ViewGene
Locus Name:
GeneID:
Location:

GeneID:2602
TrivialName: PA14_68200
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_68200
PAO1 Ortholog Locus:PA5163
Sequence Length:882
Protein Length:293
Start: 6084412
Stop: 6085293
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08548.1|AE004929_3 (AE004929) glucose-1-phosphate thymidylyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 292/293 (99%), Positives = 293/293 (100%)
Sequence:
ATGAAACGCAAGGGCATCATCCTCGCCGGAGGCTCGGGCACCCGCCTGCACCCGGCAACGCTGGCCATCTCC
AAGCAGCTGCTGCCGGTGTACGACAAGCCGATGATCTACTACCCGCTCAGTACCCTGATGCTGGCGGGCATC
CGCGAGATACTGATCATCTCGACCCCACAGGACACCCCACGCTTCCAGCAGTTGCTGGGCGACGGTTCGAAC
TGGGGCCTGGACCTGCAATATGCCGTGCAACCGTCGCCGGACGGCCTGGCCCAGGCCTTCCTGATCGGCGAG
TCGTTCATCGGCAACGACCTCAGCGCGCTGGTCCTGGGCGACAACCTCTATTACGGCCACGACTTCCACGAG
TTGCTCGGCAGCGCTTCGCAGCGCCAGACCGGCGCCAGCGTCTTCGCCTACCACGTGCTGGACCCGGAGCGC
TACGGCGTGGTCGAGTTCGACCAGGGCGGCAAGGCCATCAGCCTGGAAGAGAAGCCACTGAAGCCGAAGTCG
AACTACGCGGTCACCGGCCTGTATTTCTACGACCAGCAGGTGGTGGACATCGCGAGGGACCTGAAGCCTTCG
CCGCGCGGCGAGCTGGAGATCACCGACGTCAACCGCGCCTACCTGGAGCGCGGCCAGCTCAGCGTGGAGATC
ATGGGCCGCGGCTACGCCTGGCTGGATACCGGCACCCACGATTCGCTGCTCGAGGCCGGCCAGTTCATCGCC
ACCCTGGAGAACCGCCAGGGACTCAAGGTGGCCTGCCCGGAAGAGATCGCCTACCGGCAGAAGTGGATCGAC
GCCGCGCAACTGGAAAAACTCGCCGCGCCGCTGGCCAAGAACGGCTACGGCCAATACCTCAAGCGCCTGCTG
ACCGAGACCGTGTACTGA
Translation:
MKRKGIILAGGSGTRLHPATLAISKQLLPVYDKPMIYYPLSTLMLAGIREILIISTPQDTPRFQQLLGDGSN
WGLDLQYAVQPSPDGLAQAFLIGESFIGNDLSALVLGDNLYYGHDFHELLGSASQRQTGASVFAYHVLDPER
YGVVEFDQGGKAISLEEKPLKPKSNYAVTGLYFYDQQVVDIARDLKPSPRGELEITDVNRAYLERGQLSVEI
MGRGYAWLDTGTHDSLLEAGQFIATLENRQGLKVACPEEIAYRQKWIDAAQLEKLAAPLAKNGYGQYLKRLL
TETVY*
AnnotationID:2610GeneID:2602
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 13:01:00
Gene Name:rmlA
Confidence Code:1
GeneProduct:glucose-1-phosphate thymidylyltransferase
Cell Localization:()
Synonyms:rfbA
Cell Localization Confidence Code:5
MolecularFunction:dTDP-glucose pyrophosphorylase
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1209,pfam00483
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:2.7.7.24
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD84588.1| (BX321858) ADP-glucose pyrophosphorylase [Nitrosomonas europaea
           ATCC 19718]
          Length = 293

 Score =  477 bits (1227), Expect = e-134
 Identities = 236/293 (80%), Positives = 259/293 (88%)

>gb|AAN67403.1|AE016367_3 (AE016780) glucose-1-phosphate thymidylyltransferase [Pseudomonas
           putida KT2440]
          Length = 293

 Score =  474 bits (1221), Expect = e-133
 Identities = 230/293 (78%), Positives = 258/293 (88%)

>emb|CAA43074.1| (X60665) glucose-1-phosphate thymidylyltransferase [Salmonella
           enterica]
          Length = 292

 Score =  460 bits (1184), Expect = e-129
 Identities = 219/286 (76%), Positives = 256/286 (89%)








 
Structural Features:
COG1209, RfbA, dTDP-glucose pyrophosphorylase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 286 residues, 100.0% aligned
Score =  427 bits (1099), Expect = 9e-121
Identities = 173/289 (59%), Positives = 208/289 (71%), Gaps = 3/289 (1%)

pfam00483, NTP_transferase, Nucleotidyl transferase.
CD-Length = 246 residues,  99.6% aligned
Score =  268 bits (687), Expect = 5e-73
Identities = 111/245 (45%), Positives = 140/245 (57%), Gaps = 7/245 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5207




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22352




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34910




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2602_cdd.html


    		




 
URL:

















    ReferenceID:35772




  
Author/Investigator(s):
        Rajakumar K, Jost BH, Sasakawa C, Okada N, Yoshikawa M, Adler B.





