285
Start: 1944551
Stop: 1945408
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06624.1|AE004746_8 (AE004746) probable glycine betaine-binding protein precursor [Pseudomonas aeruginosa PAO1]
Identities = 285/285 (100%), Positives = 285/285 (100%)
Sequence:
ATGCGAATGATGCGACGACTGTTGTGCTGGAGTGCGGGCCTGGCAATGTCGGCGGCGGTGGGGATGGCGGCC
GCGGCGGACAAGCCGGTGATCACCATCGGTTACGTGGACGGCTGGTCGGACAGCGTGGCGACCACCCATGTG
GCCGCCGAGGTGATCCGCGAGAAACTCGGCTACGAGGTGAAGCTGATGCCGGTGGCGGCGGGGATCATGTGG
CAGGGCGTGGCGCGCGGCAAGCTCGATGCCATGCTCTCGGCCTGGCTGCCGGGCACCCACGGCGCCTACTAC
GAGAAGATGAAGGACAAGGTGGTCAACCTTGCCATCAACTACCCGGACGCACGCATCGGCCTGATCGTCCCC
GAATACGTGAGCGCCAACAGCATCGCCGACCTCCAGGCGCAGAAGGACGCTTTCGGCGGCCGCGTGGTGGGC
ATCGATGCCGGCGCCGGGGTGATGATCAAGACCGACGAGGCGATCAAGCAATATGGCCTCGACTACAAGCTG
GTGGCCAGTTCCGGCAGCGGCATGATCGCCGAGCTGACCCGTGCCGAGAACGAGAAGAAGCCGGTGGTGGTG
ACCGGCTGGATTCCGCACTGGATGTTCGCCAAGTGGAAGCTGAAGTTCCTCGACGATCCGAAGAAGGTCTAT
GGCGATACCGAGCATGTCGACACCGTCGCCAACCCGGCGCTGGAGGCCAAGGCCAAGCCGGTCTGGGAATTC
CTGAAGAAGTTCGGCTGGAAGGATGGCAACGAGGTTGGCCAGGTGATGCTGGCGATCCAGGAGGGCGCCAAA
CCCGAGGATGCGGCGAAGCAGTGGGTGGCGGCCAACCCGGGACGGGTCAAGGACTGGGTGCAGTAG
Translation:
MRMMRRLLCWSAGLAMSAAVGMAAAADKPVITIGYVDGWSDSVATTHVAAEVIREKLGYEVKLMPVAAGIMW
QGVARGKLDAMLSAWLPGTHGAYYEKMKDKVVNLAINYPDARIGLIVPEYVSANSIADLQAQKDAFGGRVVG
IDAGAGVMIKTDEAIKQYGLDYKLVASSGSGMIAELTRAENEKKPVVVTGWIPHWMFAKWKLKFLDDPKKVY
GDTEHVDTVANPALEAKAKPVWEFLKKFGWKDGNEVGQVMLAIQEGAKPEDAAKQWVAANPGRVKDWVQ*
AnnotationID:2657GeneID:2649
AnnotatorUID: diggins
Modification Date/Time: 2005-04-14 13:01:02
Gene Name:
Confidence Code:3
GeneProduct:putative glycine betaine-binding protein precursor
Cell Localization:()
Synonyms:PA3236
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:ABC-type proline/glycine betaine transport systems, periplasmic component
Functional Category Confidence Code:5
COGs:pfam04069,COG2113
Secondary Functional Category(ies):adaptation, protection
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55142.1| (AE016861) glycine betaine-binding protein, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 328

 Score =  361 bits (926), Expect = 3e-99
 Identities = 184/282 (65%), Positives = 217/282 (76%), Gaps = 3/282 (1%)

>emb|CAD17215.1| (AL646076) PUTATIVE GLYCINE BETAINE TRANSMEMBRANE AND PERIPLASMIC
           ABC TRANSPORTER PROTEIN [Ralstonia solanacearum]
          Length = 289

 Score =  361 bits (926), Expect = 3e-99
 Identities = 178/285 (62%), Positives = 213/285 (74%), Gaps = 1/285 (0%)

>gb|AAN67361.1|AE016362_5 (AE016780) glycine betaine-binding protein, putative [Pseudomonas
           putida KT2440]
          Length = 287

