ViewGene
Locus Name:
GeneID:
Location:

GeneID:261
TrivialName: PA14_45590
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45590
PAO1 Ortholog Locus:PA1458
Sequence Length:2262
Protein Length:753
Start: 4057191
Stop: 4054930
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04847.1|AE004575_6 (AE004575) probable two-component sensor [Pseudomonas aeruginosa PAO1]
Identities = 750/753 (99%), Positives = 750/753 (99%)
Sequence:
ATGAGCTTCGACGCCGATGAAGAAATCCTCCAGGACTTCCTGGTTGAGGCCGGCGAGATTCTCGAGCAATTG
TCCGAGCAACTGGTCGAGCTGGAGAGCCGGCCGGACGACATGGACCTGCTCAATGCCATTTTCCGTGGGTTC
CATACGGTCAAGGGCGGTGCCGGCTTCCTCCAGCTGAACGCGCTGGTGGAATGCTGCCACATTGCGGAAAAC
GTCTTCGATATCCTGCGCAAGGGCGAGCGGCGCGTCAGCTCCGAACTGATGGATGTGGTGCTGCAGGCGCTG
GATACGGTCAACGCGATGTTCGACCAGGTGCGCGAGCAGTCCGAACCGACCCCGGCGACGCCCGAGCTGCTG
GCGGCCCTTGCCCGCCTGGCCGAGCCTGAGGGCGCCGCGCCCGCGGAACCTGTGCAAGCGCCGCCGGCGGCC
GTTCCGCCAGCCGAGCCTGCGGCGCCGCCGGAAGCGCCCGCGCAGAGTGTCTCcAGCGATATCACCGACGAC
GAGTTCGAGCAGTTGCTGGATGCTCTCCAGGGCGACGAAGCGCCGGCCTCCGCCGTTGCAGAAGCGTCCGCG
GCTCCCGCTGGCGACGAGATCaGCGACGCCGAGTTCGAGGCGTTGCTCGACCAGTTGCACGGCAAGGGCAAG
TTCGTCCCGCCGGCGGTCTCGGCCGAGCCTGCGCAAGTCCCCGCCGAGGCGGTCGAGCCGGCCGCGGCTGCC
GCTGGTGACGATATCAGCGACGACGAGTTCGAGGCGCTGCTCGACGAACTGCACGGCAAGGGCAAGTTCGGC
GATGTCCCGGAAGCCGCCGGCACACCCGCCGCACCGGCTGCCGCGGCTCCAGCGGCTGCGCCGGCGGAGCAg
ggcAAGGCGCCGGCCGCCGCGGGCGGCGATGAGATCAGCGACGACGAGTTCGAGTCGCTGCTCGACGAACTG
CACGGCAAGGGCAAGTTCAACGGCGCCTCCGAAGCCGTGGCGGCCGCTGCGGCGGTAGCCAAGAACATCGCG
GCGAAGAGTCCCGCGGCGAAACCGGTCGCTCCGGCCAAGGCCGCCGCCGCGCGTCCGGCGGCGCCCGACAGG
CCGGCGGCGAGCGAGGCGGAGACCACCGTGCGGGTGGATACGGCGCGTCTCGACGAGATCATGAACATGGTC
GGCGAGCTGGTGCTGGTGCGTAACCGCCTGGTGCGCCTGGGCCTGAACAGCGGCGACGAGGCCATGGCCAAG
GCCGTGGCCAACCTCGACGTGGTCACCGGCGACCTGCAGATGTCGGTGATGAAGACCCGCATGCAGCCGATC
AAGAAGGTCTTCGGCCGCTTCCCGCGTCTGGTCCGCGACCTGGCGCGGAACATGAAGAAAGAGATCAACCTG
GAGCTGGTGGGCGAAGAGACCGACCTCGACAAGAACCTGGTCGAGGCCCTCGCCGATCCGCTGGTGCACCTG
GTGCGCAACGCCGTCGACCACGGCATCGAGAGTCCGGAGGAGCGCGAGGCCGCCGGCAAGCCGCGGGTCGGC
CAGGTGGTGCTGTCGGCGGAGCAGGAGGGCGACCACATCCTCCTGATGATCACCGACGACGGCAAGGGCATG
