ViewGene
Locus Name:
GeneID:
Location:

GeneID:2608
TrivialName: PA14_39280
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_39280
PAO1 Ortholog Locus:PA1950
Sequence Length:927
Protein Length:308
Start: 3498751
Stop: 3497825
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05338.1|AE004621_9 (AE004621) ribokinase [Pseudomonas aeruginosa PAO1]
Identities = 303/308 (98%), Positives = 305/308 (99%)
Sequence:
ATGCAAGCGAAAGTACTGGTGGTGGGCAGCCTGAACATGGACCTGGTAGTGCGCGCGCCGCGCCTGCCACGG
GGCGGCGAGACCCTCGCCGGGCAGTCCTTCACCACCATTCCCGGCGGCAAGGGCGCCAACCAGGCGGTCGCC
GCGGCGCGTCTCGGCGCTGGGGTGGCGATGATCGGCTGCCTTGGCGACGATGCCTACGGCGACCAGTTGTAC
CGCGCGCTGCAGGCCGAGGGCATCGACTGCCAGGGCGTCGAGCGGGTGGCCGGGGAGTCCAGCGGGGTGGCG
CTGATCGTGGTCGACGACAGCAGCCAGAACGCCATCGTCATCGTCGCCGGCGGCAACGGCCACCTGTCGCCG
GCCGTGCTGGCGCGCCACGAACACTTGCTGGAGCAGGCGCAGGTGGTGGTCTGCCAGTTGGAAAGCCCGCTG
GAGACGGTTGGCCATGTGCTGCGCCGGGCCCATGCGCTGGGCAAGACGGTGATCCTCAACCCGGCGCCGGCC
ACCCGCGACGTGCCGGCGGACTGGCTGCCGCTGGTGGACTACCTGGTCCCCAACGAGACCGAAAGCGAACTG
CTCTGCCGCCTGCCGGTGGACTCCCTGGAGAGCGCCGGACGTGCCGCCGAGCGCCTGCGCGAAATGGGCGCC
GGCAGGGTGATCGTCACCCTCGGCGCCCAGGGCGCCCTGCTGGTGGGCGAGGGGCGGGTCGAGCATTTCCCG
GTGGCGCGGGTCAAGGCGCTGGATACCACGGCGGCCGGCGACACCTTCGTCGGTGGCTTCGCCGCGGCCCTT
GCGCGCGGCCTCGACGAGGCGGCGGCGATCCGCTTCGGCCAGGCCGCCGCGGCGATCTCGGTGACCCGCCTC
GGCGCGCAGACCTCGATCCCCTCGCGCGAGGAAGTCGAGCGGGCGCTCGCTGGCGAGGCTTGA
Translation:
MQAKVLVVGSLNMDLVVRAPRLPRGGETLAGQSFTTIPGGKGANQAVAAARLGAGVAMIGCLGDDAYGDQLY
RALQAEGIDCQGVERVAGESSGVALIVVDDSSQNAIVIVAGGNGHLSPAVLARHEHLLEQAQVVVCQLESPL
ETVGHVLRRAHALGKTVILNPAPATRDVPADWLPLVDYLVPNETESELLCRLPVDSLESAGRAAERLREMGA
GRVIVTLGAQGALLVGEGRVEHFPVARVKALDTTAAGDTFVGGFAAALARGLDEAAAIRFGQAAAAISVTRL
GAQTSIPSREEVERALAGEA*
AnnotationID:2616GeneID:2608
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 16:04:44
Gene Name:rbsK
Confidence Code:2
GeneProduct:ribokinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0524,pfam00294
Secondary Functional Category(ies):
EC Number:2.7.1.15
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55882.1| (AE016864) ribokinase [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 306

 Score =  379 bits (974), Expect = e-105
 Identities = 199/301 (66%), Positives = 235/301 (78%)

>gb|AAO35501.1| (AE015939) ribokinase [Clostridium tetani E88]
          Length = 308

 Score =  253 bits (647), Expect = 8e-67
 Identities = 139/298 (46%), Positives = 194/298 (65%), Gaps = 1/298 (0%)






 
Structural Features:
COG0524, RbsK, Sugar kinases, ribokinase family 
[Carbohydrate transport and metabolism]
CD-Length = 311 residues,  99.4% aligned
Score =  210 bits (534), Expect = 2e-55
Identities = 134/313 (42%), Positives = 172/313 (54%), Gaps = 14/313 (4%)

pfam00294, PfkB, pfkB family carbohydrate kinase. 
CD-Length = 298 residues, 100.0% aligned
Score =  201 bits (511), Expect = 1e-52
Identities = 120/305 (39%), Positives = 161/305 (52%), Gaps = 16/305 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5219




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22364




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32768




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2608_cdd.html


    		




 
URL:

















    ReferenceID:34357




  
Author/Investigator(s):
        Hope JN, Bell AW, Hermodson MA, Groarke JM.