 Score =  350 bits (897), Expect = 7e-96
 Identities = 177/284 (62%), Positives = 217/284 (76%), Gaps = 5/284 (1%)








 
Structural Features:
pfam04069, OpuAC, Substrate binding domain of ABC-type 
glycine betaine transport system.
CD-Length = 260 residues,  99.2% aligned
Score =  195 bits (498), Expect = 4e-51
Identities = 91/264 (34%), Positives = 134/264 (50%), Gaps = 15/264 (5%)

COG2113, ProX, ABC-type proline/glycine betaine transport systems, 
periplasmic components 
[Amino acid transport and metabolism]
CD-Length = 302 residues,  97.7% aligned
Score =  247 bits (631), Expect = 1e-66
Identities = 119/296 (40%), Positives = 162/296 (54%), Gaps = 12/296 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5301




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22446




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32307




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2649_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2649
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4366
                           PA3236_tr
                           translation of PA3236
                           285
                           99.99
                           99.99
                           0
                           1493.0
     

     
     
127562
                           gb|AAG06624.1|AE004746_8
                           (AE004746) probable glycine betaine-binding protein precursor [Pseudomonas aeruginosa PAO1]
                           285
                           99.99
                           99.99
                           0
                           1493.0
     

     
     
127565
                           gb|AAN67361.1|AE016362_5
                           (AE016780) glycine betaine-binding protein, putative [Pseudomonas putida KT2440]
                           293
                           61.77
                           74.40
                           14
                           902.0
     

     
     
127564
                           emb|CAD17215.1|
                           (AL646076) PUTATIVE GLYCINE BETAINE TRANSMEMBRANE AND PERIPLASMIC ABC TRANSPORTER PROTEIN [Ralstonia solanacearum]
                           290
                           61.37
                           73.44
                           6
                           928.0
     

     
     
127563
                           gb|AAO55142.1|
                           (AE016861) glycine betaine-binding protein, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           331
                           56.19
                           65.86
                           49
                           932.0
     

     






      

          
GC ORFID: 45651How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3236Glimmer Score: 

          
Start: 3009380Stop: 3008523

          
Length: 858


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3236 translation of PA3236Homolog Bit Score 579.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6389 (ORF: BLASTX 363.0), t_PA3236 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGAATGATGCGACGACTGTTGTGCTGGAGTGCGGGCCTGGCAATGTCGGCGGCGGTGGGGATGGCGGCC 
GCGGCGGACAAGCCGGTGATCACCATCGGTTACGTGGACGGCTGGTCGGACAGCGTGGCGACCACCCATGTG 
GCCGCCGAGGTGATCCGCGAGAAACTCGGCTACGAGGTGAAGCTGATGCCGGTGGCGGCGGGGATCATGTGG 
CAGGGCGTGGCGCGCGGCAAGCTCGATGCCATGCTCTCGGCCTGGCTGCCGGGCACCCACGGCGCCTACTAC 
GAGAAGATGAAGGACAAGGTGGTCAACCTTGCCATCAACTACCCGGACGCACGCATCGGCCTGATCGTCCCC 
GAATACGTGAGCGCCAACAGCATCGCCGACCTCCAGGCGCAGAAGGACGCTTTCGGCGGCCGCGTGGTGGGC 
ATCGATGCCGGCGCCGGGGTGATGATCAAGACCGACGAGGCGATCAAGCAATATGGCCTCGACTACAAGCTG 
GTGGCCAGTTCCGGCAGCGGCATGATCGCCGAGCTGACCCGTGCCGAGAACGAGAAGAAGCCGGTGGTGGTG 
ACCGGCTGGATTCCGCACTGGATGTTCGCCAAGTGGAAGCTGAAGTTCCTCGACGATCCGAAGAAGGTCTAT 
GGCGATACCGAGCATGTCGACACCGTCGCCAACCCGGCGCTGGAGGCCAAGGCCAAGCCGGTCTGGGAATTC 
CTGAAGAAGTTCGGCTGGAAGGATGGCAACGAGGTTGGCCAGGTGATGCTGGCGATCCAGGAGGGCGCCAAA 
CCCGAGGATGCGGCGAAGCAGTGGGTGGCGGCCAACCCGGGACGGGTCAAGGACTGGGTGCAGTAG