GACGCCGAGGTGCTGCGCAACAAGGCGGTGGAGAAAGGCCTGCTGGAGCGCGACGCGGCGGACCGCCTGACC
GACCTGGAGTGCTACAACCTGATCTTCGCCCCGGGTTTCTCGACCAAGACCGAGATTTCCGACGTGTCCGGC
CGCGGCGTCGGCATGGACGTGGTGAAGACCAAGATTTCCCAGCTCAACGGCACGGTCAACGTGTTCTCGCAG
AAGGGGTCCGGCTCGAAGATCGTCATCAAGGTGCCGCTGACCCTGGCGATCATGCCTACCCTTATGGTGATG
CTGGGTAGCCAGGCGTTCGCCTTCCCGCTGGTCAACGTCAACGAAATCTTCCATCTTGACCTGTCGCGCACC
AATGTCGTGGATGGCCAGGAAGTGGTGATCGTCCGCGACAAGGCCTTGCCGCTGTTCTACCTCAAGCGCTGG
CTGGTGCCGAGCGCGGCCCACGAGGAGCAGGGCGAGGGGCACGTGGTGATCCTGTCGGTCGGCACCCAGCGC
ATCGGTTTCGTCGTCGACCAGTTGGTGGGACAAGAAGAAGTGGTGATCAAGCCACTCGGCAAGATGTTGCAG
GGCACCCCCGGCATGGCGGGCGCCACCATCACCGGGGACGGGCGCATCGCCCTGATCCTCGATGTGCCGAGC
ATGCTCAAGCGTTACGCACGGCGCATCTGA
Translation:
MSFDADEEILQDFLVEAGEILEQLSEQLVELESRPDDMDLLNAIFRGFHTVKGGAGFLQLNALVECCHIAEN
VFDILRKGERRVSSELMDVVLQALDTVNAMFDQVREQSEPTPATPELLAALARLAEPEGAAPAEPVQAPPAA
VPPAEPAAPPEAPAQSVSSDITDDEFEQLLDALQGDEAPASAVAEASAAPAGDEISDAEFEALLDQLHGKGK
FVPPAVSAEPAQVPAEAVEPAAAAAGDDISDDEFEALLDELHGKGKFGDVPEAAGTPAAPAAAAPAAAPAEQ
GKAPAAAGGDEISDDEFESLLDELHGKGKFNGASEAVAAAAAVAKNIAAKSPAAKPVAPAKAAAARPAAPDR
PAASEAETTVRVDTARLDEIMNMVGELVLVRNRLVRLGLNSGDEAMAKAVANLDVVTGDLQMSVMKTRMQPI
KKVFGRFPRLVRDLARNMKKEINLELVGEETDLDKNLVEALADPLVHLVRNAVDHGIESPEEREAAGKPRVG
QVVLSAEQEGDHILLMITDDGKGMDAEVLRNKAVEKGLLERDAADRLTDLECYNLIFAPGFSTKTEISDVSG
RGVGMDVVKTKISQLNGTVNVFSQKGSGSKIVIKVPLTLAIMPTLMVMLGSQAFAFPLVNVNEIFHLDLSRT
NVVDGQEVVIVRDKALPLFYLKRWLVPSAAHEEQGEGHVVILSVGTQRIGFVVDQLVGQEEVVIKPLGKMLQ
GTPGMAGATITGDGRIALILDVPSMLKRYARRI*
AnnotationID:271GeneID:261
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 13:01:25
Gene Name:
Confidence Code:3
GeneProduct:putative two-component sensor
Cell Localization:()
Synonyms:cheA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:putative chemotaxis sensor histidine kinase CheA
Functional Category Confidence Code:5
COGs:COG0643,cd00731
Secondary Functional Category(ies):chemotaxis
EC Number:2.7.3.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55500.1| (AE016862) chemotaxis sensor histidine kinase CheA [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 748

