ViewGene
Locus Name:
GeneID:
Location:

GeneID:2584
TrivialName: PA14_50530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_50530
PAO1 Ortholog Locus:PA1073
Sequence Length:924
Protein Length:307
Start: 4488332
Stop: 4489255
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04462.1|AE004539_4 (AE004539) branched-chain amino acid transport protein BraD [Pseudomonas aeruginosa PAO1]
Identities = 307/307 (100%), Positives = 307/307 (100%)
Sequence:
ATGCCCGAGATTTATCATTACCTACAACAATTGGTTAACGGCCTGACCGTCGGCAGCACCTATGCGCTGATC
GCGATCGGCTACACCATGGTCTACGGCATCATCGGCATGATCAACTTCGCCCACGGCGAGGTGTACATGATC
GGCTCGTACATCGCCTTCATCGCCATCACCCTGCTGGCGATGATGGGGCTGGACAGCGTTCCGCTGATGATG
CTCGCCGCGTTCGCCGCCAGCATCATCGTCACCAGTGCCTTCGGCTACAGCATCGAGCGGGTCGCCTACCGC
CCGTTGCGCGGCGGCAACCGCCTGATCCCGCTGATCTCCGCGATCGGCATGTCGATCTTCCTGCAGAACGCC
GTGATGCTCTCGCAGGACTCCAAGGAAAAGGCCATCCCGACCCTGCTGCCCGGCAACTTCGTGTTCGGCGAA
AGCAGCATGAACGGCGTGGTGATCTCCTATATGCAGATCCTGATCTTCGTCGTCACCTTCCTGGTGATGTTC
GGCCTCACCCTGTTCATCTCCCGCTCCCGCCTGGGCCGCGCCTGCCGCGCCTGCGCCGAGGACCTGAAGATG
ACCAACCTGCTGGGTATCAACAGCAACAACATCATCGCCCTCACCTTCGTCATCGGCGCCGCCCTGGCCGCC
GTCGCCGCGGTGCTGCTGGGCATGCAGTACGGCGTGATCAACCCCGGAATCGGCTTCCTCGCCGGGATCAAG
GCGTTCACCGCCGCGGTGCTCGGCGGCATCGGCAGCATCCCCGGCGCCATGCTCGGCGGCCTGCTGCTGGGC
GTCGCCGAAGCCTTCGGCGCCGACGTGTTCGGCGACCAGTACAAGGACGTGGTGGCCTTCGGCCTGCTGATC
CTGGTGCTGCTGTTCCGACCTACCGGCATCCTCGGTCGTCCGGAGGTGGAAAAAGTATGA
Translation:
MPEIYHYLQQLVNGLTVGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYIAFIAITLLAMMGLDSVPLMM
LAAFAASIIVTSAFGYSIERVAYRPLRGGNRLIPLISAIGMSIFLQNAVMLSQDSKEKAIPTLLPGNFVFGE
SSMNGVVISYMQILIFVVTFLVMFGLTLFISRSRLGRACRACAEDLKMTNLLGINSNNIIALTFVIGAALAA
VAAVLLGMQYGVINPGIGFLAGIKAFTAAVLGGIGSIPGAMLGGLLLGVAEAFGADVFGDQYKDVVAFGLLI
LVLLFRPTGILGRPEVEKV*
AnnotationID:2592GeneID:2584
AnnotatorUID: danlee
Modification Date/Time: 2004-05-19 17:05:29
Gene Name:braD
Confidence Code:1
GeneProduct:branched-chain amino acid transport protein BraD
Cell Localization:(4) Inner membrane protein
Synonyms:PA1073
Cell Localization Confidence Code:1
MolecularFunction:
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0559
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
COG0559, LivH, Branched-chain amino acid ABC-type transport system, permease 
components [Amino acid transport and metabolism]
      CD-Length = 297 residues, 100.0% aligned
      Score =  223 bits (570), Expect = 2e-59






   
Genomic Context:
Adjacent to genes BraC, BraE, BraF, and BraG, reported to constitute a
transport system for branched-chain amino acids 






   
Comment:


















    ReferenceID:5171




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22316




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32221




  
Author/Investigator(s):
        Hoshino T, Kose K.





