ViewGene
Locus Name:
GeneID:
Location:

GeneID:2578
TrivialName: PA14_19100
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19100
PAO1 Ortholog Locus:PA3479
Sequence Length:888
Protein Length:295
Start: 1648632
Stop: 1649519
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAA62128.1| (L28170) rhamnosyl transferase [Pseudomonas aeruginosa]
Identities = 295/295 (100%), Positives = 295/295 (100%)
Sequence:
ATGCGGCGCGAAAGTCTGTTGGTATCGGTTTGCAAGGGCCTGCGGGTACATGTCGAGCGCGTTGGGCAGGAT
CCCGGGCGCAGCACGGTGATGCTGGTCAACGGCGCGATGGCGACCACCGCCTCGTTCGCCCGGACCTGCAAG
TGCCTGGCCGAACATTTCAACGTGGTGCTGTTCGACCTGCCCTTCGCCGGGCAGTCGCGGCAGCACAACCCG
CAGCGCGGGTTGATCACCAAGGACGACGAGGTGGAAATCCTCCTGGCGCTGATCGAGCGCTTCGAGGTCAAT
CACCTGGTCTCCGCGTCCTGGGGCGGTATCTCCACGCTGCTGGCGCTGTCGCGCAATCCGCGCGGTATCCGC
AGCTCGGTGGTGATGGCATTCGCCCCTGGACTGAACCAGGCGATGCTCGACTACGTCGGGCGGGCGCAGGCG
CTGATCGAGCTGGACGACAAGTCGGCGATCGGCCATCTGCTCAACGAGACCGTCGGCAAATACCTGCCGCCG
CGCCTGAAAGCCAGCAACCATCAGCACATGGCTTCGCTGGCCACCGGCGAATACGAGCAGGCGCGCTTTCAC
ATCGACCAGGTGCTGGCGCTCAACGATCGGGGCTACCTGGCTTGCCTGGAGCGGATCCAGAGCCACGTGCAT
TTCATCAACGGCAGCTGGGACGAGTACACCACCGCCGAGGACGCCCGCCAGTTCCGCGACTACCTGCCGCAC
TGCAGTTTCTCGCGGGTGGAGGGCACCGGGCATTTCCTCGACCTGGAGTCCAAGCTGGCCGCGGTACGCGTG
CACCGCGCCCTGCTCGAGCACCTGCTGAAGCAACCGGAGCCGCAGCGGGCGGAACGCGCGGCGGGATTCCAC
GAGATGGCCATCGGCTACGCCTGA
Translation:
MRRESLLVSVCKGLRVHVERVGQDPGRSTVMLVNGAMATTASFARTCKCLAEHFNVVLFDLPFAGQSRQHNP
QRGLITKDDEVEILLALIERFEVNHLVSASWGGISTLLALSRNPRGIRSSVVMAFAPGLNQAMLDYVGRAQA
LIELDDKSAIGHLLNETVGKYLPPRLKASNHQHMASLATGEYEQARFHIDQVLALNDRGYLACLERIQSHVH
FINGSWDEYTTAEDARQFRDYLPHCSFSRVEGTGHFLDLESKLAAVRVHRALLEHLLKQPEPQRAERAAGFH
EMAIGYA*
AnnotationID:2586GeneID:2578
AnnotatorUID: diggins
Modification Date/Time: 2005-04-19 11:11:29
Gene Name:rhlA
Confidence Code:1
GeneProduct:rhamnosyltransferase chain A
Cell Localization:()
Synonyms:phaG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:3-hydroxyacyl-acyl carrier protein CoA transferase
Functional Category Confidence Code:5
COGs:pfam00561,COG0596
Secondary Functional Category(ies):
EC Number:2.4.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56777.1| (AE016867) 3-hydroxyacyl-CoA-acyl carrier protein transferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 258

 Score =  243 bits (621), Expect = 7e-64
 Identities = 119/230 (51%), Positives = 161/230 (70%)

>gb|AAC34749.1| (AF052507) acyl-transferase [Pseudomonas putida]
          Length = 295

 Score =  234 bits (597), Expect = 5e-61
 Identities = 125/295 (42%), Positives = 181/295 (61%), Gaps = 3/295 (1%)






 
Structural Features:
pfam00561, Abhydrolase_1, alpha/beta hydrolase fold.
CD-Length = 224 residues,  95.1% aligned
Score = 48.6 bits (115), Expect = 9e-07
Identities = 47/215 (21%), Positives = 78/215 (36%), Gaps = 14/215 (6%)

COG0596, MhpC, Predicted hydrolases or acyltransferases 
(alpha/beta hydrolase superfamily)
CD-Length = 282 residues,  97.2% aligned
Score = 64.3 bits (154), Expect = 2e-11






   
Genomic Context:






   
Comment:


















    ReferenceID:5159




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22304




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32080




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2578_cdd.html


    		




 
URL:

















    ReferenceID:34095




  
Author/Investigator(s):
        Deziel E, Lepine F, Milot S, Villemur R.





