ViewGene
Locus Name:
GeneID:
Location:

GeneID:2625
TrivialName: PA14_05590
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05590
PAO1 Ortholog Locus:PA0430
Sequence Length:873
Protein Length:290
Start: 496819
Stop: 495947
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03819.1|AE004480_3 (AE004480) 5,10-methylenetetrahydrofolate reductase [Pseudomonas aeruginosa PAO1]
Identities = 290/290 (100%), Positives = 290/290 (100%)
Sequence:
GTGGTCGCGTCCAAGGAACCGATCATGAGTCAGAGCGAACGCCGTTTCAGCTTCGAGTTCTTCCCGGCGAAG
ACCGAGGCCGGCCATGAAAAGCTGTTGGCCACCGCCCGCAACCTGGCGGGCTACAAGCCCGACTTCTTCTCC
TGCACCTACGGCGCCGGCGGATCCACCCGCGACCGCACGTTGAGTACCGTGCTGCAACTGGACGGCGAGGTG
AAAGTGCCGACCGCGCCCCACCTGTCCTGTGTCGGCGACTCGAAAGCCGAGTTGCGCGAACTGCTCGGCCGC
TACCGCGAGGCCGGCATCCGCCGCATCGTCGCCCTGCGCGGCGACCTGCCGTCGGGCATGGGCATGGCCAGC
GGCGAACTGCGCTACGCCAACGAACTGGTGGACTTCATCCGCACCGAGACCGGCGACCACTTCCACATCGAG
GTCGCCGCCTATCCGGAAGTCCACCCCCAGGCGCGCAGCTTCGAGGATGACCTGGCGAACTTCGTGCGCAAG
GTGAAGGCCGGCGCCAGCAGCGCCATCACCCAGTACTTCTTCAACGCCGATGCCTATTTCTACTTCGTCGAG
CGGGTCGCCAAGCTCGGCGTGGACATCCCGGTGGTCCCCGGCATCATGCCGATCACCAACTACTCCAAGCTG
GCGCGCTTCTCCGACGCCTGCGGCGCCGAACTGCCGCGCTGGATCCGCAAGCAACTGGAAGCCTACGGCGAC
GACAGCCGCAGCATCCAGGCCTTCGGCGAGCAGGTCATCAGCGAGATGTGCGAACGCCTGCTGGAGGGCGGC
GCACCGGGACTGCATTTCTATACTTTGAACCAGGCCGATCCGAGCCTGGCGATCTGGAAGAATCTCCAGCTG
CCACGCTGA
Translation:
MVASKEPIMSQSERRFSFEFFPAKTEAGHEKLLATARNLAGYKPDFFSCTYGAGGSTRDRTLSTVLQLDGEV
KVPTAPHLSCVGDSKAELRELLGRYREAGIRRIVALRGDLPSGMGMASGELRYANELVDFIRTETGDHFHIE
VAAYPEVHPQARSFEDDLANFVRKVKAGASSAITQYFFNADAYFYFVERVAKLGVDIPVVPGIMPITNYSKL
ARFSDACGAELPRWIRKQLEAYGDDSRSIQAFGEQVISEMCERLLEGGAPGLHFYTLNQADPSLAIWKNLQL
PR*
AnnotationID:2633GeneID:2625
AnnotatorUID: diggins
Modification Date/Time: 2004-08-11 17:05:57
Gene Name:metF
Confidence Code:2
GeneProduct:5,10-methylenetetrahydrofolate reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam02219,COG0685
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70543.1|AE016696_8 (AE016792) 5,10-methylenetetrahydrofolate reductase [Pseudomonas
           putida KT2440]
          Length = 295

 Score =  486 bits (1251), Expect = e-137
 Identities = 234/286 (81%), Positives = 261/286 (91%), Gaps = 1/286 (0%)

>gb|AAO58496.1| (AE016874) 5,10-methylenetetrahydrofolate reductase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 281

 Score =  484 bits (1245), Expect = e-136
 Identities = 235/282 (83%), Positives = 256/282 (90%), Gaps = 1/282 (0%)

>emb|CAD13619.1| (AL646057) PROBABLE 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE
           OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum]
          Length = 276

 Score =  355 bits (912), Expect = 1e-97
 Identities = 178/276 (64%), Positives = 211/276 (76%), Gaps = 2/276 (0%)






 
Structural Features:
pfam02219, MTHFR, Methylenetetrahydrofolate reductase.

COG0685, MetF, 5,10-methylenetetrahydrofolate reductase 
[Amino acid transport and metabolism]






   
Genomic Context:






   
Comment:


















    ReferenceID:14639




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22398




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33755




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2625_cdd.html


    		




 
URL:

















    ReferenceID:35355




  
Author/Investigator(s):
        Saint-Girons I, Duchange N, Zakin MM, Park I, Margarita D, Ferrara P, Cohen GN.