 Score = 1080 bits (2794), Expect = 0.0
 Identities = 595/767 (77%), Positives = 647/767 (84%), Gaps = 36/767 (4%)

>gb|AAN69917.1|AE016630_8 (AE016790) chemotaxis histidine kinase CheA [Pseudomonas putida
           KT2440]
          Length = 747

 Score = 1059 bits (2739), Expect = 0.0
 Identities = 584/770 (75%), Positives = 635/770 (82%), Gaps = 43/770 (5%)

>gb|AAN56206.1|AE015758_10 (AE015758) chemotaxis protein [Shewanella oneidensis MR-1]
          Length = 758

 Score =  826 bits (2134), Expect = 0.0
 Identities = 461/778 (59%), Positives = 546/778 (70%), Gaps = 50/778 (6%)






 
Structural Features:
COG0643, CheA, Chemotaxis protein histidine kinase and related kinases 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 716 residues,  99.6% aligned
Score =  646 bits (1669), Expect = 0.0
Identities = 300/757 (39%), Positives = 422/757 (55%), Gaps = 48/757 (6%)

cd00731, CheA_reg, CheA regulatory domain
CD-Length = 132 residues, 100.0% aligned
Score =  151 bits (384), Expect = 2e-37
Identities = 62/132 (46%), Positives = 83/132 (62%), Gaps = 1/132 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:525




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17667




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37354




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:261_cdd.html


    		




 
URL:

















    ReferenceID:37957




  
Author/Investigator(s):
        Kato J, Nakamura T, Kuroda A, Ohtake H.





  
Title:
        Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa.




  
PubMed:
        10052136


MedLine:
        




    
Source:
Biosci Biotechnol Biochem. 1999 Jan;63(1):155-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10052136









           
Homologs By Global Alignment 

           

       



     
Gene ID:261
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
438
                           PA1458_tr
                           translation of PA1458
                           753
                           99.60
                           99.60
                           0
                           3734.0
     

     
     
23658
                           gb|AAG04847.1|AE004575_6
                           (AE004575) probable two-component sensor [Pseudomonas aeruginosa PAO1]
                           753
                           99.60
                           99.60
                           0
                           3734.0
     

     
     
23659
                           dbj|BAA33549.1|
                           (AB012767) CheA [Pseudomonas aeruginosa]
                           753
                           99.60
                           99.60
                           0
                           3734.0
     

     
     
23660
                           gb|AAO55500.1|
                           (AE016862) chemotaxis sensor histidine kinase CheA [Pseudomonas syringae pv. tomato str. DC3000]
                           769
                           77.50
                           84.52
                           37
                           2831.0
     

     
     
23662
                           gb|AAN69917.1|AE016630_8
                           (AE016790) chemotaxis histidine kinase CheA [Pseudomonas putida KT2440]
                           772
                           76.42
                           83.03
                           44
                           2779.0
     

     






      

          
GC ORFID: 43263How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1458Glimmer Score: 

