ViewGene
Locus Name:
GeneID:
Location:

GeneID:2628
TrivialName: PA14_10800
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10800
PAO1 Ortholog Locus:PA4109
Sequence Length:891
Protein Length:296
Start: 933973
Stop: 934863
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07496.1|AE004827_5 (AE004827) transcriptional regulator AmpR [Pseudomonas aeruginosa PAO1]
Identities = 293/296 (98%), Positives = 293/296 (98%)
Sequence:
TTGGTTCGACCCCATTTGCCGCTGAACGCCCTGCGCGCCTTCGAAGCTTCGGCCCGGCACCTGAGCTTCACC
CGCGCGGCCATCGAGCTGTGCGTGACCCAGGCGGCGGTCAGCCACCAGGTGAAGAGCCTCGAGGAGCGTCTC
GGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTG
CTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTC
ACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCAT
CCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAGGGGCTCGACTACGCG
ATCCGCTTCGGCGGCGGCGCCTGGCACGGCACCGAGGCGCTGGCGTTGTTCGAGGCGCCGCTGACGGTGCTC
TGCTGCCCGGAGGTCGCCGCCCAGTTGCACAGTCCCGCCGACCTGCTGCAGCACACCCTGCTGCGCTCCTAC
CGCGCCGACGAGTGGCCGCTGTGGTTCCAGGCGGCCGGACTGCCGGCGCACGCGCCACTGACCCGGAGCATC
GTCTTCGACACCTCGCTGGCCATGCTCGAGGCGGCCCGCCAGGGTGTCGGCGTGGCCCTGGCGCCGGCGGCG
ATGTTTGCCCGGCAACTGGCCAGCGAGAGCATCCGGCGTCCGTTCGCCACCGAAGTGAGTACCGGCAGCTAC
TGGCTGACGCGCTTGCAGTCGCGGGGGGAGACCAGCGCGATGCTGGCGTTCAGGGAGTGGTTGCTGGAGAGG
GCTGCCGTTGAGGCGCGGGGGAGATAA
Translation:
MVRPHLPLNALRAFEASARHLSFTRAAIELCVTQAAVSHQVKSLEERLGVALFKRLPRGLMLTHEGESLLPV
LCDSFDRIAGLLERFEGGHYRDVLTVGAVGTFTVGWLLPRLADFQARHPFIDLRLSTHNNRVDIAAEGLDYA
IRFGGGAWHGTEALALFEAPLTVLCCPEVAAQLHSPADLLQHTLLRSYRADEWPLWFQAAGLPAHAPLTRSI
VFDTSLAMLEAARQGVGVALAPAAMFARQLASESIRRPFATEVSTGSYWLTRLQSRGETSAMLAFREWLLER
AAVEARGR*
AnnotationID:2636GeneID:2628
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 11:11:17
Gene Name:ampR
Confidence Code:1
GeneProduct:transcriptional regulator AmpR
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0583,pfam03466,pfam00126
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAC17148.1| (AJ299421) transcriptional activator [Ochrobactrum anthropi]
          Length = 291

 Score =  438 bits (1126), Expect = e-122
 Identities = 217/289 (75%), Positives = 244/289 (84%)

>gb|AAL43894.1| (AE009239) transcriptional regulator, LysR family [Agrobacterium
           tumefaciens str. C58 (U. Washington)]
          Length = 292

 Score =  394 bits (1013), Expect = e-109
 Identities = 196/287 (68%), Positives = 231/287 (80%)

>gb|AAM93474.1| (AY125471) AmpR transcriptional regulator [Enterobacter cloacae]
          Length = 291

 Score =  362 bits (929), Expect = 1e-99
 Identities = 183/288 (63%), Positives = 218/288 (75%)






 
Structural Features:
COG0583, LysR, Transcriptional regulator [Transcription]
CD-Length = 297 residues,  99.7% aligned
Score =  128 bits (323), Expect = 5e-31
Identities = 94/296 (31%), Positives = 129/296 (43%), Gaps = 7/296 (2%)

pfam03466, LysR_substrate, LysR substrate binding domain
CD-Length = 209 residues, 100.0% aligned
Score = 92.8 bits (230), Expect = 4e-20
Identities = 61/209 (29%), Positives = 91/209 (43%), Gaps = 7/209 (3%)

pfam00126, HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family.
CD-Length = 60 residues, 100.0% aligned
Score = 76.3 bits (188), Expect = 4e-15
Identities = 36/60 (60%), Positives = 41/60 (68%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14641




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22404




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36842




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2628_cdd.html


    		




 
URL:

















    ReferenceID:36950




  
Author/Investigator(s):
        Lodge J, Busby S, Piddock L.





