ViewGene
Locus Name:
GeneID:
Location:

GeneID:2620
TrivialName: PA14_47500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47500
PAO1 Ortholog Locus:PA1292
Sequence Length:855
Protein Length:284
Start: 4230769
Stop: 4231623
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04681.1|AE004558_10 (AE004558) probable 3-mercaptopyruvate sulfurtransferase [Pseudomonas aeruginosa PAO1]
Identities = 284/284 (100%), Positives = 284/284 (100%)
Sequence:
ATGTCGTCCGCCCAACTGCTGACCGCGCAACAACTGGCCGCCCGCCTCAGCGAACCCGACCTGCTGGTCCTC
GACTGCCGTTTCGCCCTCGAAGACCCGTCCTACGGTGCCCGCGTCTATCAGGAGAACCACATTCCCGGCGCG
CATTTCGCCGACCTCGAACGGGATCTTTCCGCTCCCGTGCGCAAGGGCGTGACCGGTCGCCATCCGCTGCCG
GATCCCGCCGAGCTAGCGCTCAAGCTACAAGCCTGGGGCCTGCGCCAGGATAGCCAGGTGGTGCTCTACGAC
GACGGCCCCGGCGCATTCGCCGCCCGCGCCTGGTGGCTGTTGCATTGGCTGGGCAAGCGCGACGGGGTCTAC
CTCCTCGATGGCGGCCTGGCGGCCTGGAAGGCTGCGGGGCTGGCACTGACCAATGGCGAAAGCAGTCTTCGA
CCCGGCGATTTCCAGGGCCAGCCCGATGCCTCGCTGCTGATCGACGCCGCCACCCTCCAGGCCCAACTGGGC
CAACCCGGACTGGCGCTGCTCGATGCTCGCGCGCAGCCGCGCTTCCGTGGCGAAGTCGAGCCCATAGATCCG
GTCGCCGGGCACATTCCCGGTGCCCAGTGCGCCGCCTTCACCGACAACCTTGGCAGCGACGGCCGCTTCCTT
CCGCCGGAACAATTGCACCAGCGGTTCTCCGCCCTGCTGCGCGGTCGCCCGGTGGACGAGCTGGTTGCCTAC
TGCGGCTCCGGCGTCACCGCCTGCCACAACCTGTTCGCCCTGAGCCTCGCCGGCTTCCCGCTTCCGCGCCTC
TACGCCGGCTCGTGGAGCGAGTGGATCACCGACCCGCGCCGCCCGGTCGCCACCGGCGACTGA
Translation:
MSSAQLLTAQQLAARLSEPDLLVLDCRFALEDPSYGARVYQENHIPGAHFADLERDLSAPVRKGVTGRHPLP
DPAELALKLQAWGLRQDSQVVLYDDGPGAFAARAWWLLHWLGKRDGVYLLDGGLAAWKAAGLALTNGESSLR
PGDFQGQPDASLLIDAATLQAQLGQPGLALLDARAQPRFRGEVEPIDPVAGHIPGAQCAAFTDNLGSDGRFL
PPEQLHQRFSALLRGRPVDELVAYCGSGVTACHNLFALSLAGFPLPRLYAGSWSEWITDPRRPVATGD*
AnnotationID:2628GeneID:2620
AnnotatorUID: diggins
Modification Date/Time: 2005-05-26 07:07:23
Gene Name:sseA
Confidence Code:2
GeneProduct:3-mercaptopyruvate sulfurtransferase
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:thiosulfate sulfurtransferase
Functional Category Confidence Code:5
COGs:COG2897,cd01448,cd01449
Secondary Functional Category(ies):putative enzyme
EC Number:2.8.1.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70683.1|AE016712_1 (AE016793) thiosulfate sulfurtransferase, putative [Pseudomonas
           putida KT2440]
          Length = 284

 Score =  405 bits (1041), Expect = e-112
 Identities = 197/284 (69%), Positives = 221/284 (77%)

>gb|AAO53964.1| (AE016857) rhodanese-like domain protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 285

 Score =  403 bits (1035), Expect = e-112
 Identities = 189/283 (66%), Positives = 224/283 (79%)

>emb|CAE32393.1| (BX640442) putative sulfurtransferase [Bordetella bronchiseptica]
          Length = 283

 Score =  260 bits (664), Expect = 7e-69
 Identities = 141/281 (50%), Positives = 175/281 (62%), Gaps = 6/281 (2%)








 
Structural Features:
COG2897, SseA, Rhodanese-related sulfurtransferase 
[Inorganic ion transport and metabolism]
CD-Length = 285 residues,  97.5% aligned
Score =  284 bits (728), Expect = 7e-78
Identities = 130/285 (45%), Positives = 159/285 (55%), Gaps = 8/285 (2%)

cd01448, TST_Repeat_1, Thiosulfate sulfurtransferase (TST), N-terminal, 
inactive domain.
CD-Length = 122 residues, 100.0% aligned
Score =  138 bits (348), Expect = 9e-34

cd01449, TST_Repeat_2, Thiosulfate sulfurtransferase (TST), C-terminal, 
catalytic domain.
CD-Length = 118 residues,  98.3% aligned
Score =  132 bits (333), Expect = 6e-32







   
Genomic Context:






   
Comment:


















    ReferenceID:5243




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22388




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37496




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2620_cdd.html


    		




 
URL:

















    ReferenceID:38170




  
Author/Investigator(s):
        Ruiz-Albert J, Mundy R, Yu XJ, Beuzon CR, Holden DW.