          
Start: 896718Stop: 898979

          
Length: 2262


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1458 translation of PA1458Homolog Bit Score 1442.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7942 (ORF: BLASTX 1093.0), t_PA1458 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCTTCGACGCCGATGAAGAAATCCTCCAGGACTTCCTGGTTGAGGCCGGCGAGATTCTCGAGCAATTG 
TCCGAGCAACTGGTCGAGCTGGAGAGCCGGCCGGACGACATGGACCTGCTCAATGCCATTTTCCGTGGGTTC 
CATACGGTCAAGGGCGGTGCCGGCTTCCTCCAGCTGAACGCGCTGGTGGAATGCTGCCACATTGCGGAAAAC 
GTCTTCGATATCCTGCGCAAGGGCGAGCGGCGCGTCAGCTCCGAACTGATGGATGTGGTGCTGCAGGCGCTG 
GATACGGTCAACGCGATGTTCGACCAGGTGCGCGAGCAGTCCGAACCGACCCCGGCGACGCCCGAGCTGCTG 
GCGGCCCTTGCCCGCCTGGCCGAGCCTGAGGGCGCCGCGCCCGCGGAACCTGTGCAAGCGCCGCCGGCGGCC 
GTTCCGCCAGCCGAGCCTGCGGCGCCGCCGGAAGCGCCCGCGCAGAGTGTCTCcAGCGATATCACCGACGAC 
GAGTTCGAGCAGTTGCTGGATGCTCTCCAGGGCGACGAAGCGCCGGCCTCCGCCGTTGCAGAAGCGTCCGCG 
GCTCCCGCTGGCGACGAGATCaGCGACGCCGAGTTCGAGGCGTTGCTCGACCAGTTGCACGGCAAGGGCAAG 
TTCGTCCCGCCGGCGGTCTCGGCCGAGCCTGCGCAAGTCCCCGCCGAGGCGGTCGAGCCGGCCGCGGCTGCC 
GCTGGTGACGATATCAGCGACGACGAGTTCGAGGCGCTGCTCGACGAACTGCACGGCAAGGGCAAGTTCGGC 
GATGTCCCGGAAGCCGCCGGCACACCCGCCGCACCGGCTGCCGCGGCTCCAGCGGCTGCGCCGGCGGAGCAg 
ggcAAGGCGCCGGCCGCCGCGGGCGGCGATGAGATCAGCGACGACGAGTTCGAGTCGCTGCTCGACGAACTG 
CACGGCAAGGGCAAGTTCAACGGCGCCTCCGAAGCCGTGGCGGCCGCTGCGGCGGTAGCCAAGAACATCGCG 
GCGAAGAGTCCCGCGGCGAAACCGGTCGCTCCGGCCAAGGCCGCCGCCGCGCGTCCGGCGGCGCCCGACAGG 
CCGGCGGCGAGCGAGGCGGAGACCACCGTGCGGGTGGATACGGCGCGTCTCGACGAGATCATGAACATGGTC 
GGCGAGCTGGTGCTGGTGCGTAACCGCCTGGTGCGCCTGGGCCTGAACAGCGGCGACGAGGCCATGGCCAAG 
GCCGTGGCCAACCTCGACGTGGTCACCGGCGACCTGCAGATGTCGGTGATGAAGACCCGCATGCAGCCGATC 
AAGAAGGTCTTCGGCCGCTTCCCGCGTCTGGTCCGCGACCTGGCGCGGAACATGAAGAAAGAGATCAACCTG 
GAGCTGGTGGGCGAAGAGACCGACCTCGACAAGAACCTGGTCGAGGCCCTCGCCGATCCGCTGGTGCACCTG 
GTGCGCAACGCCGTCGACCACGGCATCGAGAGTCCGGAGGAGCGCGAGGCCGCCGGCAAGCCGCGGGTCGGC 
CAGGTGGTGCTGTCGGCGGAGCAGGAGGGCGACCACATCCTCCTGATGATCACCGACGACGGCAAGGGCATG 
GACGCCGAGGTGCTGCGCAACAAGGCGGTGGAGAAAGGCCTGCTGGAGCGCGACGCGGCGGACCGCCTGACC 
GACCTGGAGTGCTACAACCTGATCTTCGCCCCGGGTTTCTCGACCAAGACCGAGATTTCCGACGTGTCCGGC 
CGCGGCGTCGGCATGGACGTGGTGAAGACCAAGATTTCCCAGCTCAACGGCACGGTCAACGTGTTCTCGCAG 
AAGGGGTCCGGCTCGAAGATCGTCATCAAGGTGCCGCTGACCCTGGCGATCATGCCTACCCTTATGGTGATG 
CTGGGTAGCCAGGCGTTCGCCTTCCCGCTGGTCAACGTCAACGAAATCTTCCATCTTGACCTGTCGCGCACC 
AATGTCGTGGATGGCCAGGAAGTGGTGATCGTCCGCGACAAGGCCTTGCCGCTGTTCTACCTCAAGCGCTGG 
CTGGTGCCGAGCGCGGCCCACGAGGAGCAGGGCGAGGGGCACGTGGTGATCCTGTCGGTCGGCACCCAGCGC 
ATCGGTTTCGTCGTCGACCAGTTGGTGGGACAAGAAGAAGTGGTGATCAAGCCACTCGGCAAGATGTTGCAG 
GGCACCCCCGGCATGGCGGGCGCCACCATCACCGGGGACGGGCGCATCGCCCTGATCCTCGATGTGCCGAGC 
ATGCTCAAGCGTTACGCACGGCGCATCTGA