  
Title:
        Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.




  
PubMed:
        6356036


MedLine:
        




    
Source:
Nucleic Acids Res. 1983 Oct 11;11(19):6723-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6356036

















    ReferenceID:35356




  
Author/Investigator(s):
        Belfaiza J, Guillou Y, Margarita D, Perrin D, Saint Girons I.





  
Title:
        Operator-constitutive mutations of the Escherichia coli metF gene.




  
PubMed:
        3542965


MedLine:
        




    
Source:
J Bacteriol. 1987 Feb;169(2):670-4.

Erratum in:
    * J Bacteriol 1987 Jun;169(6):2911.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3542965

















    ReferenceID:35357




  
Author/Investigator(s):
        Stauffer GV, Stauffer LT.





  
Title:
        Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli.




  
PubMed:
        2841568


MedLine:
        




    
Source:
Mol Gen Genet. 1988 May;212(2):246-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2841568

















    ReferenceID:35358




  
Author/Investigator(s):
        Bourhy P, Saint Girons I.





  
Title:
        Localization of the Leptospira interrogans metF gene on the CII secondary chromosome.




  
PubMed:
        11024273


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2000 Oct 15;191(2):259-63.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11024273









           
Homologs By Global Alignment 

           

       



     
Gene ID:2625
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4340
                           PA0430_tr
                           translation of PA0430
                           290
                           99.99
                           99.99
                           0
                           1502.0
     

     
     
127072
                           gb|AAG03819.1|AE004480_3
                           (AE004480) 5,10-methylenetetrahydrofolate reductase [Pseudomonas aeruginosa PAO1]
                           290
                           99.65
                           99.99
                           0
                           1499.0
     

     
     
127074
                           gb|AAO58496.1|
                           (AE016874) 5,10-methylenetetrahydrofolate reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           290
                           81.03
                           88.27
                           9
                           1247.0
     

     
     
127073
                           gb|AAN70543.1|AE016696_8
                           (AE016792) 5,10-methylenetetrahydrofolate reductase [Pseudomonas putida KT2440]
                           296
                           79.05
                           88.51
                           7
                           1249.0
     

     
     
127075
                           gb|AAQ58640.1|
                           (AE016913) 5,10-methylenetetrahydrofolate reductase [Chromobacterium violaceum ATCC 12472]
                           290
                           62.06
                           76.20
                           12
                           951.0
     

     






      

          
GC ORFID: 45627How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0430Glimmer Score: 

          
Start: 6298161Stop: 6297289

          
Length: 873


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0430 translation of PA0430Homolog Bit Score 583.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3734 (ORF: BLASTX 481.0), t_PA0430 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGTCGCGTCCAAGGAACCGATCATGAGTCAGAGCGAACGCCGTTTCAGCTTCGAGTTCTTCCCGGCGAAG 
ACCGAGGCCGGCCATGAAAAGCTGTTGGCCACCGCCCGCAACCTGGCGGGCTACAAGCCCGACTTCTTCTCC 
TGCACCTACGGCGCCGGCGGATCCACCCGCGACCGCACGTTGAGTACCGTGCTGCAACTGGACGGCGAGGTG 
AAAGTGCCGACCGCGCCCCACCTGTCCTGTGTCGGCGACTCGAAAGCCGAGTTGCGCGAACTGCTCGGCCGC 
TACCGCGAGGCCGGCATCCGCCGCATCGTCGCCCTGCGCGGCGACCTGCCGTCGGGCATGGGCATGGCCAGC 
GGCGAACTGCGCTACGCCAACGAACTGGTGGACTTCATCCGCACCGAGACCGGCGACCACTTCCACATCGAG 
GTCGCCGCCTATCCGGAAGTCCACCCCCAGGCGCGCAGCTTCGAGGATGACCTGGCGAACTTCGTGCGCAAG 
GTGAAGGCCGGCGCCAGCAGCGCCATCACCCAGTACTTCTTCAACGCCGATGCCTATTTCTACTTCGTCGAG 
CGGGTCGCCAAGCTCGGCGTGGACATCCCGGTGGTCCCCGGCATCATGCCGATCACCAACTACTCCAAGCTG 
GCGCGCTTCTCCGACGCCTGCGGCGCCGAACTGCCGCGCTGGATCCGCAAGCAACTGGAAGCCTACGGCGAC 
GACAGCCGCAGCATCCAGGCCTTCGGCGAGCAGGTCATCAGCGAGATGTGCGAACGCCTGCTGGAGGGCGGC 
GCACCGGGACTGCATTTCTATACTTTGAACCAGGCCGATCCGAGCCTGGCGATCTGGAAGAATCTCCAGCTG 
CCACGCTGA