ViewGene
Locus Name:
GeneID:
Location:

GeneID:2595
TrivialName: PA14_71530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71530
PAO1 Ortholog Locus:PA5420
Sequence Length:858
Protein Length:285
Start: 6373744
Stop: 6374601
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08805.1|AE004954_7 (AE004954) formyltetrahydrofolate deformylase [Pseudomonas aeruginosa PAO1]
Identities = 285/285 (100%), Positives = 285/285 (100%)
Sequence:
ATGAGCCGTACCCCCGACACCTGGATCCTCACCGCCGACAGTCCCAGCCTGCTGGGCACCGTCGACGTGGTG
ACGCGCTACCTCTTCGAGCAGCGCTGCTACGTCACCGAGCACCATTCGTTCGACGACCGCCTGGCGCAACGC
TTCTTCATCCGCGTGGAGTTCCGCGCCGGCGACGGCTTCGACGAAGCCTCCTTCCGCGCCGGACTGGCCGAA
CGGGTGGCACCGTTCGGCATGCGTACCGAGCTGACCGCGCCGGGCTACCGCAGCCAGGTGGCGATCATGGTT
TCCAAGGCCGACCACTGCCTGAACGACCTGCTCTACCGCCAGCGCATCGGCCAGTTGCCGATGGACGTGGTG
GCGGTGATTTCCAATCACCCGGACCTGGAGCCGCTGGCGCGCTGGCACGGCATTCCCTACCACCACTTCCCG
CTCGACCCGAACGACAAGCCGGCCCAGGAAGCCAGGGTCTGGCAGGTGCTCGAAGAGAGTGGCGCCGAGCTG
GTGATCCTCGCTCGCTACATGCAGGTGCTGTCGCCGGAACTGTGCCGACGCCTGGACGGCTGGGCGATCAAC
ATCCATCACTCCCTGCTGCCCGGCTTCAAGGGCGCCAAGCCGTACCACCAGGCCTACCAGAAGGGCGTGAAG
CTGGTCGGCGCTACCGCCCACTACATCAACAACGACCTCGACGAAGGGCCGATCATCGCCCAGGGCGTCGAG
ACGGTCGACCACGCCCACTACCCCGAGGACCTGATCGCCAAGGGCCGCGACATCGAGTGCCTGACCCTGGCG
CGGGCGGTGGGCTACCACATCGAGCGGAGGGTATTCCTCAACGCCAATCGGACCGTGGTGCTGTAA
Translation:
MSRTPDTWILTADSPSLLGTVDVVTRYLFEQRCYVTEHHSFDDRLAQRFFIRVEFRAGDGFDEASFRAGLAE
RVAPFGMRTELTAPGYRSQVAIMVSKADHCLNDLLYRQRIGQLPMDVVAVISNHPDLEPLARWHGIPYHHFP
LDPNDKPAQEARVWQVLEESGAELVILARYMQVLSPELCRRLDGWAINIHHSLLPGFKGAKPYHQAYQKGVK
LVGATAHYINNDLDEGPIIAQGVETVDHAHYPEDLIAKGRDIECLTLARAVGYHIERRVFLNANRTVVL*
AnnotationID:2603GeneID:2595
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 14:02:16
Gene Name:purU2
Confidence Code:2
GeneProduct:formyltetrahydrofolate deformylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0788
Secondary Functional Category(ies):
EC Number:3.5.1.10
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65958.1|AE016224_2 (AE016775) formyltetrahydrofolate deformylase [Pseudomonas putida
           KT2440]
          Length = 285

 Score =  511 bits (1316), Expect = e-144
 Identities = 248/285 (87%), Positives = 259/285 (90%)

>gb|AAO54000.1| (AE016857) formyltetrahydrofolate deformylase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 285

 Score =  509 bits (1312), Expect = e-144
 Identities = 248/285 (87%), Positives = 260/285 (91%)

>emb|CAD17216.1| (AL646076) PROBABLE FORMYLTETRAHYDROFOLATE DEFORMYLASE PROTEIN
           [Ralstonia solanacearum]
          Length = 290

 Score =  310 bits (793), Expect = 8e-84
 Identities = 162/285 (56%), Positives = 196/285 (68%), Gaps = 3/285 (1%)








 
Structural Features:
COG0788, PurU, Formyltetrahydrofolate hydrolase [Nucleotide transport and metabolism]
CD-Length = 287 residues,  99.7% aligned
Score =  423 bits (1090), Expect = 9e-120
Identities = 155/286 (54%), Positives = 189/286 (66%), Gaps = 1/286 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5193




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22338




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34688




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2595_cdd.html


    		




 
URL:

















    ReferenceID:35642




  
Author/Investigator(s):
        Nagy PL, McCorkle GM, Zalkin H.





