ViewGene
Locus Name:
GeneID:
Location:

GeneID:2637
TrivialName: PA14_66830
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66830
PAO1 Ortholog Locus:PA5057
Sequence Length:858
Protein Length:285
Start: 5968582
Stop: 5969439
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08442.1|AE004919_3 (AE004919) poly(3-hydroxyalkanoic acid) depolymerase [Pseudomonas aeruginosa PAO1]
Identities = 285/285 (100%), Positives = 285/285 (100%)
Sequence:
ATGCCCCAGCCCTTCGTCTTCCGGACCATCGACCTCGACGGCCAGACCATCCGCACCGCAGTGCGGCCGGGC
AAGGAAGGAAGCACTCCGCTGCTGATCTTCAACGGCATAGGCGCCAACCTGGAACTGGTATTCCCCTTCGTC
CAGGCGCTCGACCCGGAACTGGAGGTGATCGCCTTCGACGTTCCCGGCGTCGGCGGTTCCTCGACGCCCAGC
GTGCCCTACCGCTTTCCCGGCCTGGCCAAGCTGGCGGCGCGGATGCTCGACTACCTGGACTACGGCCAGGTC
AACGCGATCGGCGTGTCCTGGGGCGGCGCGCTGGCCCAGCAGTTCGCCCACGACTATCCGGAACGCTGCAAG
AAGCTGATCCTCGCCGCCACTTCGGCTGGCGCGGTGATGGTGCCAGGCAAGCCGAAGGTACTGATGCGCATG
GCCAGCCCGCGGCGCTACATCCAGCCCTCCTATGGCGTACACATCGCACCGGACATCTACGGCGGGGCCTTC
CGCCGCGACCCCAAGCTGGCCATGGCGCATGCCAGCAAGGTGCGTTCGTCGGGCAAGCTGGGCTACTACTGG
CAATTGTTCGCCGGGCTCGGCTGGACCAGCATCCACTGGCTGCATAGGATCCGCCAGCCGACCCTGGTGCTG
GCCGGCGACGACGACCCGATCATCCCGCTGATCAACATGCGCGTGCTGGCCTGGCGCATTCCCAACGCCGAA
CTGCACGTGATCGACGACGGCCACCTGTTCCTGGTGACCCGCGCCGAATCGGTGGCGCCGATCATCATGAAG
TTCCTCGCCGAGGAGCGCCGCCGCGCCGTCATGCACCCTCGTCCGTTCCTGCCCAAGACCGGCTGA
Translation:
MPQPFVFRTIDLDGQTIRTAVRPGKEGSTPLLIFNGIGANLELVFPFVQALDPELEVIAFDVPGVGGSSTPS
VPYRFPGLAKLAARMLDYLDYGQVNAIGVSWGGALAQQFAHDYPERCKKLILAATSAGAVMVPGKPKVLMRM
ASPRRYIQPSYGVHIAPDIYGGAFRRDPKLAMAHASKVRSSGKLGYYWQLFAGLGWTSIHWLHRIRQPTLVL
AGDDDPIIPLINMRVLAWRIPNAELHVIDDGHLFLVTRAESVAPIIMKFLAEERRRAVMHPRPFLPKTG*
AnnotationID:2645GeneID:2637
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 11:11:41
Gene Name:phaD
Confidence Code:1
GeneProduct:poly(3-hydroxyalkanoic acid) depolymerase
Cell Localization:()
Synonyms:phaZ,phaB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00561,COG0596
Secondary Functional Category(ies):Secondary metabolites biosynthesis, transport, and catabolism
EC Number:3.1.1.76
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK19607.2|AF336849_1 (AF336849) PHA depolymerase [Pseudomonas nitroreducens]
          Length = 285

 Score =  531 bits (1369), Expect = e-150
 Identities = 257/279 (92%), Positives = 266/279 (95%)

>gb|AAP33694.1| (AY278219) PHA depolymerase [Pseudomonas stutzeri]
          Length = 285

 Score =  530 bits (1365), Expect = e-150
 Identities = 256/279 (91%), Positives = 267/279 (95%)

>gb|AAK81718.1| (AF394660) PHA depolymerase [Burkholderia caryophylli]
          Length = 283

 Score =  513 bits (1322), Expect = e-145
 Identities = 244/279 (87%), Positives = 265/279 (94%)