  
Title:
        Investigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC beta-lactamase promoter.




  
PubMed:
        8405939


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1993 Aug 1;111(2-3):315-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8405939









           
Homologs By Global Alignment 

           

       



     
Gene ID:2628
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4344
                           PA4109_tr
                           translation of PA4109
                           296
                           98.98
                           98.98
                           0
                           1501.0
     

     
     
127168
                           gb|AAG07496.1|AE004827_5
                           (AE004827) transcriptional regulator AmpR [Pseudomonas aeruginosa PAO1]
                           296
                           98.64
                           98.98
                           0
                           1499.0
     

     
     
127169
                           emb|CAA47470.1|
                           (X67095) AmpR B-lactamase regulatory protein [Pseudomonas aeruginosa]
                           296
                           97.97
                           98.31
                           0
                           1489.0
     

     
     
127170
                           emb|CAC17148.1|
                           (AJ299421) transcriptional activator [Ochrobactrum anthropi]
                           296
                           72.97
                           82.43
                           5
                           1118.0
     

     
     
127171
                           emb|CAC04521.1|
                           (AJ401618) AmpR transcriptional activator [Ochrobactrum anthropi]
                           296
                           72.97
                           82.09
                           5
                           1116.0
     

     






      

          
GC ORFID: 45630How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4109Glimmer Score: 

          
Start: 4019964Stop: 4019074

          
Length: 891


          
Start Codon: TTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4109 translation of PA4109Homolog Bit Score 582.0

          
Other Homologs:  t_PA4109 (ORF: GLIMMER)

          
GC ORF Sequence

           
TTGGTTCGACCCCATTTGCCGCTGAACGCCCTGCGCGCCTTCGAAGCTTCGGCCCGGCACCTGAGCTTCACC 
CGCGCGGCCATCGAGCTGTGCGTGACCCAGGCGGCGGTCAGCCACCAGGTGAAGAGCCTCGAGGAGCGTCTC 
GGCGTGGCCCTGTTCAAGCGTCTGCCGCGCGGCCTGATGCTGACCCACGAGGGCGAGAGCCTGCTGCCGGTG 
CTGTGTGACTCCTTCGACCGCATCGCCGGCCTGCTGGAACGCTTCGAGGGCGGCCACTACCGGGACGTGCTC 
ACCGTCGGCGCGGTCGGAACCTTCACGGTCGGCTGGCTGCTGCCGCGGCTGGCGGACTTCCAGGCGCGCCAT 
CCCTTCATCGATCTGCGCCTGTCCACCCACAACAACCGCGTCGACATCGCCGCCGAGGGGCTCGACTACGCG 
ATCCGCTTCGGCGGCGGCGCCTGGCACGGCACCGAGGCGCTGGCGTTGTTCGAGGCGCCGCTGACGGTGCTC 
TGCTGCCCGGAGGTCGCCGCCCAGTTGCACAGTCCCGCCGACCTGCTGCAGCACACCCTGCTGCGCTCCTAC 
CGCGCCGACGAGTGGCCGCTGTGGTTCCAGGCGGCCGGACTGCCGGCGCACGCGCCACTGACCCGGAGCATC 
GTCTTCGACACCTCGCTGGCCATGCTCGAGGCGGCCCGCCAGGGTGTCGGCGTGGCCCTGGCGCCGGCGGCG 
ATGTTTGCCCGGCAACTGGCCAGCGAGAGCATCCGGCGTCCGTTCGCCACCGAAGTGAGTACCGGCAGCTAC 
TGGCTGACGCGCTTGCAGTCGCGGGGGGAGACCAGCGCGATGCTGGCGTTCAGGGAGTGGTTGCTGGAGAGG 
GCTGCCGTTGAGGCGCGGGGGAGATAA