  
Title:
        purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis.




  
PubMed:
        8226647


MedLine:
        




    
Source:
J Bacteriol. 1993 Nov;175(21):7066-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8226647









           
Homologs By Global Alignment 

           

       



     
Gene ID:2595
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4303
                           PA5420_tr
                           translation of PA5420
                           285
                           99.99
                           99.99
                           0
                           1512.0
     

     
     
126148
                           gb|AAG08805.1|AE004954_7
                           (AE004954) formyltetrahydrofolate deformylase [Pseudomonas aeruginosa PAO1]
                           285
                           99.99
                           99.99
                           0
                           1512.0
     

     
     
126149
                           gb|AAN65958.1|AE016224_2
                           (AE016775) formyltetrahydrofolate deformylase [Pseudomonas putida KT2440]
                           285
                           87.01
                           90.87
                           0
                           1316.0
     

     
     
126150
                           gb|AAO54000.1|
                           (AE016857) formyltetrahydrofolate deformylase [Pseudomonas syringae pv. tomato str. DC3000]
                           285
                           87.01
                           91.22
                           0
                           1312.0
     

     
     
126151
                           emb|CAD17216.1|
                           (AL646076) PROBABLE FORMYLTETRAHYDROFOLATE DEFORMYLASE PROTEIN [Ralstonia solanacearum]
                           293
                           55.97
                           67.91
                           11
                           798.0
     

     






      

          
GC ORFID: 45597How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5420Glimmer Score: 

          
Start: 5637433Stop: 5638290

          
Length: 858


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5420 translation of PA5420Homolog Bit Score 587.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene261 (ORF: BLASTX 289.0), t_PA4314 (ORF: BLASTX 243.0), psyr_15may02_Scaffold5_revised_gene6186 (ORF: BLASTX 232.0), t_PA5420 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCGTACCCCCGACACCTGGATCCTCACCGCCGACAGTCCCAGCCTGCTGGGCACCGTCGACGTGGTG 
ACGCGCTACCTCTTCGAGCAGCGCTGCTACGTCACCGAGCACCATTCGTTCGACGACCGCCTGGCGCAACGC 
TTCTTCATCCGCGTGGAGTTCCGCGCCGGCGACGGCTTCGACGAAGCCTCCTTCCGCGCCGGACTGGCCGAA 
CGGGTGGCACCGTTCGGCATGCGTACCGAGCTGACCGCGCCGGGCTACCGCAGCCAGGTGGCGATCATGGTT 
TCCAAGGCCGACCACTGCCTGAACGACCTGCTCTACCGCCAGCGCATCGGCCAGTTGCCGATGGACGTGGTG 
GCGGTGATTTCCAATCACCCGGACCTGGAGCCGCTGGCGCGCTGGCACGGCATTCCCTACCACCACTTCCCG 
CTCGACCCGAACGACAAGCCGGCCCAGGAAGCCAGGGTCTGGCAGGTGCTCGAAGAGAGTGGCGCCGAGCTG 
GTGATCCTCGCTCGCTACATGCAGGTGCTGTCGCCGGAACTGTGCCGACGCCTGGACGGCTGGGCGATCAAC 
ATCCATCACTCCCTGCTGCCCGGCTTCAAGGGCGCCAAGCCGTACCACCAGGCCTACCAGAAGGGCGTGAAG 
CTGGTCGGCGCTACCGCCCACTACATCAACAACGACCTCGACGAAGGGCCGATCATCGCCCAGGGCGTCGAG 
ACGGTCGACCACGCCCACTACCCCGAGGACCTGATCGCCAAGGGCCGCGACATCGAGTGCCTGACCCTGGCG 
CGGGCGGTGGGCTACCACATCGAGCGGAGGGTATTCCTCAACGCCAATCGGACCGTGGTGCTGTAA