 
Structural Features:
pfam00561, Abhydrolase_1, alpha/beta hydrolase fold.
CD-Length = 224 residues,  99.6% aligned
Score =  119 bits (298), Expect = 5e-28
Identities = 75/223 (33%), Positives = 104/223 (46%), Gaps = 14/223 (6%)

COG0596, MhpC, Predicted hydrolases or acyltransferases 
(alpha/beta hydrolase superfamily)
CD-Length = 282 residues,  99.3% aligned
Score = 87.8 bits (215), Expect = 1e-18







   
Genomic Context:






   
Comment:


















    ReferenceID:5277




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22422




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35012




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2637_cdd.html


    		




 
URL:

















    ReferenceID:35831




  
Author/Investigator(s):
        Timm A, Steinbuchel A.





  
Title:
        Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1.




  
PubMed:
        1396693


MedLine:
        




    
Source:
Eur J Biochem. 1992 Oct 1;209(1):15-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=93011120&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:2637
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4353
                           PA5057_tr
                           translation of PA5057
                           285
                           99.99
                           99.99
                           0
                           1497.0
     

     
     
127312
                           gb|AAG08442.1|AE004919_3
                           (AE004919) poly(3-hydroxyalkanoic acid) depolymerase [Pseudomonas aeruginosa PAO1]
                           285
                           99.99
                           99.99
                           0
                           1497.0
     

     
     
127313
                           emb|CAA47152.1|
                           (X66592) PHA-depolymerase [Pseudomonas aeruginosa]
                           285
                           99.99
                           99.99
                           0
                           1497.0
     

     
     
127314
                           gb|AAQ72542.1|
                           (AY370936) MCL-PHA extracellular depolymerase [Pseudomonas sp. HJ-2]
                           285
                           90.87
                           94.73
                           2
                           1375.0
     

     
     
127315
                           gb|AAK19607.2|AF336849_1
                           (AF336849) PHA depolymerase [Pseudomonas nitroreducens]
                           285
                           90.17
                           93.68
                           0
                           1361.0
     

     






      

          
GC ORFID: 45639How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5057Glimmer Score: 

          
Start: 5232265Stop: 5233122

          
Length: 858


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5057 translation of PA5057Homolog Bit Score 581.0

          
Other Homologs:  t_PA5057 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCCAGCCCTTCGTCTTCCGGACCATCGACCTCGACGGCCAGACCATCCGCACCGCAGTGCGGCCGGGC 
AAGGAAGGAAGCACTCCGCTGCTGATCTTCAACGGCATAGGCGCCAACCTGGAACTGGTATTCCCCTTCGTC 
CAGGCGCTCGACCCGGAACTGGAGGTGATCGCCTTCGACGTTCCCGGCGTCGGCGGTTCCTCGACGCCCAGC 
GTGCCCTACCGCTTTCCCGGCCTGGCCAAGCTGGCGGCGCGGATGCTCGACTACCTGGACTACGGCCAGGTC 
AACGCGATCGGCGTGTCCTGGGGCGGCGCGCTGGCCCAGCAGTTCGCCCACGACTATCCGGAACGCTGCAAG 
AAGCTGATCCTCGCCGCCACTTCGGCTGGCGCGGTGATGGTGCCAGGCAAGCCGAAGGTACTGATGCGCATG 
GCCAGCCCGCGGCGCTACATCCAGCCCTCCTATGGCGTACACATCGCACCGGACATCTACGGCGGGGCCTTC 
CGCCGCGACCCCAAGCTGGCCATGGCGCATGCCAGCAAGGTGCGTTCGTCGGGCAAGCTGGGCTACTACTGG 
CAATTGTTCGCCGGGCTCGGCTGGACCAGCATCCACTGGCTGCATAGGATCCGCCAGCCGACCCTGGTGCTG 
GCCGGCGACGACGACCCGATCATCCCGCTGATCAACATGCGCGTGCTGGCCTGGCGCATTCCCAACGCCGAA 
CTGCACGTGATCGACGACGGCCACCTGTTCCTGGTGACCCGCGCCGAATCGGTGGCGCCGATCATCATGAAG 
TTCCTCGCCGAGGAGCGCCGCCGCGCCGTCATGCACCCTCGTCCGTTCCTGCCCAAGACCGGCTGA