  
Title:
        rhlA is required for the production of a novel biosurfactant promoting swarming motility in Pseudomonas aeruginosa: 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs), the precursors of rhamnolipids.




  
PubMed:
        12904540


MedLine:
        




    
Source:
Microbiology. 2003 Aug;149(Pt 8):2005-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12904540

















    ReferenceID:34096




  
Author/Investigator(s):
        Ochsner UA, Fiechter A, Reiser J.





  
Title:
        Isolation, characterization, and expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB genes encoding a rhamnosyltransferase involved in rhamnolipid biosurfactant synthesis.




  
PubMed:
        8051059


MedLine:
        




    
Source:
J Biol Chem. 1994 Aug 5;269(31):19787-95.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8051059









           
Homologs By Global Alignment 

           

       



     
Gene ID:2578
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
125709
                           gb|AAA62128.1|
                           (L28170) rhamnosyl transferase [Pseudomonas aeruginosa]
                           295
                           99.99
                           99.99
                           0
                           1523.0
     

     
     
4281
                           PA3479_tr
                           translation of PA3479
                           295
                           99.66
                           99.66
                           0
                           1515.0
     

     
     
125710
                           gb|AAG06867.1|AE004768_12
                           (AE004768) rhamnosyltransferase chain A [Pseudomonas aeruginosa PAO1]
                           295
                           99.66
                           99.66
                           0
                           1515.0
     

     
     
125712
                           dbj|BAB32432.1|
                           (AB047080) 3-hydroxyacyl-acyl carrier protein CoA transferase [Pseudomonas sp. 61-3]
                           298
                           42.61
                           61.74
                           7
                           613.0
     

     
     
125716
                           gb|AAK71349.1|
                           (AY039839) hydroxyacyl-ACP:CoA transacylase PhaG [Pseudomonas nitroreducens]
                           298
                           41.94
                           60.40
                           6
                           599.0
     

     






      

          
GC ORFID: 45580How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3479Glimmer Score: 

          
Start: 3305301Stop: 3304414

          
Length: 888


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3479 translation of PA3479Homolog Bit Score 588.0

          
Other Homologs:  psyr_15may02_Scaffold11_revised_gene1930 (ORF: BLASTX 255.0), t_PA0730 (ORF: BLASTX 233.0), t_PA3479 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGGCGCGAAAGTCTGTTGGTATCGGTTTGCAAGGGCCTGCGGGTACATGTCGAGCGCGTTGGGCAGGAT 
CCCGGGCGCAGCACGGTGATGCTGGTCAACGGCGCGATGGCGACCACCGCCTCGTTCGCCCGGACCTGCAAG 
TGCCTGGCCGAACATTTCAACGTGGTGCTGTTCGACCTGCCCTTCGCCGGGCAGTCGCGGCAGCACAACCCG 
CAGCGCGGGTTGATCACCAAGGACGACGAGGTGGAAATCCTCCTGGCGCTGATCGAGCGCTTCGAGGTCAAT 
CACCTGGTCTCCGCGTCCTGGGGCGGTATCTCCACGCTGCTGGCGCTGTCGCGCAATCCGCGCGGTATCCGC 
AGCTCGGTGGTGATGGCATTCGCCCCTGGACTGAACCAGGCGATGCTCGACTACGTCGGGCGGGCGCAGGCG 
CTGATCGAGCTGGACGACAAGTCGGCGATCGGCCATCTGCTCAACGAGACCGTCGGCAAATACCTGCCGCCG 
CGCCTGAAAGCCAGCAACCATCAGCACATGGCTTCGCTGGCCACCGGCGAATACGAGCAGGCGCGCTTTCAC 
ATCGACCAGGTGCTGGCGCTCAACGATCGGGGCTACCTGGCTTGCCTGGAGCGGATCCAGAGCCACGTGCAT 
TTCATCAACGGCAGCTGGGACGAGTACACCACCGCCGAGGACGCCCGCCAGTTCCGCGACTACCTGCCGCAC 
TGCAGTTTCTCGCGGGTGGAGGGCACCGGGCATTTCCTCGACCTGGAGTCCAAGCTGGCCGCGGTACGCGTG 
CACCGCGCCCTGCTCGAGCACCTGCTGAAGCAACCGGAGCCGCAGCGGGCGGAACGCGCGGCGGGATTCCAC 
GAGATGGCCATCGGCTACGCCTGA