  
Title:
        SseA is a chaperone for the SseB and SseD translocon components of the Salmonella pathogenicity-island-2-encoded type III secretion system.




  
PubMed:
        12724372


MedLine:
        




    
Source:
Microbiology. 2003 May;149(Pt 5):1103-11.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12724372&query_hl=9

















    ReferenceID:38171




  
Author/Investigator(s):
        Spallarossa A, Carpen A, Forlani F, Pagani S, Bolognesi M, Bordo D.





  
Title:
        SseA, a 3-mercaptopyruvate sulfurtransferase from Escherichia coli: crystallization and preliminary crystallographic data.




  
PubMed:
        12499560


MedLine:
        




    
Source:
Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):168-70. Epub 2002 Dec 19.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12499560&query_hl=9

















    ReferenceID:38172




  
Author/Investigator(s):
        Hama H, Kayahara T, Ogawa W, Tsuda M, Tsuchiya T.





  
Title:
        Enhancement of serine-sensitivity by a gene encoding rhodanese-like protein in Escherichia coli.




  
PubMed:
        7982894


MedLine:
        




    
Source:
J Biochem (Tokyo). 1994 Jun;115(6):1135-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7982894&query_hl=9









           
Homologs By Global Alignment 

           

       



     
Gene ID:2620
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4331
                           PA1292_tr
                           translation of PA1292
                           284
                           99.99
                           99.99
                           0
                           1503.0
     

     
     
126921
                           gb|AAG04681.1|AE004558_10
                           (AE004558) probable 3-mercaptopyruvate sulfurtransferase [Pseudomonas aeruginosa PAO1]
                           284
                           99.99
                           99.99
                           0
                           1503.0
     

     
     
126922
                           gb|AAN70683.1|AE016712_1
                           (AE016793) thiosulfate sulfurtransferase, putative [Pseudomonas putida KT2440]
                           284
                           69.36
                           77.81
                           0
                           1041.0
     

     
     
126923
                           gb|AAO53964.1|
                           (AE016857) rhodanese-like domain protein [Pseudomonas syringae pv. tomato str. DC3000]
                           285
                           66.31
                           78.59
                           1
                           1033.0
     

     
     
126928
                           gb|AAQ61905.1|
                           (AE016925) thiosulfate sulfurtransferase [Chromobacterium violaceum ATCC 12472]
                           286
                           49.30
                           61.53
                           10
                           664.0
     

     






      

          
GC ORFID: 45622How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1292Glimmer Score: 

          
Start: 723140Stop: 722286

          
Length: 855


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1292 translation of PA1292Homolog Bit Score 583.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6604 (ORF: BLASTX 395.0), t_PA1292 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGTCCGCCCAACTGCTGACCGCGCAACAACTGGCCGCCCGCCTCAGCGAACCCGACCTGCTGGTCCTC 
GACTGCCGTTTCGCCCTCGAAGACCCGTCCTACGGTGCCCGCGTCTATCAGGAGAACCACATTCCCGGCGCG 
CATTTCGCCGACCTCGAACGGGATCTTTCCGCTCCCGTGCGCAAGGGCGTGACCGGTCGCCATCCGCTGCCG 
GATCCCGCCGAGCTAGCGCTCAAGCTACAAGCCTGGGGCCTGCGCCAGGATAGCCAGGTGGTGCTCTACGAC 
GACGGCCCCGGCGCATTCGCCGCCCGCGCCTGGTGGCTGTTGCATTGGCTGGGCAAGCGCGACGGGGTCTAC 
CTCCTCGATGGCGGCCTGGCGGCCTGGAAGGCTGCGGGGCTGGCACTGACCAATGGCGAAAGCAGTCTTCGA 
CCCGGCGATTTCCAGGGCCAGCCCGATGCCTCGCTGCTGATCGACGCCGCCACCCTCCAGGCCCAACTGGGC 
CAACCCGGACTGGCGCTGCTCGATGCTCGCGCGCAGCCGCGCTTCCGTGGCGAAGTCGAGCCCATAGATCCG 
GTCGCCGGGCACATTCCCGGTGCCCAGTGCGCCGCCTTCACCGACAACCTTGGCAGCGACGGCCGCTTCCTT 
CCGCCGGAACAATTGCACCAGCGGTTCTCCGCCCTGCTGCGCGGTCGCCCGGTGGACGAGCTGGTTGCCTAC 
TGCGGCTCCGGCGTCACCGCCTGCCACAACCTGTTCGCCCTGAGCCTCGCCGGCTTCCCGCTTCCGCGCCTC 
TACGCCGGCTCGTGGAGCGAGTGGATCACCGACCCGCGCCGCCCGGTCGCCACCGGCGACTGA