ViewGene
Locus Name:
GeneID:
Location:

GeneID:2579
TrivialName: PA14_58770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58770
PAO1 Ortholog Locus:PA4528
Sequence Length:873
Protein Length:290
Start: 5237206
Stop: 5238078
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAA25734.1| (M32066) pilin biogenesis protein (pilD) [Pseudomonas aeruginosa]
Identities = 288/290 (99%), Positives = 288/290 (99%)
Sequence:
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTG
GTCGGCAGCTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCC
CGCGAAGCGCTCGGGCTGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGC
CCGCGCTGCGGCCACGAGATCCGCCCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAA
TGCTCGTCCTGCAAGGCCGCCATCGGCAAACGCTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCTGGC
TACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCGCGATGCTCCTGCTGACCTGGGGTCTGCTGGCG
ATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTGCTGCCGCTGCTCTGGCTGGGCCTG
ATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGGCGATGCGCTGTTCGGCGCGGTGTTCGGCTACCTGAGC
CTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTG
CTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTAT
CTGGCGATTGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGA
TTCAAATGA
Translation:
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSAC
PRCGHEIRPWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLA
MSLIDADHQLLPDVLVLPLLWLGLIANHFGLFASLGDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKL
LAMLGAWGGWQILPLTILLSSLVGAILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAG
FK*
AnnotationID:2587GeneID:2579
AnnotatorUID: diggins
Modification Date/Time: 2005-05-04 16:04:20
Gene Name:pilD
Confidence Code:1
GeneProduct: type 4 prepilin peptidase PilD
Cell Localization:()
Synonyms:xcpA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name: type 4 fimbrial biogenesis protein
Functional Category Confidence Code:5
COGs:COG1989
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54458.1| (AE016859) type IV pilus prepilin peptidase PilD [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 290

 Score =  482 bits (1240), Expect = e-135
 Identities = 229/290 (78%), Positives = 254/290 (87%)


>gb|AAG18589.1|AF297457_4 (AF297457) pilin biogenesis protein PilD [Pseudomonas fluorescens]
          Length = 290

 Score =  414 bits (1063), Expect = e-115
 Identities = 199/290 (68%), Positives = 230/290 (79%), Gaps = 1/290 (0%)







 
Structural Features:
COG1989, PulO, Type II secretory pathway, prepilin signal peptidase PulO 
and related peptidases 
[Cell motility and secretion /
 Posttranslational modification, protein turnover, chaperones / 
Intracellular trafficking and secretion]








   
Genomic Context:






   
Comment:


















    ReferenceID:5161




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22306




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31573




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2579_cdd.html


    		




 
URL:

















    ReferenceID:32976




  
Author/Investigator(s):
        Bally M, Filloux A, Akrim M, Ball G, Lazdunski A, Tommassen J.





  
Title:
        Protein secretion in Pseudomonas aeruginosa: characterization of seven xcp genes and processing of secretory apparatus components by prepilin peptidase.




  
PubMed:
        1588814


MedLine:
        




    
Source:
Mol Microbiol. 1992 May;6(9):1121-31.
Erratum in:
Mol Microbiol 1992 Sep;6(18):2745.








    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1588814

















    ReferenceID:32980




  
Author/Investigator(s):
        Strom MS, Nunn DN, Lory S.





  
Title:
        Posttranslational processing of type IV prepilin and homologs by PilD of Pseudomonas aeruginosa.




  
PubMed:
        8057924


MedLine:
        




    
Source:
Methods Enzymol. 1994;235:527-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8057924

















    ReferenceID:32981




  
Author/Investigator(s):
        Strom MS, Bergman P, Lory S.





  
Title:
         
Identification of active-site cysteines in the conserved domain of PilD, the bifunctional type IV pilin leader peptidase/N-methyltransferase of Pseudomonas aeruginosa.