  
Title:
        Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence.




  
PubMed:
        8157605


MedLine:
        




    
Source:
J Bacteriol. 1994 Apr;176(8):2362-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8157605

















    ReferenceID:35775




  
Author/Investigator(s):
        Dasgupta T, Lam JS.





  
Title:
        Identification of rfbA, involved in B-band lipopolysaccharide biosynthesis in Pseudomonas aeruginosa serotype O5.




  
PubMed:
        7537247


MedLine:
        




    
Source:
Infect Immun. 1995 May;63(5):1674-80.

Comment in:
    * Infect Immun. 1995 Dec;63(12):4964-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7537247









           
Homologs By Global Alignment 

           

       



     
Gene ID:2602
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4311
                           PA5163_tr
                           translation of PA5163
                           293
                           99.65
                           99.99
                           0
                           1510.0
     

     
     
126479
                           gb|AAG08548.1|AE004929_3
                           (AE004929) glucose-1-phosphate thymidylyltransferase [Pseudomonas aeruginosa PAO1]
                           293
                           99.65
                           99.99
                           0
                           1510.0
     

     
     
126480
                           emb|CAC82197.1|
                           (AJ298592) glucose-1-phosphate thymidyltransferase [Pseudomonas aeruginosa]
                           293
                           99.65
                           99.99
                           0
                           1510.0
     

     
     
126481
                           emb|CAD84588.1|
                           (BX321858) ADP-glucose pyrophosphorylase [Nitrosomonas europaea ATCC 19718]
                           293
                           80.54
                           88.39
                           0
                           1227.0
     

     
     
126482
                           gb|AAN67403.1|AE016367_3
                           (AE016780) glucose-1-phosphate thymidylyltransferase [Pseudomonas putida KT2440]
                           293
                           78.49
                           88.05
                           0
                           1221.0
     

     






      

          
GC ORFID: 45604How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5163Glimmer Score: 

          
Start: 5348098Stop: 5348979

          
Length: 882


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5163 translation of PA5163Homolog Bit Score 586.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene5109 (ORF: BLASTX 484.0), t_PA5163 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACGCAAGGGCATCATCCTCGCCGGAGGCTCGGGCACCCGCCTGCACCCGGCAACGCTGGCCATCTCC 
AAGCAGCTGCTGCCGGTGTACGACAAGCCGATGATCTACTACCCGCTCAGTACCCTGATGCTGGCGGGCATC 
CGCGAGATACTGATCATCTCGACCCCACAGGACACCCCACGCTTCCAGCAGTTGCTGGGCGACGGTTCGAAC 
TGGGGCCTGGACCTGCAATATGCCGTGCAACCGTCGCCGGACGGCCTGGCCCAGGCCTTCCTGATCGGCGAG 
TCGTTCATCGGCAACGACCTCAGCGCGCTGGTCCTGGGCGACAACCTCTATTACGGCCACGACTTCCACGAG 
TTGCTCGGCAGCGCTTCGCAGCGCCAGACCGGCGCCAGCGTCTTCGCCTACCACGTGCTGGACCCGGAGCGC 
TACGGCGTGGTCGAGTTCGACCAGGGCGGCAAGGCCATCAGCCTGGAAGAGAAGCCACTGAAGCCGAAGTCG 
AACTACGCGGTCACCGGCCTGTATTTCTACGACCAGCAGGTGGTGGACATCGCGAGGGACCTGAAGCCTTCG 
CCGCGCGGCGAGCTGGAGATCACCGACGTCAACCGCGCCTACCTGGAGCGCGGCCAGCTCAGCGTGGAGATC 
ATGGGCCGCGGCTACGCCTGGCTGGATACCGGCACCCACGATTCGCTGCTCGAGGCCGGCCAGTTCATCGCC 
ACCCTGGAGAACCGCCAGGGACTCAAGGTGGCCTGCCCGGAAGAGATCGCCTACCGGCAGAAGTGGATCGAC 
GCCGCGCAACTGGAAAAACTCGCCGCGCCGCTGGCCAAGAACGGCTACGGCCAATACCTCAAGCGCCTGCTG 
ACCGAGACCGTGTACTGA