  
Title:
        Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase.




  
PubMed:
        3011794


MedLine:
        




    
Source:
J Biol Chem. 1986 Jun 15;261(17):7663-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3011794

















    ReferenceID:34358




  
Author/Investigator(s):
        Iida A, Harayama S, Iino T, Hazelbauer GL.





  
Title:
        Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12.




  
PubMed:
        6327617


MedLine:
        




    
Source:
J Bacteriol. 1984 May;158(2):674-82.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6327617

















    ReferenceID:34359




  
Author/Investigator(s):
        Michiels J, Verreth C, Vanderleyden J.





  
Title:
        Sequence analysis of the Rhizobium etli ribose kinase gene rbsK and its phylogenetic position.




  
PubMed:
        1052475


MedLine:
        




    
Source:
DNA Seq. 1998;9(5-6):317-21.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10524758









           
Homologs By Global Alignment 

           

       



     
Gene ID:2608
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4317
                           PA1950_tr
                           translation of PA1950
                           308
                           98.37
                           99.02
                           0
                           1505.0
     

     
     
126757
                           gb|AAG05338.1|AE004621_9
                           (AE004621) ribokinase [Pseudomonas aeruginosa PAO1]
                           308
                           98.37
                           99.02
                           0
                           1505.0
     

     
     
126758
                           gb|AAO55882.1|
                           (AE016864) ribokinase [Pseudomonas syringae pv. tomato str. DC3000]
                           310
                           64.51
                           76.45
                           6
                           968.0
     

     
     
126759
                           gb|AAN68070.1|AE016439_5
                           (AE016783) ribokinase [Pseudomonas putida KT2440]
                           308
                           62.33
                           72.07
                           6
                           935.0
     

     
     
126760
                           emb|CAD14715.1|
                           (AL646062) PUTATIVE RIBOKINASE PROTEIN [Ralstonia solanacearum]
                           318
                           52.20
                           62.26
                           13
                           760.0
     

     






      

          
GC ORFID: 45610How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1950Glimmer Score: 

          
Start: 1455160Stop: 1456086

          
Length: 927


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1950 translation of PA1950Homolog Bit Score 584.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5857 (ORF: BLASTX 376.0), psyr_15may02_Scaffold4_revised_gene5872 (ORF: BLASTX 225.0), t_PA1950 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAAGCGAAAGTACTGGTGGTGGGCAGCCTGAACATGGACCTGGTAGTGCGCGCGCCGCGCCTGCCACGG 
GGCGGCGAGACCCTCGCCGGGCAGTCCTTCACCACCATTCCCGGCGGCAAGGGCGCCAACCAGGCGGTCGCC 
GCGGCGCGTCTCGGCGCTGGGGTGGCGATGATCGGCTGCCTTGGCGACGATGCCTACGGCGACCAGTTGTAC 
CGCGCGCTGCAGGCCGAGGGCATCGACTGCCAGGGCGTCGAGCGGGTGGCCGGGGAGTCCAGCGGGGTGGCG 
CTGATCGTGGTCGACGACAGCAGCCAGAACGCCATCGTCATCGTCGCCGGCGGCAACGGCCACCTGTCGCCG 
GCCGTGCTGGCGCGCCACGAACACTTGCTGGAGCAGGCGCAGGTGGTGGTCTGCCAGTTGGAAAGCCCGCTG 
GAGACGGTTGGCCATGTGCTGCGCCGGGCCCATGCGCTGGGCAAGACGGTGATCCTCAACCCGGCGCCGGCC 
ACCCGCGACGTGCCGGCGGACTGGCTGCCGCTGGTGGACTACCTGGTCCCCAACGAGACCGAAAGCGAACTG 
CTCTGCCGCCTGCCGGTGGACTCCCTGGAGAGCGCCGGACGTGCCGCCGAGCGCCTGCGCGAAATGGGCGCC 
GGCAGGGTGATCGTCACCCTCGGCGCCCAGGGCGCCCTGCTGGTGGGCGAGGGGCGGGTCGAGCATTTCCCG 
GTGGCGCGGGTCAAGGCGCTGGATACCACGGCGGCCGGCGACACCTTCGTCGGTGGCTTCGCCGCGGCCCTT 
GCGCGCGGCCTCGACGAGGCGGCGGCGATCCGCTTCGGCCAGGCCGCCGCGGCGATCTCGGTGACCCGCCTC 
GGCGCGCAGACCTCGATCCCCTCGCGCGAGGAAGTCGAGCGGGCGCTCGCTGGCGAGGCTTGA