  
Title:
        Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.




  
PubMed:
        91008919


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5531-9






    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2584
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4289
                           PA1073_tr
                           translation of PA1073
                           307
                           99.99
                           99.99
                           0
                           1514.0
     

     
     
125936
                           gb|AAG04462.1|AE004539_4
                           (AE004539) branched-chain amino acid transport protein BraD [Pseudomonas aeruginosa PAO1]
                           307
                           99.99
                           99.99
                           0
                           1514.0
     

     
     
125937
                           dbj|BAA14255.1|
                           (D90223) integral membrane protein [Pseudomonas aeruginosa]
                           307
                           99.99
                           99.99
                           0
                           1514.0
     

     
     
125938
                           gb|AAR19311.1|
                           (AY426537) putative branched-chain amino acid transporter permease protein [symbiont bacterium of Paederus fuscipes]
                           307
                           94.46
                           97.39
                           0
                           1451.0
     

     
     
125939
                           gb|AAN66765.1|AE016304_10
                           (AE016778) branched-chain amino acid ABC transporter, permease protein [Pseudomonas putida KT2440]
                           307
                           83.06
                           90.87
                           0
                           1299.0
     

     






      

          
GC ORFID: 45586How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1073Glimmer Score: 

          
Start: 465576Stop: 464653

          
Length: 924


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1073 translation of PA1073Homolog Bit Score 587.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6074 (ORF: BLASTX 497.0), psyr_15may02_Scaffold8_revised_gene7643 (ORF: BLASTX 333.0), t_PA4912 (ORF: BLASTX 331.0), t_PA1073 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCGAGATTTATCATTACCTACAACAATTGGTTAACGGCCTGACCGTCGGCAGCACCTATGCGCTGATC 
GCGATCGGCTACACCATGGTCTACGGCATCATCGGCATGATCAACTTCGCCCACGGCGAGGTGTACATGATC 
GGCTCGTACATCGCCTTCATCGCCATCACCCTGCTGGCGATGATGGGGCTGGACAGCGTTCCGCTGATGATG 
CTCGCCGCGTTCGCCGCCAGCATCATCGTCACCAGTGCCTTCGGCTACAGCATCGAGCGGGTCGCCTACCGC 
CCGTTGCGCGGCGGCAACCGCCTGATCCCGCTGATCTCCGCGATCGGCATGTCGATCTTCCTGCAGAACGCC 
GTGATGCTCTCGCAGGACTCCAAGGAAAAGGCCATCCCGACCCTGCTGCCCGGCAACTTCGTGTTCGGCGAA 
AGCAGCATGAACGGCGTGGTGATCTCCTATATGCAGATCCTGATCTTCGTCGTCACCTTCCTGGTGATGTTC 
GGCCTCACCCTGTTCATCTCCCGCTCCCGCCTGGGCCGCGCCTGCCGCGCCTGCGCCGAGGACCTGAAGATG 
ACCAACCTGCTGGGTATCAACAGCAACAACATCATCGCCCTCACCTTCGTCATCGGCGCCGCCCTGGCCGCC 
GTCGCCGCGGTGCTGCTGGGCATGCAGTACGGCGTGATCAACCCCGGAATCGGCTTCCTCGCCGGGATCAAG 
GCGTTCACCGCCGCGGTGCTCGGCGGCATCGGCAGCATCCCCGGCGCCATGCTCGGCGGCCTGCTGCTGGGC 
GTCGCCGAAGCCTTCGGCGCCGACGTGTTCGGCGACCAGTACAAGGACGTGGTGGCCTTCGGCCTGCTGATC 
CTGGTGCTGCTGTTCCGACCTACCGGCATCCTCGGTCGTCCGGAGGTGGAAAAAGTATGA