  
PubMed:
        8340405


MedLine:
        




    
Source:
J Biol Chem. 1993 Jul 25;268(21):15788-94.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8340405

















    ReferenceID:32982




  
Author/Investigator(s):
        Strom MS, Nunn DN, Lory S.





  
Title:
        A single bifunctional enzyme, PilD, catalyzes cleavage and N-methylation of proteins belonging to the type IV pilin family.




  
PubMed:
        8096341


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2404-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8096341

















    ReferenceID:32983




  
Author/Investigator(s):
        Strom MS, Lory S.





  
Title:
        Structure-function and biogenesis of the type IV pili.




  
PubMed:
        7903032


MedLine:
        




    
Source:
Structure-function and biogenesis of the type IV pili.







    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7903032

















    ReferenceID:32984




  
Author/Investigator(s):
        Strom MS, Lory S.





  
Title:
        Kinetics and sequence specificity of processing of prepilin by PilD, the type IV leader peptidase of Pseudomonas aeruginosa.




  
PubMed:
        1429457


MedLine:
        




    
Source:
J Bacteriol. 1992 Nov;174(22):7345-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1429457

















    ReferenceID:32985




  
Author/Investigator(s):
        Nunn DN, Lory S.





  
Title:
        Components of the protein-excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidase.




  
PubMed:
        1309616


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):47-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1309616

















    ReferenceID:32986




  
Author/Investigator(s):
        Nunn DN, Lory S.





  
Title:
        Product of the Pseudomonas aeruginosa gene pilD is a prepilin leader peptidase.




  
PubMed:
        1901657


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3281-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1901657

















    ReferenceID:32987




  
Author/Investigator(s):
        Strom MS, Nunn D, Lory S.





  
Title:
        Multiple roles of the pilus biogenesis protein pilD: involvement of pilD in excretion of enzymes from Pseudomonas aeruginosa.




  
PubMed:
        1671384


MedLine:
        




    
Source:
J Bacteriol. 1991 Feb;173(3):1175-80.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1671384









           
Homologs By Global Alignment 

           

       



     
Gene ID:2579
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
125725
                           gb|AAA25734.1|
                           (M32066) pilin biogenesis protein (pilD) [Pseudomonas aeruginosa]
                           290
                           99.31
                           99.31
                           0
                           1521.0
     

     
     
4283
                           PA4528_tr
                           translation of PA4528
                           290
                           98.96
                           98.96
                           0
                           1517.0
     

     
     
125726
                           gb|AAG07916.1|AE004867_2
                           (AE004867) type 4 prepilin peptidase PilD [Pseudomonas aeruginosa PAO1]
                           290
                           98.96
                           98.96
                           0
                           1517.0
     

     
     
125727
                           gb|AAA26023.1|
                           (M61096) xcpA [Pseudomonas aeruginosa]
                           290
                           98.96
                           98.96
                           0
                           1517.0
     

     
     
125729
                           gb|AAO54458.1|
                           (AE016859) type IV pilus prepilin peptidase PilD [Pseudomonas syringae pv. tomato str. DC3000]
                           290
                           78.96
                           87.58
                           0
                           1240.0
     

     






      

          
GC ORFID: 45581How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4528Glimmer Score: 

          
Start: 4500885Stop: 4501757

          
Length: 873


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4528 translation of PA4528Homolog Bit Score 588.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2537 (ORF: BLASTX 473.0), t_PA4528 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTG 
GTCGGCAGCTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCC 
CGCGAAGCGCTCGGGCTGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGC 
CCGCGCTGCGGCCACGAGATCCGCCCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAA 
TGCTCGTCCTGCAAGGCCGCCATCGGCAAACGCTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCTGGC 
TACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCGCGATGCTCCTGCTGACCTGGGGTCTGCTGGCG 
ATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTGCTGCCGCTGCTCTGGCTGGGCCTG 
ATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGGCGATGCGCTGTTCGGCGCGGTGTTCGGCTACCTGAGC 
CTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTG 
CTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG 
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTAT 
CTGGCGATTGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGA 
TTCAAATGA